2004 Vol. 20, No. 7
Display Method:
2004, 20(7): 769-771.
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Objective To express and purify the 56kDa protein of orientia tsutsugamushi (Ot) Gilliam strain in escherichia coli to obtain the abundant recombinant protein with high activity and to play a foundation for preparing the diagnosis kit of scrub typhus. Methods The gene (about 1 320 bp) encoding 56kDa protein of Ot was amplified by using PCR technique. PCR product was cloned into the PET 28a expression vector, plasmid PET-OTG was constructed and expressed successfully. The recombinant protein containing sixtag was purified by Ni2+ chromatography column. The positive fractions with significant amounts of protein were analyzed by SDS-PAGE and identified both by western-blot and indirect ELISA. Results The recombinant plasmid containing the aimgene was expressed successfully both by forming inclusion body and soluble protein, the expressed fusion protein was visualized on gel at molecular mass about 52 kDa. The inclusion body protein can get a higher purity than soluble protein after being purified by chromatography column. By western-blot the recombinant proteins can be recognized by patient's positive serum while the negative serum cannot resulted in the same blot band. By indirect ELISA, the recombinant proteins showed good antigenicity that can distinguish the positive and negative serum successfully. Conclusion The purified recombinant protein with immuno-reactivity may be a promising diagnostic antigen for preparing the diagnosis kit of tsutsug amushi disease.
2004, 20(7): 772-774.
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Objective To observe growth and development of the weaned mice fed with different levels of dietary zinc and to explore whether the expression of DMT 1 was induced by different dietary zinc intakes. Methods Twenty weaned mice(postnatal day 21)were randomly divided into four groups: zinc deficient (ZD), zinc adequate (ZA), zinc supplemental (ZS)and pair fed (PF)were fed with different levels of dietary zinc for 3 weeks(from postnatal day 21 to postnatal day 42).The zinc contents in the diet of ZD, ZA, ZS and PF were 1, 30, 180 and 180 mg/kg respectively. Form postnatal day 21 to postnatal day 42, the dietary intakes and wieght of the mice were measured every day. On postnatal day 42(P42), the mice were sacrificed. Tissues were immediately isolated and the serumzinc concentrations were measured by AAS. The expression of DMT 1 was determined by semiquantitative RT-PCR. Results The dietary intakes and weight of ZD mice were much lower than those of other groups. Serum zinc concentration in different groups changed significantly.DMT1 expression in test is declined significantly by higher zinc intake, but not in brain. Conclusion A dequate dietary zinc was very important to growth and development of weaned mice.DMT 1 expression of testis was significantly downregulated by supplemental dietary zinc, which was supposed as the protective feed-back mechanism to maintain incellular zinc homeostasis of weaned mice.
2004, 20(7): 775-776.
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Objective To study the influence of sphingosine on the growth of human colon HT-29 cells in vitro. Methods Using cell culture tests (MTT test, index of cellular mitosis, and 3H-TdR incorporation test), to examine the growth of HT-29 cells at five concentrations (0, 0.82, 1.63, 3.125, 6.25, 12.5μmol/L)with a negative control (DMSO v/v). Results Sphingosine can inhibited the growth of HT-29 cells. After treatment with various sophingosine concentrations mentioned above for 7 days, the inhibition rates were 18%, 45%, 72%, 87% and 89% respectively. With the increase of sphingosine concentrations, 3H-TdR incorporation was decreased in HT-29 cells and the quantity of 3H-TdR incorporation was significantly decreased as compared with the negative control (P < 0.05). Conclusion Sphingosine can significantly inhibit the growth of HT-29 cells, which was probably one of the preventive mechanisms of sphingomyelin for colon cancer.
2004, 20(7): 777-779.
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Objective To detect the alterations of k-ras gene and p15 gene and analyse the genomic instability in the malignant process of 16 HBE cells induced by crystalline nickel sulfide (NiS). Methods PCR-RFLP was used to detect the mutation of 12 code of k-ras gene and PCR-SSCP was used to examine the presence of alterations of k-ras gene exon 1 and p15 gene exon 2 in the malignant process of 16 HBE cells induced by crystalline nickel sulfide. Analysing the genomic instability by random amplified polymorohic DNA (RAPD). Results Compared with the negative control cells, alterations of p15 gene and k-ras gene were not observed and genomic instability was showed in the malignant process of 16 HBE cells induced by crystalline nickel sulfide. Conclusion Crystalline nickel sulfide could not induce alteration of p15 gene and k-ras gene, which indicated these four nickel compounds had not effect on the P15 gene exon2 and K-ras gene exonl, but they could induce genomic instability, furthermore, having special PCR bands, indicating genome became more instability in the malignant process.
2004, 20(7): 780-782.
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Objective In order to detect the gene homologies of orientia tsutsugamushi (Ot) isolated from scrub typhus patients, chigger mites, and rodents of Shandong province in epidemic season. Methods Nested-PCR connected with PCR/RFLP was used to identify the gene type of 12 strains Ot isolated from scrub typhus patients, the dominant chigger mite Leptotrombidium (L.) scutellare, and the dominant rodent Apodemus (A) agrarius in epidemic season of scrub typhus, 2 pools of homogenized L. scutellare, and 10 blood of scrub typhus, and compared with international reference strains Gilliam、Karp、Kato. Results Using PCR/RFLP, 24 had the similar restriction maps with Japan Kawasaki strain, but they had not restriction site of Hha Ⅰ, and thus had some difference in gene sequence compared with Japan Kawasaki strain. Using Nested-PCR, 24 had the same products in the amplification of template Ot-DNA, thus they all belonged to Kawasaki strains, which was endemic in Japan. Conclusion The gene types of Ot isolated from scrubty phuspatients, chigger mites, and rodents of Shandong province in epidemic season were identical, they all were similar to Japan Kawasaki strain, buthad some differences in gene sequence, and the transmission of Ot among patients, chigger mites, and rodents existed in Shandong province.
2004, 20(7): 784-785.
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Objective To study the effects of the nonvolatile organic compounds in tapwatr derived from Donghu Lake in Wuhan on DNA damage. Methods The nonvolatile organic compounds(NOCs)in water were abstracted with XAD-2 resin and the DNA damage of such NOCs in Hela cells were detected by using Signle Cell Gel Electrophoresis assay(SCGE). Results (1) Different orgainc extracts(50, 100, 200, 400 ml/25 ml)from all samples of tap water could cause DNA strand breaks in Hela cells at certain range of concentrations, and there was positive dose-effect relationship(P < 0.05);(2)A consistent potency for all the various hydrographic period, with decreasing activity in the order: average > low > high flow period. Conclusion The application of this assay would contribute to explain the mutagenic mechanism of the organic pollutants from tap water on the human body.
2004, 20(7): 786-787.
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Objective To study the change of the gene expression pattern of vascular endothelial cells induced by oxidized low density lipoprotein. Methods After human umbilical vein endothelial cells was exposed to oxidized low density lipoprotein(100 mg/L)for 6 hr, genes expression was screened with a complementary DNA microarray representing 4 000 different human genes and 96 control genes. Results There were 3 down-regulated gene and 5 up-regulated genes. Conclusion Ox-LDL could induce the change of gene expression pattern of endothelial cells. It was the first time to find that Ox-LDL could induce the change of the gene expression of H731, ST2 protein, Cyclin B1 and KDRF. These changes might be related to the dysfunction of endothelial cells induced by Ox-LDL.
2004, 20(7): 788-790.
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Objective To investigate the incidence of-thalassemia and the gene variations of beta-globin in Guizhou Congjiang Dong people. Methods To sample from venous blood and determine the HbF and HbA2 by the AM-electrophoresis to riddle the heterogotes of β-thalassemia, then the blood of heterogotes were analyzed by the auto matic blood cell analyzer, DNA extracted from white blood cells with the rountine method and analysed by PCR. and RDB (reverse dot blot) hybridization. Results Among the sampls, the incidence of-thalassemia carriers is 7.85%, The types of gene mutation in this area were CD41-42 (-TCTT) (9 cases, 23%) and CD17(A→T) (30 cases.77%). Conclusion The incidence of-thalassemia was very high in the area and the beta-globin gene variations were different to that of the reported Chinese population, which might be related to the intra-ethnic marriage, the conglomerate family mobility and the narrow marriage radius.
2004, 20(7): 791-793.
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Objective To screen differential expression genes between normal liver and hepatic fibrosis in rats and to demonstrate the molecular mechanism from gene. Methods mRNA differential display PCR (DD-PCR) was used. First both total RNAs were isolated and reversely transcribed into cDNAs respectively. The products of PCR were run on 6% denaturing polyacrylamide gels(PAG)and autoradiographs were examined to find the differential bands between both gels. The differential bands were reamplified using the same PCR condition as before. Reverse northern analysis was used to identify the bands. After sequencing, the DNA sequence was checked with GenBank data for homology. Results 16 differential bands were selected from the gel and 12 bands were special by repeated PCR. In the 4 sub-clones, 2 differentially expressed fragments were obtained(N2, F1)by reverse northern hybridization. Homology comparing, the sequence of N2 had 94% homology with Mus musculus apolipoprotein a5(apoa5), but F1 was not found. Conclusion By mRNA differential display, mRNAs from normal liver and hepatic fibrosis were compared to find one differentially expressed liver-fibrosis-related gene fragment. It will be effective to study the gene display to find the special curative method.
2004, 20(7): 794-795.
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Objective To investigate the damage of lead acetate and sodium arsenite on human immotalized keratinocytes line HaCaT and the protective effects of TFBP(total flzvonoids of broussonetia papyrifera)gotten from the leaves of broussoneria papyifera. Methods TFBP were gotten by use of the absorption chromatograph column of polyamide. MTT reduction assay were performed and the levels of malondialdehyde (MDA)were determined in cultured HaCaT cells. Human immotlaized keratinocytes HaCaT cells were irradiated by lead acetate and sodium arsenite radiation at different dosages. The protective effects of TFBP also were evaluated. Results With the different concentrations of 0.1-1.0 mmol/L lead acetate, the skin cells were markedly inhibited. Sodium arsenite at the lower concentrations of 0.5-1.0μmol/L induced mainly the degree of the proliferation of the HaCaT cells, while inhibiting cell growth over 5μmol/L.TFBP at the higher concentrations of 100 mg/L had certain protective effects from lead acetate or sodium arsenite, and the levels of MDA decreased, too. Conclusion Lead acetate and sodium arsenite could cause significant damage to HaCaT cells under the conditions of this study, TFBP gotten from the leaves of broussoneria papyifera had certain protective effects on HaCaT epithelial cells from those damage.
2004, 20(7): 796-797.
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Objective To study the correlation of polymorphism of CYP1A1Msp1 and seurm selenium level independently and in combination with the risk of lung cancer. Methods A case-control study was conducted, which included 58 cases of lung cancer and 62 controls. The genotypes of CYP1A1 were detected by PCR-RELP and the serum selenium level was determined by GHAFS. Results There was no significant difference in polymorphism frequencies of CYP1A1 Msp I between the two groups. The genotypes were no significantly associated with the risk of lung cancer; Average serum level of selenium among the lung cancer cases was significantly lower than that among controls(P=0.001);Comparing with those who had higher serum selenium(> 0.109 mg/L)with CYP1A1m1 m1, the odds ratios of those who had lower serum selenium(< 0.109 mg/L)with CYP1A1m2 m2, with CYP1A1m1m2, with CYP1A1m1 m1 were 9.00(P=0.006), 3194(P=01195), 5.40(P=0.036)respectively, and those who had higher serum selenium with CYP1A1 m2m2, with CYP1A1m1 m2 were 1.69(P=0.500), 1.13(P=0.705)respectively. Conclusion Polymorphism of CYP1A1 was not associated with the risk of lung cancer, but there was significant inverse association between the serum selenium level and the risk of lung cancer. A combined effect of polymorphism of CYP1A1 Msp I and serum selenium level on the risk of lung cancer was suggested.
2004, 20(7): 798-799.
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Objective To elucidate the effects of early intervention on intelligence development of infant twins. Methods 88 infant twins(triplets)born during Sep.2000-Aug.2002 were assigned to three groups: early intervention group(A)41 cases; comparision group(B)47 cases; normal comparison group(C)51 cases. When infants group A born for one month, early intervention was given to them. All the infants in the above three groups received infant development test of Child Developmentd Centre of China (CDCC) when they were at the corrected age of one year old. Results Average scores of mental development index(MDI)and psychomotor development index(PDI)in group A were 8.9 and 7.0, respectively, higher than those in group B, there were significant difference between A and B groups.(P < 0.01, 0.05).Both MDI and PDI scores in group A caught up with those in group C; but in B, the scores were 13.7 and 8.4, respectively, lower than those in group C, there were significant difference between B and C group.(P < 0.001, 0.05).In height and head circumference there were no significant difference between A, B and C groups. In weight, A nd B were lower than those in group C infants(P < 0.01, 0.05). Conclusion Early intervention could promote intellectual development of infant twins.
2004, 20(7): 799-800.
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Objective To probe the risk factor and routes of transmission of SARS in Guangzhou. Methods 236 SARS IgG positive cases and 206 negative cases in Guangzhou were selected and the logistic regression was adopted to analyse the related factors. Results Healthcare workers and multiple persons clustered in the same office or worksplace were apt to infected the SARS virus OR=9.63, 95% CI (3.67-25.26)and OR=4.05, 95% CI (1.34-12.20), respectively; Having not went to hospital during the epidemic period and non-associated with animal were less likely to infect the SARS virus, OR=0.39, 95% CI (0.18-0.83)and OR=0.29, 95% CI (0.14-0.61), respectively; Having not injected the bacterin of measles and flu and the smokers were impressionable to the SARS virsus, though the possibility was not significant, OR=2.03, 95% CI (0.93-4.41) and OR=2.43.95% CI (0.79-7.44), respectively. Conclusion Persons clustered in the same office or worksplace and badness aeration were the risk factors of infected SARS besides the closely contact with SARS patients. The history of animal contact was significantly correlated to the SARSIgG, which suggested that the SARS virus likely originated from the animals.
2004, 20(7): 801-803.
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Objective To investigate effect of fluoride on the ultrastructure of rat lumbar vertebrae cancellous bone. Methods Sodium fluoride was administered to female Wistar rats through drinking water with three doses of 25, 50 and 100 mg F-/L for a period of 3 months. Cancellous bone from the second lumbar vertebrae was studied morphologically with a scanning electron microscope. Results The significant structural changes in bone trabecula and collegen fiber were observed in the fluoride-treated animals as compared with normal bone. With the increase of fluoride dose being increased, bone trabecula numbers were increased, the wall of bone trabecula was thickening, and the surface of bone trabecula was rough and uneven. The collegen fibers showed sclerosis-looking changes. Some wide crevices in bone trabecula could be observed under the scanning electron microscope. Conclusion Long-time exposure to high fluoride may cause more bone formation in rat lumbar vertebrae cancellous bone, but the new-formed bones were not well arranged. There were bone trabecula cracks and tiny fracture phenomena of separated quality and volume of bone trabecula in rat lumbar vertebrae cancellous bone could be seen.
2004, 20(7): 804-805.
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Objective To study the role of the pharmaceutical metabolism enzyme during the inhibitory effect of VES against B (a) P in mice. Methods The model of B (a) P-induced forestomach cancer in mice would be established firstly to check whether there was any difference between the two kinds of demonstrating ways. Then to select the best way to do the following experiment. The activities of EROD and GST in liver S-9 fraction were measured by spectrophoto fluorometer and GST kit, respectively. Results VES both in oral and ip. can decrease the development of B (a) P-induced forestomach cancer in mice. While the activity of EROD, phase ⅳ pharmacological metabolism enzyme, in the VES group(39.1±3.05), were decreased significantly(P < 0.01), compared with the B (a) P group (57.9±3.16). Conclusion VES can decrease the carcinogenesis of forestomach cancer in mice involving the depressing activities of EROD.
2004, 20(7): 807-808.
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Objective To explore effective methods by estimating the size of clients of female sex workers in present China. Methods Contacted subjects by several approaches including female sex workers volunteers, STD clinics, long-distance bus stat ions, building site, long-distance freighting station and adopting nomination methods, and applied multiplier method to estimate the size of clients of female sex workers in Leshan downtown district. Results The size of clients of female sex workers calculated in Leshan downtown district was 25 926-29 863 and frequency of sexual intercourse was 15.39 per year. Conclusion The ways to contact subjects effectively and to ensure sampler epresentative were sex workers volunteers, longdistance bus stations and adopting snowballing methods. Applying multiplier method could calculate the size of the clients simply and quickly.
2004, 20(7): 808-809.
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Objective To identify live attenuated hepatitis A (HAV) vaccine virus seed and detect adventitious agents, monoclonal antibody is used to neutralize instead of human and rhesus monkey immune serum. Methods Choosing suitable monoclonal antibody concentration to neutralize HAV for confirming the neutralization capacity of monoclonal antibody. To test adventitious agents after monoclonal antibody neutralized HAV vaccine virus seed, and further apply to identification test. Results The monoclonal antibody (1:100) could neutralize 105 CCID50/ml HAV virus and it was 100 times than that of human or rhesus monkey immune serum; Applied that high efficiency monoclonal antibody to hemadsorption test and non-hemadsorption test for adventitious agents, the results were negative and the monoclonal antibody could be used in identification test. Conclusion With high-efficiency monoclonal antibody on adventitious agents detection, it can be applied a small scale dilution of HAV virus seed to aviod the difficult of testing adventitious agents by large scale dilution of vaccine virus seed. The monoclonal antibody was used to neutralize instead of human and rhesus monkey immune serum.
2004, 20(7): 810-812.
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Objective To estimate relative risk between body mass index and diabetes. Methods To collect cross-sectional studies on the relative risk between body mass index and diabetes for 1990 to 2002, 18 studies finally was involved into meta-analysis after quality evaluation. Results Adults of relative risk of BMI≥24 or BMI≥25 are 2.28 and 2.46 by fixed effect model analysis. Conclusion High BMI is one of important risk factors for diabetes.
2004, 20(7): 813-814.
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Objective To realize the transformation shape of influenza virus under the action of split, and the action about the virus substructure shape. To learn split way. Methods To draw split influenza viruse specimen of A1, A3 and B subtypes at different time, and observe under electron microscope. The split solvent is Triton N-101. Results The splitting of influenza virus can be divided into four stages. The stage includ M protein (Mp) membrane swell neuraminidase (NA) hemagglutinin (HA) lipid bilayer (LB) break away phase, Mprotein (Mp) membrane disrupt, nucleocapsid (NP) spill and Mprotein dissolved phase. Conclusion The method of vibration makes split better.
2004, 20(7): 815-817.
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Objective To investigate the change of expression of MTAP gene and its linked genes p14, p15 and p16 in patients with non-small cell lung cancer and its clinical significance. Methods Thirty fresh samples of local cancer tissue and its nearby tissue from 15 patients with lung cancer were analyzed for mRNA of MTAP gene and its linked genes p14, p15 and p16 by RT-PCR. Results In 15 tumor samples, 11 (73.3%) were not detected the MTPA expression or the transription level of MTPA mRNA was reduced by more than 5 times; but in nearby tisue samples, the rate of MTPA expression was as high as 93.3%.In addition, the low level of expression in samples from lung adenocarcinoma and from squamous cell carcinoma did not different significantly. But the low expression rate of MTPA in intermediate and poorly differentiated lung cancer was significantly higher than that in well-differentiated cancer. Furthermore, RT-PCR was performed to observe the differentiated cancer. Furthermore, RT-PCR was performed to observe the differences between MTAP gene and its linked genes p14、p15 and p16 for 7 patients with lung cancer. Unexpectedly, it was found that p16 and p14 were expressed athigher level in 3 of 7 tumor samples respectively than in matched normal tissues, while p15 mRNA was deficient in 5 tumor samples. Conclusion The low expression or loss of MTPA gene may be relevant closely to the differentiation degree in lung cancer. The loss of MTPA gene may be independent of p16 gene, which suggested that the low expression or loss of MTPA should be of special biological significant in tumor biology.
2004, 20(7): 817-817.
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Objective To study method of GTF abstracted from chromium-rich brewer's yeast, determine content of amino acid in abstract. Methods Extraction and ultrafilitraction were used to obtain abstact from chromium-rich brewer's yeast. Results Many kinds of amino acids were determined in abstract. Content of glutamine, glycine and cystine was high (3878, 670, and 424 mg/L)。 Conclusion Concentration abstrated by eutraction and ultrafitration contained lots of amino acids which were the content of GTF.
2004, 20(7): 818-819.
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Objective To grasp the depression status of women in the life of menopause for developing mental health education and producing health care measures. Methods Using questionnaire SDS to interview 105 women in the life of menopause, and select 105 men in the life of menopause as control group. Results The depression level of women in the life of menopause was higher than that of men in the life of menopause. Conclusion There was significant difference between man and woman not only in physical, but also in psycho logical, so the woman had tendency to develop endocrinopathy and psychosomatic disorder. Some influencing factors such as marital status, family stauts, insomnia, physical disease, economical status and inter personal relationship were as marital status, family stauts, insomnia, physical disease, economical status and interpersonal relationship were important. It was no doubt to provide necessary psycho logical intervention for the woman in the life of menopause.
2004, 20(7): 821-822.
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Objective To observe the effect of static magnetic field (SMF) on the spinal cord neurons in Vitro. Methods Spinal cord neurons were treated with different intension of static magnetic field and the growth of the cells was analyzed by plotting cell growth curve and MTT assay. The growth condition of cell was observed with phase-contract microscope, and intracelluer dehydrase activity was detected. Results 1-10 mT of static magnetic field had no inhibitory effect on the spinal cordneurons. The effect developed when the intension was up to 50-200 mT, and the inhibitory effect of magnetic field became more significant with the increase of the intension. Conclusion Hint to certain strength of static magnetic field had inhibitory effect on spinal cord neurons in vitro, there was dose-effect relationship intension.
2004, 20(7): 823-824.
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Objective To understand the purity and the impurity of the synthesized ebselen (Ebs) and the acute toxicity. Methods The Ebs was tested with HPLC, the impurity was detected with chromatometry and the acute test was done by adultratlav aged one time and the 24-hour mortality rate was observed. Results The purity of Ebs was 99.95%-99.99%, the amount of phenylamine was 0.000 17%-0.000 19%, the LD50 of the rat lavaged by mouth one time was 2 355.05mg/kg. Conclusion The quality of Ebs was reliable, the security was high, and it could be used for exploitation and study.
2004, 20(7): 825-826.
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Objective To investigate the drug-resistance of gram-positive cocciislated from patients in the First Affiliated Hospital, China Medical University. Methods Antimicrobial susceptibility of 2104 clinical isolated from 1999-2003 was tested by K-B method and Broth microdilution method. Results Of 2104 clinical isolates, staphylococcus aureus accounted for 20.5%, coagulase-negative staphylococci 42.8%, enterococci 32.9%.The detectable rates of methicillin-resistant staphylococcus aureus and methicillin-resistant coagulase-negative staphylococci in 2003 were 19.4% and 81.1%, which were higher than those in 1999 (17.1% and 65.4%). The difference in methicillin-resistant coagulase-negative staphylococci was significantly during 1999-2003 (P < 0.05).Neither strains of staphylococcus aureus norstrains of coagulase-negative staphylococci were found resistant to vancomycin.4 strains of coagulase-negative staphylococci showed resistance to teicoplanin. No significant changes in vancomycin-resistant entrococci were found from 1999 to 2003.The prevalence of vancomycin-resistant enterococci (1999-2003) were 9.8%, 7.7%, 5.0%, 4.1%, and 6.7% respectively.67.1-76.7 percent of E. faecalis isolates were sensitive to ampicillin, but ampicillin-resistant E. faecium were higher (66.7%-89.7%). The prevalence of teicoplaninr-esistant enterococci was 2.1%, 2.5% and 5.5% respectively. Conclusion Multidrug-resistant gram-positive cocci including methicillin-resistant staphylococcus aureus and methicillin-resistant coag ulasenegative staphylococci and ampicillin-resistant E. faecium are increasing.
2004, 20(7): 830-831.
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Objective To study the toxic effect of nickel sulfate on the germ cells in female rats and its mechanism. Methods Nickel sulfate was injected intraperitoneally for Wistar female rats with sexual maturation daily at different doses (1.25, 2.50, 5.00 mg/kg) for 21 days. The number of superovulation and surviving ovum were observed through cell culture, Activity of nitric oxide synthase (NOS) and nitric oxide (NO) content in the ovary were measured. Results The number of superovulation and surviving ovum was lower than those in the control group; activity of NOS and NO content in the ovary of the rats given nickel sulfate were higher compared with control group. Conclusion The ovary of the female rats was damaged by peritoneal injection of nickel sulfate, the number of superovulation and surviving ovum decrease was possibly associated with the increase of activity of NOS and NO content in the ovary.
2004, 20(7): 832-833.
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Objective To develop sandwich immune-PCR system for monitoring antigen. Methods Avidin was used to bridge the biotinylated antibodies and biotinylated DNA, and to detect antigen by immuno-PCR. Results As little as 0.1ng/L of SEB could be detected by the sandwich immuno-PCR, which was 103 times more sensitive than ELISA parallel control. Conclusion The universal immuno-PCR system can be used to detect ultra-micro antigen.
2004, 20(7): 837-839.
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Objective To evaluate the performance of All Population Tuberculosis Control Project of the World Bank Loan for 8 years and discuss the method of evaluation in project's performance. Methods Data were collected from 1993 to 2001 for transfer and acception of suspected patients, the smear-positive, the smear-negative of sputum TB, cure, raise and usage of the fund. Epidemic investigation on pulmonary TB and drug resistance monitoring was completed. The evaluation was done from the aspect of service equity, health accessibility, project effect, project benefit and project efficiency. Results (1) service equity: The external population was bring into the project management and the strategy of all population control was done, the effect was the same as population with residence card's; (2)health accessibility: three-level prevention and control network of city, area and town was perfected, and take measure of DOTS strategy; (3)effect evident: The project result in reducing 1.5×104 incidence and dropping beyond 50% in prevalence rate, multiple drug resistance rate in initial smear-positive dropping 7%.(4)benefit great: its direct economic returns were ¥1.42×109 and its indirect economic returns were redeem GDP ¥30.6×109; it retrieve individual income for tuberculosis ¥17.6×109.(5)efficiency high: the sputum conversion rate was 88.8% in the end of February and was 96.0% in the end of March. Conclusion It was developing great social effects and economic returns since the project was done for 8 years and it was essential for government promise to develop the project continously.
2004, 20(7): 843-845.
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Objective To investigate the seroprevalence, epidemiological characteristics of hepatitis E virus (HEV) infection in rural area of Zhenjiang provine and explore the association between breeding livestocks and hepatitis E virus infection. Methods Cluster sampling was used in norther nrural area of Zhejiang province in 2003.In a door to door survey, 850 healthy persons aged over 7 years were interviewed. Anti-HEV IgG and IgM were tested by commercial ELISA kits. Correlated risk factors were studied. Results The anti-HEV IgG and IgM prevalence were 46.35% (394/850) and 3.06% (26/850), respectively in the general population. The seropositivity rates of anti-HEV IgG in male and female were 53.62% and 39.45% respectively (P < 0.001) and the anti-HEV IgM were 1.45% and 4.59% respectively (P < 0.01). The antibodies increased with advancing age, but remain a stable level from the age of 45, the seropositivity rates of aged 25 and > 25 were 15.22% and 52.88% respectively (P < 0.001). Conclusion The prevalence of hepatitis E virus infection in this district was higher than any other reported districts in China. HEV infection was significantly associated with gender and age. Males were easily to contact hepatitis E virus. The prevalence of heaptitis E increased with age, but remained a stable level from certain age. No statistical association was met between HEV infection and the risk factors was assessed.
2004, 20(7): 852-853.
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Objective To analyze the epidemiological features of measles and surveillance results since establishing the surveillance systen in 1999, try to acquire scientific gist for measles control and elimination. Methods To analyze the epidemiological data and surveillant data during 20 years. Results The average incidence rate of the measles was 6.14 over 100 000 every year in 1984-2003.At the beginning of the district foundation, the average incidence rate of the measles was 24.42 over 100 000, at a high level. After 1987, the incidence rate decreased, but in 1992, 2001, the incidence rate rose up remarkably, got to 12.56 and 21.70 over 100 000 respectively, and measles outbreak occur red with periodic attack. The proportion of the age under 15 years old was 70.42%, but ten years lastly, the proportion of adult (> 15 years old) was growing from 10.50% (1984-1993) to 42.06% (1994-2003). Male accounted for 57.96%.The ratio of temporary residents and permanent residents was 3.18B 1.The cases with immunization blank or unsure occupied 82.54%.Seasonal peak of measles appeared in March to August (69.55%). Conclusion Since the district foundation the incidence rate of measles dropped considerably, but it appeared periodic and outbreak in small place. The ages of the measles cases were older than before, the model of the age with measles became mixed age rather than young age. The methods to enhance preventive inoculation for transient children, adults in danger, increase vaccine coverage rate, reinforce surveillance and prevent outbreak were our emphasis from now on.
2004, 20(7): 867-869.
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Objective To analyse the correlation between polymorphism of the angiotensiogen gene, environment factors, and essential blood hyper tension. Methods To observe the blood pressure of the patients with essential hypertension patients in west of Anhui. Polymerase chain reaction (PCR) combined with restrictive fragment length polymorphism (RFLP) were used to detect genetype frequency and polymorphism for angiotensiogen gene (A-20C). Results The frequency of allele C and A accounted for 15.3% and 84.7% in the total populations. Through chi-square test, it meeted with H-W balance (χ2=0.58, P > 0.05). The linear regession of AGT genetype type with the baseline blood pressure value showed that SBP of the mutation type (AC+CC) group were higher than AA group (P < 0.05), especially in female through linear Logistic regression (P < 0.05). Conclusion It was the first time in China to report the AGT (A-20C) gene polymorphism and genetype frequency. High blood pressure was chiefly show ed SBP increase for the total population with mutation type (AC+CC), especially among female.
2004, 20(7): 883-884.
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Objective To understand the psycological needs of HIV/AIDS. Methods Questionnaire and in-depth in2 terview methods were used to survey psychological needs of 111 HIV/AIDS. Results Most of patients with HIV positive felt disbelive and afraid and didn't tell someone else after infection. The proportion of desiring to communicate with other infectioners, among the ones transmitted by drug and donon blood were 75.56% and 87.10% respectively, of which 94.29% and 100% took face-to-face communication as first choice. Conclusion HIV/AIDS caused heavy stress. An effecive measure should be taken to improve the situation.
2004, 20(7): 887-888.
Abstract:
Objective To construct a recombinant plasmid which contained fragments of HEVORF2 and ORF3 chimeric gene, and then assess its feasibility for use as a DNA vaccine. Methods HEVORF2 fragment and full-length ORF2 was amplified by PCR.The PCR products were inserted into an eucaryotic expression plasmid pcDNA 3 to form a recombinant plasmid named pcHEV23(DNA vaccine).BALB/c mice were immunized with DNA vaccine and the humoral and cellular immune responses of the mice were detected. Results The HEVDNA vaccine can induce specific anti-HEV IgG and T lymphocyte proliferation. The lymphocyte stimulation index(SI)in the group immunized with pcHEV23 was high than that in control group(P < 0.01). Conclusion HEV DNA vaccine constructed by us can successfully induce both humoral and cellular immune response. Thus providing an experimental evidence for its use in control of HEV infection.