1999 Vol. 15, No. 12
Display Method:
1999, 15(12): 1059-1059,1060.
Abstract:
Salvia Miltio rrhiza-phosph olipidcholice liposome was made by Salvia Miltiorr hiza. Salvia Miltiorrhiza- phosph olipidcholine liposome was added to alveolar macr ophage( AM) medium and cultured with quartz. The result showed that morphology and survival rate of AM were close to the control group, MDA content、Ca+ + concentration in AM were obviously lower than those in the quartz group, but GSH-Px activity、SOD activity and reduced GSH content were higher than those in the quartz group. Those changes suggested that Salvia Miltiorrhiza- phospho lipidcholine liposome could suppress quartz-induced lipid peroxidation, keep calcium homeostasis and pr otect AM.
Salvia Miltio rrhiza-phosph olipidcholice liposome was made by Salvia Miltiorr hiza. Salvia Miltiorrhiza- phosph olipidcholine liposome was added to alveolar macr ophage( AM) medium and cultured with quartz. The result showed that morphology and survival rate of AM were close to the control group, MDA content、Ca+ + concentration in AM were obviously lower than those in the quartz group, but GSH-Px activity、SOD activity and reduced GSH content were higher than those in the quartz group. Those changes suggested that Salvia Miltiorrhiza- phospho lipidcholine liposome could suppress quartz-induced lipid peroxidation, keep calcium homeostasis and pr otect AM.
1999, 15(12): 1066-1066,1067.
Abstract:
173 graduating students of medical university were tested with SCL -90 and life events scales, and the correlation analy sese between them was made.It showed that there was closed rela tionship between decline of psy cological health level and negative life events in the students.The result also showd that psychosocial factors was relative to some psy chological symptoms.
173 graduating students of medical university were tested with SCL -90 and life events scales, and the correlation analy sese between them was made.It showed that there was closed rela tionship between decline of psy cological health level and negative life events in the students.The result also showd that psychosocial factors was relative to some psy chological symptoms.
1999, 15(12): 1068-1069,1070.
Abstract:
Antimutagenicity effects of 16 Kinds Chinese Herbal Drugs on a dual biological indicators-fo rward (SMr-5) and reversion (Trp+)mutations induced by 4-nitroquinoline-1-o xide (4NQO) were determined by a bioantimutagenic model in Escherichia coli strain CM891, respectely.Results showed the water extract of Fructus chebulae, Radix polygoni multiflori, Fructurs lig ustri lucidi and shell of water chestnut with a pedicle could produce antimutagenisis against 4NQU-induced fo rward and reversion mutation.Futher, shell of w ater chestnut with a pedicle also could do antimutag enisis to spo ntaneous ones.However, Paeonia lactiflora could prolmote 4NQO-induced mutation.
Antimutagenicity effects of 16 Kinds Chinese Herbal Drugs on a dual biological indicators-fo rward (SMr-5) and reversion (Trp+)mutations induced by 4-nitroquinoline-1-o xide (4NQO) were determined by a bioantimutagenic model in Escherichia coli strain CM891, respectely.Results showed the water extract of Fructus chebulae, Radix polygoni multiflori, Fructurs lig ustri lucidi and shell of water chestnut with a pedicle could produce antimutagenisis against 4NQU-induced fo rward and reversion mutation.Futher, shell of w ater chestnut with a pedicle also could do antimutag enisis to spo ntaneous ones.However, Paeonia lactiflora could prolmote 4NQO-induced mutation.
1999, 15(12): 1074-1074,1075.
Abstract:
Effects of noise on cellar immune function in mouse which exposed to noise( the intensity was simulated noise work, 105dB(A), 90dB(A), 75dB(A) respectively ) was determined by the method of immunotoxicology. The results have been demonstrated that the stress induced showed a significant decrease in proliferativ eability of lymphocyte. But t he activity of IL- 2 had no signif-icant change. Pr eliminary estimates that noise had potential immune supression.
Effects of noise on cellar immune function in mouse which exposed to noise( the intensity was simulated noise work, 105dB(A), 90dB(A), 75dB(A) respectively ) was determined by the method of immunotoxicology. The results have been demonstrated that the stress induced showed a significant decrease in proliferativ eability of lymphocyte. But t he activity of IL- 2 had no signif-icant change. Pr eliminary estimates that noise had potential immune supression.
1999, 15(12): 1076-1078.
Abstract:
Object To investig ate the rural population' s health behavior status and the factors associated with ill-behavio r.Method Cross -sectional study.Results I n men, the rate of smoking and drinking were 46.3 %, 46.9 % respectively.Smoking behavior was related to drinking's significantly.8.3% of all subjects did not eat reg ular three meals daily and 89.1% in tw o-meals subjects did not eat reg ularbreakfasts.Never or rarely-exercise was 90.4%, 74.9 % of all subjects sleeped seven to eight hours per day.Only 38.5% of the respondents had the habit of washing hands before lunch and after W.C.79.3% of the subjects brushed their teeth daily everyday.Comprehensive health behavior status analysis illustrated:64.8 % of the subjects had 3 ~ 5 health behavior.Only 16.9 % had more than six.Conclusion The status of health behavior of rural population were bad.We must reinfo rce the health educatio n in rural community
Object To investig ate the rural population' s health behavior status and the factors associated with ill-behavio r.Method Cross -sectional study.Results I n men, the rate of smoking and drinking were 46.3 %, 46.9 % respectively.Smoking behavior was related to drinking's significantly.8.3% of all subjects did not eat reg ular three meals daily and 89.1% in tw o-meals subjects did not eat reg ularbreakfasts.Never or rarely-exercise was 90.4%, 74.9 % of all subjects sleeped seven to eight hours per day.Only 38.5% of the respondents had the habit of washing hands before lunch and after W.C.79.3% of the subjects brushed their teeth daily everyday.Comprehensive health behavior status analysis illustrated:64.8 % of the subjects had 3 ~ 5 health behavior.Only 16.9 % had more than six.Conclusion The status of health behavior of rural population were bad.We must reinfo rce the health educatio n in rural community
1999, 15(12): 1079-1080.
Abstract:
To probe.sex behavior characteristics and AIDS related knowledge attitudes and behaviors among drug users in Lanzhou city.Methods An anonymous investigation was carried out at adeto xification camp in Lanzhou city.Results 90.9% drug users had sex experience, 42.0% married drug users had ex tramarital sex, and 2.9 % drug users had homosexuality.57.1 % drug users were inhalers, 2.2% were injectors,and 10.8% of them took drug by both smoking and injection.29.5 % drug users took drug with regular people and 27.3% with irregular people.21.0 % injectors had used others syringes.Only 6.3% of them thought they might get AIDS virus infected.Only 41.7% of them thoug ht they would give up smoking when out of the detoxification camp.Co nclusions Because of the bad knowledg e, attitudes and behaviors on AIDS among drug users, Prev ention program should be canr red out urgently in Lanzhou city.
To probe.sex behavior characteristics and AIDS related knowledge attitudes and behaviors among drug users in Lanzhou city.Methods An anonymous investigation was carried out at adeto xification camp in Lanzhou city.Results 90.9% drug users had sex experience, 42.0% married drug users had ex tramarital sex, and 2.9 % drug users had homosexuality.57.1 % drug users were inhalers, 2.2% were injectors,and 10.8% of them took drug by both smoking and injection.29.5 % drug users took drug with regular people and 27.3% with irregular people.21.0 % injectors had used others syringes.Only 6.3% of them thought they might get AIDS virus infected.Only 41.7% of them thoug ht they would give up smoking when out of the detoxification camp.Co nclusions Because of the bad knowledg e, attitudes and behaviors on AIDS among drug users, Prev ention program should be canr red out urgently in Lanzhou city.
1999, 15(12): 1081-1082,1083.
Abstract:
The purpose of this study is to explore the Lanzhou newbom blood lead level and its relative defermination factors.We conducted ear lobe microblood obstetrical department of our hospital.Using graphitic oven atomic abso rption spectrometry collecting 0.1ml peripheraL blood for measurement.the statical disposal was made by using multiple variables progressive regressive analyses. Result :blood lead Level mean 83.5μg/ L abno rmal detedable rate is 24.27 percent ;the premature infant, low weight infant and infant w ho had history of asphy xia, their blood lead level almost increased; Mother' s blood lead, cultural degree, occupation and some environmental factors had relativity with new born blood lead level.Conclusion :The mean of the city newborn blood lead level is 83.5μg/ L, that signilicantly higher than other provinces and cities, the cases of ≥100μg/ L reached 24.27 percent.Preventing and treating lead poisoning by heathy teaching, environmental intervention, clinical prev ention and treatment.
The purpose of this study is to explore the Lanzhou newbom blood lead level and its relative defermination factors.We conducted ear lobe microblood obstetrical department of our hospital.Using graphitic oven atomic abso rption spectrometry collecting 0.1ml peripheraL blood for measurement.the statical disposal was made by using multiple variables progressive regressive analyses. Result :blood lead Level mean 83.5μg/ L abno rmal detedable rate is 24.27 percent ;the premature infant, low weight infant and infant w ho had history of asphy xia, their blood lead level almost increased; Mother' s blood lead, cultural degree, occupation and some environmental factors had relativity with new born blood lead level.Conclusion :The mean of the city newborn blood lead level is 83.5μg/ L, that signilicantly higher than other provinces and cities, the cases of ≥100μg/ L reached 24.27 percent.Preventing and treating lead poisoning by heathy teaching, environmental intervention, clinical prev ention and treatment.
1999, 15(12): 1084-1085.
Abstract:
To explore the ergulation of hea t shock protein 27(HSP27) in case of marching with 15kg in dry-heat desert, the plasmas HSP27 of 56 marchers and 14 controls were detected.The results revealed that there is sig nificant difference between exposed g roups and control(P<0.01), and between for 1 hour and 2 or 3 hours at 3.5km/ h(P <0.05), but there is no significant difference between different time exposures at 5km/h, a t 3.5km/ hand 5km/ h for the same time.It w as in the first 2 hours that HSP27 w as expressed the most activ ely, and in he second hour that HSP27 degraded the most quickly.After marching for 2 hours, a new balance between the synthesis and consumption of HSP27 regained.
To explore the ergulation of hea t shock protein 27(HSP27) in case of marching with 15kg in dry-heat desert, the plasmas HSP27 of 56 marchers and 14 controls were detected.The results revealed that there is sig nificant difference between exposed g roups and control(P<0.01), and between for 1 hour and 2 or 3 hours at 3.5km/ h(P <0.05), but there is no significant difference between different time exposures at 5km/h, a t 3.5km/ hand 5km/ h for the same time.It w as in the first 2 hours that HSP27 w as expressed the most activ ely, and in he second hour that HSP27 degraded the most quickly.After marching for 2 hours, a new balance between the synthesis and consumption of HSP27 regained.
1999, 15(12): 1094-1095.
Abstract:
In order to describe the situation of tuberculosis infection in army, to provide reference for the control of tuberculosis.We have done PPD skin tests for 15469 armymen.The results show total positive rate is 34.31 persent.Strong positive rate is 3.21 persent. Compared with the results of the national survey in 1980, the po sitive rate dropped 44.96 persent.There are more distinguish among different army, including member, place w here troops rate dropped 44.96 persent.There are more distinguish among different army,including member, place where troops are stationed, and task to be charged.Patients in army, epidemic situation in the stationed places and intensity of training are the main facto rs for the increasing morbidity rate, which are also the emphasis to control.
In order to describe the situation of tuberculosis infection in army, to provide reference for the control of tuberculosis.We have done PPD skin tests for 15469 armymen.The results show total positive rate is 34.31 persent.Strong positive rate is 3.21 persent. Compared with the results of the national survey in 1980, the po sitive rate dropped 44.96 persent.There are more distinguish among different army, including member, place w here troops rate dropped 44.96 persent.There are more distinguish among different army,including member, place where troops are stationed, and task to be charged.Patients in army, epidemic situation in the stationed places and intensity of training are the main facto rs for the increasing morbidity rate, which are also the emphasis to control.
1999, 15(12): 1096-1097.
Abstract:
A 1:1 matched case -control study w as carried out tQ reveal the factors influencing the occurrence of HA in Jiaxins citv, Zhejiang province.The multivariate analvsis in a 1:1 conditional Logistic regressio n model w as used.The results show ed that the following risk facto rs affected Hepatitis A :contacting with patient of HA, eating outside, poor economic condition, poor health condition in eating place and poor sanitary facilities of household.The OR walues of risk factors above are in descending order.The Susgestion are made whlch ars to direct against those contro lable factors.
A 1:1 matched case -control study w as carried out tQ reveal the factors influencing the occurrence of HA in Jiaxins citv, Zhejiang province.The multivariate analvsis in a 1:1 conditional Logistic regressio n model w as used.The results show ed that the following risk facto rs affected Hepatitis A :contacting with patient of HA, eating outside, poor economic condition, poor health condition in eating place and poor sanitary facilities of household.The OR walues of risk factors above are in descending order.The Susgestion are made whlch ars to direct against those contro lable factors.
1999, 15(12): 1098-1099.
Abstract:
A sample of 57014 rural children aged 1~ 8 years old w as selected rom three natdural g eog raphic areas in Anhui province with multistage sampling.All subjects were screened to diagnosis cerebral palsy.The results showed tha t the prevalence of cerebral palsy was 2.07 per 1000.Thee was no sig nificant difference in the prevalenc between males and females.The sig nificant difference in the prevalence was not found in different age g roups.The area distribution of cerebral palsy w as different in the province.The prevalences in south areas of Yangzi River, the areas between Yang zi River and Huaihe River, and the nor th areas of Huaihi River were 3.34 per 1000, 2.09 per 1000, and 1.28 per 1000, respectively.I t is necessary to further disclose the causes of the area distributive features of cerebral palsy.
A sample of 57014 rural children aged 1~ 8 years old w as selected rom three natdural g eog raphic areas in Anhui province with multistage sampling.All subjects were screened to diagnosis cerebral palsy.The results showed tha t the prevalence of cerebral palsy was 2.07 per 1000.Thee was no sig nificant difference in the prevalenc between males and females.The sig nificant difference in the prevalence was not found in different age g roups.The area distribution of cerebral palsy w as different in the province.The prevalences in south areas of Yangzi River, the areas between Yang zi River and Huaihe River, and the nor th areas of Huaihi River were 3.34 per 1000, 2.09 per 1000, and 1.28 per 1000, respectively.I t is necessary to further disclose the causes of the area distributive features of cerebral palsy.
1999, 15(12): 1101-1103.
Abstract:
Lead in foods is a main source of lead intakes in children.In order to study how dietary factors affect lead metabolism, Lead concentrations in some kinds of foods were determined, intakes of lead and other nutrients from foods w ere calculated and analy zed in 44 preschool children aging from 5 to 7 years old in Nanto ng city. Lead concentrations of 143 kinds of foods ex amined in the study were under permitted level of lead in foods.Traditional techno logical process affected lead concentrations in foo ds a lo t.Lead intakes from foods of those w hose lead concentration in hair are below 10μg/g (group A)w ere 185.25 ±42.85μg/ g, higher than 160.91±31.47μg/ g of those w hose lead concentration in hair are above 10 μg/ g (group B)(P <0.05).Dietary lead intakes of the two groups w ere all ex cess the limit recommended by FAO/ WHO in 1986.Average daily intakes of dietary calcium, retinol, vitamin E, dairy and veg etables of g roup A were hig her than that of group B (P <0.05).It was suggested that those dietary factors might be pro tective facto rs of the damage of lead in preschool children.
Lead in foods is a main source of lead intakes in children.In order to study how dietary factors affect lead metabolism, Lead concentrations in some kinds of foods were determined, intakes of lead and other nutrients from foods w ere calculated and analy zed in 44 preschool children aging from 5 to 7 years old in Nanto ng city. Lead concentrations of 143 kinds of foods ex amined in the study were under permitted level of lead in foods.Traditional techno logical process affected lead concentrations in foo ds a lo t.Lead intakes from foods of those w hose lead concentration in hair are below 10μg/g (group A)w ere 185.25 ±42.85μg/ g, higher than 160.91±31.47μg/ g of those w hose lead concentration in hair are above 10 μg/ g (group B)(P <0.05).Dietary lead intakes of the two groups w ere all ex cess the limit recommended by FAO/ WHO in 1986.Average daily intakes of dietary calcium, retinol, vitamin E, dairy and veg etables of g roup A were hig her than that of group B (P <0.05).It was suggested that those dietary factors might be pro tective facto rs of the damage of lead in preschool children.
1999, 15(12): 1108-1110.
Abstract:
To learn the rela tionship betw een food structure and dental fluorosis, caries among Xiaerba children in Tibet, we investig ated the Xiaerba and Tibetan children living in Zhangmu town under Mount Qomolangma.The methods of Horowitz' s Tooth surface Index of Fluorosis, caries published by WHO and to tal intake of fluoride published by the WHO/ FAO were used.The results shown that the prevalence of dental fluorosis was lowest among Xiaerba and Tibetan children(37.40% and 25.86%, respectively)in all areas with brick tea type dental fluorosis of China.The caries incidence means of caries in Xiaerba children (28.46%, 0.62)were lower than that of Tibe tan children (62.07 %, 2.17).The change of food structure among children lived in Zhangmu tow n induced decreasing of fluo ride intake and increasing the absorption of much more nutritio n, and enhanced the human abilityag ainst fluorosis.The reason of lower caries prevalence in Xiaerba children may come from stable and rational food strucfure, and higher caries prevalence in Tibe tan children due to much moreconsumption of refined food and sucrose.I f Xiaerba Children cut down appropriately intake of Zanba and milk tea(made from brick tea), it may supply a mode of reducing the harm of fluorosis and caries in prevalence areas of brick tea type fluorosis.
To learn the rela tionship betw een food structure and dental fluorosis, caries among Xiaerba children in Tibet, we investig ated the Xiaerba and Tibetan children living in Zhangmu town under Mount Qomolangma.The methods of Horowitz' s Tooth surface Index of Fluorosis, caries published by WHO and to tal intake of fluoride published by the WHO/ FAO were used.The results shown that the prevalence of dental fluorosis was lowest among Xiaerba and Tibetan children(37.40% and 25.86%, respectively)in all areas with brick tea type dental fluorosis of China.The caries incidence means of caries in Xiaerba children (28.46%, 0.62)were lower than that of Tibe tan children (62.07 %, 2.17).The change of food structure among children lived in Zhangmu tow n induced decreasing of fluo ride intake and increasing the absorption of much more nutritio n, and enhanced the human abilityag ainst fluorosis.The reason of lower caries prevalence in Xiaerba children may come from stable and rational food strucfure, and higher caries prevalence in Tibe tan children due to much moreconsumption of refined food and sucrose.I f Xiaerba Children cut down appropriately intake of Zanba and milk tea(made from brick tea), it may supply a mode of reducing the harm of fluorosis and caries in prevalence areas of brick tea type fluorosis.
1999, 15(12): 1113-1114.
Abstract:
The tox icity and ant-i tumor activity of Momor dica charatia L. were studied. T he r esults showed the acute LD50 value of Momordica charatia L. jiuce in gastr ic( ig) was??20mg/ kg and LD50=296??3mg/ kg injected intr aper itoneally. The juice of Momor dica charatia L. didn't show any inhibitor y action to S- 180 byig, but the ratio of inhibit ion w as 39. 3% by ip. On the contrar y, the extract of Momordica char at ia L. residue w hich were extr acted by a-lcohol didn't show inhibitory action to S- 180 by ip, but did byig, and the ratio of inhibition was up to 37. 1%.
The tox icity and ant-i tumor activity of Momor dica charatia L. were studied. T he r esults showed the acute LD50 value of Momordica charatia L. jiuce in gastr ic( ig) was??20mg/ kg and LD50=296??3mg/ kg injected intr aper itoneally. The juice of Momor dica charatia L. didn't show any inhibitor y action to S- 180 byig, but the ratio of inhibit ion w as 39. 3% by ip. On the contrar y, the extract of Momordica char at ia L. residue w hich were extr acted by a-lcohol didn't show inhibitory action to S- 180 by ip, but did byig, and the ratio of inhibition was up to 37. 1%.
1999, 15(12): 1124-1126.
Abstract:
In order to understand the rising and falling trend o f the reported incidence rate and the reported morality rate of the lawful infectious diseases in Guang xi during 1950 ~ 1998, we analysed systematically the data collected during the oeriod with the descriptive epidemiology.We described the changing characteristic of the teported incidence rate and the repo rted mo rtality tate of the diseases.We analysed the repo rted incidence rate and the propo rtional mo rtality rate of each disease in different vears.We also analysed the changing characteristic and it' s Qause of several major infectious diseases.We discuss the problems and the basic strateg y o f Infectious disease control w lth w hich Guang xi will befaced in the 21th century.We bring forw ard soma new views.
In order to understand the rising and falling trend o f the reported incidence rate and the reported morality rate of the lawful infectious diseases in Guang xi during 1950 ~ 1998, we analysed systematically the data collected during the oeriod with the descriptive epidemiology.We described the changing characteristic of the teported incidence rate and the repo rted mo rtality tate of the diseases.We analysed the repo rted incidence rate and the propo rtional mo rtality rate of each disease in different vears.We also analysed the changing characteristic and it' s Qause of several major infectious diseases.We discuss the problems and the basic strateg y o f Infectious disease control w lth w hich Guang xi will befaced in the 21th century.We bring forw ard soma new views.
1999, 15(12): 1131-1133.
Abstract:
OBJECTIVE To measure the antibio tic susceptibility ofneisser ia gonorrhoeae epidemic strains in Zhanjiang(Guangdong).METHODS 98 gonococcal isolated obtained from 1998 through 1999 in Zhanjiang were tested for theirantibio tic susceptibilities based on the systemic identification.The inhibitory zo ne diameters (mm)and the minimum inhibito ry concenrations(MICs)of penicillinte tracycline, spectinomy cin, ceftr iaxone and ciprofloxacin were determined by using disk diffusion and agar dilution methods, respectively.Then difined the susceptibilities of all these isolates using the MCCLS criteria.RES ULTS The percentages of gonococci resitant strains to penicillin, tetracycline, spectinomy cin,ceftriaxion and ciproflox acin were 32.65%、69.39 %、8.16%、 13.27 % and 82.65 % by using disk diffusion metod.At the same tiome, the percentages of resistant strains by using ag ar dilutiion method were 23.91 %、49.46 %、11.11% 、16.48% and 59.34 % respectiv ely.CONCLUSION The resistant strains of contemporary gonococci in Zhanjiang were serious.especially the ciprofloxacin resistance. By the way.contimued active survillance is needed to detect and contro l the spread of ceftr iaxo ne and spectinomycin resistant Neisseria g onor rhoeae.Strains to Antibiotics.
OBJECTIVE To measure the antibio tic susceptibility ofneisser ia gonorrhoeae epidemic strains in Zhanjiang(Guangdong).METHODS 98 gonococcal isolated obtained from 1998 through 1999 in Zhanjiang were tested for theirantibio tic susceptibilities based on the systemic identification.The inhibitory zo ne diameters (mm)and the minimum inhibito ry concenrations(MICs)of penicillinte tracycline, spectinomy cin, ceftr iaxone and ciprofloxacin were determined by using disk diffusion and agar dilution methods, respectively.Then difined the susceptibilities of all these isolates using the MCCLS criteria.RES ULTS The percentages of gonococci resitant strains to penicillin, tetracycline, spectinomy cin,ceftriaxion and ciproflox acin were 32.65%、69.39 %、8.16%、 13.27 % and 82.65 % by using disk diffusion metod.At the same tiome, the percentages of resistant strains by using ag ar dilutiion method were 23.91 %、49.46 %、11.11% 、16.48% and 59.34 % respectiv ely.CONCLUSION The resistant strains of contemporary gonococci in Zhanjiang were serious.especially the ciprofloxacin resistance. By the way.contimued active survillance is needed to detect and contro l the spread of ceftr iaxo ne and spectinomycin resistant Neisseria g onor rhoeae.Strains to Antibiotics.
1999, 15(12): 1138-1138.
Abstract:
The two intravenous tox icity data for sodium valpro ate in mice, generated by using classical LD50 test, were compared.Though there were g reat differences betw een the tw o acute tox ic-itytest in testing time, bo dy weig ht, dosage, class interv al, and death distribution etc. the two LD50 values were very close ( 1.14and 0.96 g/ kg ). It indicated that LD50 value as an evaluation index is st ill steady and repr esentative.
The two intravenous tox icity data for sodium valpro ate in mice, generated by using classical LD50 test, were compared.Though there were g reat differences betw een the tw o acute tox ic-itytest in testing time, bo dy weig ht, dosage, class interv al, and death distribution etc. the two LD50 values were very close ( 1.14and 0.96 g/ kg ). It indicated that LD50 value as an evaluation index is st ill steady and repr esentative.
1999, 15(12): 1148-1149.
Abstract:
For obtaining the reombinant diagnostic antigen of Hantavirus,pBV220 -H1.3S was constructed which contained gene segment coding the Nucleocapsidpro tein (NP) o f hantan virus strain 76118.After this plasmid was transformed to and expressed in E.coli, It was found by SDS -PAGE that a new protein emerging in the baoteria cells with the Molecular Weight of about 48KD which is consistent with that of NP.Through purification of inclusion body, the recombinant NP (rNP)was used as coating antigen in Enzyme linked Immuno - sorbent Assay, detecting the specific IgG in 48 sera of suspect patients of Haemorr hagic Fever with Renal Syndrome.The result coincided with tha t by indirect immuno-fluorescent Assay by percentag e of 100, 92.88 and 93.75 for neg ative, positiv e and all samples respectively.The da ta indicated that this rNP was effective and valuable for use as diagno stic antigen.
For obtaining the reombinant diagnostic antigen of Hantavirus,pBV220 -H1.3S was constructed which contained gene segment coding the Nucleocapsidpro tein (NP) o f hantan virus strain 76118.After this plasmid was transformed to and expressed in E.coli, It was found by SDS -PAGE that a new protein emerging in the baoteria cells with the Molecular Weight of about 48KD which is consistent with that of NP.Through purification of inclusion body, the recombinant NP (rNP)was used as coating antigen in Enzyme linked Immuno - sorbent Assay, detecting the specific IgG in 48 sera of suspect patients of Haemorr hagic Fever with Renal Syndrome.The result coincided with tha t by indirect immuno-fluorescent Assay by percentag e of 100, 92.88 and 93.75 for neg ative, positiv e and all samples respectively.The da ta indicated that this rNP was effective and valuable for use as diagno stic antigen.