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2005 Vol. 21, No. 6

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Relationship between renin-angiotensin system activity and essential hypertension in Mongolian people
WANG Yanhua, TONG Weijun, LIU Yongyue,
2005, 21(6): 641-643. doi: 10.11847/zgggws2005-21-06-01
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  Objective   To explore the association between activity of renin angiotensin system and essential hypertension in Mongolian people in China.   Methods   Based on a previous survey, 450 hypertensives and 590 nor motensives were recruited as study.They were divided into three pedigree groups according to genealogy of hypertension, i.e., hypertensive, non hypertensive and unknown ones, and each group subdivided into hypertensive cases & normal controls.Radioimmunoassay(RIA) was utilized to measure levels of angiotensin(Ang), angiotensin(Ang)and plasma rennin activity(PRA), respectively.   Results   There was significant difference in plasma level of Ang and Ang and PRA in three pedigree groups(P < 0.001).Not only significant difference in levels of Ang and Ang between the hypertensives and nor motensives in three pedigree(P < 0.05)was observed, but also significant difference in Agn and Ang was observed between the hyperten sives in hypertensive pedigree and the normotensives in non-hypertensive pedigree, respectively(P < 0.05).There was significant difference only in plasma level of PRA between the hypertensive and normotensive group of hypertensive genealogy(P < 0.05).   Conclusion   Plasma level of Ang, Ang and PRA may be related to hypertension in Mongolian people, and PRA seems to be correlated with family history hypertension.
Study on rate of awareness and effective factors of hyperension in rural population of Hebei
WANG Xiaobo, JIANG Hong, WANG Liqin,
2005, 21(6): 643-644. doi: 10.11847/zgggws2005-21-06-02
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  Objective   To investigate the awareness rate of hypertension and main effective factors, and to provide the foundation for the intervention hypertension program rural areas in Hebei province.   Methods   Analyzed data were from stratified sampling survey in Hebei rural population aged over 15 years old in 2002.The unconditional logistic regression was used to study main effective factors on hypertension.   Results   The overall of the awareness rates were 26.1% in rural Hebei province, and in men and women were 22.7%, 29.4%, respectively.The multivariate logistic regression analysis showed that the main effect factors on the rate of awareness were age, blood pressure measured; hypertension history; body mass index, and sex.The OR value were 1.062, 1.670, 3.183, 1.090 and 1.802, respectively.   Conclusion   The awareness rate of hypertension was low in Hebei rural province.The comprehensive intervention measure should be taken to increase awareness rates of hypertension; to prevent and to control the occurrence of hypertension.
Analysis on hypertension prevalence status and its risk factors in Henan province
YANG Yongli, SHI Xuezhong, XIE Jing
2005, 21(6): 645-646. doi: 10.11847/zgggws2005-21-06-03
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  Objective   To know prevalence status and risk factors of hyper tension of people over 18 in Henan province.   Methods   Using the method of multistage stratified sampling, 3 000 people were investig ated with health and health system responsiveness.The questionnaire was designed by WHO.   Results   The prevalence incidence of hypertension in Henan province was 14.63%.The standard prevalence incidence was 17.15% basing on the data of the fifth population overall survey in Henan.The prevalence incidence of hypertension had positive relationship with age and drinking frequency, γs was 0.01 and 0.088, but had negative relationships with diploma degree, γs=-0.013.In different area, drinking frequency, diploma degree, BMI, sensitivities factors, the prevalence incidence of hypertension was different.   Conclusion   The prevalence incidence of hypertension in Henan was lower than that in countryside.Giving health education to the population of middle old age, drinking, lower diploma degree, fator overweighted, lower spirit played an important role on the prevention of hypertension.
2005, 21(6): 646-646. doi: 10.11847/zgggws2005-21-06-04
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Study on hypertension and its risk factors among drivers
SHUAI Ping, LU Xiane, JIA Qingliang
2005, 21(6): 647-649. doi: 10.11847/zgggws2005-21-06-05
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  Objective   To investigate the prevalence of hypertension and its risk factors among drivers and to developreasonable preventive strategies.   Methods   An epidemiological investigation including physical exams and blood pressure were conducted among 746 male drivers aged 20 to 60 in Chongqing.so did 884 male people who were not drivers.   Results   The standardized prevalence rate of hypertension among male drivers was 28.39%.It was higher than that of the male population who were not drivers in the same area, which was only 18.26%.Years of driving, working hours per day, different types of vehicles, job task and job strain were related with hypertension among drivers.Logistic regression showed that years of driving, job task, job strain, parents hypertension history and BMI > 25 were the main risk factors.   Conclusion   Job stress and other factors were related with the hypertension among male drivers which needed corresponding prevention and treatment.
Epidemiological research on blood pressure in 5762 children of Beipiao city
OU Fengrong, SHI Jingpu, GAO Jianing,
2005, 21(6): 649-651. doi: 10.11847/zgggws2005-21-06-06
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  Objective   To appro ach the status of children's blood pressure and distribution features of children's hypertension.   Methods   5 762 children aged 7-14 were sampled by means of whole population random sampling.Blood pressure was measured and some related factors were investigated with the uniform questionnaire.Spss 10.0 of statistical software was used for data analysis.   Results   (1) No matter of SBP or DBP, there was a significant difference between girls and boys, mean blood pressure level in girls was obviously higher than that in boys.Mean values of SBP and DBP were both increased with ag ing.(2)Age-BP curves of boys and girls showed different shape among different regions.In urban group, the two curves of hypertension was 2.8%, significant difference was found in prevalcence rate between girls and boys.The same difference was al so demonstrated between city and the countryside.Significant difference was also showed among five areas in suburb, while the prevalence rate in western area was significantly lower than that of other areas.   Conclusion   The area of Beipiao City was a high incidence region of child hypertension.Age-BP curves of boys and girls showed different shape among different regions.
2005, 21(6): 652-653. doi: 10.11847/zgggws2005-21-06-07
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2005, 21(6): 653-653. doi: 10.11847/zgggws2005-21-06-08
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2005, 21(6): 654-655. doi: 10.11847/zgggws2005-21-06-09
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2005, 21(6): 655-655. doi: 10.11847/zgggws2005-21-06-10
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Effects of EGF and genistein on proliferation of human breast cancer cell
WANG Li, LI Zhong, MO Baoqing
2005, 21(6): 656-657. doi: 10.11847/zgggws2005-21-06-11
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  Objective   To observe the effect of genistein and epidermal growth factor(EGF)on the proliferation of human breast cancer cell MCF-7 in vitro.   Methods   Cell growth status was observed with methyl thiazolyl tetrazolium (MTT)when it was cultured with different doses of genistein or EGF for different time respectively, or with definite dose of genistein and EGF.   Results   The growth of MCF-7 was inhibited when it was exposed to genistein over 25 μmol/L seemed to be an optional dose.Cell growth was greatly promoted when it was exposed to EGF over 4.15 nmol/L, the more the dose and time became, the greater the promoting effect was, and 8.30 nmol/L seemed to be an optional dose.When MCF-7 was cultured with 50 μmol/L genistein and 8.30 nmol/L EGF simultaneously, an interaction effect was observed.The promoting effect of EGF was reversed by genistein.   Conclusion   Genistein can inhibit the proliferation of human breast Cancer cell MCF-7 induced by EGF.
Relationship between genetic polymorphisms of N-acetyltransferase 2 and susceptibility of lung cancer
LIANG Geyu, PU Yuepu, YIN Lihong
2005, 21(6): 658-660. doi: 10.11847/zgggws2005-21-06-12
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  Objective   To investigate the relationship between N-acetyltransferase 2(NAT2)genetic polymorphisms and lung cancer susceptibility.   Methods   A 1:1 matched case-control study was performed in which 215 hospital controls were matched to the 215 original lung cancer cases.M1, M2 and M3 alleles of NAT2 were detected by PCR-RFLP technique.The association between NAT2 polymorphisms and lung cancer risk was analyzed.   Results   The distribution of M3 allele between lung squamous carcinoma cases and controls was significantly different(χ2=4.30, P < 0.05).M3 allele was related with increased risk of lung squamous carcinoma(OR=1.63, 95%CI=1.03~2.59), especially in nonsmoking population(OR=2.23, 95%CI=1.07~4.65).No significant association was observed between NAT2 acetylator genotypes and lung cancer risk(P > 0.05).   Conclusion   M3 > allele of NAT2 may be associated with susceptbility of lung squamous carcinoma in nonsmo king population.
2005, 21(6): 660-660. doi: 10.11847/zgggws2005-21-06-13
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Study on C667T gene polymorphism and susceptibility to gastric cancer
BI Jianping, CAI Lin, ZHENG Zongli
2005, 21(6): 661-663. doi: 10.11847/zgggws2005-21-06-14
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  Objective   To study the association between genetic polymorphisms of MTHFRC677T and susceptibility to gastric cardia/non cardia cancer, and to investigate the interaction of gene and intake of folates and alcohol drinking in etiology of these cancers in Fujian population.   Methods   The case control study with the same control group was conducted.The gene types were investigated by PCR-RFLP.   Results   The variated genotype(CT or TT)of MTHFR increased the risk of non-cardia gastric cancer after adjusting for potential confounders.There were interactions between variated genotype and low intake of fresh vegetables, fresh fruits and high drinking of alcohol in cardia cancer group.While the interactions between variated genotype and low intake of pean products, fresh fruits were observed in non-cardia gastric cancer group.   Conclusion   The synergies between MTHFR variated genotype and low level of folates intake, high alcohol drinking increase the risk of cardia cancer.The variated genotype is a risk factor of non-cardia gastric cancer.The synergies between genotype and low level of folates intake increase the risk of non-cardia gastric cancer.There maybe different risk factors between cardia and non-cardia gastric cancer.
2005, 21(6): 663-663. doi: 10.11847/zgggws2005-21-06-15
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Effects of genetic polymorphism of cytochrome P4502 E1 on susceptibility to gastric cancer
WANG Yu, JIANG Youhong, SUN Wenjuan
2005, 21(6): 664-665. doi: 10.11847/zgggws2005-21-06-16
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  Objective   To investigate the relationship between genetic polymorphisms of cytochrome P450 2E1 (CYP2E1)and the susceptibility to gastric carcinoma, and to study the action between environmental and genetic factors in the development of gastric carcinoma.   Methods   The Rsa Ⅰ poly mophism of CYP2E1 of 48 patients with gastric cancer and 48 controls were detected by PCR-RFLP.Related information of environmental factors were collected and analyzed by using the multiple-variable Logistic regression models.   Results   The frequency distribution of 3 genotype of CYP2E1 were 68.8%, 29.2% and 2.1% in cases and 45.83%, 47.92% and 6.25% in controls(χ2=5.389, P=0.068).The frequencies of the allelec1 and c2CYP2E1 were 83.3%, 16.7% respectively in cases and 69.8%, 30.2% respectively in controls.Gastric cases were more likely to carry allelec1 than controls.(χ2=5.241, P < 0.05).Multi variable stepwise logistic regression showed thathigh intake of salt, sausage and fried food were the risk factors for gastric cancer, while consumption of tea was the protective factors for gastric cancer.   Conclusion   Genetic polymorphism of cytochrome P4502 E1 and some environmental factors were significantly related with the risk of gastric cancer.
Effects of anthocyanin on matrix metalloproteinase expression in human umbilical vein endothelial cell
XIA Min, ZHU Huilian, TANG Zhihong,
2005, 21(6): 666-668. doi: 10.11847/zgggws2005-21-06-17
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  Objective   To explore the effect of ant hocyanin on matrix metalloproteinases(MMP)expression and activity.   Methods   Human umbilical vein endot helial cell were isolated and incubated with 5μg/mL rCD40L for 24 h.After that, the endot helial cell were incubated with 1μM, 10μM, 100μM C3G and P3G for another 24 h, respectively.   Methods   of zymography and Western blotting were used to analyze MMP-1, MMP-2, MMP-9 activities and protein expression.   Results   Ant hocyanin C3G and P3G inhibited MMP-1, MMP-2 and MMP-9 protein expression and their activities significantly.   Conclusion   Ant hocyanin C3G and P3G inhibited MMP-1, MMP-2, MMP-9 protein expression and their activities.
2005, 21(6): 668-668. doi: 10.11847/zgggws2005-21-06-18
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Effect of lovastatin on cell growth of human promylocytic leukemia cell lines
ZHANG Xueling, MI Mantian, LI Dengsong,
2005, 21(6): 669-670. doi: 10.11847/zgggws2005-21-06-19
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Study on a high throughput method of screening adducted site of DNA adducts
FENG Shuidong, ZHANG Hongxia, CHEN Feng,
2005, 21(6): 671-672. doi: 10.11847/zgggws2005-21-06-20
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  Objective   To develop a high throughput method of screening the binding site of DNA adducts.   Methods   The human liver tumor HepG2 cells were first treated with lng/μl nitrogen mustard for 3h and total DNA was extracted, then serrial analysis of gene expression was used for reference to improve ligation mediated PCR to detect the adducted site by nitrogen mustard in the cell genome.   Results   PCR product corresponded to 75 base pairs approximately.   Conclusion   The design of the experiment is rational and feasible.
Relationship between quality of life and stigma among people living with HIV/AIDS
YANG Yi, ZHANG Konglai, LI Zerong,
2005, 21(6): 675-676. doi: 10.11847/zgggws2005-21-06-22
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  Objective   To explore the relationship between quality of life and stigma among persons with HIV infection or AIDS.   Methods   Anonymous, self-administrated surveys were completed by 215 people living with HIV/AIDS, by using WHOQOL-BREF(Chinese version)and Berger-HIV Stigma Scale to measure quality of life and perceived stigma.Canonical correlation analysis was used to analyze the relationship between quality of life and stigma.   Results   The average scores of four domains were 50.74(physical), 46.01(psychological), 53.78(social relationships)and 43.94(environment)for quality of life, and 49.89(personalized stigma), 29.77(disclosure concerns), 35.18(negative self2image)and 57.80(concern with public attitudes toward people with HIV)for Stigma, respectively.Canonical correlation analysis revealed that negative self-image and psychological domain were the main factor for perceived stigma and quality of life, respectively.There was a significant negative correlation between negative self-image and psychological level of quality of life(canonical correlation coefficient 0.4938, P < 0.05).   Conclusion   The less the negative self-image, the higher psychological level of quality of life among people living with HIV/AIDS.
Relationship between(PPD)skin test result and CD4 cell count in HIV/AIDS positive patients
CUI Weiguo, WANG Lin, WANG Zhe,
2005, 21(6): 677-678. doi: 10.11847/zgggws2005-21-06-23
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  Objective   To analyze relation between purified protein derivative PPD skin test result and CD4 cell count in HIV/AIDS positive patients.   Methods   The prospective study was applied.PPD, CD4 and CD8 cell counts were examined between 202 health and 40 HIV/AIDS patients confirmed by western blot.   Results   12.5% of HIV/AIDS patients was PPD positive, meanwhile, 28.2% of health was PPD positive, (P < 0.05).All of the CD4 Cell count below 220/mm3 was PPD negative, the CD4/CD8 below 1 accounted for 90%.   Conclusion   PPD reaction decreases dramatically with the descending of immunity of HIV/AIDS patients.It is caused attention to diagnose tuberculosis in HIV/AIDS patients.
2005, 21(6): 678-678. doi: 10.11847/zgggws2005-21-06-24
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Study on acid phosphatase of blood cells in oncomelania hupensis
TAN Ping, YANG Jianming, XIAO Shaoyu,
2005, 21(6): 679-680. doi: 10.11847/zgggws2005-21-06-25
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  Objective   To study the compatible and resisting mechanism between the early larval stages of Schistosoma japonicum and oncomelania huensis for getting the basic data.   Methods   The hemolymph samples of oncomelania hujpensis were obtained by pressing the foot muscles of the snail.The acid phosphatase(ACP)in the blood cells of oncomelania hupensis was observed at the light microscope level by the burstone stained technique.   Results   The ACP reaction products were seen in the perinuclear region of the spreading blood cells, but they were not seen in the round blood cells.   Conclusion   There is ACP in the spreading blood cells of oncomelania hupensis.
2005, 21(6): 680-680. doi: 10.11847/zgggws2005-21-06-26
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Effect of se-enriched lactobacillus on NK cell activity in cadmium-poisoned rats
CAO Meng, CHEN Long, XU Longbing,
2005, 21(6): 681-682. doi: 10.11847/zgggws2005-21-06-27
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  Objective   To study the effect of Seenriched lactobacillus on spleen NK cell activity in cadmium poisoned rats.   Methods   Seventy-two healthy Sprague-Dawley female rats were chosen and divided into a control group(group C), a cadmium exposure group of low dose(group L-Cd), a cadmium exposure group of medium dose(group M-Cd)and a cadmium exposure group of high dose(group H-Cd).group C, L Cd, M-Cd, H-Cd were orally dosed daily with 0, 2, 5, 10 mg/(kg·bw) cadmium for 9 weeks, respectively.Forty-eight healthy ICR male mice were chosen and divided randomly into a control group (group C), a cadmium exposure group(group Cd), a cadmium plus medium dose Seenriched lactobacillus group(group Cd-MSe)and a cadmium plus high dose Seenriched lactobacills group(group Cd-HSe).Groups Cd, Cd-MSe and Cd-HSe were in jected in the abdominal cavity with 0.5 mg/(kg·bw)cadmium and group C with saline of the same dosage in group Cd for 12 days.   Results   During the entire experimental period, spleen NK cell activity of all groups with chronic cadmium exposure decreased, and displayed a relationship which intensified obviously with dosage increases and time elongation of cadmium exposure.The spleen NK cell activity of group Cd with acute cadmium exposure decreased significantly as compared with group C.The activity of group Cd-MSe was close to group C and superior of significantly higher than that of group Cd.The activity of group Cd-HSe was higher than that of group Cd, but lower than that of group C and Cd-MSe.   Conclusion   A cute and chronic cadmium exposure induced downregulation of NK cell activity.Se enriched lactobacillus could protect effectively the injury in duced by cadmium, and adjusted and ameliorated NK cell activity.
Study on lymphocyte DNA breakup and serum reactive oxygen species level in coke oven workers
WANG Xinchao, XU Yubao, LI Hong,
2005, 21(6): 683-684. doi: 10.11847/zgggws2005-21-06-28
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  Objective   To explore lymphocyte DNA breakup and serum reactive oxygen species(ROS)level in coke oven workers and to screen the earlier biomarkers for occupational pulmonary cancer.   Methods   DNA breakup was detected using the method of single cell gel electrophoresis(SCGE)and ROS level were assayed by the method of Fenton in 39 coke oven workers and 21 normal controls, respectively.   Results   DNA breakup rate was markedly higher in coke oven workers than that of controls(P < 0.01), and DNA damage rate increased with the work age(P < 0.05).The serum ROS level was also much higher in coke oven workers than that of controls(P < 0.05).   Conclusion   The workers exposed to coke oven volatiles could cause the increase of DNA damage rate, and ROS level increase might be involved in this process.
2005, 21(6): 684-684. doi: 10.11847/zgggws2005-21-06-29
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Distribution and expression of active efflux pump acrAB in clinical isolates of Shigella
YANG Haiyan, DUAN Guangcai, XI Yuanlin
2005, 21(6): 685-687. doi: 10.11847/zgggws2005-21-06-30
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  Objective   To study the mechanism of multiple-antibiotic-resistance in clinical isolates of Shigella and to detect the distribution and expression of active efflux pump gene acrAB-tolC.   Methods   Distributions of acrAB-tolC in clinical isolates of Shigella were determined by PCR-SSCP and the levels of acrAB-tolC expression were defermined by Northern-blots.   Results   acrAB-tolC was found in the all strains; the lack of genes was not foud.The mutation of tolC gene was found in four multiple-antibotic-resistance(Mar)of Shigella and the mutation of tolC gene in one susceptible strains; the mutation of acr B gene was not found.The expression level of acrA gene in the Mar strains was significantly higher than that in the susceptible strains; the mutation of acrB gene was not found.The expression level of acrA gene in the Mar strains was significantly higher than that in the susceptible strains(P > 0.05).   Conclusion   High expression of acrA gene leads to multiple-antbiotic-resistance in clinical isolates of Shigella, and Mar operon may contribute to the regulation of acrAB-tolC gene expression.
2005, 21(6): 687-687. doi: 10.11847/zgggws2005-21-06-31
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Expression of apoptosis-related gene in hippocampus of mice after chronic lead exposure
GAO Mingqi, CHEN Hang, ZHOU Bin,
2005, 21(6): 688-689. doi: 10.11847/zgggws2005-21-06-32
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  Objective   To explore the effect of chronic lead exposure on the mRNA expression of bcl-2 and bax in hippocampus of mice.   Methods   RT-PCR techniques were applied to recognize either the mRNA expression of bc-2 and bax in hippocampus of mice exposed to low level of lead.   Results   Bcl-2 mRNA expression in Pb2+-exposed group exhibited a significantly decline and bax mRNA expression in Pb2+-exposed group exhibited a significantly increase compared with control group.   Conclusion   Chronic lead exposure results in significant decline of bcl-2 mRNA expression and significantly increase of bax mRNA expression in hippocampus of mice.
Effect of iodine on immune disorder and thyroid function in EAT rats
TANG Wei, LIU Cao, LIU Cuiping,
2005, 21(6): 690-692. doi: 10.11847/zgggws2005-21-06-33
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  Objective   To investigate the effect of high iodine uptake on the changes of thyroids weight, morphology, thyroid hormaone, thyroid stimulating hormone(TSH)and thyroid autoantibodies..   Methods   Female Wistar rats were divided into four groups: A: normal control, group B: porcine thyroglobulin immunized, group C: iodine water raised, group D: combined with iodine taking and immunization.EAT rat was immunized with PTg 100 ug emulsificated with CFA, then was injected subcutaneously with PTg 100 ug emulsificated with IFA after 2th wk.Rats in group C and D were raised with 0.05% Nal water.Blood samples were taken at 0, 2, 4, 6, 8th wk.   Results   Absolute and relative weight of thyroids in group D were heavier than those in groups A and B, P < 0105.Medium lymphocyte infiltration in thyroid tissue presented in group B, Severe infiltration, proliferation of fibrous tissue and local fulliclar atrophy showed in group D.FT3 and FT4 value in group B increased at 8th wk, but is lower than that in group D, P < 0.01.TgAb and TmAb of group B rose at 2th wk and reached to the peak at 4th wk, then descended a little but still higher than that in group(P < 0.01).The autoantibodies in group C stepped up gradually and reached the peak value at 8th wk.The titer of TgAb and TmAb in group D rose most quickly and lasted for longest time than in other groups.   Conclusion   Iodine may deteriorate Tg2induced autoimmune thyroiditis, enhance the destruction of thyroid gland and elevate the level of autoantibodies.Excessive iodine intake can worsen worsen experimental autoimmune thyroiditis.
Effects of electromagnetic irradiation on MAPK singal transduction pathway of hippocampus neuron in rats
YANG Xuesen, GONG Qianfen, ZHANG Guangbin,
2005, 21(6): 693-695. doi: 10.11847/zgggws2005-21-06-34
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  Objective   To observe the influence of electromagnetic irradiation on mRNA and protein expression of MAPKs families in the hippocampus neuron of rats.   Methods   The rats were acute exposed to 65 mW/cm2 electromagnetic wave 20 min.The mRNA expressions of extracellular signal-regulated 1(ERK1), c-Jun N-terminal kinase1(JNK1), P38 MAPK were tested by RT-PCR.The protein expressions of ERK1, JNK1, P38 MAPK were tested by Western-blot.   Results   Ater electromagnetic irradiation, the mRNA and protein expressions of ERK1 and JNK1 increased significantly in the rat hippocampus.The high expression of ERK1 lasted 12 hours.But JNK1 expressed highly only for 3 hours.AT 24 hours after irradiation, the expression of ERK1 and JNK1 decreased below to the level of control.The mRNA and protein expressions of P38 MAPK didn't change obviously.   Conclusion   The electromagnetic irradiation can influence ERK and JNK signal transduction in gene transcription and protein expression.But ithas no influences on P38 MAPK signal transduction.The ERK and JNK signal transduction may play an important role in the center nerve system induced by electromagnetic irradiation.
2005, 21(6): 695-695. doi: 10.11847/zgggws2005-21-06-35
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Effect of bisphenol A on reproductive system of puberty male rats
GUI Junhong, LU Fuer
2005, 21(6): 696-698. doi: 10.11847/zgggws2005-21-06-36
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  Objective   To explore the possible effects of bisphenol A(BPA)on the reproductive system of puberty male Wistar rats.   Methods   Thirty male rats were randomly divided into 3 groups.The successive intraperitoneal administration of BPA at dose of 1 mg/(kg·bw)and 25 mg/(kg·bw)to rats was conducted in low and high dosage groups for 8 weeks every other day respectively, while rats in control group received the only solvent in equal volume.The body weight, testis and epididymis wet weights, ratios of testis/body weight and epididymis/body weight were determined.The histological observation of the testis and epididymis was carried out and the serum levels of murine sex hormones were detected by radioimmunoassay.   Results   The murine body weights in both low and high dosage groups were significantly reduced as compared with that of control group.Additionally 2 rats in high dose group died in the process of experiment.Furthermore testis and epididymis wet weights, sperm counts of epididymal obviously reduced, and the ratio of testis/weight increased.Optical microscopy showed the interstitial edema of the testis and the interstitial vascular ectasia of the epididymis.   Conclusion   BPA might exert probable adverse effects on murine body weight, development of reproductive system and spermatogenesis of puberty male Wistar rats.
2005, 21(6): 698-698. doi: 10.11847/zgggws2005-21-06-37
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Study on expression of apoptosis and lethal gene during acute rejection in limb allograft
CAO Yang, LIU Wenfeng, LU Xiaofeng,
2005, 21(6): 699-700. doi: 10.11847/zgggws2005-21-06-38
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  Objective   To explore the changes of apoptosis and expression of Fas and Fas-ligand(FasL)during acute rejection of limb allograft.   Methods   Orthotopic limb allograft was performed on inbred SD rat and inbred Wistar rat.Experimental group: allogenic transplant group(SD Wistar), control group: (Wistar Wistar).The grafts were harvested on day 1, 3, 5, 7 after operation.All grafts samples were subjected to histologyical examination and electron microscopic examination.Apoptosis cells of grafts were monitored by terminal UTP nick-end labeling(TUNEL)method, and the Fas/FasL level detected by using immunohistochemistry method.   Results   Histologically, mild, moderate and then severe acute rejection occurred gradually after operation in the experimental group.But none of control group had the histological evidence of acute rejection.The TUNEL showed that the number of apoptosis cells in the experimental group were significantly higher than that in the control.Apoptosis cells were mainly distributed in vascular endo thelial cells and basilar membrane cell of skin.Generally embolism in small vessels occurred due to severe rejection on the day 7 after operation.Fas/FasL expression in allografts were increased in correapondant with the degree of the apoptotic and histologyical damage.   Conclusion   The Fas/FasL mediated apoptosis of vascular endothelial cells play an important role in acute rejection of limb allograft in rats, and might be an important parameter to evaluate the rejection and prognosis in the limb allograft.
Effect of iodine deficiency and hypothyroidism on long-tern potentiation in hippocampus
LIU Lianjie, YIN Hongbo, WANG Ping,
2005, 21(6): 701-702. doi: 10.11847/zgggws2005-21-06-39
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  Objective   To study the effects of iodine deficiency and hypothyroidism on long-term potentiation(LTP)in hippocampus of rat pups, and to discuss the possible mechanism of brain deficits associated with iodine deficiency and hypothyroidism.   Methods   Iodine deficient rats were administered with iodine-deficient diet and hypothyroid rats were administered with methimazole in drinking water.Population spikes(PS)were recorded in CA1 area in response to single pulse stiumulation applied to CA3 area with extracellular microelectrode technique.The mean PS amplitude and peak latency in response to single pulse stimulation were measured before and after high2frequency stimulation(HFS).   Results   The mean peak latencies in iodine deficient and hypothyroid groups before(22.6764±5.4314), (20.0139±3.3028)ms and after(21.4414±5.2231), (20.8592±3.2207)ms HFS were signifiantly higher than that in control group(17.4643±2.6242), (16.7467±2.6283) ms(P < 0.05).The means PS amplitude in iodine deficiency group after HFS(1.8885±0.6418)mV ad hypothyroid group before and after HFS(2.0291±0.3553), (1.9836±0.5698)mV were singnificantly lower than that in the control group (2.4064±0.4645), (3.1610±1.0844)mV(P < 0.05).After the application of the HFS, the mean PS amplitude in the iodine deficiency group decreased significantly(P < 0.05), and 2 out of 9 rats showed LTP, however, 4 showed long-term depression(LTD); the mean PS amplitude in hypothyroid group decreased, and 2 out of rats showed LTP, however, 4 showed LTD; the mean PS amplitude in the control group increased significantly(P < 0.01), and 4 out of 6 rats showed LTP, 1 showed LTD.   Conclusion   Iodine deficiency and hypothyroidism of mother rat ma impair the induction of LTP in vivo of their pups.
Effect of alkalescence ion water on gastric ulcer healing
LIU Jinglan, TAO Yong, LIN Shaobin,
2005, 21(6): 703-704. doi: 10.11847/zgggws2005-21-06-40
Abstract(992) HTML (278) PDF 1004KB(39)
Abstract:
  Objective   To study the effects of ion water on rat gastric ulcer healing.   Methods   To create the rat model of gastric ulcer cauterized by acetum.The rat was treated by drinking alkalescence ion water which pH value was difference and to observe the state of rat gastric ulcer healing.   Results   After drinking alkalescence ion water, the number and index of rat gastric ulcer were significantly decreased(P < 0.05, P < 0.01, respectively), the rate of rat gastric ulcer healing was in creased.   Conclusion   The alkalescence ion water presented significant promotion heal function for rat gastric ulcer cauterized by acetum.
2005, 21(6): 704-704. doi: 10.11847/zgggws2005-21-06-41
Abstract(870) HTML (233) PDF 544KB(26)
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2005, 21(6): 705-706. doi: 10.11847/zgggws2005-21-06-42
Abstract(1008) HTML (200) PDF 195KB(47)
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2005, 21(6): 706-706. doi: 10.11847/zgggws2005-21-06-43
Abstract(888) HTML (259) PDF 412KB(21)
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Effects of perfluorooctane sulfonate on serum thiiodothronine, thyroxine and thyroid-stimulating in rats
ZHANG Yinghua, FAN Yiou, MA Yixin,
2005, 21(6): 707-707. doi: 10.11847/zgggws2005-21-06-44
Abstract(879) HTML (229) PDF 219KB(45)
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  Objective   To explore the effects of perfluorooctane sulfonate(PFOS)on the thyroids of rats.   Methods   Rats were fed forage with 0.5, 1.5, 4.5 mg/kg PFOS.After 65 days, serum thiiodothronine(T3), thyroxine(T4)and thyroid-stimulating(TSH)were analyzed by enzyme exaggeration radioimmunoassays.   Results   Serum T3 and T4 in the PFOS -treated rats were significantly reudced compared with the corresponding animals in control group, but didn't show any dose -effect relationship.There was no substantial idfference of TSH.   Conclusion   PFOS could reduce serum T3 and T4.
2005, 21(6): 708-709. doi: 10.11847/zgggws2005-21-06-45
Abstract(829) HTML (244) PDF 1271KB(29)
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2005, 21(6): 709-709. doi: 10.11847/zgggws2005-21-06-46
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2005, 21(6): 710-711. doi: 10.11847/zgggws2005-21-06-47
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2005, 21(6): 711-711. doi: 10.11847/zgggws2005-21-06-48
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2005, 21(6): 712-713. doi: 10.11847/zgggws2005-21-06-49
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2005, 21(6): 713-713. doi: 10.11847/zgggws2005-21-06-50
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2005, 21(6): 714-715. doi: 10.11847/zgggws2005-21-06-51
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2005, 21(6): 715-715. doi: 10.11847/zgggws2005-21-06-52
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2005, 21(6): 716-716. doi: 10.11847/zgggws2005-21-06-53
Abstract(742) HTML (190) PDF 215KB(30)
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Prevalence and logistic regression analysis on social factors on chronic diseases of middle-elderly people in Henan province
SHI Xuezhong, YANG Yongli, XIE Jing,
2005, 21(6): 717-719. doi: 10.11847/zgggws2005-21-06-54
Abstract(1344) HTML (231) PDF 1612KB(69)
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  Objective   To learn the prevalence of chronic diseases and risk factors of middle elderly people in Henan Province.   Methods   Cluster, stratified and multistage sampling was taken.1 261 residents over 40 years old were assigned to fill in the questionnaires designed by WHO.   Results   The total prevalent rate was 62.7%, 58.1% in male and 68.9% in female.24.6%, 16.0%, and 22.1% of people had one, two, and more than two kinds of chronic diseases respectively.The top three diseases were arthritis and arthropathy, backache or intervertebral disc diseases, gastritis or gastric ulcer.Analysis of multiple factors showed that age, sex, occupation, income, resident surroundings were main social factors affecting prevalent rate of chronic diseases.   Conclusion   The prevalence of chronic diseases of middle elderly people in Henan is higher than that of the people living in another area.Counter measure should be strengthened and more attention should be paid to the elder, the female and the poor man.
2005, 21(6): 719-719. doi: 10.11847/zgggws2005-21-06-55
Abstract(745) HTML (158) PDF 268KB(36)
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Analysis on manpower of community health service in Qindao
ZHANG Dongfeng, YANG Jiulong
2005, 21(6): 720-721. doi: 10.11847/zgggws2005-21-06-56
Abstract(1147) HTML (260) PDF 1017KB(44)
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  Objective   To understand prevalence of community health service manpower in Qingdaocity and to supply basis of rationally disposing health manpowers.   Methods   Random sampling was carried out in a survey for 161 community health service manpower by questionnaire.   Results   The average age of community health service manpower was 43.57, 11.2% manpower were record of graduate; 40.4% were record of junior college, and 28.6% were record of technical secondary school.13.2% staff were high professio nal title; 43.4% were middle professional title and 33.3% were primary professional title.The proportion of manpo wer who was formerly engaged in medicine was 97.5%, 57.1% of them had taken part in the training of general practice.The average training time was 22.74 weeks.   Conclusion   The professional knowledge of health personnel was not well, and health personnel in community health service were low in record of for mal school and the professional title.Those problems should be resolved at once.
2005, 21(6): 721-721. doi: 10.11847/zgggws2005-21-06-57
Abstract(719) HTML (204) PDF 465KB(20)
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Canonical correlation analysis on oculists'quality of life
JING Chunxia, WANG Shengyong, WU Chipeng,
2005, 21(6): 722-723. doi: 10.11847/zgggws2005-21-06-58
Abstract(876) HTML (171) PDF 1001KB(14)
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  Objective   To determine the status of oculists quality of life and influencing factors.   Methods   311 oculists were investigated with WHOQOL-BREF, and a canonical correlation analysis was used to find out the influencing factors.   Results   The scores among the oculists were higher than those of the average in the domain of psycholog y and social relationships.The results showed that there were negative correlations between age, length of service and the domain of Physiology.There were positive correlations between professional title and the domain of environment.   Conclusion   The oculists quality of life was related with age, length of service and professional title.
2005, 21(6): 723-723. doi: 10.11847/zgggws2005-21-06-59
Abstract(649) HTML (179) PDF 372KB(22)
Abstract:
Investigation on AIDS knowledge situation among different populations in Shandong province CARES
TAO Xiaorun, ZHANG Xiaofei, LIU Xuezhen,
2005, 21(6): 724-726. doi: 10.11847/zgggws2005-21-06-60
Abstract(1092) HTML (220) PDF 465KB(114)
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  Objective   To understand the current situation of AIDS knowledge between different populations and to define the contents and ways of health education.   Methods   Cluster sampling was collected from different populations which include countrymen, students in school, paid blood donors, STD clinic clients, sex workers and floating population, and 3833 cases were surveyed by questionnaires.   Results   The rate of the right answers were 51.3%, 47.57%, 45.74%, 53.13%, 57.6%, 39.08% separately in six kinds populations.All kinds of populations obtained AIDS knowledge mainly by watching TV, and so as their enjoyment, and their ratios were 80.51%, 81.3% respectively.   Conclusion   All kinds of populations know little about AIDS.Comprehensive health education is necessary for making them learn more knowledge on HIV/AIDS.The routes people obtaining A IDS knowledge are very little, and is very necessary to make full use of the local medias and all kinds of health resources, and then more choices are provided for people.
2005, 21(6): 726-726. doi: 10.11847/zgggws2005-21-06-61
Abstract(725) HTML (177) PDF 423KB(39)
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Investigation of dietary structure and nutritional status of people aged 50 and over in Beijing
DONG Zong, HUANG Lei, SHA Yimei,
2005, 21(6): 727-728. doi: 10.11847/zgggws2005-21-06-62
Abstract(1186) HTML (258) PDF 884KB(86)
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  Objective   To investigate the dietary structure and nutrition status of people aged 50 and over in Beijing.   Methods   3 day's continuous food weighting and 24 hours food intake recall were used to get the variety of different kind of food and their intake amount.   Results   The major food for the sample population was cereal, and there was difference between urban and ruralarea on the food pattern.Althouth the average levels of some nutrients had close or even over the Reference Nutrient Intake(RNI), the differences among individuals were observed.There was still quite proportion of people whose intake of energy, protein, fat-soluble vitamin, vitamin B group, zinc, potassium and other nutrients were lower than RNI.   Conclusion   The diet pattern of middle aged people was not very reasonable.The intake of vegetable, fruit and pulse products should be increased, and the intake of fat and natrium shoul be controlled.And efforts should be taken to reduce the difference among individuals.
Analysis on health damage in benzene-exposed environment and relevant supervision effect in a shoe-making factory
LIAN Guojian, ZHANG Zhijian
2005, 21(6): 729-730. doi: 10.11847/zgggws2005-21-06-63
Abstract(1725) HTML (358) PDF 1025KB(31)
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  Objective   To understand and to improve the situation of occupational damages by benzene in a shoe making factory.   Methods   To adopt labor hygienic investigational method.   Results   The concentration of benzene in air was about 3.85-52.33 mg/m3 before correction and 1.28-4.01 mg/m3 after correction.Workers clinical symptoms descended after correction.Significant differences were found in symptom morbidity between the control group and contrast group(P < 0.01).Significant changes were found in female workers menses abnor mality before and after supervision(P < 0.05), and significant differences were also found between control group and contrast group(P < 0.01).The count of WBC were found to be descending compared with the contrast group(P < 0.05).The abnormality of Hb had been improved after correction, and became closer to that of the contrast group.   Conclusion   To strengthen the supervision and detection on the occupational hygiene is a significant measure to improve the detection on the occupational damage elements and workers health in developed industrial areas.
2005, 21(6): 730-730. doi: 10.11847/zgggws2005-21-06-64
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2005, 21(6): 731-732. doi: 10.11847/zgggws2005-21-06-65
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2005, 21(6): 732-732. doi: 10.11847/zgggws2005-21-06-66
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2005, 21(6): 733-734. doi: 10.11847/zgggws2005-21-06-67
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2005, 21(6): 734-734. doi: 10.11847/zgggws2005-21-06-68
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2005, 21(6): 735-736. doi: 10.11847/zgggws2005-21-06-69
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2005, 21(6): 736-736. doi: 10.11847/zgggws2005-21-06-70
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2005, 21(6): 737-738. doi: 10.11847/zgggws2005-21-06-71
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2005, 21(6): 738-738. doi: 10.11847/zgggws2005-21-06-72
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2005, 21(6): 739-740. doi: 10.11847/zgggws2005-21-06-73
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2005, 21(6): 740-740. doi: 10.11847/zgggws2005-21-06-74
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2005, 21(6): 741-741. doi: 10.11847/zgggws2005-21-06-75
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2005, 21(6): 742-743. doi: 10.11847/zgggws2005-21-06-76
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2005, 21(6): 743-743. doi: 10.11847/zgggws2005-21-06-77
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2005, 21(6): 744-745. doi: 10.11847/zgggws2005-21-06-78
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2005, 21(6): 745-745. doi: 10.11847/zgggws2005-21-06-79
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2005, 21(6): 746-747. doi: 10.11847/zgggws2005-21-06-80
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2005, 21(6): 747-747. doi: 10.11847/zgggws2005-21-06-81
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2005, 21(6): 748-748. doi: 10.11847/zgggws2005-21-06-82
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2005, 21(6): 749-749. doi: 10.11847/zgggws2005-21-06-83
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2005, 21(6): 750-753. doi: 10.11847/zgggws2005-21-06-84
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2005, 21(6): 753-753. doi: 10.11847/zgggws2005-21-06-85
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2005, 21(6): 754-755. doi: 10.11847/zgggws2005-21-06-86
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Knowledge and belief related to smoking and health and their association with smoking behaviors among secondary school students
WEN Xiaozhong, CHEN Weiqing, LU Ciyong,
2005, 21(6): 756-758. doi: 10.11847/zgggws2005-21-06-87
Abstract(1398) HTML (287) PDF 460KB(176)
Abstract:
  Objective   To explore knowledge and belief related to smoking and health and their association with smoking behaviors among secondary school students.   Methods   Self-administrated questionnaire was applied to the first, second and third year secondary school students in Huangpu district of Guangzhou.   Results   Of the investigated 8 656 students, the mean score of knowledge about diseases caused by smoking(maximum=10)and harmful components in cigarette(maximum=4) was 4.87 and 2.13, respectively.Mean scores of belief related to smoking and health were: 25.88 for effect of smoking (maximum=30), 21.33 for identification with appearance of smoking(maximum=25), 32.07 for identification with sociality effect of smoking(maximum=40)and 32.69 for agreement with measures of preventing and controlling smoking(maximum=40).Ratios for the mean score vs the maximum were 0.86, 0.85, 0.80 and 0.82, respectively.Prevalence of trying smoker, irregular smoker and regular smoker was 16.6%(25.6% for male and 6.3% for female), 12.0%(18.0% for male and 5.1% for female)and 4.7%(7.6% for male and 1.1% for female).In males, the mean score of the knowledge and the belief in regular smokers was the highest, followed by irregular smokers and non-smokers, and the difference was significant in statistics.In females, the mean score of the knowledge was not significant among regular smokers, irregular smokcers and no-smokers, while a signigcant statistics difference was obtained for the mean score of the belief among regular smokers, irregular smokers and no-smokers.   Conclusion   Contrast to the higher score for the belief related to smoking and health, the knowledge related to smoking and health was the lower among the surveyed secondary school students.The knowledge and belief relevant to smoking was associated with different smoking behaviors among the secondary school students.
2005, 21(6): 758-758. doi: 10.11847/zgggws2005-21-06-88
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2005, 21(6): 759-760. doi: 10.11847/zgggws2005-21-06-89
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2005, 21(6): 760-760. doi: 10.11847/zgggws2005-21-06-90
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2005, 21(6): 761-762. doi: 10.11847/zgggws2005-21-06-91
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2005, 21(6): 763-764. doi: 10.11847/zgggws2005-21-06-92
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2005, 21(6): 764-764. doi: 10.11847/zgggws2005-21-06-93
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2005, 21(6): 765-765. doi: 10.11847/zgggws2005-21-06-94
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2005, 21(6): 766-768. doi: 10.11847/zgggws2005-21-06-95
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Abstract:
Cloning of ferritin H chain gene from human Jurkat T lymphocyte induced by arsenic trioxide
WU Shunhua, ZHENG Yujian, ZHANG Yuexin,
2005, 21(6): 973-974. doi: 10.11847/zgggws2005-21-06-21
Abstract(1146) HTML (258) PDF 363KB(33)
Abstract:
  Objective   To understand the differentially expressed genes and to explore the effects on mechanism of gene transcription in human T lymphocytes induced by arsenic trioxide.   Methods   Human J urkat T cell line as cell model was treated by arsenic trioxide(5μmol/L, 24 h)in vitro.Then the differential expressed genes were cloned and the subtractive cDNA library from Jurkat T cell line was constructed by suppression subtractive hybridization.The PCR and sequencing techniques had been applied to testive clones.   Results   The forward subtracted cDNA library from J urkat T cell line induced by arsenic trioxide was constructed.Sequencing analysis showed that there were two clones contained ferritin H chain in the forward subtractive cDNA library.   Conclusion   Arsenic trioxide can induce the transcription of ferritin H chain in T lymphocytes, and the ferritin H chain may play certain role in the mechanism of anti-arsenical cytotoxicity.