Assessment on quality of complex amino acid chelated calcium and its calcium supplementation effect in rats
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摘要:
目的 以栉孔扇贝加工副产物为原料,制备复合氨基酸螯合钙,旨在获得安全稳定的新型补钙剂,实现栉孔扇贝加工副产物的高值化利用。 方法 通过重金属等有害物质检测、傅里叶红外光谱表征和体外胃肠消化系统模拟进行复合氨基酸螯合钙质量和稳定性分析;采用动物试验测定内脏指数、血清生化、骨密度等指标,评价复合氨基酸螯合钙的补钙功能。 结果 复合氨基酸螯合钙质量指标符合相关标准要求,安全稳定,易于吸收且利用率高,其钙离子表观吸收率可达(81.83 ± 8.48)%,保留率可达(81.37 ± 5.53)%,并可显著提高骨密度(P < 0.05),达到补钙效果。 结论 以栉孔扇贝加工副产物为原料制备的复合氨基酸螯合钙是一种潜在的高效补钙剂。 Abstract:Objective To prepared complex amino acid chelated calcium (CAACC) with a by-product of Chlamys farreri processing and to assess the quality and calcium supplementation effect of the prepared CAACC for effective utilization of Chlamys farreri processing by-product. Methods The quality and stability of the prepared CAACC were assessed with detections of heavy metals and other harmful substances, Fourier transform infrared spectroscopy (FTIR) and in vitro simulation of gastrointestinal digestive system. Meanwhile, the calcium supplementation effect of CAACC was evaluated with visceral index, serum biochemical index, bone mineral density and other indicators in experimental rats. Results The detection results indicated that the prepared CAACC met relevant quality standards, with high absorption and utilization rate. In experimental rats, the apparent absorption rate of calcium ions in CAACC was (81.83 ± 8.48)% and the retention rate was 81.37 ± 5.53%; furthermore, bone mineral density of the rats treated with the prepared CAACC increased significantly (P < 0.05). Conclusion The complex amino acid chelated calcium prepared with a by-product of Chlamys farreri processing is a potential and efficient calcium supplement agent. -
表 1 复合氨基酸螯合钙对大鼠内脏指数影响(
${\bar x} \pm s$ ,n = 10)组别(mg/kg) 体重(g) 内脏指数(%) 心脏 肝脏 肾脏 肺脏 脾脏 空白对照组 307.00 ± 15.72 0.31 ± 0.02 4.27 ± 0.20 0.69 ± 0.05 0.57 ± 0.05 0.20 ± 0.03 阴性对照组 345.50 ± 16.66 0.30 ± 0.02 4.09 ± 0.29 0.74 ± 0.03 0.51 ± 0.04 0.22 ± 0.02 螯合钙 100 325.00 ± 20.02 0.31 ± 0.01 3.99 ± 0.44 0.69 ± 0.05 0.52 ± 0.02 0.25 ± 0.03 200 346.00 ± 17.62 0.30 ± 0.01 3.80 ± 0.31 0.71 ± 0.05 0.55 ± 0.06 0.26 ± 0.03 300 352.00 ± 12.34 0.31 ± 0.02 3.91 ± 0.42 0.81 ± 0.05 0.53 ± 0.05 0.23 ± 0.02 碳酸钙组 100 321.00 ± 10.22 0.30 ± 0.02 4.00 ± 0.43 0.77 ± 0.04 0.55 ± 0.04 0.25 ± 0.04 表 2 复合氨基酸螯合钙对大鼠血常规指标影响(
${\bar x} \pm s$ ,n = 10)组别(mg/kg) 血钙(mmol/L) 血镁(mmol/L) 血磷(mmol/L) 碱性磷酸酶(U/L) 空白对照组 2.35 ± 0.10 0.95 ± 0.06 2.57 ± 0.40 51.69 ± 9.56 阴性对照组 2.35 ± 0.06 0.93 ± 0.05 2.26 ± 0.18 146.01 ± 13.21 螯合钙 100 2.30 ± 0.08 1.00 ± 0.08 2.35 ± 0.30 69.68 ± 5.45 ab 200 2.33 ± 0.10 1.03 ± 0.10 2.84 ± 0.46 63.61 ± 5.19 ab 300 2.48 ± 0.19 1.05 ± 0.06 3.37 ± 0.78 56.60 ± 6.10 ab 碳酸钙组 100 2.50 ± 0.44 1.00 ± 0.08 2.51 ± 0.33 102.04 ± 17.35 ab 注:与空白对照比较,a P < 0.05; 与阴性对照比较,b P < 0.05。 表 3 复合氨基酸螯合钙对钙表观吸收率和保留率影响(
${\bar x} \pm s$ ,n = 10)组别
(mg/kg)Ca2+摄入量
(mg/d)尿Ca2+
(mg/d)粪Ca2+
(mg/d)Ca2+表观吸收
(mg/d)Ca2+表观吸收率
(%)Ca2+保留量
(mg/d)Ca2+保留率
(%)空白对照组 60.12 ± 3.93 1.33 ± 0.28 34.73 ± 3.07 25.39 ± 4.84 42.23 ± 6.59 24.06 ± 4.90 40.01 ± 6.63 阴性对照组 0.86 ± 0.60 0.05 ± 0.02 0.09 ± 0.08 0.77 ± 0.54 89.53 ± 6.39 0.72 ± 0.54 83.00 ± 16.68 螯合钙 100 17.18 ± 0.26 0.08 ± 0.04 3.12 ± 1.39 14.06 ± 1.35 ab 81.83 ± 8.48 ab 13.98 ± 1.37 ab 81.37 ± 5.35 ab 200 31.81 ± 0.39 0.14 ± 0.06 6.39 ± 2.22 25.24 ± 2.28 b 79.91 ± 5.36 ab 25.10 ± 1.83 b 78.90 ± 5.35 ab 300 59.33 ± 0.35 0.21 ± 0.13 12.12 ± 2.82 47.21 ± 2.89 ab 79.57 ± 4.84 ab 47.00 ± 2.91 ab 79.21 ± 4.89 ab 碳酸钙组 100 31.80 ± 0.26 1.65 ± 0.34 20.22 ± 3.08 11.58 ± 3.02 ab 36.41 ± 9.28 ab 9.93 ± 3.06 ab 31.22 ± 9.42 ab 注:与空白对照比较,a P < 0.05; 与阴性对照比较,b P < 0.05。 -
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