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任园园, 丁子丹, 王盼, 殷林波, 傅雅静, 姜拥军, 张子宁. IL-15及其受体在HIV感染中表达及意义[J]. 中国公共卫生, 2019, 35(2): 105-108. DOI: 10.11847/zgggws1121092
引用本文: 任园园, 丁子丹, 王盼, 殷林波, 傅雅静, 姜拥军, 张子宁. IL-15及其受体在HIV感染中表达及意义[J]. 中国公共卫生, 2019, 35(2): 105-108. DOI: 10.11847/zgggws1121092
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Yuan-yuan REN, Zi-dan DING, Pan WANG, . Expression and significance of interleukin-15 and its receptor in HIV infection individuals[J]. Chinese Journal of Public Health, 2019, 35(2): 105-108. DOI: 10.11847/zgggws1121092
Citation: Yuan-yuan REN, Zi-dan DING, Pan WANG, . Expression and significance of interleukin-15 and its receptor in HIV infection individuals[J]. Chinese Journal of Public Health, 2019, 35(2): 105-108. DOI: 10.11847/zgggws1121092
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IL-15及其受体在HIV感染中表达及意义

Expression and significance of interleukin-15 and its receptor in HIV infection individuals

    摘要
  • 摘要:
      目的  研究人免疫缺陷病毒（HIV）感染者外周血单个核细胞（PBMC）中白细胞介素15（IL-15）、白细胞介素15受体 α（IL-15Rα）及解聚素-金属蛋白酶17（ADAM17）mRNA的表达，探讨其在HIV感染疾病进展中的作用。
      方法  应用实时定量PCR（RT-PCR）检测9例HIV早期感染者（感染 < 1年，EHI组）、30例慢性HIV感染者（感染≥1年，CHI组）及9例健康对照（NC组）PBMC中IL-15、IL-15Rα、ADAM-17 mRNA水平。
      结果  CHI组PBMC中IL-15 mRNA的表达明显高于NC组（P < 0.01）；EHI组及CHI组PBMC中IL-15Rα、ADAM17 mRNA的表达明显高于NC组（P < 0.05）；HIV感染者PBMC中IL-15、IL-15Rα、ADAM17 mRNA的表达与病毒载量（VL）呈正相关（P < 0.05）；HIV感染者IL-15 mRNA表达与IL-15Rα mRNA表达呈正相关（P < 0.001），IL-15Rα mRNA表达与ADAM17 mRNA表达水平呈正相关（P < 0.001）。
      结论  HIV感染后IL-15、IL-15Rα、ADAM17表达上调，皆与HIV病毒复制水平相关。

     

    Abstract:
      Objective  To study expressions of interleukin-15 (IL-15), interleukin-15 receptor α (IL-15Rα) and a disintegrin and metalloprotease domain 17 (ADAM17) in peripheral blood mononuclear cells (PBMC) from HIV-infected individuals and to explore roles of the expressions in the progression of HIV infection.
      Methods  Reverse transcription-PCR (RT-PCR) was used to detect IL-15, IL-15Rα and ADAM17 mRNA in PBMC of 9 early HIV-infected individuals (infected within one year, EHI group), 30 chronic HIV-infected individuals (infected for more than one year, CHI group), and 9 healthy controls (NC group).
      Results  The expression of IL-15 mRNA in PBMC of CHI group was significantly higher than that in NC group (P < 0.01). The expression of IL-15Rα and ADAM17 mRNA in PBMC of EHI group and CHI group was significantly higher than those in NC group (both P < 0.05). The expression of IL-15, IL-15Rα and ADAM17 mRNA in PBMC of HIV-infected individuals were positively correlated with viral load (VL) (P < 0.05). IL-15 mRNA expression in PBMC was positively correlated with IL-15Rα mRNA expression in HIV-infected individuals (P < 0.001), and IL-15Rα mRNA expression was positively correlated with ADAM17 mRNA expression (P < 0.001).
      Conclusion  HIV-infection increases the expression of IL-15, IL-15Rα, and ADAM17 in PBMCs and their expressions are related to HIV viral load in HIV-infected individuals.

     

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