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2003 Vol. 19, No. 9

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Study on psychological behaviors of people in 5 cities of North China under SARS situation
SHI Kan, LI Wen-dong, LE Guo-an,
2003, 19(9): 1025-1027.
Abstract(1348) HTML (291) PDF 263KB(80)
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  Objective   Exploring people's characteristics of risk perception under SARS situation, finding out how risk perception affects behavior and mental health, offering helpful research result for the related governmental department and for the public, offering psychological evidence and coping strategies.   Methods   By stratified sampling and convenient sampling, survey of 1500 people is taken in Beijing, Tianjin, Shijiazhuang and Huhehaote.People's risk perception, degree of tension, prediction about when SARA would be controlled, mental health, coping behavior and prediction about the economic development are explored by Likert Scale and GHQ.   Results   (1) SARS information from nearby place can draw more attention, urban people in Beijing were more sensitive to infection and recovery information than those in nearby cities.(2)Female fell more risk, government and public information are drawn more attention by middle and elder people, youth under 20 pay more attention to information related to their study and life, and people above 50 were more sensitive to recovery information than other groups.(3)Risk assessment of people near Beijing were significantly lower than that of people in Beijing, and college student's mental health should be paid more attention in some place.   Conclusion   It can be understood that the difference of people's risk perception and its effect on different coping behavior and mental health of different groups by taking survey of people in emergency.The research of people's social-psychological behavior should be improved in public emergency.
Analysis on effect of air pressure and air temperature on outbreak and epidemic of SARS
YANG Zhi-cong, DU Ling, WANG Ming,
2003, 19(9): 1028-1030.
Abstract(3042) HTML (1539) PDF 627KB(94)
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  Objective   To analyze the relationship between incidence of SARS and air pressure and air temperature, to explore nature and environment factors that affect SARS outbreak.   Methods   To adopt descriptive epidemiology, correlation and regression statistics to study the relation of correlation and multiple linear regression between incidence of SARS in Guangzhou and Hong Kong and local average air pressure and average air temperature.   Results   Average air pressure is 5.5, 6.2 hectopascals respectively compared with breakout period and subsidizing period of SARS in Guangzhou, and Hong Kong.Average air temperature are under 4.3℃ and 6.0℃ respectively, incidence of SARS has positive correlation with average air pressure, negative correlation with average air temperature.Multiple linear regression indicates the sigificant difference between incidence of SARS and temperature and pressure.   Conclusion   Outbreak and epidemic of SARS is affected by air pressure and air temperature.
2003, 19(9): 1030-1030.
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Tactic and its effect on prevention and treatment of SARS in Shenzhen city
CHENG Jun-quan, ZHOU Jin-an, ZHOU Li
2003, 19(9): 1031-1032.
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  Objective   To summarize the effect and the experiences during preventing and treating SARS in Shenzhen city by analyzing the SARS epidemic situation, the emergent tactic and its effect on SARS prevention and treatment.   Methods   The methods of epidemiology and social medicine were applied to collect, sort and analyze the information about SARS epidemic situation, emergency measures and results.   Results   Both incidence rate(0.76/100 000)and mortality rate(1.8%) were lower, meanwhile the cure rate(86.9%)was high and no medical staff were infected.The experiences such as judging scientifically, making decision decidedly, screening effective preventive measures, medical staffs taking preventive drugs, health promotion and citizen's physical exercise etc, were the guarantee to acquire the victory against SARS.   Conclusion   The effect of controlling SARS was remarkable in Shenzhen, and these measures provided valuable experiences in SARS prevention and treatment for the other cities in the future.
2003, 19(9): 1032-1032.
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2003, 19(9): 1033-1033.
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2003, 19(9): 1034-1034.
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2003, 19(9): 1035-1035.
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2003, 19(9): 1036-1036.
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2003, 19(9): 1036-1037.
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2003, 19(9): 1037-1037.
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Study on time and geography distribution of reproduction-endocrine related tumors in China
LI Jia-yuan, LUAN Rong-sheng, WU De-sheng,
2003, 19(9): 1038-1040.
Abstract(966) HTML (257) PDF 391KB(47)
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  Objective   To analyze time trend and area distribution of mortalities of female breast cancer, ovaries tumors, prostate tumor and testicular cancer in China.   Methods   Surveillance mortality data of breast cancer, female ovaries tumor, prostate tumor and testicular cancer from 1991 to 1999 on 145 surveillance spots were collected through Chinese disease surveillance system.Grey model(GM(1, 1))was used to analyze time trend of these four cancers.Data of total grain output and total quantsity of sewage water, exhaust gas and solid waste discharged from industrial production of all the 31 provinces of China in 1999 were collected.K-means cluster analysis was used to classify the provinces.ANOVA was used to analyze different mortalities between the classified areas.   Results   The surveillance rate of br east cancer in China incr eased according to surveillance years, and the GM(1, 1)was ŷ(t+1)=(1.529+47.29)e0.0274T-47.29.In those areas where industrial pollution was serious, mortalities of breast cancer were significant higher than other areas.In those areas where grain output was highor industrial pollution was serious, mortalities of breast cancer showed an increasing trend along with years.   Conclusion   Breast cancer may be related to industry and agriculture pollution in our country, and environmental endocrine disrupting chemicals may play an imporant role in breast cancer.
Expression of fetal hemoglobin gene in maternal peripheral blood
WANG Bin-you, XU Tan, CHEN Fei,
2003, 19(9): 1041-1043.
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  Objective   To explore the method for fetal hemoglobin gene expression from peripheral blood of pregnant women.   Methods   mRNA extracted from peripheral blood of pregnant women was reversed transcript.ε and γ gene was used as a primer to amplify fetal DNA.PCR reactant were analyzed on agarose gels and observed under gel works system.   Results   44 samples were amplified with fetal ε and γ gene and 6 with negative in 50 samples.   Conclusion   RT-PCR methods can be used to amplify ε and γ gene directly from peripheral blood of pregnant women.Little amount of fetal mRNA from maternal blood can be nearly enough for reverse transcription and amplication.Non invasive diagnosis for fetal diseases may be possibility.
2003, 19(9): 1043-1044.
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Effects of vitamin A deficiency on expression of Hox-3.05 mRNA in mouse embryos
LIU Lie-gang, LIU Gong-ping, FAN Jian-she,
2003, 19(9): 1045-1046.
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  Objective   To investigate the effect of vitamin A(VA)deficiency on Hox-3.5 mRNA expression in mouse embryos.   Methods   Sixty Kunming female mice were divided into four groups: normal control group N〔4 000 UVA/(kg°diet)〕, VA deficiency group A[0IUVA/(kg°diet)]mVA-supplement after 0 d.p.c(day post coitus), group B and VA-supplement after 7 d.p.c group C.〔B、C were fed 0IU VA/(kg°diet)〕before crossover, B was fed 10 000 IUVA/(kg°diet) after 0 d.p.c and C was fed 〔10 000 IU VA/(kg°diet)〕after 7d.p.c..On the 12th day p.c.pregnant mice in every group were killed and the uterus were cut open to take out the mouse embryos immediately.Mean while, blood was kept from pregnant mice for the determination of serum VA.In situ hybridization was used to deter mine Hox-3.5 mRNA expression in the mouse embryos by digxigenin-labeled cDNA probes.   Results   Hox-3.5 mRNA expression in the embryos of group A and C decreased significantly compared with group N and B(P < 0.01 orP < 0.05), and there was no difference between group B and group N.The contents in group C multiplied compared with group A, but still reduced compared with group N.   Conclusion   VA may regulate Hox gene expression on transcriptal level.
2003, 19(9): 1047-1048.
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Analysis on death cause of residents in flood disaster areas in Dongting lake, Hunan province
LI Xing-li, LI Shuo-qi, TAN Hong-zhuan,
2003, 19(9): 1048-1049.
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  Objective   In order to find out the effects of flood disaster on the death level and proportional mortality rate.   Methods   Through stratified sampling, some areas suffered from flood disastr during 1995-1999 and the control areas that not suffered from flood from 90.s were chosen.   Results   The mortality rate in flood disaster areas was higher than that of the control(685.33×10 -5 vs 609.99×10 -5).The order of death cause was same in the two residents, the respiratory and circulatory system diseases were the main death cause.The mortality rates of injury, poison and malignant tumors in the flood disaster areas were higher than that in non-flood areas too.   Conclusion   The flood disaster resulted in the increase of mortality.Intervention measures on decrease should beenforced.
Lipid peroxidation injuries of hepatic subcellular fractions in mice caused by chronic fluorsis, arsenism poisoning
LUI Qi-zhan, HUANG Hou-jin, DONG guo-bin,
2003, 19(9): 1050-1051.
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  Objective   To study the levels, similarities and differences of the lipid peroxidation injury effects in hepatic subcellular fractions caused by chronic fluorosis, arsenism and fluoride-arsenic poisoning respectively in mice.   Methods   120 mice were randomly divided into four groups which were fed with four different diets supplemented respectively fluoride arsenic and fluoride-arsenic for 6 and 12 months.The LPO contents in hepatic mitochondria and microsomes and the activities of SDHase in hepatic mitochondria and G-6-Pase in hepatic microsomes were determined.   Results   In the three poisoning groups: the LPO contents in hepatic mitochondria and microsomes were significally increased.The activities of SDHase in hepatic mitochondria and G-6-Pase in hepatic microsomes were markedly decreased.The increases of LPO contents in two subcellular fractions showed significally negative correlation to the decreases of their enzymes activities, respectively.However, the effect of the three diffierent poisons on these indices were different.   Conclusion   In mice, the lipid peroxidation injuries in hepatic mitochondria and microsomes were caused by the chronic three poisons, to some extent, fluoride and arsenic can influence the effect together.
Study on protective effect of lycium barbarum polysaccharides on testis tissue damage of male rats
HUANG Xiao-lan, YANG Ming-liang, WU Xiao-min,
2003, 19(9): 1052-1054.
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  Objective   To study the protective effect of lycium barbarum polysaccharides(LBP)on male rats' testis organ damage and find out the optimal dose of LBP.   Methods   Different doses of LBP [0, 10, 50, 100 and 200 mg/(kg°d)] were poured into the male Wistar rats' stomach.Trauma model of testicle were constructed by warm bath after two weeks.Serum sexual hormone levels, organ index of testis and epididymides, superoxide dismutase(SOD)activity, malonic aldehyde (MDA)contents and morphological change of the testis were observed.   Results   LBP could elevate the serum sexual hormone levels and organ index of testis and epididymides, increase the activity of SOD and decrease the contents of MDA in testis tissue; and make the damaged testis recover to near normal.   Conclusion   LBP has the protective effect on damaged rat testis and the optimal dose is 10 mg/(kg°d).
Effects of cisplatin and sodium selenite on cell proliferation kinetics of human T lymphocytes
CHEN Xian-jun, ZHAO Hong-gang
2003, 19(9): 1054-1056.
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  Objective   To explore the effects of cisplatin and sodium selenite on cell proliferation kinetics of human T lymphocytes and the possible prevention of sodium selenite against the cell proliferation inhibition by cisplatin.   Methods   Human peripheral lymphocyte cultures were treated with three concentrations(0.05, 0.20 and 0.50 mg/L)of cisplatin alone or in combination with sodium selenite during the last 48 h of incubation.Sodium selenite was with a single dose of 0.05 mg/L at the beginning of the cell culture or at the same time as cisplatin was administered for different treatments.Cell cycle profiles and cell cycle time of cell populations treated with different ways were detected by differential staining of newly synthesized DNA and chromatids that had incorporated 5-bromodeoxyuridine during the cell culture.   Results   Cisplatin at the three doses notably arrested cell cycle processes and remarkably lengthen cell cycle time.Sodium selenite at 0.05 mg/L presented the effects of promoting cell cycle progresses and shortening cell cycle time.When cells were cotreated with cisplatin and sodium selenite, and when cisplatin concentration was 0.05 mg/L, sodium selenite in pretreatment and simultaneous treatment markedly resisted the actions that cisplatin arrested cell cycle processes and lengthened cell cycle time; when cisplatin concentration was 0.20 mg/L, sodium selenite in pretreatment could antagonize the actions of cisplatin on cell proliferation kinetics but sodium selenite in simultaneous treatment could not; when the concentration was 0.50 mg/L, sodium selenite did not present the above effects.   Conclusion   Sodium selenite in concentration of 0.05 mg/L can facilitate cell cycle processes, shorten cell cycle time of human T lymphocytes in culture, and can antagonize the influence of lower dose cisplatin on cell proliferation kinetics.Pretreatment with sodium selenite can obtain more efficient effects.
Expression changes of connexin 32 in induced forestomach neoplasia of mice
LI Bai-xiang, ZHANG Xiao-feng, MA Ruo-bo
2003, 19(9): 1057-1058.
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  Objective   To investigate the dynamic expression changes of connexin 32 in the precancerous lesions and forestomach neoplasia.   Methods   B(a)P was used to induce the forestomach neoplasia of the mice, and immunohistochemistry technique was used to detect the expression regularity of connexin 32 in the procedure of inducing forestomach neoplasia.   Results   The expression of connexin 32 manifested the gradient changes from high level to low level, and finally deletion.That is to say, expression of connexin 32 was highly level in the normal mucosa, obviously decreased or disappeared in the atypical hyperplasia mucosa, and had no expression in the cancer tissue.   Conclusion   The abnormal expression of connexin 32 gene had a close relationship with the stomach cancer.The reductive or deletive expression of connexin 32 caused the disorder of gap junctional intercellular communication in the mucosa, de-differentiation and malignant proliferation of cells, ultimately developed forestomach neoplasia.
Effects of soybean ethanol etract on osteoblastic cell proliferation and TGFβ1
LI Wan-li, TIAN Yu-hui, SHEN Guan-xin,
2003, 19(9): 1059-1060.
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  Objective   To explore the effects of flavonoid of celosia cristata on proliferation, differentiation and excretion of TGFβ1 in vitro cultured osteoblasts.   Methods   Osteoblastic cells from newborn rat calvarial was cultured.Osteoblastic cells proliferation was measured by MTT, activity of ALP was observed.SP immunohistochemistry was used to measure the effects of flavonoid of celosia cristata on expression of TGFβ1 in vitro cultured osteoblasts.   Results   The flavonoid of celosia cristata had the effects of stimulating the proliferation, differentiation of osteoblasts and increasing the expression of TGFβ1 in vitro cultured osteoblasts in rat.   Conclusion   The flavonoid of celosia cristata can stimulate the proliferation, differentiation and the expression of TGFβ1 in calvarial osteoblastic cells.
Case-control study of conditional logistic regression on risk factors of perinatals' birth defects
QIN Dan-dan, QIU Xiao-qiang, ZENG Xiao-yun,
2003, 19(9): 1061-1062.
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  Objective   To explore the risk fctors for the occurrence of the perinatals birth defects in Guangxi province and plan some intervention measures.   Methods   Using conditional logistic regression to analyse environmental risk factors which 276 pairs perinatals'(pairmatching case-control)parents were exposed to in Guangxi province in the year 2000.   Results   Statistically significant(α< 0.05)risk factors were: maternal pesticide exposure during pregnancy(OR=5.764), maternal malnutrition during pregnancy(OR=4.595), paternal chemicals exposure(OR=2.505), maternal chemicals during pregnancy(OR=2.331), meternal parity more than twice(OR=1.934), maternal overloaded physical labor during pregnancy(OR=1.480), maternal unhealthy feelings during pregnancy(OR=1.460), which seemed relevant to the occurrence of the birth defects.   Conclusion   Birth defects can be intervened in the respects such as vocational factors, nutritional factors and psychological factors in Guangxi.
Study on malignant transformation of BALB/c3T3 cells induced by octachlorodipropyl ether
DONGg Ju, TANG Meng
2003, 19(9): 1063-1064.
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  Objective   To explore the effects of cell malignant transformation of octachlorodipropyl ether(S2).   Methods   Using BALB/c3T3 cells to do cells toxicity experiment to determine the scope of doses, then to do cells malignant transformation experiment and to verify the malignant character of transformational cells.   Results   S2 could induce the malignant transformation of BALB/cB T3 cells, and dose-dependent effect was noticed.   Conclusion   S2 possessed BALB/c3T3 cells malignant transformation ability and it was necessary to study it further.
Analysis on latent health demands and influential factors of medical service in rural area
CHEN Hua, CHEN Guan-min, SUN Jing
2003, 19(9): 1065-1066.
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  Objective   To identify the latent demands and influential factors of medical service in rural residents.   Methods   Using stratified-cluster random sampling method, 1839 individuals are interviewed in 5 counties in Yichang rural area.   Results   The average rate of non-visiting is 13.21%, male of which is 12.01% and female is 15.56%.Through logistic regression analysis, the influencing factors on the non-visiting rate are obtained.They are gender, health status, recognition for health, marriage status, different counties, and so on.   Conclusion   The cooperative medical system should be improvd in rural areas.It is very important for medical facilities to enhance the service quality, reduce the cost, keep the high medical fee in limits in order to provide more medical help and satisfy the health demand.
2003, 19(9): 1066-1066.
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Effect of vitamin A deficiency on iron nutritional status in rats
ZHAO Dan, WANG Chao-xu, SUN Chang-hao,
2003, 19(9): 1067-1068.
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  Objective   To study the effect of vitamin A(VA)deficiency on iron nutritional status in rats and explore the possible mechanism.   Methods   Forty male Wistar rats were divided randomly into four groups according to their body weights.They were Ⅰ VA and iron deificient group, Ⅱ entire VA deficient group, Ⅲ slight VA deficient group and Ⅳ normal VA group.After they were raised for eight weeks, the rats were sacrificed and the serum VA, serum iron, serum ferritin, hemoglobin and total iron in the liver were determined.The level of liver transferrin mRNA expression were measured using reverse transcription-polymerase chain reaction(RT-PCR)method.   Results   VA deficiency decreased serum ferritin, hemoglobin and total iron in the liver and inhibited the expression of transferrin mRNA in the liver significantly.   Conclusion   VA deficiency may inhibit the absorption and transportation of iron by reducing the level of transferrin mRNA expression in the liver.
Preparation of conjugate antigen and antibody to parathion
GUO Jian-ping, LIU Yun-xia, GAO Zeng-lin,
2003, 19(9): 1069-1071.
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  Objective   To produce the organophate parathion conjugate antigen antibody.   Methods   The hapten, which reduces the parathion changing-NO2 into-NH2, is conjugated diazo-linked with the carrier protein BSA to prepare conjugate antigen.By immunizating the New Zealand rabbit, the antisera is obtained.   Results   The result by NMR is δ: 6.9-7 (m, 2H, CH2)6.4-6.5(m, 2H, CH2)4.1-4.2(q, 4H, -CH2)3.4(s, 2H, NH2)1.3-1.4(t, 6H, CH3).The ratio of hapten to BSA is from 35-36 to 1.Test of the antisera by indirected ELISA and immunodiffusion shows that the titer is high and antibody is specific.The titer is 1:128 000.Precipitin bands are observed from the reaction of the antisera with the conjugate by immunodiffusion.The minimum concentration of ELISA inhibition for antisera is 0.16 ng/ml.There is no cross-reactivity between the antisera and serum analogues.The research confirms that the antisera is specific to the parathion.   Conclusion   The antigen is in effect.The research provides the preparation for producing McAb of parathion and sets up the ELISA method of detecting parathion.
2003, 19(9): 1071-1071.
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Observation on efficacy of whole cell DTP-HBV combined vaccine
ZHANG Zhe-min, HOU Qi-ming, MA Xiao,
2003, 19(9): 1072-1074.
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  Objective   To investigate the safety, efficacy and immunization schedule of whole cell DTP combined with hepatitis B vaccine(CHO)produced by Wu Han National Biological Institute.   Methods   Two phases were included in this trial.Three groups were included in the first phase.Group 1(30)was immunized with wDTP-HBV(CHO)according to 2, 4, 6 month schedule; group 2(30)was immunized wD TP-HBV(CHO)according to 3, 4, 5 month; group 3(31)was immunized wDTP vaccine according to 3, 4, 5 month.Phase 2 was also divided into 3 groups.Group 1(113)was immunized with wDTP-HBV according to 2, 4, 6 schedule; Group 2(113)was immunized with wDTP control vaccine in upper left arm, and HBV control vaccine on the upper right arm; Group 3(101)immunized with control HBV vaccine according to 0, 1, 6 month and DTP control vaccine according to 3, 4, 5 month.   Results   The body temperature of 1 child in group 1 and group 2 increased weakly, the rate was 3.3% among the whole group in phase 1 trial.In phase 2 trial, there were 4 and 5 children had mild side reaction in group 1(3.1%), 2(4.2%)respectively; 14 children had weak side reaction in group 3, the rate was 12.2%.No serious side reaction was found in phase 1 and 2.The antibody titer of all samples collected before the first dose and after the last dose were measured, the result showed that the sero-conversion of anti-diphtheria, anti-tetanus and anti-HBV antibody were 100%, > 85% for anti-pertusis antibody.The statistics analysis showed that all the antibody level of the samples before and after immunization had significant difference.The comparison of the antibody level among 3 groups showed that the new wDTP-HBV vaccine had good ability to induce effective antibody.   Conclusion   This study result certified that the new adsorbed whole cell DTP and HBV(CHO)combined vaccine had good safety and efficacy, and can be used for the immunization.The new 2, 4, 6 immunization schedule was also able to induce enough antibody level for diphtheria, pertusis, tetanus and hepatitis B virus.
2003, 19(9): 1074-1074.
Abstract(733) HTML (168) PDF 112KB(35)
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Effect sodium fluoride on proliferation of nephrocyte in rats in vitro
JIA Li-hong, LIU Li, YANG Jun,
2003, 19(9): 1075-1076.
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  Objective   To study the effect of NaF on proliferation of nephrocyte and protective efficiency of VE, Vc, Se and GSH.   Methods   Adding different concentrations of NaF, VE(25μmol/L), Vc(50μmol/L), Se(5μmol/L)and GSH (1mmol/L)into cultured nephrocyte using ELISA to determine the level of OD, and computing the proliferation rate of nephrocyte.   Results   NaF has two-side action on the proliferation of nephrocyte.   Conclusion   VE, Vc and GSH can antagonize the toxic effect of NaF(1 mmol/L)on nephrocyte.
Effects of soman intoxication under high altitude hypoxia on heart cholinergic system of rat
LIU Hui, LIU Yong, WANG Shi-li,
2003, 19(9): 1077-1078.
Abstract(822) HTML (260) PDF 402KB(29)
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  Objective   To study the effects of soman intoxication under high altitude hypoxia on the activity of AchE and the expression of m2 receptor mRNA.   Methods   Experimental Wistar rats were randomly divided into four groups: normal control group, high altitude hypoxia group, plain soman intoxication group and soman intoxication combined hypoxia group.The activity of AchE and the expression of m2 receptor mRNA in rats myocardium were measured with DTNB method and RT-PCR.   Results   Hypoxia increased the activity of AchE in different degrees, and the expression of m2 receptor also increased; during soman intoxication under high altitude hypoxia, the expression of m2 receptor mRNA enhanced remarkably compared with plain soman intoxication group.   Conclusion   Both high altitude hypoxia group and soman intoxication significantly interfered with the functions of the cholinergic nervous system in the heart.The up-regulation of cholinergic system may play an important role in the toxicity increasing of organophosphate compounds at high altitude.
Immune response induced by herpes simplex virus-2 DNA vaccine in mice
HONG Yan, YANG Lian-hua, CHEN Yong,
2003, 19(9): 1079-1080.
Abstract(986) HTML (404) PDF 764KB(27)
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  Objective   To evaluate the protective efficacy of DNA vaccine expressing herpes simplex type 2(HSV-2) full-length glycoprotein D(gD).   Methods   The whole encoding sequence of gD was amplified from HSV-2 genome by PCR, and then cloned into downstream of PCMV of pcDNA3, and resulted in the HSV-2 DNA vaccince-pcDNA3-gD.pcDNA3-gD was inoculated to mice by bilateral intramuscular injection into the rear legs.   Results   The mice inoculated with pcDNA3-gD could produce specific antibodies and survive after the lethal challenge with HSV-2.The rate of survival rate was 75%.   Conclusion   pcDNA3-gD can induce specific immune response and may be a candidate DNA vaccine D.
2003, 19(9): 1080-1080.
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Study on effects of zinc on γ-ray induced irradiation damage of bone marrow hematopoietic cells in mice
XIAO Yuan-mei, ZENG Ling-fu, WANG Zhou
2003, 19(9): 1081-1083.
Abstract(914) HTML (220) PDF 259KB(37)
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  Objective   To study the effects and possible mechanisms of zinc on the C-ray induced irradiation damage of the bone marrow(BM)hematopoietic cells in mice.   Methods   The BM cells(BMC)were separated from mice and were suspended in RPMI 1640 mediums.Zinc was added to the mediums, whose final concentrations in the mediums were 20, 200, 400, 800μmol/L respectively.Then the cells were irradiated by 60Coγ-ray, the dose was 5Gy.16 hours later, the number of BM karyote and the contents of DNA of BMC were measured.By flow cytometry(FCM)and immunihistochemistry technique, the rates of cell apopotosis, the changes of the cell cycle and the expression of genes were examined respectively.   Results   After irradiation, the number of BM karyote and the contents of DNA were decreased; the rates of cell apoptosis were increased.BM cells were blocked in Sphase, lower expression of bcl-2 protein and higher expression of p21 protein were observed in BM cells.When Zn2+ were added to the cell cultures before irradiating, the number of BM karyote and the contens of DNA were increased; the rates of cell apoptosis were decreased.The block of the cell cycle was improved significantly, the expression of bcl-2 protein was increased and the expression of p21 protein was decreased.   Conclusion   Zinc may protect mice BM cells against radiation damage in vitro by its effects on cell apoptosis, cell cycle and expression of genes.
2003, 19(9): 1083-1083.
Abstract(651) HTML (187) PDF 141KB(28)
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Experimental observation on germicidal efficacy of potassium ferrate solution
WANG Kai-juan, DAI Li-ping, XI Yuan-lin,
2003, 19(9): 1084-1085.
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  Objective   In order to known the germicidal efficacy of potassium ferrate solution.   Methods   Suspension and quantitative bactericidal tests were carried out.   Results   At 20℃ the solution containing potassium ferrate 10-40 mg/L and with 5-minute contact time could kill 100% of Escherchla coli and Stophylococcus aureus in suspension and its solution with 5-minute contacting time could kill over 99.50% of Candida albicans in suspension.But the solution containing 40 mg/L and with 5-minute contacting time could not kill spores of Bacillus var.niger.At 20℃, the solution containing 160 mg/L and with 30-minute contacting time could not destroy the antigenicity of HBsAg.   Conclusion   Potassium ferrate could kill bacteria under certain conditions and could kill fungus to some extent, but without efficacy on bacteria spare and HBsAg.
2003, 19(9): 1085-1086.
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Extracting, purifying and activity testing of pulmonary alveolar macrophages of mice
ZOU Dan, QUAN Hong-xun, HU Qun-yuan
2003, 19(9): 1087-1088.
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  Objective   To investigate the feasibility of extracting pulmonary alveolar acrophages(PAM)from mice.   Methods   Free pulmonary alveolar cells from mice and rats were collected by bronchoalveolar lavage fluid(BALF), and PAM were extracted by pasting.The purity of PAM were measured by acid phosphatase(ACP)assay and chicken red blood cell (CRBC)phagocytisis assay.   Results   There were no significant difference of PAM in quantity between two groups.   Conclusion   It was a simple and convenient assay of extracting PAM from mice by BALF.
2003, 19(9): 1089-1090.
Abstract(726) HTML (176) PDF 449KB(23)
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2003, 19(9): 1090-1090.
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2003, 19(9): 1091-1091.
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2003, 19(9): 1092-1093.
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Effect of prenatal lead exposure on offspring's learning ability and prevention
LIU Jian-an, LIU De-hu, ZHANG Tai-qing,
2003, 19(9): 1093-1095.
Abstract(757) HTML (161) PDF 273KB(27)
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  Objective   To evaluate prenatal lead exposure on the offspring's learning ability and protective effects of some drugs(zinc, selenite and VitC)through animal experiments.   Methods   Forty pregnant wistar rats were divided into five groups randomly: group A(double distilled water, the control groups), group B(A plus 0.1 mol/L Pb acetate), group C(B plus 0.5% VitC), group D(C plus 0.1 mol/L glucono zinc), group E(D plus food containted 0.5 μg sodium selenite), providing them drinking water and food from the first day of gestation respectively, all groups were changed to intake deionized water and common food after birth.The learning ability of the pups were tested by water-maze and down tests at the 21-day old.Pb, Zn and Se concentration of blood and hippocampus were deter mined, so were NA, DA, 5-HT and Ach concentration of cerebral cortex.   Results   (1) Prenatal lead exposure had adverse effects on the offspring's learning ability, which was probably induced by the decreased 5-HT and Ach and the increased NA, DA concentration in cerebral cortex, and which could be corrected by the supplementation of VitC, Zn and Se.(2)VitC could significantly decrease the lead level of blood, so could Zn and Se.Meanwhile, they also could significantly decrease accumulation of lead in the hippocampus of young rats.   Conclusion   VitC, Zn and Se had protective function against Pb-induced toxicity and can protect lead toxicity on the offspring's learning ability, which can gain better effect by supplementing them together.
Analysis on STD and HIV infection situation in 417 female sex workers
HE Qi-ya, LIN Jing-sheng, ZENG Xiang-Jie,
2003, 19(9): 1096-1097.
Abstract(2183) HTML (209) PDF 210KB(102)
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  Objective   To understand the infection situation of STD and HIV in sex workers and the rate of condom use in commercial sexual activities and provide scientific basis for conducting interventions on behaviours of this population.   Methods   A total of 417 sex workers were selected by random sampling method and classified into two types, working for commercial sexual activities in fixed entertainment places and finding clients beside streets.Their specimen of blood and vagina secrete were collected for HIV, syphilis and mycoplasma examination, Neisseria gonorrhoeae and candida albicans culture and diferentiation.The situation of condom use in commercial sexual activities was also investigated by questionaires.   Results   No HIV positive was detected, but the rate of the sex workers infected with one or several STD was 18.94%, among of which the infection rate of syphilis was 7.38%, gonorrhoeae 3.57% and mycoplasma 10.95%.The infection rate in sex workers on the street for clients was 50.00%, higher than that of working in fixed entertainment places.The condom use rate in the former was lower than that in the later.   Conclusion   The risk of HIV infection in sex workers on street for clients was higher than that in fixed entertainment places.
2003, 19(9): 1097-1097.
Abstract(898) HTML (314) PDF 108KB(35)
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Investigation on children's blood lead level in urban areas of Henan province
HAN Liang-feng, ZHU Gang, ZHANG Bao-ping,
2003, 19(9): 1098-1099.
Abstract(1469) HTML (228) PDF 231KB(56)
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  Objective   To investigate the blood lead level of children and to identify related risk factors in the urban areas in Henan province.   Methods   527 vein blood samples from preschool children in nine kindergarten of three cities of Henan province were sampled.Inductively coupled plasma-mass spectrometry(ICP-MS)were employed to determine lead level in whole level after microwave digestion for sample preparation and questionnare survey was also performed.A multi-variable linear regression was used to analyze the correlation between blood lead levels and risk factors.   Results   Mean blood lead contents of 527 children were 135.5 μg/L, of which 426 children(80.83%)were found with a blood lead level≥100 μg/L, and mean blood lead level of boys(138.6μg/L)was higher than that of girls(132.1μg/L), mean blood lead level of children in industrial district was 149.4 μg/L, 120.3 μg/L in non-industrial district.It showed that the more near main lines of communication and pollutant source in resident district and kindergarten, the more higher in blood lead level.It had negative effects on children-physical growth, thought judgement and expression ability.   Conclusion   Mean blood lead level of children in Henan province was significantly higher than that of adjacent province and Henan province was one of more serious areas.
2003, 19(9): 1099-1099.
Abstract(797) HTML (195) PDF 125KB(25)
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2003, 19(9): 1100-1101.
Abstract(767) HTML (218) PDF 181KB(26)
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2003, 19(9): 1101-1101.
Abstract(645) HTML (204) PDF 117KB(24)
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Analysis on high risk factor of newborn congenital syphilis in Shenzhen
FENG Si-ning, TONG Qiu-sheng, LI Fang-juan,
2003, 19(9): 1102-1103.
Abstract(839) HTML (181) PDF 207KB(25)
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  Objective   To investigate the high risk factor of newborn congenital syphilis.   Methods   53 positive and negative pairs of new borns and their mothers were selected as case group and control group of observation by rapid plama reagin circle care test(RPR)and treponema pallidum particle agglutination(TPPA).   Results   Only 12 sick mothers(22.6%)in case group had antenatal care and syphilis screening, the number was significantly lower than that of the control group (100.0%, χ2=66.861, P < 0.01)and all were pregnant syphilis.Seven mothers in case group had the treatment after 20-week pregnancy(χ2=3.087, P < 0.05), while three mothers in control group who suffered syphilis had thorough treatment before 20-week pregnancy and the rates of the accurate answer on having the antenatal care and early treatment or stopping pragnance were significantly lower than those of the control group(U=5, 408, χ2=29.754 and 10.82, Par=0.01).   Conclusion   Without enough health education, regular syphilis screening in pregnance and intervention in early stage were high risk factors of new born congenital syphilis.
Present eutrophic conditions of headwater of Jianhu lake and corresponding prevention measure
PAN Guo-shao, CAI Jian-qiang, ZHOU Xiao-ping,
2003, 19(9): 1104-1105.
Abstract(762) HTML (211) PDF 210KB(59)
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  Objective   To study the pressent eutrophic conditions of headwater of Jianhu lake in Shaoxing and provide the corresponding prevention measures.   Methods   To survey the pollution level, including sources, varieties and amounts and the pollution degree of Jianhu lake, then build a ecological mathematical model for the waterbody's eutrophication by multiple factors regression analysis.   Results   The eutrophic condition indexes(TLI)of Jianhu lake are all higher than 50, which is eutrophication.The correlative relations between the five condition variables(TP, TN, COD, Chl.a, SD)and TLI exist.TP is the the most.   Conclusion   The eutrophication of the headwater of Jianhu lake in Shaoxing is serious.Prevention measures such as controlling the pollution sources, strengthening synthetical governmnet, making long-effect controlling plans are suggested.
2003, 19(9): 1106-1107.
Abstract(781) HTML (243) PDF 384KB(33)
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2003, 19(9): 1107-1107.
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2003, 19(9): 1108-1109.
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2003, 19(9): 1109-1109.
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2003, 19(9): 1110-1111.
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2003, 19(9): 1111-1111.
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2003, 19(9): 1112-1113.
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2003, 19(9): 1113-1113.
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2003, 19(9): 1114-1114.
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2003, 19(9): 1115-1115.
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2003, 19(9): 1116-1116.
Abstract(834) HTML (228) PDF 117KB(23)
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2003, 19(9): 1117-1117.
Abstract(734) HTML (248) PDF 132KB(28)
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2003, 19(9): 1118-1119.
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2003, 19(9): 1120-1121.
Abstract(652) HTML (184) PDF 191KB(24)
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2003, 19(9): 1121-1121.
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2003, 19(9): 1122-1122.
Abstract(750) HTML (248) PDF 123KB(41)
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2003, 19(9): 1123-1123.
Abstract(690) HTML (166) PDF 126KB(19)
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2003, 19(9): 1124-1125.
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2003, 19(9): 1125-1125.
Abstract(673) HTML (213) PDF 127KB(21)
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2003, 19(9): 1126-1126.
Abstract(678) HTML (228) PDF 68KB(27)
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2003, 19(9): 1127-1128.
Abstract(693) HTML (177) PDF 188KB(33)
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2003, 19(9): 1128-1128.
Abstract(671) HTML (208) PDF 118KB(21)
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2003, 19(9): 1129-1131.
Abstract(884) HTML (494) PDF 706KB(24)
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2003, 19(9): 1131-1131.
Abstract(648) HTML (176) PDF 122KB(25)
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2003, 19(9): 1132-1134.
Abstract(1160) HTML (330) PDF 679KB(20753)
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Research on effect of air pollution on microecology of rat respiratory tract
XIAO Chun-ling, WANG Ren-qun, XI Shu-hua,
2003, 19(9): 1134-1135.
Abstract(904) HTML (245) PDF 209KB(34)
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  Objective   To explore the mechanism of respiratory tract symptom and health effect caused by air pollution and provide the theory basis for preventing air polrlution.   Methods   Animal model of wistar rat was made and rats were poisoned by imitating real air pollution, which included high, middle, lower air pollutant concentration.Bacteria flora of pharynx of rat was tested qualitatively, dynamically and position was measured.   Results   Microecology of pharynx in polluted rats appeared change.Klebsiella pneumoniae etc.pathogenic bacteria increased and anaerobe bacteria decreased(P < 0.05).The number of anaerobe bacteria and whole bacteria declined with polluted time extending.The density of pharynx bacteria flora fluctuated in a certain range and did not change with pollutant concentration.   Conclusion   Air pollution couldlead to the change of respiratory tract bacteria flora, damage the micro-environment, make normal bacteria of pharynx decreased and pathogenic bacteria planted, and it was an important factor to increase incidence of respiratory disease.
Family sex education and influencing factors in Shanghai
ZHAO Shuang-ling, GAO Er-sheng, LOU Chao-hua
2003, 19(9): 1136-1138.
Abstract(1678) HTML (584) PDF 520KB(124)
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  Objective   To identify the main problem of family sex education to provide suggestion on how to involve parents well in reproductive health promotion of adolescents and young adults.   Methods   2 675 youth aged 15-24 years old were investigated with self-administered questionnaire and 20 parents were in-depth interviewed.   Results   21.97% males and 44.35% females received sex knowledge education from parents, which was focus on hygiene during adolescence.17.36% males and 34.85% females received guidance from parents on how to make friends with peers of opposite sex.Males were less likely to receive sex education from parents, and mother played the more important role in sex education for children.Parents with the higher education level, more rigid discipline for children and the happier environment of the family was more likely to give children sex education.   Conclusion   Family sex education was far from the satisfactory, which was related with parents' inability and conservative opinion.So it was necessary to strengthen the education for parents to improve their capability and enforce their responsibility of education.Sex education for males should be emphasized.
2003, 19(9): 1138-1139.
Abstract(678) HTML (186) PDF 171KB(35)
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2003, 19(9): 1140-1141.
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2003, 19(9): 1141-1141.
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2003, 19(9): 1142-1143.
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2003, 19(9): 1143-1143.
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2003, 19(9): 1144-1145.
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2003, 19(9): 1145-1145.
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2003, 19(9): 1146-1147.
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2003, 19(9): 1148-1149.
Abstract(730) HTML (202) PDF 428KB(24)
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2003, 19(9): 1149-1150.
Abstract(780) HTML (199) PDF 188KB(36)
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2003, 19(9): 1151-1152.
Abstract(1287) HTML (553) PDF 386KB(122)
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