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2004 Vol. 20, No. 4

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2004, 20(4): 385-386.
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2004, 20(4): 386-386.
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2004, 20(4): 387-387.
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Detection of annexin V in human placenta and cultured trophoblast cells
CAO Bao-hua, MEN Ke, XU De-zhong,
2004, 20(4): 388-389.
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  Objective   To investigate the expression and the distribution of annex in V in human placentae and to find out the possible form which hepatitis B virus takes in the infection of the placental cells.   Methods   39 full-term placentae of HBsAg sero-positive and 4 full-term placentae of HBsAg sero-negative pregnant women were enro lled.Slices of placental issues and cultured trophoblasts were made for detection of annex in V by immunohistochemical staining.7 placental tissues were selected randomly for the investigation of annex in V.The immunofluorenscence assay and confocal laser scanning microscope were used.   Results   Annex in V existed in human placental tissues and located in the cytoplasm of trophoblasts, stromal cells and villous capillary endothelial cells.   Conclusion   Annexin V is plenty in human placenta and annexin V maybe a potential receptor for HBV into the trophoblast and infecting the infant.
Study on relationship between gene variation and disease progression of HIV/AIDS patients
WEN Xiao-ning, SHANG Hong, HAN Xiao-xu,
2004, 20(4): 390-391.
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  Objective   To study on the characters of HIV-1 Tat exon 1 amino acid sequence and mutations and the relationship with disease progression among HIV-1 infected people and AIDS patients.   Methods   To collect 22 cases.whole blood samples of HIV carriers and AIDS patients in Liaoning province and to extract the PBMC genome DNA.The HIV-1 tatexon 1 was amplified by nested-PCR with tat specific primers.Products were sequenced directly.Sequences were aligned, translated and analyzed.   Results   22 cases HIV-1 strains epidemic in Liaoning were found to be of genetic subtype B' and B'/C.In AIDS group, finding some amino acid mutations at specific position of tat exon 1, position 58 T substitutes A.This variation appeared only in AIDS group.In addition some other variations in position 7, 20, 24, 59, 62, 64 were found.AIDS group was higher than asymptomatic HIV-1 infection group as to these variation.   Conclusion   In AIDS patients of subtype B.and B./C, there was amino acid sequence variation of HIV-1 tat exon 1 which position 58 T substituted A, which indicated its relationship with disease progression.At the same time, some other substitution variations of tat exon 1 in AIDS patients was found, whose significance needed further research.
Study on schizophrenia susceptibility genes ochromosome 13q32
HU Ying, JU Gui-zhi, LIU Shu-zheng,
2004, 20(4): 392-394.
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  Objective   To investigate gene association with schizophrenia within 13q32 in a Chinese population.   Methods   Two single nucleot ide poly morphisms(SNPs), rs1886089 at the STK24 locus and rs2892679 at the GPC6 loucs were genotyped in 91 Chinese Han core families consisting of fathers, mothers and affected offspring with schizophrenia.These 2 SNPs were detected with the PCR-based restriction fragment length poly mor phism(RFLP)analysis.The Hardy-Weinber gequilibrium for genotype frequency distributions was estimated by the goodness-of-fit test.The transmission disequilibrium test(TDT)and the haplotype relative risk(HRR)test were applied to process genotyping data.   Results   The genotype frequency distributions of both rs1886089 and rs2892679 were in the Hardy-Weinberg equilibrium(P > 0.05);neither the HRR test nor the TDT analysis showed a genetic association between the two SNPs and schizophrenia; however, rs1886089 was associated with two of clinical symptoms(genuine auditory hallucination and apathy)(χ2=6.005 df=1 P < 0.05;χ2=6.074 df=2 P < 0.05).GPC6 rs2892679 alleles showed association with poverty of thought(χ2=6.094 df=2 P < 0.05).Aapthy and poverty of thought were two negative symptoms of schizophrenia.   Conclusion   STK24 rs1886089 and GPC6 rs2892679 were associated with three clinical symptoms of schizophrenia.More work should be done about the relation between STK24/GPC6 genes and schizophrenia.
2004, 20(4): 394-394.
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Effect of genistein on iNOS expression and anticancer in human breast carcinoma cells
LIU Ying, LU Lin, DONG Ji-sheng,
2004, 20(4): 395-396.
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  Objective   To study the genistein on the effect of proliferation and the iNOS expression of human breast car-cinoma MCF-7 cells.   Methods   MTT was used to investigate the effects of genistein in breast cancer cell proliferation.Immunocytochemstitry and RT-PCR were used to observe the effects of genistein on iNOS gene expression.   Results   Genistein inhibited the proliferation of tumor cells significantly and increased iNOS protein expression.   Conclusion   Proliferatory inhibition of human breast carcinoma cells after being treated with genistein may be due to the up-regulation expression of iNOS.
Effects of oral administration of porcine insulin on type 1 diabetes prevention and treatment in rat
REN Hong, OUYANG Feng-xiu, JIANG Qing-wu
2004, 20(4): 397-398.
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  Objective   To validate the preventive and therapy effects for type 1 diabetes with oral administration of porcine insulin.   Methods   Forty female NOD mice were divided randomizedly into four groups.Begining from four, eight and twelve-week age, tree groups were orally administered porcine insulin 700 l(1 mg)and other 500 l PBS only.Twice before the twelfth week and then once weekly until 35-week age.   Results   When the mice were thirty-five-week age, the cummulative incidences of all insulin groups were significantly lower than the control group(P < 0.01), but the survival rates were significantly higher than the control one(P=0.009).   Conclusion   Oral administration of porcine insulin can not only prevent but also cure the type 1 diabetes.
Analysis on inspection of influenza in Guangdong province from 1991 to 2000
NI Han-zhong, ZENG Han-wu, LIU Shao-mei,
2004, 20(4): 398-399.
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  Objective   To understand the situations of influenza epidemic as well as the aberrance of the surface proteins antigenicity of influenza virus.   Methods   Influenza virus isolation was performed by using the erythrocy test eggs aging form 9 to 11 days or MDCK cells.The identification of virus and the verification of serum influenza antibody of hen were carried out with hemagglutinin inhibition test and semi-sensitried mircro-hemagglutinin inhibition test separately.   Results   There was no massive influenza epidemic outbreak from the year 1991 to 2000.1008 strains were isolated in such period.A(H3N2) subtype influenza virus(62.3%)and B type influenza virus(26.6%)was in dominance.10 strains of H9N2 subtype of birds influenza virus were isolated form human groups.98 strains of H9N2 birds influenza virus were isolated form chickens.The antigenicity of A(H3N2)subtype virus were floating continuously.   Conclusion   Subtype of A(H1N1)could not be isolated in the year 1993 and 1995, but A(H1N1), A(H3N2)and B type influenza virus could be isolated contemporary during the rest 8 years.Subtype of H9N2 birds influenza virus could be isolated both in the year 1998 and 1999.The variation in surface antigen of A(H3N2)subtype could be confirmed.There were couples of A subtype of influenza viruses occurred temporary in chickens.It was necessary to carry out influenza inspection.
Improvement on conditions of PCR/SSCP and its application in rapid genotyping of orientia tsutsugamushi
LIU Yun-xi, ZHAO Zhong-tang, GAO Yuan,
2004, 20(4): 400-402.
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  Objective   In order to rapidly analyze the gene type of orientia tsutsugamushi(Ot)from Shandong province by PCR/SSCP.   Methods   Comparing the SSCP maps of the amplification products by group primers of Ot-DNA acquired from 23 strains of Ot isolated, 2 pools of homogenized Leptotrombidium(L.)scutellare, and 10 blood of scrub typhus with the reference strains Gilliam、Karp、Kato, and also compared with the results acquired by Nested PCR.   Results   Ot-Sta56 gene amplification fragments by group primers of Ot-DNA acquired from 23 strains of Ot isolated, 2 pools of homogenized L.scutellare, and 10 blood of scrub typhus appeared two different maps of SSCP, 33 had the same gene types different from that of the reference strain Gilliam、Karp、Kato, 2 had the same gene type with the reference strain Karp.This results were identical to that acquired by Nested PCR.   Conclusion   PCR/SSCP was a rapid, simple, accurate method for analyzing the gene type of Ot.
2004, 20(4): 402-403.
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Immunotoxicologic assessment on transgenic rice
LI Ying-hua, PIAO Jian-hua, CHEN Xiao-ping,
2004, 20(4): 404-406.
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  Objective   To observe the immunotoxicologic effects of transgenic rice on mice.   Methods   The Balb/c mice were divided into four groups, transgenic rice group(Xa group), non-transgenic rice control group(N group), AIN93G control group(A group)and positive control group(P group).The mice were fed with corresponding food for 30 days.The immunity indicators of mice were compared.   Results   The indicators were as fellows: body weight, blood routine test, lymphocyte sort, lymphocyte transform test, immunity organ index, delayed hypersensitivity response, antibody forming test, serum antibody titter, macrophage function test and NK cell activity test.All indicators between Xa group and N group were no statistic difference.The spleen index and NK cell activity in Xa group were higher than those in A group and the other indicators were no statistic difference each other.Most of all indicators in P group were lower than that in the other groups.   Conclusion   The immunotoxicologic effects of transgenic rice on mice were safe.
Modified method for determination of aceclofenac in plasma
XIE Hong, QIU Xiang-jun, WANG Gui-zhen
2004, 20(4): 406-407.
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  Objective   A modified method based on high performance liquid chromatography was developed for the determination of aceclofenac in human plasma.   Methods   Aceclofenac was extracted from plasma with diethylether.The analytical column was packetd with Hypersil-C18(4.6 mm×250 mm, 10 L).The mobile phase was methanol-water-glacial acetic acid(73B30B0.5, V/V)and the flow rate was 1.5 ml/min.The UV detection wavelength was 276 nm.   Results   The calibration curve was liner in the range from 0.1 to 100μg/ml(r=0.996 1), with determination limit of 0.1 μg/ml and the average recovery of(101.17±1.17)%.The intra-day RSD and inter-day RSD were(6.04±2.45)%and(5.01±1.39)%, respectively.   Conclusion   The method was simple, rapid, accurate and can be used to determine the aeclofenac concentration in plasma and for pharmacokinetics study.
Study on Borna disease virus natural infection in China and analysis of sequences
MA Pei-lin, ZHANG Feng-min, LI Gui-mei,
2004, 20(4): 408-410.
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  Objective   To investigate the situation of BDv infection among normal persons and died horses in Heilongjiang area and demonstrate the relationship between these isolates with those from the schizophrenia patients and horses in the world.   Methods   Nested RT-PCR was used to detect BDV p24 gene from PBMCs of normal persons and horses in Heilongjiang area, and two positive nested-PCR products of each specimen were cloned and sequenced Then the sequences were compared with those of the isolates from schizophrenia patients and horses in the world   Results   7 of 76 persons (9.2%)and 8 of 34 horses(23.5%)were found to be BDV-p24 positive.Exceptwiththe strain No/98.these nested RT-PCR products were highly conservative with the isolates from schizophrenia patients and horses in the world. At the nucleotide level, the divergenoas between the products and these isolates were all below 5%.and the conservation between these products with the standard strain of He/80 was the highest, more than 98%.   Conclusion   BDV infection existed in normal persons and horses in Heilongjiang area.BDV irffecting the normal persons and horses in China was highly conservative with the standard strain of Fie/80.
2004, 20(4): 410-410.
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Cytotoxicity of microcystin-LR on cultured cells
GU Kang-ding, LIN Qun-xin
2004, 20(4): 411-412.
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  Objective   Cell lines were empolyed to explore the possibility of taking permanent cell lines as models for detecting microcystins and studying their mechanism of cytotoxicity.   Methods   Eight kinds of cell strains from different hosts (KB, NIH/3T3, H-4-II-E, Hela, Vero, HepG2, Caco-2, HL-60 cell)were mixed with purified microcystin-LR and incubated for 24, 48, 72 and 96 hours; Meanwhile morphological alterations, colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT)assays, lactate dehydrogenase(LDH)release assay were respectively used to evaluate the end point of toxicity.   Results   Among 8 cell lines, KB and H-4-II-E cell lines showed significant dose-response effect at toxin concentrations greater than 18.8μg/ml with 96-hour incubation.In KB cells the toxin induced marked morphological alterations after incubation for 8 hours which was with good reproducibility and consistency.LDH assay results showed that microcystin-LR caused cell membrane damage.   Conclusion   Purified microcystin-LR did induce toxic effect on KB and H-4-II-E cell lines.However, considering morphological changes, reproducibility and consistency, KB cells seemed to be a better potential model for evaluating cytotoxicity of microcystin-LR.
Study on rat fetuses talipes equinovarus model induced by all-trans retinoic acid
ZHOU Hai-tao, SUN Kai-lai, LI Zeng-gang,
2004, 20(4): 413-415.
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  Objective   To establish rat fetuses talipes equinovarus model induced by all-trans retinoic acid and investigate the optimum dosage of all-trans retinoic acid.   Methods   Wistar rats were distributed randomly into a control group and 4 assay groups.On day 10 of pregnancy, variable dose of 120, 130, 135 and 140 mg/(kg°bw)all-trans retinoic acid suspended in miner al oil was given intragestrically to the rats of each assay group and equivalent volume of mineral oil of the 140 mg/(kg°bw)group was given to the control rats.Fetuses were delivered and examined under stereomicroscope on day 21 of pregnant.The hindlimbs were removed, processed, sliced up as serial sections in three planes of foot, analysed under a light microscope.   Results   Deformity of talipes equinovarus and other external malfomlations were induced by all-trans retinoic acid on Wistar rat fetuses.The incidence of simplex deformity of talipes equinovar us was the highest in the 135 mg/(kg°bw)assay group.   Conclusion   135 mg/(kg°bw)all-trans retinoic acid was the optimal dosage for inducing deformity of talipes equinovarus.
2004, 20(4): 415-415.
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Tea polyphenols alleviated endothelial hyperpermeability induced by angiotensin Ⅱ
YING Chen-jiang, SUN Xiu-fa, ZHO Xue-zhi,
2004, 20(4): 416-417.
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  Objective   To observe the effects of tea polyphenols on endothelial cell hyperpermeability induced by Ang Ⅱ and search for the mechanism of tea polyphenols on down-stream redox signal mediated by Ang Ⅱ receptor typeⅠ(AT1).   Methods   The intracellular superoxide anion production in BACECs challenged with AngⅡ(1 mg/L)was measured by quantitative nitroblue tetrazolium(NBT)assay and the extracellular hydrogen peroxide concentration was determined after the cells were pre-incubated with GTP or BTP(0.4 mg/L)for 24 h.In cultured monolayer BCA ECs, permeable FITC- dextran(2.5μmol/L)was measured to evaluate the effects of tea polyphenols on the changes of AngⅡ-induced endothelial permeability.   Results   Both green tea and black tea polyphenols diminished the intracellular superoxide anion induced by Ang Ⅱ(P < 0.05), and alleviated the intercellular permeability induced by Ang Ⅱ(P < 0.05).The similar permeability decrease induced by Ang Ⅱ was also obtained using either NADPH oxidase inhibitor DPI or antioxidant VE pretreatment.   Conclusion   Tea polyphenols alleviated Ang Ⅱ-induced hyperpermeability-mainly via decreasing the ROS production.
2004, 20(4): 417-418.
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Analysis on diesel oil vehicle exhaust and its oxidative DNA damage
XI Zhu-ge, CHAO Fu-huan, SUN Yong-mei,
2004, 20(4): 419-421.
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  Objective   The genotoxicity and its molecular mechanism of diesel oil vehicle exhaust were discussed through analysing the oxidative DNA damage induced by exhaust components.   Methods   DNA adduct 8-hydrodeoxyguanosine(8-OHdG)was used as a biomarker of oxidative DNA damage.The level of 8-OHdG in DNA exposed to diesel oil vehicle exhaust was detected by HPLC-EC in vitro.Organic and inorganic components in gasoline vehicle exhaust were analyzed by GC-MS and AAS respectively.The molecular mechanism of oxidative DNA damage was discussed by these components.   Results   It was found that there were 108 and 9 kinds of organic compounds, 8 and 6 kinds of inorganic compounds in particles and volatile organic compounds(VOCs)of diesel oil vehicle exhaust.In addition, particles and VOCs of diesel oil vehicle exhaust could directly induce oxidative DNA damage, and had the dose-response relationship.   Conclusion   The main reason of the oxidative DNA damage caused by diesel oil vehicle exhaust was that the diesel oil vehicle exhaust containd a lot of polyphenols and quinones compounds and some trace metal ions, which can further produce large amounts of superoxide (O2)and hydroxyl radicals(°OH), to attack DNA and form 8-OHdG.
Investigation on cardiovascular risk factors in 7660 adults in Liuzhou
HU Shi-hong, YANG Jing, JIA Wei-hong,
2004, 20(4): 421-422.
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  Objective   To evaluate condition of cardiovascular risk factors in population of Liuzhou in order to provide base investigation for intervention.   Methods   A cohort of the 7 660 residents of Liuzhou were selected sampled in group, average aged(39.5±11.0)years.The demographic data of the inhabitants and laboratory examinations were investigated.Cardiovascular risk factors included blood pressure, height, weight, waist circumference(WC), fasting blood glucose and lipid of 7660 cases were detected and statistically analyzed.   Results   The detecting rate of hypertension, impaired fasting glucose, diabetes mellitus(DM), hypercholesterolemia, hypertriglyceridemia, overweight, obesity and large WC were 16.64%, 6.50%, 21.51%, 20.76%, 26.37%, 31.44%, 7.92% and 23.75%, respectively.   Conclusion   The detecting rate of cardiovascular risk factors in this population were higher.The intervention measures should be adopted in order to decrease the risk factors and reduce occurrence of developing of their cardiovascular diseases.
Detection of shiga toxin 2 gene by ramification amplification method
ZHAO Chun-yan, LI Fan
2004, 20(4): 423-424.
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  Objective   To develop a rapid and sensitive assay to detect shiga to xin-producing escherichia.coli.   Methods   The sensitivity and specificity of RAM were compared with those of PCR by detecting synthetic Shiga toxin DNA target and isolated from food and clinical specimens.   Results   The lowest number of targets detected by RAM assay was 10 molecules.Thirty-three strains from clinical samples and foods were detected by RAM and PCR, twenty-six among of which were positive for stx 2 gene.The results of both methods were the same.   Conclusion   RAM assay could be an alternative to PCR to detect STEC in food product and clinical specimens because of its high sensitivity and specificity, simplicity and isothermal amplification.
Effect of nonylphenol (NP) on mRNA and protein expression of in ovarian cancer
ZHAN Ping, LÜ Bo, LI Yuan-feng
2004, 20(4): 425-426.
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  Objective   To explore the effects of nonylphenol(NP)on mRNA and protein expression of c-myc and p53 in ovarian cancer PEO4 cells.   Methods   Human ovarian cancer PEO4 cells were grown in DMEM medium.MTT assay was employed to investigate cell proliferation.RT-PCR and immunohistochemistry was performed to determine mRNA and protein expression of c-myc and p53.   Results   Within the range of(8-96)×10-7 mol/L, NP stimulated proliferation in PEO4 cells with a dose-dependent and time-dependent fashion.RT-PCR and immunohistochemistry analyses revealed that treatment with 32×10-7 mol/L for 72 h resulted in increased mRNA and protein expression of c-myc and p53 with 2-3 fold.   Conclusion   NP possessed estrogenic activity and could markedly stimulate proliferation in estrogen responsive ovarian cancer PEO4 cells.Up-regulation of c-myc mRNA and protein expression was suggested to be involved in this biological effect.
Extraction and dynamics study on antibacterial protein of housefly larva by inducements
LIU Hui, WAN Qi-hui, ZHANG Xi,
2004, 20(4): 427-428.
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  Objective   To conduct comparative studies on antibacterial activity of antibacterial protein from the induced housefly larva by two methods, and observation of dynamic changes of antibacterial protein.   Methods   The housefly larva were injecteded by E.coli and S.aureus, compared with antibacterial activity of antibacterial protein from haemolymph and tissue homogenate after being induced 12, 24, 36, 48, 60 h.   Results   Antibacterial protein from tissue homogenate was more antibacterial activity than that from haemolymph.After induced 48 h by S.aureus, antibacterial protein of housefly larva was more active against bacteria, but after induced 36 h by E.coli, antibacterial protein was more activity.   Conclusion   Extration of antibacterial protein from tissue homogenate was a better method, and antibacterial protein was more activity after induced 36-48 h.
Study on related factors of serum NPY level in children with simple obesity
HUA Tian-yi, XU Chun-xiu, JIANG Mei,
2004, 20(4): 429-430.
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  Objective   To investigate the related factors of serum NPY level in children with simple obesity.   Methods   Serum NPY level was measured by radioimmunological simple obesity children aged 6-13.   Results   No relationship was observed between serum NPY level and age.No different NPY level was found between the male and female.NYP level was increased significantly in moderate and severe obesity than that in mild one, corr elated positively with body weight.And NPY level elevated with the increase of body weight.NPY level in children with hyperlipidimia or family obesity history increased significantly than that in without.Children with a high frequency of food intake and long obesity history(≥5 years)elevated NPY levels than those with normal frequency and short history(< 3 yeas).   Conclusions   Serum NPY level in simple obesity children was related to body weight, family obesity history, frequency of food intake and the course of obesity, but not to age and sex.
2004, 20(4): 430-430.
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Study on GSTM1 genetic polymorphism in traffic police population in Guangzhou
CHU Xin-wei, ZHU Wen-chang, CHEN Qing,
2004, 20(4): 431-432.
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  Objective   To investigate the polymorphism distribution of the GSTM 1 genotypes in traffic police population in Guangzhou.   Methods   The epidemiological data were collected by a cross-sectional study and GSTM 1 polymorhisms were determined by the multi-differential polymerase chiain reaction(MD-PCR)method using the samples of peripheral blood leukocyte DNA.Individuals were stratified according to their sex, native place, race, cancer family history, job and duration of exposure.The frequency of GSTM 1-null genotype in traffic police population in Guangzhou was compared with that of other population in China.   Results   The frequency of GSTM 1-null genotype came to 59.58% in the study population, which was higher than that of urban population in Shanghai normal population and lower than that of lung cancer population in Yunnan (P < 0.05).However, no significant differences were found between GSTM 1-mull genotype and sex, nationality, work group, family cancer history, length of service and work environment, exposed automobile more than 10 hours per week(P > 0.05).   Conclusion   Distribution of the GSTM 1 genes was polymorphic and GSTM-null genotype was similar in the characteristics of the study population.
Construction of recombinant plasmid pQE32-HPV18L1 and protein expression
LI Di, GU Hong-xi, ZHUANG Min,
2004, 20(4): 433-435.
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  Objective   To construct a recombinant prokaryotic expression vector for obtaining the HPV18L1 protein and develop the genetic engineering vaccine.   Methods   The L1 gene of HPV18 was amplified by PCR from DBR322-HPV18 and cloned into pUC19.The sequence of cloned HPV18L1 was confirmed by restriction analysis and DNA sequencing.A HPV18L1 prokaryotic expression plasmid, pQE32-HPV18L1, was then constructed by subclone.pQE32-HPV18L1 tranformed E.coli M15 was induced by IPTG.The expression of L1 protein was analyzed by SDS-PAGE and western blot.   Results   The amplified DNA fragment was in size of 1.7 Kb as expected.Restriction analysis showed that the amplified gene was inserted in pUC19 correctly.Sequence showed there was no mutation in both ends of the cloned L1.Restriction analysis showed that the recombinant pQE32-HPV18L1 right.A63KD protein could be seen in M15 induced by IPTG with SDS-PAGE and was positive while reacting with HPV18L1 antibody by western blot.   Conclusion   HPV18L1 was successfully cloned and expressed in this study.
Analysis of risk factors of excessive internet using among university students
LÜ Yuan, YI Yin-sha, DENG Chang,
2004, 20(4): 435-436.
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  Objective   To investigate the risk factors of excessive internet using.To provide the advice for the teaching staff of higher education to induct university students appropriatly using internet.   Methods   In randomily stratified and cluster selected 1 000 students in Central South University, Hunan Normal University, Changsha University.12 faotors surveyed and data which dependent variable is curative effect is analyzed by single and multipe logistic regression.   Results   Exclusive internet using is related on educational level, administrative level, interest of speciality, anxiety symptom, fear symptom, family relation etc.   Conclusion   The result analyzed by multiple logistic regression indicated that family relation and anxiety symptom are risk factors of excessive internet using, administrative levels, interest of speciality and study achievementhad protective effect on excessive internet using.
Effect of smoking on endocrine gland function of female and related factors
SONG Yan-ping, SU Lu, YIN Hong-bo,
2004, 20(4): 437-438.
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  Objective   To understand the influence of smoking on feminine endocrineg land function and the micro element metabolism and the oxidizing function.   Methods   To collect punctately the urine and blood of women whose ages ranged from 30 to 40, who lived in some north suburb continually over 5 years and smoked over 2 years and did not take or ally the contraceptive drug, to test the volume of thyroid and FT4, FT3, TSH and estradiol(E2), etc.   Results   The FT4 and E2 of the smoking women were 10.9% and 13.6% lower(Par < 0.05)than the control group respectively.The FT3 and TSH increased 74.4%(P < 0.01)and 10.9%(P < 0.05);the iodine and GPx and selenium decreased 11.6%(P < 0.05)and 15.7% and 22.4%(Par < 0.01);the lead and cadmium and LPO increased 25.4% and 160.2% and 31.1%(Par < 0.01); the value of r of the lead iodine and selenium were-0.683 and-0.537(Par < 0.01)respectively; the value ofr of selenium and FT3 and GPx were-0.688 and-0.546(Par < 0.01)respectively; the value of r of the LPO and E2 were-0.446 and (P < 0.05)and-0.659(P < 0.01).   Conclusion   Smoking could interfere the coordination of the feminine endocrine gland function, which was concerned with the free radical caused by the poison and the inhibition against the absorption of micronutrient.
2004, 20(4): 439-440.
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Protective effects of glutathione on cisplatin-induced toxicity to rat hepatocytes
LU Yong-ke, KAWASHIMA Akira, HORII Ikuo,
2004, 20(4): 440-441.
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  Objective   To investigate the effects of glutathione on cisplatin-induced toxicity to rat hepatocytes.   Methods   Hepatocytes were isolated from rats and then inoculated into 96 well plates, after pretreatment of L-cysteine(precursor of GSH synthesis)or BSO(inhibitor of GSH synthesis)for 4 hours and 16 hours, respsectively.A series of concentrations of cisplatin, in the presence and absence of L-cysteine, were added and coincubated, MTT assays were performed to test cell viability at 8, 24 or 48 hours after coincubation.   Results   Cisplatin had time-dependent and concentration-dependent cytotoxicity.The concentrations of cisplatin that inhibited 50% cell growth(IC50)of rathepatocytes at 8, 24 and 48 hours were 1.13, 0.21 and 0.15 mmol/L, respectively.BSO made IC50 of cisplatin to rathepatocytes lower down to 0.017, 0.011 and 0.013 mol/L, respectively, while L-cysteine made IC50 increase up to more than 5 mmol/L.   Conclusion   Cisplatin has time and concentration-dependent toxicity to rathepatocytes; BSO can enhance cisplatin-induced cytotoxicity, while L-cysteine can protect cisplatin-induced cytotoxicity.It was possible that GSH can protect cisplatin-induced toxicity to rathepatocytes.
Protective effect of bilirubin and natural cow-bezoar on styrene-induced HepG2 cell DNA strand breaks
WEI Xue-tao, JIANG Jian-jun, SHANG Lan-qin,
2004, 20(4): 442-443.
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  Objective   To observe the DNA strand breaks in HepG2 cell induced by styrene and to evaluate the antagonistic effect of natural cow-bezoar and bilirubin on DNA strand breaks induced by styrene.   Methods   Using SCGE(Single Cell Gel Electrophoresis)to detect the DNA strand breaks in HepG2 cell in vitro.   Results   Under the styrene concentration that the viability of HepG2 cell was not influenced, 0.2 μmol/L styrene could induce apparent DNA strand breaks in this kind of cell.The frequency and the migration of "comet" cell was(9.5±2.1)% and(4.5±0.7)μm, respectively.At the styrene concentration that could induce apparent DNA strand breaks natural cow-bezoar or bilirubin was added at the same time.Both natural cow-bezoar and bilirubin could reverse the DNA strand break induced by styrene in certain concentration.The co-treatment of 1 μmol/L styrene and 10 μmol/L natural cow-bezoar or bilirubin could counteract the effect of styrene almost thoroughly.   Conclusion   Styrene could induce DNA strand breaks in HepG2 cell and both natural cow-bezoar and bilirubin could antagonize such damage effect of styrene.
Detection of vibrio cholerae with DNA microchips
JIN Hui-ying, TAO Kai-hua, LI Yue-xi,
2004, 20(4): 444-445.
Abstract(824) HTML (248) PDF 406KB(73)
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  Objective   Rapid specific and sensitive DNA microchips are used for detection of vibrio cholerae.   Methods   Primers and specific probes were designed from vibrio cholerae gene ompW and ctxA.The fluorescent-labeled PCR products were prepared by incorporation of Cy3-labeled primer during PCR amplification and were hybridized to the microchips for detection of vibrio cholerae strains and non vibrio cholerae pathogens.   Results   Positive signal were all displayed on the mycrochips in vibrio cholerae strains when used conventional PCR or multiplex PCR, but non vibrio cholerae pathogens failed to yield any signal under comparable conditions.   Conclusion   DNA microchips provide a more specific, rapid and efficient method for vibrio cholerae detection.It might be very useful for high-throughout, integrated and automated identification and detection of bacterial pathogens by designing more probes.
Effects of laminaria hemicellulose on thrombus formation in aged rats
XIE Lu, LI Jing, HU Shi-feng
2004, 20(4): 446-447.
Abstract(817) HTML (291) PDF 371KB(44)
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  Objective   To study the effect of laminaria hemicellulose on thrombus formation.   Methods   The occlusion time and the weight of thrombus were compared between laminaria hemicellulose group and control group respectively through three rat models of thrombus formation after laminaria hemicellulose was injected intraperitoneally(i.p)for three days.   Results   Both low dose group of laminaria hemicellulose(20 mg/kg)and high lose group of laminaria hemicellulose (100 mg/kg)can obviously prolong the occlusion time and decrease the weight of thrombus.   Conclusion   Laminaria hemicellulose can inhibit the thrombus formation in rats.
2004, 20(4): 447-448.
Abstract(714) HTML (307) PDF 352KB(34)
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Comparison study between cyclophosphamide and homocysteine to cause rat's NTDs
LI Xue-min, XIAO Rong, ZHAO Hai-feng
2004, 20(4): 449-450.
Abstract(781) HTML (233) PDF 680KB(29)
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  Objective   To comprise the neural tube defects teratogenicity effects of cyclophosphamide and homocysteine.   Methods   The pregnant rats were randomly divided into control and fourcy clophosphamide experimental groups and three homocysteine experimental groups on their bodyw eight.The cyclophosphamide intakes among four groups were 7.5, 10.5, 12.5, 15.0 mg/kg via intraperitoneal administration on the 13th day of gestation, and the homocysteine intakes among three groups were 600, 300, 150 mg/kg via mouth during pregnance.On the 14th day of gestation, three rats were randomly killed and embryos were checked through optical microscopy and transmission electron microscopy; The other rats were killed on the 20th day of gestation.   Results   The group of 12.5 mg/kg had a high frequence of neural tube defects and a low frequence of death, the results from optical microscopy and transmission electron microscopy indicated some typical changes of apoptosis.But the positiveteratog enicity effects of homocysteine on rats were not be observed in homocysteine experimental groups.   Conclusion   To make a model of neural tube defects, the intake of 12.5 mg/kg was feasible; neurons apoptosis might be involved in its teratogenesis, while rat was not sensitivity to homocysteine.
Development and application of PCR kit for rapid detection of salmonella
HUANG Jin-lin, JIAO Xin-an, LIU Pei-hong,
2004, 20(4): 451-452.
Abstract(732) HTML (237) PDF 214KB(76)
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  Objective   To develop a polymerase chain reaction(PCR)diag nostic kit for rapid detection of salmonella.   Methods   Based on the effects of PCR detection, it was determined the components of PCR kit, and evaluated their affects on storage-life of kit.Meanwhile, the accuracy of both ELISA-kit and PCR-kit was compared.   Results   The detection threshold of PCR kit was determined as 20 pg of salmonella DNA template.The storage-life of PCR-kit containing Taq DNA polymerase is longer than 12 months when it was stored at -20℃.The sensitivity and specificity of PCR method reached both 100%, while that of direct ELISA was 100% and 97.2%, respectively.The coincidence of these two methods reached 97.2%.150 human feces samples were detected by the PCR-kit and the results was coincided well with that of national standard(GB)method.   Conclusion   PCR-kit was highly specific, sensitive and quick, it would be very useful in the rapid detection of salmonella.
2004, 20(4): 452-452.
Abstract(546) HTML (204) PDF 130KB(36)
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Effects of VC and VE on toxicity of mercury
YANG Jing-hua, XU Zhao-fa, YIN Zhong-wei,
2004, 20(4): 453-454.
Abstract(952) HTML (241) PDF 1299KB(51)
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  Objective   To study the effects of vitamin C(VC)and vitamin E(VE)pretreatment on the toxicity of mercury(Hg).   Methods   Forty-eight Wistar rats were divided into six groups randomly, control group, three Hg groups, VC or VE pretreatment groups.At 48 hours after the last injection, the animals were sacrificed, and the liver and renal cortex were collected.Hg, malondialdehyde(MDA), glutathione(GSH)contents and glutathione-peroxidase(GSH-Px)activities in the liver and renal cortex were measured.   Results   Hg contents in the liver and renal cortex increased with Hg dose increasing, and high-dose Hg group was significantly higher than the control group.Hg contents in the renal cortex of all groups were higher than those in the liver.GSH contents and GSH-Px activities in the liver of middle-dose Hg group were higher significantly than the control values.GSH contents and GSH-Px activities in the renal cortex of low and middle dose Hg groups were higher significantly than the control values.MDA contents in the renal cortex of middle and high dose Hg groups were higher significantly than the control values.MDA contents in the renal cortex of VC and VE pretreatment groups were lower significantly than those of the high-dose Hg group.GSH contents, GSH-Px activities in the renal cortex and GSH contents in the liver of VC and VE pretreatment groups increased significantly as compared with those of the control and high -dose Hg groups.   Conclusion   VC and VE could protest against the toxicity induced by Hg.
Mycardium injury of chronic transdermal cigarette smoke extracts(CES) on mice
YANG Yu-mei, LIU Geng-tao
2004, 20(4): 455-456.
Abstract(722) HTML (307) PDF 480KB(32)
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  Objective   To determine whether chronic exposure to cigarette smoke extract(CES)in mice could induce myocardium toxicity and to clarify whether nicotine in cigarette smoke is responsible for this impaired myocardium response.   Methods   Transdermal injection of 50%, 40% CES 10 mg/kg twice daily or nicotine 1.5 mg/(kg°day)for 12 weeks.   Results   Prolonged CSE administration caused a dose-dependent inhibition of cytochrome coxidase activity〔(424.75±66.25)vs (690.15±68.20)nmol/(mg°min), P < 0.01〕, while no significant changes in plasma GOT, LDH and CPK.Nicotine showed no significant damage on mouse heart mitochondria in vivo.Heart weight of the CSE-treated mice was significantly increased as compared with normal control group〔(0.29±0.05)vs(0.23±0.02)g, P < 0.05〕.The CSE exposure induced mitochondria was swollen with inner membrane damage, disruption of cristae, vacuolization of the matrix by transmission microscopy.   Conclusion   Chronic exposure of mice to CSE caused inhibition of myocardial cytochrome C oxidase activity as well as mitochondrial structure damage.Nicotine was not the responsible component for the toxicity of CSE to myocardial mitochondria.
VZV shape change in 2BS cell infected HAV
LIU Jian-hua, XIN Zhong, ZHANG Jin-yan,
2004, 20(4): 457-458.
Abstract(854) HTML (277) PDF 441KB(47)
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  Objective   To analyze VZV shape changing process after being infected HAV 2BS cell.   Methods   Infecting VZV after 2BS being infected HAV 21 days was trial group, to gather cell at different time to make the electron microscope specimen by section and negative dye to adopt 2BS cell single infected VZV or HAV for control group.Observing the sample under electron microscope.   Results   VZV can infect 2BS cell again which was infected HAV and the offspring virus may be completely produced.VZV multiplication was interfered by HAV.The increasing amount of offspring virus was less than single-infected VZV or HAV and the reproducing circle prolonged.
Study on inhibiting effect of ATRA and cisplatin on human hepatoma cell
LI Wei-dong, ZHANG Ming-lei, PU Yun-feng
2004, 20(4): 459-460.
Abstract(779) HTML (297) PDF 398KB(37)
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  Objective   To investigate the mechanism of effect of A TRA and cisplatin on telomerase activity and proliferation of hepatoma.   Methods   Cell culture, morphology MTT calorimetric assay and TRAP assay.   Results   ATRA is the only one that can inhibit telomerasa activity, all agents can inhibit the proliferation of hepatoma, the inhibitory effect of cisplatin is the strongest.   Conclusion   ATRA can inhibit obviously telomerase activity of hepatoma cells.
Analysis on children's psychomotor state among different IQ levels in iodine deficiency disorders regions
YIN Hong-bo, CHEN Xin-qiu, LIU Lian-jie,
2004, 20(4): 460-461.
Abstract(725) HTML (199) PDF 215KB(19)
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  Objective   To evaluate the level of children's IQ and psychomotor in different iodine deficiency disorders regions.   Methods   Intelligence quotient(IQ)was measured by Raven Progressive Matrix(CRT-C2)and psycho motor were detected by Jin Yi Psychomotor Test Battery(JPB).   Results   The distribution of children's IQ levels was significantly different in the severe, moderate and mild regions(P < 0.01), and the intelligent retarded rates were 2.34%, 4.52%, and 0.99%, respectively.The intelligent retar ded rates of the severe or moderate region were significantly hig her than that of the mild region(P < 0.01).The results of T value showed that abnormal rates of psy chomotor had significant difference between the severe, or moderate, mild regions, and the abnormal rates were 12.89%, 13.57%, 4.93%, respectively.The abnormal rates had significant difference(P < 0.01)among different regions between different IQ levels.The abnormal rates increased with the IQ levels decreased.   Conclusion   The levels of children's IQ and psycho motor in the severe and moderate iodine deficiency disorders regions were significantly lower than those in the mild region.The abnormal rates of psycho motor increased with the IQ levels decreasing.
2004, 20(4): 462-463.
Abstract(628) HTML (222) PDF 213KB(25)
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2004, 20(4): 463-463.
Abstract(642) HTML (238) PDF 132KB(30)
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2004, 20(4): 464-464.
Abstract(497) HTML (171) PDF 79KB(28)
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Survey on psychological health of rural women
HE Chao-yang, XU Hua, XIAO Xia
2004, 20(4): 465-466.
Abstract(910) HTML (258) PDF 356KB(54)
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  Objective   To understand status of psychological health of women in rural area.   Methods   Cluster random sample was applied to select adult women in three natural villages.SCL-90 scale was used to quantity survey to their status on psychological health.   Results   The score of all factors in SCL-90 was higher than that of female normal standard except factors of personnel relationship and compulsion.And there were high significant difference(P < 0.01)between two group people.There was no significant difference(P > 0.05)among age stages.Most high score occurred in young group.   Conclusion   Women living in countryside of Jinning county had some psychological and physical problem.Relevant departments should pay attention to this issue and strengthen education of health and psychological consult to them.
Analysis on prevalence and risk factors of chronic disease in residents of Dandong
DONG Guang-hui, LIU Qi-nan, TENG Yue-zhou,
2004, 20(4): 467-468.
Abstract(1200) HTML (392) PDF 200KB(47)
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  Objective   To study the prevalence and behavioral risk factors for chronic disease in city of Dandong.   Methods   The adults aged over 20 years were investigated abouthealth condition and behavioral risk factor with stratified sampling.   Results   The prevalence of hypertension, hyperlipedimia and coronary heart disease(CHD)were 15.51%, 31.53% and 8.22% respectively.The rates of smoking, drinking, diet with hyper salt, overweight and insomnia were 25.65%, 19.60%, 17.77%, 29.26% and 19.73% respectively.Multi-variable analyses showed that over weight, fat, smoking, drinking, diet with hyper salt were significantly associated with hypertension, hyperlipedimia, CHD, stroke, Diabetes.The more years one drinked and smoked, the higher risk one suffered from chronic disease.Prevalence rates of CVD, stroke, and diabetes raised higher among persons with overweight accompanying hyper tension and hyperlipidemia than healthy group.   Conclusion   Hypertension, hyperlipedimia and CHD were main chronic disease in Dandong rural.Health education, stop smoking and hypertension control were the major intervention measures.
Analysis on risk factors of sensory integration dysfunction
CAO Xiu-jing, TAO Fang-biao, ZHANG Hong-bo,
2004, 20(4): 469-470.
Abstract(849) HTML (255) PDF 477KB(77)
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  Objective   In order to understand the status of sensory integration dysfunction and the influencing factors in children.   Methods   Child Integration Scale and self-made questionaire of influencing factors were applied to investigate 933 pupils aged 6 to 11.   Results   The total rate of sensory integration dysfunction was 34.7%.The rate in boys was higher than girls significantly(42.3% and 28.8% respectively).The main risk factors of child sensory integration dysfunction were: study experience of mother, family's format and circumastus, the attitude and ways of parents in instructing children, the condition before pregnancy, children's illness before school and kinship care.   Conclusion   Sensory integration dysfunction was an end of multiple factors and multiple intervention approaches were necessary for prevention.
2004, 20(4): 470-470.
Abstract(596) HTML (174) PDF 126KB(26)
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2004, 20(4): 471-472.
Abstract(668) HTML (190) PDF 191KB(29)
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2004, 20(4): 472-472.
Abstract(624) HTML (203) PDF 122KB(24)
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Relationship between lipid metabolism and risk factors of cardiovascular disease in adults
WANG Ning, XIE Zi-jing, Abulikemu,
2004, 20(4): 473-474.
Abstract(743) HTML (261) PDF 526KB(24)
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  Objective   To study the relationship between special characteristics of lipid metabolism and risk factors of cardiovascular disease(CVD)in adults of Han people in Urumqi region.   Methods   Using group selected study, serum lipids, serum glucose, blood pressure, height, weight, waistline and hip girth of 2 688 people were measured.   Results   (1) There was significant difference between the means of serum lipids in the group of BMI≥18.5 and the group of BMI < 18.5(P < 0.01).There was significant difference between the means of serum lipids in the different groups in terms of waistline or WHR more or less than 70cm or 0.75 respectively.(2)There was significant difference in serum lipids between different blood pressure groups in which the blood pressure reading was more or less than 120/75 mmHg.(3)There was significant difference in serum lipids between the different groups in which fasting plasma glucose level more or less than 5.5 mmol/L or post prandial blood glucose level more or less 6.5 mmol/L.   Conclusion   There was strong relationships between serum lipids and the risk factors of CVD in adults of Han people in Urumqi region.
Investigation on epidemiology of overweight and obesity among adults in Liaoning province
Zhang Shu-juan, HOU Shu-wei, LIU Qi-nan,
2004, 20(4): 475-477.
Abstract(998) HTML (366) PDF 664KB(64)
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  Objective   To offer scientific bases aiming at controlling obesity, to investigate the epidemiology of overweight and obesity among adults in cities and suburban areas of Liaoning province.   Methods   Residents of 4 cities and their suburban areas, and staffs from two large enterprises are investigated by self-filled structured tables.WHO diagnosed standard for overweight and obesity and the age distribution of national census in 1990 were used to calculate the age-adjusted prevalence rates.   Results   Data collected from 37 070 adults show that age-adjusted prevalence rates of overweight and obesity are 24.77% and 2.87% respectively for males, significantly higher than the nation's average levels while 17.24% and 1.96% for females, significantly lower than that of nation's average.Prevalence rates of overweight for both males and females increase gradually with age, showing significant difference each other.Occupational characteristic of obesity in males shows that merchant and traffic transportation men have the highest rates, while unemployed and farmers have the lowest.As for females the rates of overweight in housewife and farmer are significantly higher than in other occupational groups.Education in females has a negative effect on rates of overweight and obesity, but shows opposite in male.   Conclusion   The age-adjusted prevalence rates of overweight and obesity of males are significantly higher in Liaoning than the nation's average levels.Age, sex, occupation, and education have significant impacts on these rates.
2004, 20(4): 477-477.
Abstract(625) HTML (164) PDF 122KB(25)
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2004, 20(4): 478-479.
Abstract(693) HTML (203) PDF 379KB(28)
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2004, 20(4): 480-480.
Abstract(520) HTML (195) PDF 114KB(27)
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2004, 20(4): 481-482.
Abstract(628) HTML (216) PDF 197KB(31)
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Effects of nutrients on alcoholic fatty liver in rats
GAO Jing-yu, GUO Jun-sheng
2004, 20(4): 482-483.
Abstract(703) HTML (274) PDF 477KB(24)
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  Objective   To examine the effects of complex nutrients on alcoholic fatty liver in rats.   Methods   High fat and ethanol induced alcoholic fatty liver models, and the intervention groups received nutrients before gavaging ethanol.After 4 weeks, all the rats were killed.The hepatic samples were taken for pathological section, and transaminases, lipids and lipid peroxidation were also estimated.   Results   The concentrations of serum AST、ALT and γ-GT, as well as TG, TC and FFA levels in intervention groups decreased significantly.Hepatic GSSG and LPO concentrations decreased severely in intervention groups also.   Conclusion   The intervention nutrients played an important role in preventing alcoholic fatty liver, and a possible mechanism was antioxidative capacity.
Analysis on resistance of strains producing extended-spectrum beta-lactamases
SUN JIie, YU Xiao-nan, FU Wei-xin,
2004, 20(4): 484-485.
Abstract(1056) HTML (326) PDF 425KB(93)
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  Objective   To analyse the resistance of strains producing ESBLs so as to give a better medicine therapy.   Methods   Strains produing ESBLs were identified by double-disk synergy test and their MIC were detected by agar dilusion test.   Results   The sensitivity of Imipenem was 85.8% and MIC was between 0.125-4.0μg/ml; Meropenem 94.0% and 0.125-4.0μg/ml.They had shown good antibiotic activity.Cefoperazone/Sulbactam 40.3%, MIC at 0.125-16.0μg/ml (44 strains); Piperacillin/tazobactam 56.0%, MIC 0.125-16.0μg/m(59 strains); Cefoxitin 47.0%, MIC 0.125-8.0 μg/ml(63 strains); Levofloxacin 61.9%, MIC 0.125-2.0μg/ml(83 strains); Ciproaoxacin 53.7%, MIC 0.125-1.0 μg/ml(72 strains); Amikacin 64.2%, MIC 0.125-8.0μg/ml(86 strains); Chloramphenicol, Cefotaxime, Cefepime and Aztreonam all below 40.0%.   Conclusions   Carbapenems had the best antibiotic activity.There were increases of intermediate resistane for Cefoperazone/sulbaetam and Piperacillin/tazobactam which have enzyme inhibitor.They have been overused in clinical may be the main reason.Quinolones have better antibiotic activity on bacteria except for Escherichia coli.
2004, 20(4): 486-486.
Abstract(544) HTML (142) PDF 126KB(35)
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2004, 20(4): 487-488.
Abstract(549) HTML (177) PDF 300KB(40)
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2004, 20(4): 488-488.
Abstract(701) HTML (508) PDF 230KB(33)
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2004, 20(4): 489-489.
Abstract(714) HTML (199) PDF 132KB(21)
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2004, 20(4): 490-490.
Abstract(685) HTML (222) PDF 223KB(88)
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2004, 20(4): 491-491.
Abstract(526) HTML (182) PDF 137KB(23)
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2004, 20(4): 492-494.
Abstract(801) HTML (249) PDF 756KB(51)
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2004, 20(4): 494-494.
Abstract(607) HTML (221) PDF 126KB(22)
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2004, 20(4): 495-497.
Abstract(695) HTML (227) PDF 755KB(36)
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Effect of different color paper and ink on visual fatigue
WANG Zhi-yong, QU Jia, ZHOU Xiang-tian,
2004, 20(4): 497-498.
Abstract(700) HTML (242) PDF 496KB(40)
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  Objective   To study the relation between myopia and luminance with chroma in paper and ink.   Methods   38 fifth-grade pupils were carried through the survey of subjective symptoms of visual fatigue during writing with three different color notebooks.Reflecting Index(RI)of notebooks was determined.Brightness Contrast(BC)was evaluated.   Results   (1) More subjects appeared visual fatigue as a result of using white notebooks.(2)RI of notebooks distributed in the range from 61.60% to 70.38%.RI of white notebook wasn't the highest than the others.(3)Writing with black or blue ink, BC distributed around 96%and 50%respectively.(4)Visual threshold(VT)of blue ink was 1.7 times larger than VT of the black.For the same sort of ink, VT at night was 1.3 times as in broad day.   Conclusion   (1) While being writtern, the yellow notebook resulted in less visual fatigue than the white, the yellow paper added tens of red dots produced less visual fatigue than the yellow paper.(2)Faint yellow notebooks was unable to decline the RI markedly than white was, but the darker yellow or the semi-gloss finish and matte finish papers were able to decrease it.(3)Using blue ink visual efficiency(VE)was lower than the black one.While writing at night, the VE of the same kind of ink was lower than in broad day.
Problem of community health service and strategy in Shenzhen
LI Xiao-hui, XIAO Ai-hua, ZHOU Xiao-qing
2004, 20(4): 499-500.
Abstract(775) HTML (207) PDF 430KB(104)
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  Objective   To study the main problems of the community health service in Shenzhen and provide the scientific basis for strategy making.   Methods   Both questionnaire and observation were adopted.   Results   The main problems existing in Shenzhen community health services were as follows: (1)the structural incoordination of the community health human resources, eg the doctor nurse ratio 1:0175, and low quality concerning the total health workers; (2)insufficient on-post training of the community health workers; (3)the expensive rent of the service office.   Conclusion   Following strategies were presented: (1)To make proper adjustment to the human resource structure of Shenzhen community health service; (2)To improve the community health service facilities along with the community development; (3)To create a training department for general medicine education under Shenzhen Medical Training Center, providing more chances to the community health workers for on-post training.
2004, 20(4): 501-502.
Abstract(619) HTML (174) PDF 1141KB(28)
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Risk analysis on HIV infection among drug users
ZHANG Ju-ying, GOU Li, XU Hong-chuan,
2004, 20(4): 502-503.
Abstract(946) HTML (246) PDF 351KB(32)
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  Objective   To explore the main risk factors for human immunodeficiency virus(HIV)infection in drug users, providing scientific bases for prevention and a reference method for similar study.   Methods   A modified Logistic regression by principal component was applied to explore the main risk factors for HIV infection among drug users.   Results   The significant risk factors related to HIV infection among drug users were type of drug-using, needles sharing, number of needle-sharing partners, regular needle-sharing partners, and handling of remaining blood in shared needles.   Conclusion   Advocating Methadone Maintenance and implementing needle-exchange plan and promoting safety injection were important measures to control HIV infection among drug users.
2004, 20(4): 504-505.
Abstract(826) HTML (276) PDF 1381KB(44)
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Study on relationship between body weight and waist circumference with prevalence rate of impaired glucose tolerance
XU Wei-min, ZHU Xiao-xia, JIN Xing-yi,
2004, 20(4): 505-506.
Abstract(889) HTML (204) PDF 1023KB(62)
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  Objective   In order to understand the relationship between body weight, waist circumference(WC), body overweight and obesity with the prevalence rate of IGT, to provide scientific information for IGT prevention and control.   Methods   Stratified cluster sampling method was applied, and totally 4 682 persons were randomly selected from two communities in urban area and two towns in suburban area.They were all older than 15 and lived there for more than 5 years.They did the questionnaire and accepted physical examination including their body height, weight, and WC.Their two-hour blood glucose after taking 75g oral glucose was also tested.   Results   The IGT prevalence rate of the older than 15 in the population in Hangzhou communities was 7.43% in males, 6.82% in females, and 7.07% in both sexes respectively.There was a trend that the IGT prevalence rate increased with the increase of body weight.But the IGT prevalence rate in the group with 30kg were higher than 40-60kg group.That present the for mrelation.The IGT prevalence rate of different WC group had a marked difference in statistic.To the WC group of 100 cm attain the high peak.This trend at male and female was consistent.The IGT prevalence rate of body overweight and obesity were significantly higher than that in the group with normal or light weight.OR values were 1.62 and 2.22 respectively.95% CI were 1.50-2.72 and 1.87-4.28 respectively.   Conclusion   The incidence of IGT is related to body over weight and obesity, especially to abdomen obesity.So remain proper body weight and normal somatotype was very important.In order to prevent the diabetes mellitus occurrence, intervention measures for fat and IGT population should be taken.
2004, 20(4): 507-508.
Abstract(512) HTML (148) PDF 112KB(26)
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2004, 20(4): 508-508.
Abstract(668) HTML (211) PDF 115KB(34)
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Study on supply and demand development for health manpower resources in Heilongjiang
MAO Jing-fu, LIU Xue-song, HAN Shu-jie,
2004, 20(4): 509-510.
Abstract(729) HTML (200) PDF 476KB(44)
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  Objective   To search characteristics for health manpower resources of Heilongjiang province and project situation of supply and demand about health manpower in 2012.To provide scientific evidence for developing plan in health manpower and medical education enacted by decision-making of government.   Methods   Efficiency demand and age shift calculating approach which belong to calculating method of health resources requirement recommended by WHO are adopted.Total investigation data for national health service in 1998 are used and predicted situation for supply and demand of health manpower of Heilongjiang province in 2012.   Results   By 2012, total demand of health technical staffs will be 18.85 ten thousand people.Practical possession of health technical staffs will be 23.25 ten thousand people.   Conclusion   Large possession、low quality and unbalance distribution of health technical staffs is typical nature of health manpower in Heilongjiang province.It is forecasted that supply of health manpower will be exceeding demand according to rising speed annually graduated form medical university of Heilongjiang province while other influencing factors keeping invariability.
2004, 20(4): 511-512.
Abstract(640) HTML (214) PDF 336KB(39)
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