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2002 Vol. 18, No. 5

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2002, 18(5): 513-513. doi: 10.11847/zgggws2002-18-05-01
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2002, 18(5): 514-515. doi: 10.11847/zgggws2002-18-05-02
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2002, 18(5): 515-515. doi: 10.11847/zgggws2002-18-05-03
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2002, 18(5): 516-517. doi: 10.11847/zgggws2002-18-05-04
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2002, 18(5): 518-519. doi: 10.11847/zgggws2002-18-05-05
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2002, 18(5): 520-521. doi: 10.11847/zgggws2002-18-05-06
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2002, 18(5): 522-523. doi: 10.11847/zgggws2002-18-05-07
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2002, 18(5): 524-526. doi: 10.11847/zgggws2002-18-05-08
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2002, 18(5): 527-527. doi: 10.11847/zgggws2002-18-05-09
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2002, 18(5): 528-528. doi: 10.11847/zgggws2002-18-05-10
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Sequences Analysis of Complete S gene of Hantavirus E-142 Strain from Yunnan E.eleusis
YAO Zhi-hui, DONG Guan-mu, YU Yong-xin
2002, 18(5): 529-530. doi: 10.11847/zgggws2002-18-05-11
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ObjectiveFor understanding the molecular characteristics of Hantavirus E-142 strain isolated from Yunnan E.Eleusis.MethodsThe S complete gene was amplified by RT-PCR and the purified products was cloned into Tvector.ResultsComplete S segment of E-142 was 1 702 nucleotides in length encoding 429 amino acids.It showed 86.6%-90.4% homology with HTN virus,but only 68.3%-69.6% homolgy with SEO viruses at nucleotide level.ConclusionThe results suggested that the E-142 strain was a new subtype of HTN virus.
Studies on Method of Administration with Selenium and Cadmium by Multiple Injection
WU Zhi-gang, LI Yun, CHEN Xue-ming
2002, 18(5): 531-532. doi: 10.11847/zgggws2002-18-05-12
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ObjectiveTo study the pre-chemical reaction between Se and Cd by multiple injection administration of Se and Cd in the abdominal cavity of rats.MethodsTo deter mine the situation of poisionous stuffmixing and reaction by using the prevention of Se to the toxicity of Cd in different administration groups,together with the experiment of external chemical raction of Se-Cd and internal mix ing of I2-starch within abdominal cavity.ResultsSe and Cd injected simultaneously in abdominal cavity from right and left site could be mixed very quickly and CdSeO3 was formed by the chemical reaction.Due to the low solubility of CdSeO3,Cd absorbed by rats was remarkably decreased and the pseudo-prevention effect was observed.ConclusionAvoiding the internal pre-chemical reation while two or more test agents are administrated by injection.
Cloning and Expression of Variable Domain (V+) Gene of Surface Protein of Streptococcus Mutans
GAN Xiao-rong, WANG Xiao-qin, Michel Angelo Sciotti
2002, 18(5): 533-534. doi: 10.11847/zgggws2002-18-05-13
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ObjectiveTo isolate variable domain(V+)gene of the surface protein of streptococcus mutans and construct an expressive clone of V+gene.MethodsV+do main was amplified from gene sr by poly merase chain reaction(PCR)and subcloned into the plasmid vector pCR2.1 and the expressive plasmid vector pET21a(+).The recombinant was induced by IPTG to express target protein which was analysed by SDS-PAGE Western blotting.ResultsThe specific product of PCR was about 1.16bp which was subcloned into vectors.The recombinant clone pCVf and the recombinant expressive clone pSRVf were obtained.pSRVf expressed specific protein with a molecular weight of 43kDa.This recombinant protein could be specifically recog nized by anti Ⅰ/Ⅱ serum.ConclusionV+domain of sr gene was cloned successfully.
Analysis on S-gene Sequence of Patients with Positive HBsAg and Anti-HBs Simultaneously
DONG Jing, SHI Shuang-shuang, ZHANG Guo-qing
2002, 18(5): 535-537. doi: 10.11847/zgggws2002-18-05-14
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ObjectiveTo clarity the character istics of mutations in HBV Snucleotides and amino acid sequences from the patients with chronic HBV infection,whose serum were detected both HBsAg and anti-HBs positive.MethodsA set of specific primers were synthesized according to HBV DNA sequence of adr subtype,the whole S,gene was amplified from the serum of two patients and the PCR products were subcloned into pGEMT easy vectors.Sequence comparison was made between the target sequence from the HBsAg/anti-HBs positive serum and that from HBsAg positive serum.ResultsSequencing results proved the high consistency,with only 4 out of 1428 nt(0.3%)were different between the target sequence from the double positive serum and that from the HBsAg positive serum,but with no specific amino acid site difference in PreS1,PreS2 and major protein.There were 3 sites of difference in spacer region corresponding to HBV polymerase.Conclusion There were HBV quasispecies in the patients with chronic HBV infection.The phenomenon of both HBsAg and antiHBs positive could not be attributed to mutation in HBV genome.
Study on Expression of PCNA and p121waf1 in Course of MC Promoting Liver Tumor
HU Zhi-jian, CHEN Hua, LI Yi-wei
2002, 18(5): 538-540. doi: 10.11847/zgggws2002-18-05-15
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ObjectiveTo explore the molecular mechanism of MC in the course of MC promoting liver tumor.MethodsTwo-stage-medium-term theory were applied to set up the animal model,and biochemistry technique to evaluate the model,and then immunohistochemical technique and image analysis were used to study the expression of the PCNA and p21waf1 in the course of promoting tumor.Results(1)MC could enhance the positive reaction ration of γ-GT.(2)MC could increase the expression of PCNA.(3)MC could decrease the expression of p21waf1.Conclusion(1)MC can promote liver tumor strongly.(2)The expression change of PCAN and p21waf1 played an important role in the course of MC promoting liver tumor.
2002, 18(5): 540-540. doi: 10.11847/zgggws2002-18-05-16
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Drop-in, Drop-out Heminested PCR for HBV DNA
XIAO Sheng-xiang, YAN Hu-ling, WU Yan-hong
2002, 18(5): 541-542. doi: 10.11847/zgggws2002-18-05-17
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ObjectiveTo eliminate the opportunity of cross-contamination resulting from the two rounds of preparation of reaction mixtures in the detection of HBV DNA by conventional nested PCR and to improve PCR specificity and sensitivity.MethodsA drop-in,drop-out heminested PCR assay has been developed for the detection of HBV DNA by designing the different length and reaction concentration of primers and different annealing temperatures of initial and secondary PCR amplification.3 Primer s were all present in the initial reaction mix and the nested PCR were completed in a single tube.ResultsThe drop-in,drop-out heminested PCR is capable of amplifying 4 molecules of cloned HBV DNA to levels detectable in ethidium bromid-estained agarose gels.Of 16 specimens which were negative of HBV detected by ELISA and conventional PCR,2 were confirmed HBV DNA positive by drop-in,drop-out heminested PCR.ConclusionThe drop-in,drop-out heminested PCR for HBV achieves the sensitivity of nested PCR in a simplified format that avoids the false-positive results associated with the conventional nested PCR.The positive rate of HBV detection in clinical specimens could be elevated by the combined use of ELISA and drop-in,drop-out heminested PCR,which is of significance in decreasing the HBV infection by transfusion.
Sequencing and Genotyping for TTV in Partial Healthy Population of Shanghai
WENG Kang-sheng, GUAN Sheng-fan, ZHANG Yi-hua
2002, 18(5): 543-544. doi: 10.11847/zgggws2002-18-05-18
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ObjectiveTo investigate the TTV genoty pes distribution in healthy population of shanghai.MethodsTTV DNA in serum was detected by semi-nested PCR.Partial gene of TTV was sequenced directly from the PCRproducts,and their genetic variation was analyzed.ResultsThere were 21 positive cases in 100 serum samples from healthy population.Fourteen positive samples were sequenced.Sequencing analysis show ed that 96.2%-99.5% of nucleotides were analogous between 10 cases and TA 278,CMR 12,66.2%-67.6% between another 4 cases and the two strains,but 81.9%-84.8% in the 4 cases with TTV G4 strains.The most analogous to the 4 cases in TTV strains reported in China was 61.SEQ,and the analogous rate was 81.9%-84.3%.ConclusionThe prevalance of TTV was high in Shanghai healthy population.TTV G1a was detected as main subg enotype and a subgenotype of TTV G4 new reported in China distributin Shanghai healthy population.
Study on Isolation, Characteristics and Comparison of Several Detection Methods of Helicobacter Pylori
DING yun-fei, LI An-ming, LI De-hua
2002, 18(5): 545-547. doi: 10.11847/zgggws2002-18-05-19
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ObjectiveTo study the isolation,characteristics and comparison of several detection methods of Helicobacter pylori.MethodsAnaerobic cultivation and PCR were used to isolate and determine H.Pylori preliminarily.One strain was further determined by various tests of characteristics.ResultsAbout forty strains of H.pylori were isolated and preliminarily identified from gastric mucosa biopsy specimens in 106 patients presenting for gastroscopy athsopitals.HP-WGM was gram-negative,curved bacilli which ha smooth coats,several sheathed single polar flagella.After 2~3 days'incubation,it grew evidently as about 1mm in diameter,round,protruding,uniform in edge,semi-translucent coloies on urea agar rolled-tubes incubated at 37℃ under microaerophilic condition.It was positive in urease,catalase,oxydase tests and negative in H2S producing and gelatin liquefaction tests.It couldn't reduce NO3-,could tolerate 3.5% NaCl and 1% glycine and hydrolyze starch.The organism is immunogenic.ConclusionHP-WGM could be used as an antigen to carry out ELISA for determining the extent of H.pylori infection.At least over two methods are necessary to diagnose the infection.But in scientific research,the culture and determination of bacteria is the most reliable way.
2002, 18(5): 548-548. doi: 10.11847/zgggws2002-18-05-20
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Antibiotic Resistance Analysis for Coagulase-Negative Staphylococci Which Incubated Bacterial Vaginosis
YUAN Chun-lei, CHEN Qun, LI Guo-ming
2002, 18(5): 549-550. doi: 10.11847/zgggws2002-18-05-21
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ObjectiveTo study the antibiotic resistance for Coagulase-Negative Staphy lococci and its relation with Bacterial Vaginosis.MethodsBacteria were isolated by High Salt Mannitol medium.Resistance to antibiotic was detected by discagar diffusion method.ResultsIn the 18 strains of Coagulse-Neg ative Staphy lococci,the resistance to Pencillin-G and Sulfamethoxazole was 94.4%,the resistance to Erythromycin and Ampicillin was severe,but all susceptible to Vancomycin and Rifampicin.Multiply antibiotic resistance was 88.8%.ConclusionThe alternation of antibiotic resistance of Coagulase-Negative Staphylococci may be responsible for its correlation with Baterial Vaginosis.
Study on Change of Humoral and Cellular Immune Level of HBV Carriers
HUANG Jing-sheng, ZHANG Hui-min, CHEN Hui-zhen
2002, 18(5): 551-552. doi: 10.11847/zgggws2002-18-05-22
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ObjectiveTo study on the change of humoral and cellular immune of HBV carriers.Methods50 HBV carriers were screened by ELISA,whose HBsAg were positive and the indexes of liver functions are normal;IgG,IgA,IgM,IgE in serum were detected by immune globins detection kits and the subg roups of Timmune cell detected by APAAPmethod.ResultsThe levels of IgG,IgA,IgM of HBV carrier were significant lower than those of the no rmal control group(P<0.05), and the difference of IgE level was not significant.The levels of CD3+ and CD8+ were all significanthigher than those of the normal control group(P<0.01),however the CD4+/CD8+ was lower than that of the nor mal group(P<0.05).ConclusionThe humoral immune of HBV carriers was lower.HBV virus can stimulate the proliferation of CD4+ and CD8+,and lead to the function of celluar immune disorder.
2002, 18(5): 552-552. doi: 10.11847/zgggws2002-18-05-23
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Observation on Effeets of Prevention and Control on Tsutsugamushi Disease in Epidemic Focus
ZHOU Li-qiang, LI Chun-ming, CHEN Su-liang
2002, 18(5): 553-554. doi: 10.11847/zgggws2002-18-05-24
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ObjectiveT o evaluate effects of preventive measures on tsutsugamushi disease in a epidemic focus,Hebei province.MethodsIn the epidemic focus,the synthetical preventive measures was carried out such as extinguish rat,health education,medical training,preventive take-medicine and so on.Effects of above-mentioned measures was evaluated.ResultsRat density dropped clearly.Popularization rate of knowledge in preventive tsutsugamushi disease was 90.18% in local residents.Correct rate of clinical diagnose raised from 0 to 92.3%.Complication rate dropped from 10.5% to 0.Average treatment fare of a case dro pped more than 90%.It was found that crowd morbidity dropped clearly(P<0.01)through follow investigation for three years.ConclusionSynthetical preventive measures produced a good epidemiological effects.
2002, 18(5): 554-554. doi: 10.11847/zgggws2002-18-05-25
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Studies on Protective Immune Mechanism of Recombinant 31/32kDa Schistosoma Japonicum Antigen
SHEN Ding-wen, LUO Jin-ping, CHEN Xi-gui
2002, 18(5): 555-556. doi: 10.11847/zgggws2002-18-05-26
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ObjectiveTo study the effect of antibody in the protective immune mechanism of recombinant 31/32kDa schistosoma japonicum antigen(rSj31/32).MethodsRabbits were vaccinated multiply with rSj31/32,of which the level of IgGantibody in serum was observed dynamically.At the same time,sera with rSj31/32 vaccinated rabbits(rSj31/32-VRS) and IRS transfered passively to mice at different time to detect the protecting resistant.ResultsThe passive transfer of rSj31/32-VRS before challenge could induce 32.6% worm reduction rate and 65.0% egg reduction rate,higher than that after challenge(18.5%).The rSj31/32-VRS level of IgG rose at week 3,reached the peak at week 4 and then g radually dropped off.ConclusionAntibo dy might play an important role in the protective immunity elicited by rSj31/32.
Histochemical Observation of SDH, AKP and ACP in Cultured Cells from Schistosoma Japonicum
LIU Qing, DONG Hui-fen, JIANG Ming-shen
2002, 18(5): 557-558. doi: 10.11847/zgggws2002-18-05-27
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ObjectiveTo study histochemical observation of the succinodehydrogenase(SDH),alkaline phosphatase (AKP)and acidic phosphatase(ACP)in the cultured cells from adult Schistosoma japonicum.MethodsActivities of the three enzymes in the cultured cells were studied.ResultsThe results showed that SDH,AKP and ACP in all cultured cells possessed activities.Activities of SDH were found strongest around the membrane of the larger cells and in the flagellum part of the flagellated cells.Activities of AKP in the round granular,deltaic fan-like and multinucleate subtegumental cells were weaker in cytoplasm and stronger in the nucleus,while those in the clustered polygonal cells being stronger in cytoplasm,and in the flagellated cells stronger in both nucleus and flagellum part.ACP was very rich in all cultured cells,particularly around the nucleus and in cytoplasm of the larger cultured cells.ConclusionHistochemical coloration for schitosoma japonicum, SDH,AKP and ACP in cultured cells might be an evaluating index of brief,facility and sensitivity on the condition of cultured cells.
Case-control Study on Risk Factors of NIDDM
LI Jian, CHEN Min, YE Jie
2002, 18(5): 559-560. doi: 10.11847/zgggws2002-18-05-28
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ObjectiveTo explore the risk factors of NIDDM.Methods100 NIDDM patients who corresponded to the standard of diagnosis were selected in seven hospitals of Nanchang city from Feburary to May in 2000,and NON-NIDDM patients as contrastin the same hospitials were selected with the ratio of 1B1 of same sex,difference within 5 years,similar residental districts,then a 1B1 matched case-control study was carried out.ResultsAfter being analysed by Logistic analysis of single factor,it showed that the NIDDM groups were evidently higher than the NON-NIDDM groups in family history,hyper tension,obesity,eating too plentiful fat,liking meats,liking eggs,liking vegetables,etc.Then Logistic regression analysis of many factors was conducted on basis of Logistic analysis of single factor.It showed the NIDDM groups were evidently hig her than NON-NIDDM groups in family history,hypertension,eating too plentiful fat.ConclusionFamily history,hyper tension,eating too plentiful fat were risk factors of NI DDM.Itindicated that prevention for NIDDM should execute sy nthetic methods and control all kinds of risk factors.
Correlation Investigation of Condom Used in High Risk Crowd
WANG Wu-xin, Liu Zong-gan, Li Qing
2002, 18(5): 561-562. doi: 10.11847/zgggws2002-18-05-29
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ObjectiveTo investigate the usage rate of condom in prostitution and tuck driver along highway in different times and places,and analysis the cause of this situation and the correlation with STD/AIDS.MethodsTo select the prostitution and long-distance truck drivers in correlate places along No.105 highway.To investigate their difficult-easy degree obtaining condom,and usage rate of condom in the time of latest sexual behavior before and after intervention,and make correlated analyses with the prevalence rate of STD/AIDS in last one year.ResultsCondom could be obtaineed easier in suburban area where colligation scores of driver group and female group were 6.7 and 7.4 respectively.But condom could be obtaineded more difficultly in remote area where colligation score of two groups were 6.5 and 3.6 respectively.One month before and after intervention,the usage rates of condom of high risk female were 39.4% and 52.5%,and of truck driver were 38.2% and 57.3% respectively.The difference before and after intervention is much obvious(χ2=12.68,P<0.01).Half year after intervention,usage rates of condom of female group and driver group were reduced to 43.9% and 46.9% respectively,which comparing with that before intervention had not obvious diffence(χ2=2.08,P>0.05).In last one year,prevalence rates of STDs in female group and driver group were 34.38% and 28.75% respectively.The diseases included mainly non-gonococcal urethritis,syphilis and gonorrhea.The correlation of STDs prevalence rate was obvious(r=0.992,P<0.01).Conclusion(1)Condom was obtaineded difficultly in remote area along highway so that usage rate of it was low and prevalence rate of STDs was high relatively in female group.The chance of obtaining condom was more in driver group than in high risk female group.(2)Health education intervention can improve usage rate of condom in short period buthas not obvious effect in long time.(3)The high prevalence rate of STDs in high risk crowd along highway is connection with lower usage rate of condom.
Study of Intentional Injury Behaviors and Its Social-psychological Characters Among Secondary School Students of Anhui Province
ZHANG Hong-bo, TAO Fang-biao, ZHENG Guang-yu
2002, 18(5): 563-565. doi: 10.11847/zgggws2002-18-05-30
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ObjectiveThe study examines prevalence rates and related social-psychological factors of intentional injury among secondary school students and documents preventive intervention survey.MethodsThe 12 430 students in grads 712 were surveyed using an anony mous questionnaire.ResultsThe prevalence rates of intentional injury rangen from 2.5% to 30.4% and are related to sexand grade.The results of multivariate Logistic regression analysis show that school record,education level of father,depression and anxiety are associated with suicidal plan.ConclusionPrevention of intentional injury behaviors should take into account related social factors and the mental disorder.
2002, 18(5): 565-565. doi: 10.11847/zgggws2002-18-05-31
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Effect of Lead on Hippocampi Tissues of Mature Rats During Time of Growth After Birth
LI Peng, ZHANG Xue-mei, DING Ying
2002, 18(5): 566-568. doi: 10.11847/zgggws2002-18-05-32
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ObjectiveTo observe the effect of lead on the expressions of gene of NGF of hippocampi tissues of mature rats during the time of growth after birth.MethodsTo have the mother rat assimilate 0.2% of lead acetate after the birth of the young,the youngrat assimilate lead thr ough the milk.And adopt the method of RT-PCR to semi-quanititatively analyse the change of the content of NGF mRNA in the hippocampi tissues of the youngrat after the contact with lead during time of growth after birth.ResultsCompared with the control group.The content of lead in the lead-po lluted youngrat the hippocampi tissue went up,while the content of NGF mRNA in the hippocampi tissue went down.ConclusionThe pollution of lead may reduce the quantity of the show of NGF mRNA in the hippocampi tissues of youngrat when they were in the time of growth after the birth.
2002, 18(5): 568-568. doi: 10.11847/zgggws2002-18-05-33
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Study on Nurobehavioral Abnormal of Offspring of Rats Exposed to Hyperthermia and Cigarette Smoking
FENG Hui-gen, LI Yan-lan, YANG Bao-sheng
2002, 18(5): 569-570. doi: 10.11847/zgggws2002-18-05-34
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ObjectiveTo study the effect of the hyperthmia and cigarette smoking on the neonate rat's neurobehavioral.MethodsRats pregnant for 10 days were exposed to high temperature(41℃ or 42℃).Water-soluble substances containing cigarette smoking(1.10 cigarette/20 g rat)were given through subcutaneous inject at 2,4,6,8,10,12,14 days of gestation.The neonate rat's neurobehavioral(the appearance of four physiological markers,the acquisition of four reflexes,learning and memory)was observed.ResultsIn contrast to the control group and 37℃ group,when pregnant rats were exposed to high temperature(41℃ and 42℃)the neonate rats were delayed for the appearance of four physiologic markers(eye opening、pinna detachment、hair growth and tooth growth),the age of acquisition of four reflexes(surface righting,air righting,virual placing and auditory orienting)and the time of learning and memory(P<0.01).There was significant combined effect between the sustained hyperthermia of longer time and cigarette smoking,and there was a significant difference compared with the neurobehavioral of those only in single hyperthermia(P<0.01).ConclusionThe sustained hyperthermia combined with cigarette smoking can increasely delayed the neurobehavior growth of neonate rats.
Effects of Iron Deficiency of Trace Elements on Offspring Female Rats
ZHENG Yan-yan, XIE Yun-feng, XIAO De-qiang
2002, 18(5): 571-572. doi: 10.11847/zgggws2002-18-05-35
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ObjectiveTo study the effects of iron deficiency anemia(IDA)on the offspr ing female rats development and to observe their changes of trace elements in serum and tissues.MethodsFour-week-old female Wistar rats were randomly divided into two groups,experimental group and control group were fed on low iron and normal diets respectively for 14 weeks,and then mated with normal adults male rats.The day on which the plugs were found was desig nated day 0 of pregnancy.Weanling female and tow-day-old and forty-day-old rats were killed and the trace element of Fe、Zn、Cu、Mn、Se in serum and tissues of liver、brain and spleen were measured.Results(1)The content of Fe、Cu、Mn in serum of IDA female rats were much lower than that of controls(P<0.01,P<0.05 respectively).The level of Fe、Cu in serum of IDA offspring rats markedly lower than of controls(P<0.01).(2)The trace elements content in tissues of liver、brain and spleen:1Fe in IDA female rats were lower than that of controls while Zn、Cu、Mn、Se were not significantly different.④Fe in tissues between IDA offspringr ats and controls were significantly different.Cu、Mn in liver tissues of two-day-old rats were higher than that of controls.ConclusionIt was suggested that IDA can not only cause the deficiency of Fe in female and offspringrats but also change other trace element metabolism.
Study on Levels of Serum TNF-α in Diet-Induced Obese Rats
SHI Wan-ying, YAO Xing-jia, DONG Dan-na
2002, 18(5): 573-574. doi: 10.11847/zgggws2002-18-05-36
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ObjectiveExploring the levels of serum tumor necrosis factor alpha(TNF-α)in diet-induced obese rats.MethodsThirty male Sprague-dawley rats were divided into control group and high-fat group randomly,and they were maintained respectively a standard diet and a high-fat diet for 14 weeks.When the study was over,obese rats were sieved according to their body weight gain,then the levels of serum TC,TG,HDL-C and TNF-αwere determined.ResultsThe levels of serum TG,TC,HDL-C and TNF-α in obese rats were 0.92±0.19mmol/L,2.04±0.27mmol/L,0.54± 0.08mmol/L,2.95±0.35ng/ml respectively,they were all higher than those from control rats(P<0.01),and the TNF-α levels correlated significantly with the TG levels(r=0.464,P=0.03),negatively correlated with the HDL-C levels(r=0.683,P=0.02).ConclusionTNF-α perhaps involves in the mechanism of obesity and related complications.
Study on Nutritional Value of Silkworm Pupa Powder
WANG Bao-gui, SUN Xiao-xia, ZHAO Lin-yi
2002, 18(5): 575-576. doi: 10.11847/zgggws2002-18-05-37
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ObjectiveTo explore the nutritional value of cordy ceps.MethodsTo determine the content of protein,moisture,fat,fibre and ash by adopting chemical assay,to assay the essential amino acid content of protein by using Hitachi 835-50 automatic amion-acid analyzer the male ablactation Wister rat were fed by the food according to AOAC(protein 10%) for 28d,azote metabolizing test was conducted in the last 4d.ResultsThe net digestibility,biological value,net utilization rate and efficacy odds of cordyceps have nonsignificance compared with the casein bundle and the bean flour bundle(P>0.05).ConclusionCordyceps is edible insets protein with abundant nutritional value.
2002, 18(5): 576-576. doi: 10.11847/zgggws2002-18-05-38
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Intial Study on Inhibital Effect of Bacillus Natto on O157:H7.Staphylococus Aureus and Salmonella
JIA Li-min, CHENG Xiao-wei, JIANG Xiao
2002, 18(5): 577-578. doi: 10.11847/zgggws2002-18-05-39
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ObjectiveTo study the change of bacteria number when Bacillus natto was cultured with O157:H7、Staphylococcus Aureus、Salmonella respectively.MethodsTo cultivate bacillus and three types of pathogenetic bacteria respectively and to purify meanwhile.①To select cultivated bacillus and to cultivate them with pathogenetic bacteria together;②To cultivate bacillus for 8 hr then add pathogenetic bacteria.To observe inhibital effects respectively.ResultsThe effect of Bacillus natto on Staphylococcus Aureus was apparent.Bacillus natto had inhibital effect on O157:H7 on some degree,while it didn't have apparent effect on Salmonella.ConclusionAfter Bacillus natto was cultured for 6~8 hours,aureus O157:H7,Staphylococcus Aureus was aureused.The effect of inhibition was more apparent.
Study on Application of McAb-ELISA to Filaria Surveillance by Detecting Filaria-specSifie IgG4
CHEN Xi-xin, DENG Xu-li, LI Gui-ping
2002, 18(5): 579-580. doi: 10.11847/zgggws2002-18-05-40
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ObjectiveTo discuss the practical value of McAb-ELISA to detect filaria speicfic IgG 4.MethodsSeven strains of filaria-specific McAb were established through technique of cellular fusion and biochemistry-immunolo gy,and were used in filarial infection test as probes to detect filaria-specific IgG4.ResultsIts sensitiv ity was 96.3% (104/108).It detected as low as 0.01μl/ml IgG4 per milliliter serum.No cross-action were tested with serums collected from residents of non-endemic area,including those infected with intest inal nematodes,liver fluke and systicercus.Its specificity was 100%.It was also feasible and practical when applied to field study,effectively reflected the epidemic situation of local filarial infection.ConclusionThis was an ideal method to be used in filarial infection surveillance.
2002, 18(5): 580-581. doi: 10.11847/zgggws2002-18-05-41
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Development of Portable Formaldehyde Meter of High Sensitivity Using for in Situ Determination of Formaldehyde in Air
CHEN Huan-wen, GUO Ji-guang, YU Ai-min
2002, 18(5): 582-583. doi: 10.11847/zgggws2002-18-05-42
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ObjectiveTo etablish a method characterized by high analytical speed,less cost,much more convenience,good accuracy and selectivity.MethodsA new portable formaldehyde meter and special reagent packages for determining for maldehyde in air in situ was developed.ResultsThe linear range was 0.001-0.3mg/L and the determination limit was 0.001mg/L.The RE of this method for determining formaldehyde in air was no more than 5%.ConclusionAll results of different practical samples were satisfactory though the analytical pro cedure was extremely simple.
2002, 18(5): 584-584. doi: 10.11847/zgggws2002-18-05-43
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Analysis on People's Health Status in Hangzhou
JIN Da-feng, MAO Yi-ping, FANG Sun-yuan
2002, 18(5): 585-587. doi: 10.11847/zgggws2002-18-05-44
Abstract(1207)
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ObjectiveTo know the peoples health status in Hangzhou,and to find out the factors influencing health status.To afford the basis to make the disease control policy.MethodsTo analyze people's health status and risk factors comprehensively by mortality rate、YPLL rate(years of potential lost rate)、WYPLL rate(working years of potential lost rate)and VYPLL rate(valued years of potential lost rate).ResultsThe mortality rate of the city was between 4.66 and 5.66, while it was betw een 6.57 and 7.14 in the country from 1995 to 2000.There were no much differences in every year.The YPLL rate descends both in the city and in the country.In the city,it was from 32 13 down to 24.09.In the country,it was from 50.06 down to 42.31.The reasons causing people death were injury poisoning、malig nance and car diovascular disease.According to the analysis on the VYPLL(valued years of potential lost),they were injury poisoning、congenial malformations、psychoses and diseases of the nervous system.ConclusionThe people's health status in Hang zhou had been improved.The main reasons causing people death were injury poisoning、malignance、cardiovascular disease、cong enial malformations、psychoses and diseases of the nervous system.
2002, 18(5): 588-590. doi: 10.11847/zgggws2002-18-05-45
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Analysis and Prediction on Lung Cancer Mortality Rate Trend of Xuanwu District, Nanjing City
ZHAO Li, ZHANG Zhi-liang, CHEN Yi-lin
2002, 18(5): 591-592. doi: 10.11847/zgggws2002-18-05-46
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ObjectiveTo investigate the changing trend of lung cancer mortality rate in Nanjing city.MethodsGroup samplings were carried out among the resident of Xuanwu District,Nanjing City,the residents death data since 1970 was analysised.The recent lung cancer mortality rats was predicted by gray model.ResultsThe lung cancer mrotality rate for Xuanwu District,Nanjing City was increased continuously.Lung cancer was in the second place among all malignancy death factors in the 1970s and 1980s,but leaps to the first place since 1990 and accounts for 23.45% among all malignancy death factors between 1997 and 1999.The mortality rate and increasing speed of lung cancer for men was higher than that of wome.It was predicted by gray model prediction that the recent lung cancer mor tality rate for men and wome would reach 47.39%/105 and 22.63/105 respectively until 2000-2002;51.24/105 and 24.27/105 respectively until 2003-2005.Conclusions The lung cancer mortality rate has been raised to the first malignancy death factor in Nanjing.In the coming five years,lung cancer would still have arising trend if no effective measures were adopted.
2002, 18(5): 593-593. doi: 10.11847/zgggws2002-18-05-47
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2002, 18(5): 594-594. doi: 10.11847/zgggws2002-18-05-48
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2002, 18(5): 595-595. doi: 10.11847/zgggws2002-18-05-49
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2002, 18(5): 596-596. doi: 10.11847/zgggws2002-18-05-50
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Study of Hepatitic Virus Infection in Patients on Hemodialysis
YU Xiao-nan, JIANG Li, FU Wei-xin
2002, 18(5): 597-598. doi: 10.11847/zgggws2002-18-05-51
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ObjectiveTo study the infect ion of hepatitis Bvirus(HBV)、hepatitis Cvirus(HCV)and hepatitis G virus on hemodialysis.MethodsHBsAg,HBsAb,HBeAg,HBeAb,HBcAb,and anti-HCV and anti-HGV were measured in 1 478 patients on hemdialysis over 3 mothes by Enzyme Immunassay.HBV-DNA was determined by polymerase chain reaction (PCR).100positive patients of them for anti-HCV and anti-HGV were determined for HCV-RNA and HGV-RNA by rev erse transcription+nested poly merase chain reaction(RT-PCR).ResultsIn 1 478 patients,the infection rate of HBV was 43.9% (649/1 478),7.9% (117/1 478)patients were positive for HBV-DNA,the infection rate of HCV was 42.6% (630/1 478);the infection rate of HGVwas 46.4% (685/1 478);In 100 positive patients of anti-HCV and anti-HGV, 73% and 77% patients were positive for HCV-RNA and HGV-RNA.ConclusionHepatitis virus infection exists in patients on hemodialysis.It was very important to study risk factors that were related to hepatitis virus.
2002, 18(5): 598-599. doi: 10.11847/zgggws2002-18-05-52
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2002, 18(5): 600-600. doi: 10.11847/zgggws2002-18-05-53
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2002, 18(5): 601-602. doi: 10.11847/zgggws2002-18-05-54
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2002, 18(5): 603-603. doi: 10.11847/zgggws2002-18-05-55
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2002, 18(5): 604-605. doi: 10.11847/zgggws2002-18-05-56
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2002, 18(5): 605-605. doi: 10.11847/zgggws2002-18-05-57
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2002, 18(5): 606-606. doi: 10.11847/zgggws2002-18-05-58
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2002, 18(5): 607-607. doi: 10.11847/zgggws2002-18-05-59
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2002, 18(5): 608-609. doi: 10.11847/zgggws2002-18-05-60
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2002, 18(5): 609-609. doi: 10.11847/zgggws2002-18-05-61
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2002, 18(5): 610-610. doi: 10.11847/zgggws2002-18-05-62
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2002, 18(5): 611-611. doi: 10.11847/zgggws2002-18-05-63
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2002, 18(5): 612-612. doi: 10.11847/zgggws2002-18-05-64
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2002, 18(5): 613-613. doi: 10.11847/zgggws2002-18-05-65
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2002, 18(5): 614-615. doi: 10.11847/zgggws2002-18-05-66
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2002, 18(5): 615-616. doi: 10.11847/zgggws2002-18-05-67
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2002, 18(5): 617-618. doi: 10.11847/zgggws2002-18-05-68
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Acknowledge and Attitude to AIDS and Educational Efficacy Evaluation in College Students
HUANG Wan-qi
2002, 18(5): 619-620. doi: 10.11847/zgggws2002-18-05-69
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ObjectiveTo disseminate the acknow ledge about AIDS in young people and to strength their ability of selfprotection and prevention to the AIDS.MethodsBy random group sampling 1 299 students completed the baseline survey about acknowledge and attitude to AIDS,then accepted the intervention by health education about AIDS and efficacy evaluation to it.ResultsBy inter vention of health education,the students.correctrate answering to acknowledge about AIDS significantly increased 3.6% to 54.3% (P<0.01).The intervention indexes are all above 1.ConclusionIt would be very efficatious to disseminate the acknowledge about AIDS in students by health education in colleges.
2002, 18(5): 621-622. doi: 10.11847/zgggws2002-18-05-70
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Field Trial of Screen on Combination of Attractants of Rodenticide Baits by Orthogonal Design
JIA De-sheng, TIAN Shu-lin, LU Nian-hong
2002, 18(5): 623-624. doi: 10.11847/zgggws2002-18-05-71
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ObjectiveThe orthogonal design was applied to the choice feeding test on field to screen on the combination of attractants of rodenticide baits and to evaluate the palatability of the trial poison baits and the mortality of the most suitable trail poison bait.MethodsThe orthogonal table L18(21×37) was selected.18 kinds of 0.005% bromadiolone trial poison baits combined by glucide,peanut oil,salt and alcohol on 2~3 levels of concentration and the control poison bait were tested in the choice trial on field,in which SPLM was implemented in analysis of variance.ResultsThe 13th trial poison bait was the most suitable,which feeding coefficient was 1.55,relatively that of control poison bait was 0.51.On the field poisoning test,the most suitable poison bait gave 100% mortality,but control poison bait 79.0%.ConclusionThe field trial of screen on the combination of attractants of rodenticide baits by the orthogonal design was feasible;the mortality of the most suitable trail poison bait was better.
Grey Series GM(1, 1)Model in Prediction of Cardiocerebrovascular Disease
ZHOU Mai-geng, YANG Gong-huan
2002, 18(5): 625-627. doi: 10.11847/zgggws2002-18-05-72
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ObjectiveTo give a comprehensive description of GM(1,1)modeling for prediction of Cardiocerebrovascular diseaes.MethodsAn example of predicting the rate of Cardiocerebrovascular disease is employed to describe the technique.ResultsThe nationwide mortality of Cardiocerebrovascular disease tends to increase year by year,among it,the mortality of cerebrovascular disease and Isechemic heart disease increase more obviously.ConclusionGM(1,1)model can be well used to the short-term prediction of Cardiocerebrovascular disease,but it is not very well in midterm or long-term prediction.
2002, 18(5): 628-629. doi: 10.11847/zgggws2002-18-05-73
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2002, 18(5): 629-629. doi: 10.11847/zgggws2002-18-05-74
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2002, 18(5): 630-631. doi: 10.11847/zgggws2002-18-05-75
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2002, 18(5): 632-634. doi: 10.11847/zgggws2002-18-05-76
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2002, 18(5): 634-635. doi: 10.11847/zgggws2002-18-05-77
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2002, 18(5): 636-636. doi: 10.11847/zgggws2002-18-05-78
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2002, 18(5): 637-638. doi: 10.11847/zgggws2002-18-05-79
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2002, 18(5): 638-638. doi: 10.11847/zgggws2002-18-05-80
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2002, 18(5): 639-639. doi: 10.11847/zgggws2002-18-05-81
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2002, 18(5): 640-640. doi: 10.11847/zgggws2002-18-05-82
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