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2009 Vol. 25, No. 10

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Perception of gender role, familial affair decision-making and its impact on utilization of prenatal care among rural married women in Xinjiang and Anhui regions
CUI Ying, YANG Li, ZHAO Yan-xia
2009, 25(10): 1153-1154. doi: 10.11847/zgggws2009-25-10-01
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ObjectiveTo understand the perception of gender role and familial affair decision making among rural married women and its impact on the utilization of prenatal care.MethodsIn project counties of Anhui and Xinjiang regions,random cluster sampling was used to select study population and face-to-face interview was carried out.ResultsThere were 65.7% of the women got less than 19 scores when answered questions about perception of gender role,and There were 65.7% of the women got less than 19 scores when answered questions about perception of gender role,and 79.3% of the women got less than 18 scores on questions about the decision-making regarding family affairs.The women was subordinate to men in family affairs.The total coverage rate of antenatal care was 55.7% and 64.0% of the women who received antenatal care got the first antenatal visit during the first trimester.The proportion of women who had at least five antenatal visits was 34 3%.The institutional delivery was 55.1% among all women.Perception of gender role,familial affair decision making and education level were predictors for utilization of antenatal care and in stitutional delivery.ConclusionThe raral women's perception of gender role and the decision making on family affair are weak in Xinjiang and Anhui, as well as the prenatal care utilization.
Influencing factors of menopausal syndrome among women in communities of Tangshan
CHEN Chang-xiang, ZHENG Chun-hua, LI Dan
2009, 25(10): 1155-1156. doi: 10.11847/zgggws2009-25-10-02
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ObjectiveTo explore influencing factors of menopausal sydrom a among women in communities of Tangshan city and to provide basis for effective intervention.MethodsTotally 400 women of 45-55 year-old(from 10 communities of Tangshan, 40 women in each community) were investigated with Kuppenn an scale.ResultsAmong 359 participants,297 people had menopausal symptoms with an incidence rate of 82.73%.The occurrence and degree of menopausal syndrome were significantly correlated with age, working condition, menstruation, spouse status, marital quality, and chronic disease(P<0.05 or P<0.01).Sixty people had depression and the incidence rate was 16.72%.There was significant correlation behveen the occurrence of depression andmenopausal syndrome degree (P<0.01).ConclusionThe influencing factors of menopausal syndrome were complex and should be fully taken into account.
Effectiveness of health education on behaviors of seeking for medical care among married childbearing age women with reproductive tract infections in Gannan region
DU Wei-yun, ZHANG Zhi-lan
2009, 25(10): 1157-1158. doi: 10.11847/zgggws2009-25-10-03
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ObjectiveTo evaluate the effectiveness of health education on behaviors of seeking for medical care among married childbearing age women with reproductive tract infections(RTIs)in Gannan region.MethodsA questionnaire survey was conducted among married childbearing age women selected with stratified cluster sampling technique.Comprehensive health eduction for 14 months was conducted in the intervention group.The uniform questionnaire surey was used to compare the effect of intervention.ResultsAfter the intervention,the ratio of seeking for medical care in symptomatic women was higher than that of before.The ratio increase of the in tervention group was higher than that of the control group.The relative intervention effectiveness was 16.13%.Those of favourable factors influencing seeking for medical care in women with RTIs were accepting intervention,Tibetan nationality,accepting consultation of reproductive health for women,accepting consulation of reproductive health for husband,and having much knowledge about reproductive health.ConclusionReproductive health education is an effective measure to improve behaviors of seeking for medical care among married childbearing age women with RTIs.
Prevalence and type distribution of human papillomavirus infection in women of Datong, China
GUO Jun-cheng, ZHAO Fu-xi, LIU Run-hua
2009, 25(10): 1159-1160. doi: 10.11847/zgggws2009-25-10-04
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ObjectiveTo survey the prevalence and type distribution of human papillomavirus(HPV)infection in women of Datong.MethodsA total of 931 cervical specimens from women attending routine Pap smear cytology screening were subjected to HPV detection and geno typing with PCR-LA assay.ResultsThe overall HPV prevalence was 18.6% in normal specimens,60.2% in specimens with light epithelial internal lesion,69.2% in severe epithelial internal lesion,and 84.6% in specimens with squamous cell carcinoma(SCC),respectively.High risk HPV infections were more prevalent than low risk HPV types(P<0.05).The peak of high risk HPV infection was foundin subjects of 18225 years old.The prevalence of HPV was 15.8%,4.7%,3.8%,and 1.8% for HPV -16,-58,18,and -52 in all participants,respectively.But In SCC,HPV 2.6(61.5%),2.8(11.5%)-58(7.7%),-52(3.8%)and -33(3.8%)were prevalent types.Conclusion The study demonstrated the geographic variability of oncogenic HPV distribution in Datong,China.
AIDS related knowledge and attitude in rural minority pregnant women and parturients
ZHAO Su-ping, HUANG Xiao-rong, GU Yong-xiang
2009, 25(10): 1161-1162. doi: 10.11847/zgggws2009-25-10-05
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ObjectiveTo explore the status of AIDS related knowledge and attitude among ruralmonority pregnant women and parturients in the Qiannan area for developing AIDS prevention strategy.MethodsWith stratified cluster sampling,rural pregnant women and parturients aged 16-34 years in 12 counties were recruited to participate in a face to face interview.ResultsAmong the participants,84.52% heard of AIDS;80.35% knew that AIDS could be pevented; 86.32% knew blood transmision of AIDS;78.26% knew sexual contact transmission;61.36% knew mother to child transmission;33.18% did not know that mother to child transmission could be prevented.The awareness rate of using clear syringes to prevent AIDS infection was 49.85%.The ratios of the pregnant women and parturients without HIV virus antibodies test were 79.2% and 73.85%.The education level was the most important factor influencing the know ledge about AIDS.ConclusionAIDS education should be reinforced to protect rural pregnant women and parturients in minority areas.
Infuence factors of masturbation behavior among female college students majoring in art
CHEN Wei-qi, LI Shi-yue, YAN Hong
2009, 25(10): 1163-1164. doi: 10.11847/zgggws2009-25-10-06
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ObjectiveTo unde rstand the status of masturbation behavior and its influence factors among female college students majoring in art in Wuhan.MethodsAn anonymous questionnaire investigation was conducted among college students majoring in arts selected by randomized cluster sampling in Wuhan.SPSS 11.5 software was employed for statistical analysis.ResultsA total of 749 female college students were srveyed.Among them,18.6% (139/749)had masturbation experience.Masturbation behavior was influenced by growth environment factors and other behavior factor.The students whose mother with low education degree was more likely to have masturbation behavior.The students being not happy in the family during the period of childhood and teenage or browsing over sex-related books,videos or websites were more possibly to have masturbation behavior.ConclusionSome female college students majoring in arts have masturbation experience which is associated with some environment factors and other behavior factor during the period of development.
2009, 25(10): 1164-1165. doi: 10.11847/zgggws2009-25-10-07
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Prevalence and inflnencing factors of depression among rural women in Fengcheng
TIAN Dan, SHI Jing-pu, CHANG Hui
2009, 25(10): 1166-1167. doi: 10.11847/zgggws2009-25-10-08
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ObjectiveTo investigate the prevalence of depression among rural women and to analyze the association beNveen the prevalence of depression and related factors.MethodsWith cluster sampling, 800 women of 18-65 years old were selected The Zung Self-ating Depression Scale (SDS)was applied to assess the extent and severity of depressive symptoms, and an SDS score of 50 was set as the cut-off score Student's t-test, chi-square test and logistic regression were utilized in the study The analysis were performed with SPSS 13.0 software.ResultsThe prevalence rate of depression was 54.3% among the coral women The negative living event scores significantly differed between case group(4.79±13.3055) and control group (2.34±8.4930).The results of logistic regression showed that significant related factors of depression symptom were with high education (OR=0.768, 95% CI=0.638-0.926),having new family members (OR=0.147, 95% CI=0.028-0.769),having heart disease (OR=2.939, 95% CI=1.603-5.390),having conflict with mother in-law (OR=14.938, 95% CI=1.554-143.55),and having adebt (OR=1.861, 95% CI=1.091-3.173).ConclusionThe prevalence of depression among rural women is influenced by physical and social factors.
Perimenopausal syndrome and its influencing factors in wonen of Hebei province
CHEN Chang-xiang, LI Jian-min, YUE Jing-ling
2009, 25(10): 1168-1170. doi: 10.11847/zgggws2009-25-10-09
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ObjectiveTo understand the prevalence and inpactfactors of permenopausal and depressive symptom sinurban and rural women of Hebei for improving permenopausal women's health and quality of life.MethodsWith stratified random sampling, 700 women aged 45-55 years were selected from 7 urban and rural regions of Hebei province Basic information questionnaire, Kuppeun an score sheet and seft-frating depression scale (SDS)were adopted.ResultsAmong the subjects, 95.22% reported permenopausal syndrome; 31.26% had depression.The women living in the town, and without job, with illness partner, divorced, with poor marriage quality, with poor living condition, having bad neighborhood or colleague relationship, with menstrual disorder, without social or family support had higher rate of permenopausal syndrome with significant difference(P<0.05 or P<0.01).The women with permenopausal syndrom a had significant higher rate of depression.ConclusionThe incidence of permenopausal syndroma and depression were higher among women of 45-55 years in Hebei urban and rural areas and the influencing factors were complex.
2009, 25(10): 1170-1171. doi: 10.11847/zgggws2009-25-10-10
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A case control study on association between reproductive factors and risk of breast cancer
PANG Yi, LI Hui, LEI Fang-ming
2009, 25(10): 1172-1174. doi: 10.11847/zgggws2009-25-10-11
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ObjectiveTo explore the association between reproductive factors and breast cancer.MethodsA matched case-control study was conducted from May 2007 to Apr 2008 Overall 119 new diagnosed breast cancer patients in Sichuan Cancer Hospital and 119 health female physical exanainees matched with age were enrolled in the study Reproductive and menstrual factors were investigated with a questionnaire.The data were processed with EPI data 3.0 software, and conditional and un-conditional logistic regression analysis were conducted with EPI Info 2002 software. Results The major risk factor of breast cancer for the cases was the number of miscarriage≥3 (OR=6.10, 95% CI=1.79-20.76, conditional logistic regression).In pre-menopausal sub-group, the OR (95% CI) of the number of miscarriage ≥3 was 4.69(1.10-19.91, unconditional logistic regression).Taking estrogen more than one year was another risk factor for breast cancer in pre-menopausal sub-group (OR=3.69, 95% CI=1.12-12.15, unconditional logistic regression).ConclusionMiscarriage may increase the risk of breast cancer, and taking estrogenic dnzg may increase the risk of breast cancer in pre-menopausal women.
Lead(Pb2+) impairs ability of learning and memory and effects expression of CTGF in hippocampus in mice
LI Ning, YU Zeng-li, ZHENG Yan-tao
2009, 25(10): 1174-1176. doi: 10.11847/zgggws2009-25-10-12
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ObjectiveTo find the effect of lead on learning, memory and the expression of CTGF mRNA and protein in rats for further revealing neurotoxic mechanism of lead.MethodsPregnant mice(10 for ecah group)were exposed to low (0.3 g/L),moderate(1 g/L)and high-level(3 g/L)Pb acetate in deionized drinking water from the first day of gestation to 21 th postnatal day(PND).The lead concentration in blood and hippocampus at the 7th, 14th, 21th day of neonatal mice pups was determined with graphite furnace atomic absorption spectrumetry.The ability of learning and memory and the expression of connective tissue growth factor(CTGF) mRNA and protein in hippocampus of different pup groups at the 21 th day were measured with Water Morris Maze, RT-PCR and inmunohisto chemistry assay.ResultsThe lead concentration in lead exposure group was higher than that of control group (P<0.05).The change of the lead level in blood and hippocampus showed a regular pattern In Water Morris Maze test, the mean incubation period in control group, low,moderate, high lead exposure group were 39.13±6.12, 43.22±9.86, 50.85±12.35, 50.85±12.35 seconds, respectively.The relative expression level of CTGF mRNA in control group, low,moderate, and high lead exposure group were 0.354±0.603,0.945±0.522, 1.223±0.279, 1.612±0.136, and the gray scale of CTGF in hippocampus of pups were 126.10±2.11, 186.31±6.17, 220.94±1.27, 286.21±1.24, respectively.The low lead exposure group showed no statistically significance compared with the control group(P>0.05).However, there were statistical differences moderate and high lead exposure groups(P<0.05, P<0.01).The expression of CTCF mRNA and protein was stronger lead exposure groups than in control group(P<0.05).ConclusionPb acetate exposure in pregnantm ice results in lead accumulation in the body of rat pup, and affects study and memory function of pup mice.The neurotoxicity of Pb may result from the lead-induced high expression of CTCF mRNA and protein.
Related risk factors of breast cancer in female of Wuxi city:a case-control study
QIAN Yun, SHEN Hong-bing, ZHANG Jing-ping
2009, 25(10): 1177-1178. doi: 10.11847/zgggws2009-25-10-13
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ObjectiveTo investigate the risk factors related to breast cancer and the potential interaction between the factors.MethodsA case-control study with 223 newly diagnosed female breast cancer patients and 322 healthy controls was conducted and unconditional Logistic regression was used to evaluate the risk.ResultsEarly age atmenarche(EAM), late age at first and last pregnancy (LAFP and LALP),dysmenorrheal could increase breast cancer risk with adjusted ORs (95% CI) of 1.79(1.26-2.55),1.56(1.10-2.20),1.49(1.04-2.15),1.88(1.22-2.90),respectively.Lactation could decrease breast cancer risk.There were combined effects between EAM and LAFP, EAM and LALP, EAM and not drinking tea,LAFP and not drinking tea, LALP and not drinking tea, family history of breast cancer and not drinking tea (Ptrend<0.01).There were multiple interaction between EAM and not drinking tea LAFP and not drinking tea (Pint=0.029 and 0.000).ConclusionEAM,LAFP and LALP were risk factors of breast cancer, whereas, drinking tea may decrease the risk of breast cancer.Not drinking tea combined with EAM,LAFP,LALP could increase breast cancer risk greatly.
2009, 25(10): 1179-1180. doi: 10.11847/zgggws2009-25-10-14
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Health consciousness and its influential factors among "low-tar" cigarette smokers in six cities of China
XU Ji-ying, LIANG Bo-heng, XIE Fan
2009, 25(10): 1180-1182. doi: 10.11847/zgggws2009-25-10-15
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ObjectiveTo collect information of adult smokers using "low tar" cigarette in Beijing,Shenyang,Shanghai, Changsha,Guangzhou and Yinchuan,and figure out relative influential factors.MethodsWith multiple stage sampling method, 4817 adult smokers were selected and surveyed with face to face interview to collect information of "low-tar" cigarette consumption and relative health consciousness.Data were analyzed with logistic regression to find out influential factors.ResultsThe ratio of smokers reported using "low tar"cigarette was 54.2% in six cities.Compared with regular cigarettes users,"low-tar" cigarette users had significant higher percentages for considerations of "Smoking is not good for health"(82.9% vs 80.6%), "Low-tar cigarettes make it easier to quit smoking(44.1% vs 40.5%)","having plan to quit smoking(27.5% vs 22.3%)", "worrying about smoking will damage health in the future(21.7% vs 15.6%)" and "Smoking is bad or very bad(57.1% vs 50.5%)".There were significant differences in the rate of using low-tar cigarette bewteen six cities.The result of logistic regression analysis showed that different cities,health consc iousness andintention to quit smoking were associated with the behavior of using "low tar"cigarette.ConclusionThere are differences in the rate of using low-tar cigarettes between six cities in China.Health consciousness and the intention to quit smoking are associated with the probability of using "low tar" cigarette.
2009, 25(10): 1183-1184. doi: 10.11847/zgggws2009-25-10-16
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Nutritional iodine status among women in gestational and lactational period in areas with intervention of salt iodization project, Jiangsu province
ZHANG Qing-lan, ZHOU Yong-lin, SHANG Li
2009, 25(10): 1184-1185. doi: 10.11847/zgggws2009-25-10-17
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ObjectiveTo examine the nutritional iodine status amongwomen in gestational and lactationalperiod after the implementation of saltiodization projectin 5 counties, Jiangsu province.MethodsUrine iodine concentration, iodine concentration in salt and salt consumption were measured among the women in gestational and lactational period and without any iodine-rich medications.Estimated iodine intake (EII) was calculated by urine iodine concentration multiplying the average urine voluma The recommended iodine intake (RII) of World Health Organization was referred as evaluation standard.ResultsThe median EII of 1, 243 women was 285.0μg/d, which was 42.5% higher than the WHO's RII (200μg/d).Up to 30% of the women's EII were lower than the WHO RII The average iodine intake from environmental sources was 78μg/d, and the average iodine intake from iodized salt was 208.7μg/d In the marked 11.4%-12% salt samples were tested without any iodine.ConclusionIn the intervention area, the iodine intake of most women in gestational and lactational period reached the WHO recommendatory standard.However, 30% of these women's EII is lower than the standard.There is an increasing trend in non-iodized saltin the market, which may introduce potential risk to women.
2009, 25(10): 1186-1187. doi: 10.11847/zgggws2009-25-10-18
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Knowledge and attitude on gestational diabetes mellitus among pregnant women in Xuzhou
REN Xiang-mei, SHAO Ji-hong, HUANG Shui-ping
2009, 25(10): 1187-1188. doi: 10.11847/zgggws2009-25-10-19
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ObjectiveTo explore the knowledge and attitude on gestational diabetes mellitus(GDM)in pregnant women and to provide data for health education on GDM in pregnant women.MethodsFrom 2006 to 2007,arandom questionnaire and experimental test was conducted among 720 pregnant women in 4 hospitals.ResultsThe rates of knowing all basic knowledge on GDM were lower than 14%.There were statistical differences in knowledge rates between women with different duration of pregnancy,occupa tion,income and education level(P<0.05).Lower knowledged rate were observed among the women with some occupation(peasant, service workers)and those with lower income or education.The percen tages of knowing three basic knowledge on GDM of the pregnants with junior high school were 615, 7.6,and 2.2%.The percentages of admitting the necessity of GDM prevention and of willing to receive health education on GDM among the pregnant women were higher than 90%.ConclusionMost of pregnant women knew alittle of basic GDM knowledge,but the ratio of the need for GDM knowledge was high.
Smoking and passive smoking status in men and women of Yun county, Yunan province
ZHOU Yan, LUO Ting-guang, MAO Wen
2009, 25(10): 1189-1191. doi: 10.11847/zgggws2009-25-10-20
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ObjectiveTo estimate the smoking and passive smoking status in residents of Yun county.MethodsResidents selected with stratified random sampling in Yun county of Yunnan province were investigated.The smoking rate,age beginning smoking,average smoking dose per day,passive smoking rate,years of exposure to environment tobacco smoke (ETS)and daily exposure time of ETS were analyzed with questionnaire survey in men and women.ResultsThe total smoking rate was 34.83% in 1 002 male and female residents.Smoking rate was 71.46% and 2.08% in men and women,respectively.The age of starting smoking was 19.00±5.23 years.The smoking dose per day for average was 17.60±11.13 cigarettes.Total passive smoking rate was 86.37% in non-smokers and passive smoking rate inmale and female was 79.25% and 88.22%,respectively.51.60% of the passive smokers exposed to ETS since they were born.The daily exposure time was 3.98 ±3.43 hours in man and 4.11±3.60 hours in women.ConclusionThe women have higher passive smoking rate,longer daily exposure time and ear lier exposure to ETS.Women are the main victims of passive smoking.
Reproductive health status of 3052 married women in rural of Chaohu city
SHEN Qiong, ZHANG Xiu-jun, YU Yu-ling
2009, 25(10): 1191-1192. doi: 10.11847/zgggws2009-25-10-21
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ObjectiveTo study the reproductive tract infections(RTIs) of marriedwoman in rural communities of Chaohu city and its related influencing factors in order to promote reproductive health and adopt interfering measures.MethodsTotally 3052 married women selected with stratified cluster sampling were investigated with clinical examination and questionnaire survey on RTIs related health care knowledge, attitude and behavior Results Among the 3052 married women there were 972(31.8%)had at least one RTI,289 (9.5%)had two RTIs The rate of cervical erosion, bacterial vaginitis, candida, trchomonasis and vulvitis was 18.4%,3.93%,3.87%,2.2%,and 0.8%,respectively.The results of Logistic regression showed that the influence factors of RTIs were education, husbands occupation, abortion, the first bearing age, the number of the children and the interval of bathing in winter.ConclusionRTIs are prevalent in the rumlwomen and affected by multi-factors.We should carry out health education and gynecological census to decrease RTIs.
Warning effects of health labeling on cigarette packet on smokers in six cities
LI Xin-jian, JIANG Yuan, LI Qiang
2009, 25(10): 1193-1195. doi: 10.11847/zgggws2009-25-10-22
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ObjectiveTo examine the effects of health warning on cigarette package on smokers in Beijing,Shenyang,Shanghai, Changsha,Guangzhou,and Yinchuan.MethodsMultistage sampling was used to select 4815 smokers in six cities.Face to face interview was conducted to colledtrelated information.Multivariate logistic regression models were used to explore factors associated with warning effects.ResultsAmong the participants,94.9% was male.Average score of warning effects was 1.38(effective score≥2).Only 2.15% of respondents often stop smoking because of warning labels, and 13.31% avoided warnings during the past one month.The proportion of considering the harm of smoking and planning to quit smoking because of noticing the warning label were only 8.26% and 5.29%,respectively.Multivariate logistic regression analysis showed that different cities and smoking years were associated with warning effects of health labels on cigarette package.ConclusionCurrent health warning on cigarette package had no designed warning effects for smokers.It is necessary to renew the form of the warnings.
Customer satisfaction to service of vaccination clinics in Ji'ning
ZHANG Jing, WANG Wen-jun, LI Jing
2009, 25(10): 1195-1196. doi: 10.11847/zgggws2009-25-10-23
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ObjectiveTo understand customer satisfaction to the service of vaccination clinics for improving immune planning strategy.MethodsRandom cluster sampling was used and 667 parents from 5 communities of Ji'ning were surveyed with a self-designed questionnaire.ResultsAmong the participants, 60.8% considered the vaccination service as good, 33.3% as not bad and 5.9% of the participants were unsatisfied to the service.The results of polytomous logistic regression on ordinal response displayed that variables which affecteded customer satisfaction were sanitation status of vaccination clinics (OR=2.74),the respect of clinic staffs to customer(OR=2.67),the skill of doctors and nurses (OR=2.64),medical knowledge of doctors and nurses (OR=2.15),the introduction of vaccination relevant knowledge (OR=1.76),the method of notice (OR=1.71),whether to conduct examination before inoculation (OR=1.55) and whether to rem ind key points after inoculation (OR=1.52).ConclusionThe customer satisfaction to service of vaccination clinicis related to technical skills, service mode and quality of doctors and nurses and closely related to environmental factors and psvchological state of the customers.
2009, 25(10): 1197-1198. doi: 10.11847/zgggws2009-25-10-24
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Health-related quality of life in women of rural area
WANG Jian, MENG Qing-yue, QU Jiang-bin
2009, 25(10): 1198-1199. doi: 10.11847/zgggws2009-25-10-25
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ObjectiveTo understand the health-related quality of life(HRQOL)and its influencing factors in rural women.MethodsThe sumple was randomly chosen with stratified cluster sumpling and 1653 coral women from Anyue of Sichuan, Linquan of Anhui, Caoxian and Chiping of Shandong were investigated HRQOL was measured by EQ-5D.SPSS 13.0 software was used in analysis.ResultsThe average health index of women was 70.3.Among four counties, Caoxian was the highest(75.8),which was 13 points higher than that of Anyue Women's health index declined with aging and the young group was 8 and 16 points higher than that of middle-aged group and elder group.With the increase of education level, the health index increased The illiteracy group was 66.6, while the middle school and higher level education was 77.6.The difference was mainly in the feeling of pain/discomfort, anxiety/depression of the five health dimensions There were 34.3% and 27.5% of the women with pain/discomfort or anxiety/depression.ConclusionEnhancing education degree, improving living environmentand effective psychological intervention can improve the HRQOL of rural women.
Effects of diesel exhaust particles on airway hyperresponsiveness and immunologic index in rats
SU Li-ming, CHI Yong-xue, YUAN Xi-zhe
2009, 25(10): 1200-1201. doi: 10.11847/zgggws2009-25-10-26
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ObjectiveTo investigate the effects of disesel exhaust particles in air pollutants on airway hyperre sponsiveness and immunologic index in rats with asthma.MethodsSixty Wistar rats were rendomly dividedinto 6 groups.Grup A was control group.Group B was animal model group which was ovalbumin(OVA)sensitized challenged.Group C,D,E,F were challenged with 3% OVA and then exposed to diesel exhaust particles(DEP)for one to four weeks.The levels of IgE,interleukin 5(IL-5)and interferon(IFN-γ)of the lung tissue were detected by ELISA.ResultsThe airway hyperre sponsiveness of group B increased more than group A.Group B was significantly different from group E and F(F=156.186,P<0.01).Increase of airwag resistance was related with the time of DEP exposure(r=0.948,P<0.01).The concentration of IgE was significantly different between group A and B(F=2.490,P<0.01).The expression of IL-5 in lung tissue gradually increasedin group C.The expression of IL-5 in group B was significantly different from that of group C and E(F=4.263,P<0.01).The expression of IFN was not significantly different among the groups(F=1.193, P>0.05).ConclusionExposure to diesel exhaust particles might exacerbate airway hyperrespon siveness andinduce airway in flammation caused by IL-5 increase in the rats of asthma model.
CYP1A1 and GSTM1 polymorphisms in Miao population of Guizhou province
LEI Ting-wen, RAO Min
2009, 25(10): 1202-1203. doi: 10.11847/zgggws2009-25-10-27
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ObjectiveTo explorecy to chrome P450 1A1(CYP1A1)and glutathione Stransferase M1(GSTM1)genetic polym orphisms in Miao population of Guizhou.MethodsCYP1A1 and GSTM1 gene polymorphisms among 97 Miao people were studied with polym rase chain reaction(PCR)and polymerase chain reaction restriction fragment length polymorph sm(PCR-RFLP)technique.ResultsThe allele frequency of CYP1A1m1 was 0.195;the geno types of CYP1A1m1 T T,TC and CC were 71.13%,18.56% and 10.31%,respectively.The frequency of GSTM1 null geno type was 69%.ConclusionThe allele frequency of CYP1A1m1 in Miao population was higher than Caucasian,but lower than Chinese Han opoulation.The frequency of GSTM1 null geno type was higher than German,Polish,Japanese and Korean.
Expression and identification of soluble wbbL protein of M. tuberculosis in Escherichia coli
WU Qiang, XIN Yi, MA Yu-fang
2009, 25(10): 1204-1206. doi: 10.11847/zgggws2009-25-10-28
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ObjectiveTo express wbbLprotein of Mycobacterium tuberculosis in E.coliBL21 (DE3) underoptinistic conditions, and to obtain and identify the soluble protein expressed.MethodsThe pET16b-Tb wbbL was expressed in E.coliBL21 (DE3) under different induction conditions.The cexpression system for expressing chaperons of pKJE7 plasmid and soluble wbbL protein was through condition inprovement Both supernatant and pellet fractions of wbbL protein expressed were analyzed with SDS-PAGE and western blotting methods after ultrasonic processing.ResultsThe results demonstrated that the wbbL protein was obtained in soluble fraction from co-expression system induced by the optinization concentration of 0.5 g/L-arabinose under 30℃ for 5hr and 0.5mmol/L IPTG under 30℃ for 2hr.The recombinant protein constituted 55.6% of the total protein of the cells.The wbbL gene was expressed into wbbL protein correctly Conclusion The soluble wbbL protein from E.coliBL21 (DE3) carrying pET16b-Tb wbbL and pKJE7 plasmid was acquired.The purified wbbL protein could be utilized to study its kinetics and to develope an enzyme assay for screening drugs.
Silicosis fibrosis model through macrophage injury in rats
LI Yu-hu, MIAO Zhu-lei, CHENG Guo-qiang
2009, 25(10): 1206-1207. doi: 10.11847/zgggws2009-25-10-29
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ObjectiveTo study the possibiliy and degree of macrophage injury and collapse induced by SiO2.Methods Sterile SiO2 solution(1ml)was injected in to abdominal cavity of Wistar rats one time.The peritoneal fluids of the rats was taken at 1st-3rd day after the exposure to observe the phayocytose and injuries of macrophages.ResultsTypical cytopathic,damage and finally breakdown resulted from the injection of SiO2 particles were observed 4 hours after the injection.The damage rate of macrophage was up to 10%.ConclusionThe animal model of silicos is was established and could be adopted in pathogenic study of silicosis.
Case-control study on HLA-DRB1*0701, environmental risk factor and non-or hyporesponse to hepatitis B vaccine
CHEN Si-dong, HUANG Xiao-hui, YANG Yi
2009, 25(10): 1208-1210. doi: 10.11847/zgggws2009-25-10-30
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ObjectiveTo confiun the association between HLA-DRB1*0701 and environmenmtal factor such as smoking, drinking with the non-or hyporesponse to hepatitis B vaccine.MethodsA case-control study included 128 non-or hyporesponders and 128 responders from 1 342 healthy adults who were subjected to a standard course of inmunization with recom binant hepatitits B virus vaccine All subjects were investigated with a unifoun questionnaire HLA-DRB1*0701 genes was detected with ploymemse chain reaction-sequence specific prines(PCR-SP).The data were analysed with SPSS 13.0 software.ResultsUnivariate analysis indicated that age (OR=2.52, 95% CI 1.51-4.21),BMI (OR=2.38,95% CI 1.13-5.02),smoking (OR=2.15, 95% CI 1.08-4.28) and drinking tea (OR=0.32, 95% CI 0.10-0.99) were associated with non-or hyporesponse.The DRB1*0701 allele was found at a ratio of 16.4% in the non-or hyporesponders which was significant when compared with the responders(3.9%,χ2=10.959, P=0.002, OR=4.83).The results of multivariate logistic regression showed that only 3 factors entered the model, including age (OR=2.12,95% CI 1.24-9.69),BMI(OR=2.21, 95% CI 1.04-4.72),DRB1*0701 (OR=4.08, 95% CI 1.45-11.46).ConclusionMany factors were associated with non-or hyporesponse to hepatitis B raccine.
2009, 25(10): 1210-1211. doi: 10.11847/zgggws2009-25-10-31
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Overexpression of MDR1 is associated with acquired tolerance to arsenic in L-02 cells
SHEN Xu-bo, ZHOU Yuan-zhong, JIANG Hui
2009, 25(10): 1212-1214. doi: 10.11847/zgggws2009-25-10-32
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ObjectiveTo study the relativity of multidrug resistance gene 1(MDR1)gene expression and acquired to lerance to arsenic in human hepatocyte cell line(L-02).MethodsThe levels of MDR1 mRNA were determined by real time quantitative PCR,and the expression of P-gly coprote in(P-gp)was examined by mimunohisto chemical SABC in both arsenite exposed cells and the control.Then the cells were incubated with fresh medium containing 0,2 5,5 0,10.0 mol/L NaAsO2 in the pre sence or absence of 10 mol/L PSC833(an inhibitor for Pgp).Twenty four hours later,cellular total arsenic was determined using graphite furnace atomic absorption spectrometry,and 3-[4,5-dimethy lthiazol-2-yl]-2,5-diphenyl tetrzolium(MTT)was used to detect cell survival rate.ResultsThe expression of MDR 1 mRNA and P-gp in arsenite exposed cells(8.890±0.968,68.76±3.81%)was higher than in L-02 cells(1.014±0.189,24.44±4.03%), (P<0.001);the survival rate of arsenite exposed L-02 cells(109.630±0.511%,95.469±0.054%,78.890±0.024%) was apparently higher than that of L-02 cells(104.841±0.015%,91.534±0.026%,64.811±0.079%),(P<0.001); cellular arsenic content in arsenite exposed cells(0.68±3.65×10-03,1.36±4.72×10-03,3.27±9.62×10-03 μg/L)was markedly decreased than in control(1.17±5.48×10-04,3.66±1.95×10-03,7.53±6.20×10-03 μg/L).With 10 mol/L PSC 833,cellular arsenic content was markedly increased and survival rate of the cells was apparently decreased (P<0.001).ConclusionAcquired to lerance to arsenic is associated with increased expression of MDR1 with presentation of mRNA and prote in in human hepatocyte cell line.
Prediction and analysis for structure and function of adenylate kinase gene from Taenia solium by bioinformatics
DAI Jia-lin, HUANG Jiang, LIAO Xin-jiang
2009, 25(10): 1214-1215. doi: 10.11847/zgggws2009-25-10-33
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ObjectiveTo analyze and predict the structure and characteristics of adenylate kinase gene from Taenia solium by bioinformatics for its biological function and application research.MethodsWith analyzing tools of bioinformatics in webs site and bioinformatics software package,adenylate kinase was recognized from the Taenia solium full-length cDNA plasmid libratory andits gene structure and coding region sequence was analyzed andits physical-chemical characteristics,topo logical structure and 3 Dstructure were predicted.ResultsThe full gene is 864 bp in length and its coding region is 110-758,coding 215 amino acids.It is a complete full-length gene comparing with the homologues in Gen Bank.The prote in has no transmem brane region.The molecular weight of adeny late kinase gene is predicted to be 23771.4Da.The proe in contains no transmem brane region and subcellular location sequecce.The predicted amino acid sequence shows 66% identity and 84% smiilarity with that of ADK form Schisto somaja ponicum.ConclusionThe cDNA sequence encoded adenylate kinase gene was screened from cDNA library of adult Taenia solium by bioinformatical method.The gene and prote in structure and characteristics of Taenia soliumadeny late kinase were obtained.
Serum pepsinogen level in health persons and patients with chronic gastric disease or stomach cancer
ZHU Lan, HUANG Biao, ZHANG Jue
2009, 25(10): 1216-1217. doi: 10.11847/zgggws2009-25-10-34
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ObjectiveTo investigate serum pepsionogen(PG)level in health persons and patients with chronic gastric disease or stomach cancer.MethodsSerum PG level was detected by TRFIA.The correlation between the levels of serum PGⅠ,PGⅡ and PGⅠ/PGⅡ and the mucosal changes in the patients with chronic gastropathy was analyzed.ResultsThe normal reference range of PGⅠ,PGⅡ and PGⅠ/PGⅡ was 60-252μg/L,≤27.9μl/L and PGⅠ/PGⅡ≥6, respectively.The level of serum PGⅠ,PGⅠ/PGⅡ in male were significantly higher than those of in female(P<0.05).PG Ⅱ had no significant difference in gender.There was a significant decrease in PGⅠ/PGⅡ ratio along with the increase of age.The level of serum PGⅠ gradually reduced with the progress of chronic superficial gastritis(CSG),chronic atrophic gastritis(CAG),and gastric cancer(GCa).The SCa group showed a high detection rate of PGⅠ≥240μg/L and ≤60μ g/L.The possitive rate of PGⅠ≥240μg/L was significantly higher in gastriculcer(GU)group.The ratio of serum PGⅠ/PGⅡ in CAG group and SCa group were signcficantly lower than other groups.Serum PGⅠ≥240 or ≤60μg/L and PGⅠ/PGⅡ≤6 were good cut off value for the screen of GCa.ConclusionThe serum PG level showed a skew distribution and significantly rose with sex and gastric mucosal changes.
Efficacy of anti-HIV treatment and drug-resistance mutations in Henan province, China
CUI Wei-guo, HU Qing-hai, LI Hong
2009, 25(10): 1218-1219. doi: 10.11847/zgggws2009-25-10-35
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ObjectiveTo study the virological and inmunological effcacy of HAART among Chinese HIV/ASS patients and its influence of drug resistance mutations.MethodsViral load, CD4+ Tlymphocytes count and drug resistance genotype were detected in patients after two reginens treatment.ResultsUndetectable viral load level and CD4+ Tbmphocytes maintenance were achieved among a part of HIV/AIDS patients, but drug resistance mutations happened during the two reginens.High drug resistance mutation rates of D4T/ddI/NVP, AZT/ddI/NVP were 19.7%,16.2% in 2005, and 19.4%,19.5% in 2006.ConclusionExpected efficacy was achieved in Chinese HIV/ASS patients But the high prevalence of drug resistance mutations against reverse transcriptase inhibitors, crossing drug resistance, and multi-drug resistance suggest that more attention should be paid to the drug resistance mutation detection, treatment standardization.
Relationship between the contents of IL-8, TNF-α in seminal plasma and sperm dynamic indexes in infertile patients
YE Song-dao, WEN Huai-kai, ZHU Hai-bo
2009, 25(10): 1220-1221. doi: 10.11847/zgggws2009-25-10-36
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ObjectiveTo observe the relationship between the contents of interleuk in-8(IL-8),tumor necrosis factor alpha(TNF)in seminal plasma and sperm dynamic indexes in infertile males.MethodsBy radio mimuno assay(RIA), we determined the contents of IL-8 and TNF-α in the seminal plasma of 75 infertile(sperm count≥20×106/ml)and 22 normalmales.Accoding to sperm vitality and motility,the infertiles were subdividedintoa normal and abnormal vitality groups and normal and decre sed motility groups.ResultsThe content of IL-8,TNF-α in the seminal plasma of the infertile group was obviously higher.But many dynamic indexes,including sperm vitality,sperm motility and sperm speed, were significantly lower than that of the normal group(P<0.05 or P<0.01).In the infertility group,there existed significant differences in the contents of TNF-α be tween the normal and decreased sperm vitality,motility groups (P<0.05).There existed significant differences in the contents of IL-8 between the normal and decreased sperm motility groups.The contents of TNF-α were negatively correlated with sperm vitality,motility and speed(P<0.05).The content of IL-8 had a significant negative corelation with sperm motility(r=-0.39,P<0.05),but had no obvious relation to other dynamic indexes.ConclusionThe contents of IL-8,TNF-α inseminal plasma are closely related to sperm dynamic indexes.Determination of IL-8,TNF-α contents can reflects sperm functions,and afford valuable evidence in exploring the mechanism of infertility and in clinical medication.
Cloning, sequencing of myosin gene from periodic Brugia malayi and prediction of B cell epitopes in its amino acid sequence
XIE Dong-fang, FANG Zheng, TONG Hai-yan
2009, 25(10): 1222-1224. doi: 10.11847/zgggws2009-25-10-37
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ObjectiveTo clone and sequence the myosin gene of periodic Brugia malayi(BmM yosin)and to predict the Bcell epitopes of encoded peptide sequence of the gene.MethodsTotal RNA was extracted from periodic Brugia malayi.Acouple of specific primers were designed on the basis of known sequences of myosin gene from Brugia malayi.The desired gene was amplified by PCR technique from cDNA.The PCR products were purified and cloned in to plasmid pGEM-T by T-A cloning method,and transformed in to Escherichia coli(E.coli)strain DH 5α.The recom binant plasmids were screened and identified by digestion with restriction enzyme and PCR amplification.The positive recombinant plasmid of pGEM-T BmM yosin was confirmed by sequencing and homology comparison.Five parameters and methods were used to predicate B-cell epitopes in aminoacide sequence of BmM yosin.ResultsA specific band around 1 292 bp was amplified by RTPCR.The same product was obtained by double restriction enzyme digestion of recombinant plasmid and PCR.The result of DNA sequencing shows that BmM yosin shares 98.45% nucleotide sequence with that of known sequences in the gene bank.B cell epitopes of BmM yosin are probably at or adjacent to 287-300,339-350 and 416-422 in its am ino ac id sequence.ConclusionpGEM-T BmM yosin of periodic Brugia malayi was successfully constructed,which provides the basis for further study of BmM yosin expression and functions.
Development and evaluation of quality of life instrument for patients with chronic pulmonary heart disease
WANG Guo-hui, LI Xiao-mei, WAN Chong-hua
2009, 25(10): 1224-1226. doi: 10.11847/zgggws2009-25-10-38
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ObjectiveTo develop and evaluate the quality of life instmment for chronic pulmonary heart disease (QL ICD-PHD).MethodsThe programmed decision-malong method was used to develop the scale.The reliability, validity and responsiveness of the instrument were evaluated by analyzing the data from 120 patients with chronic pulmonary heart disease Statistical descriptions, Pearson's correlation coefficient, exploratory factor analysis, structural equation model, paired-samplest test were used in the analysis.ResultsThe QLICD-PHD was developed with a 30 items general module and a 21 items specific module Most of correlation coefficients between each item and its domain were above 0.5, and greater than the correlation coefficients between the item and other domains The exploratory factor analysis and structural equation model showed good validity of the instrument The Cronbach's Alpha in each domain and total score were greater than 0.75.The test-retest reliability coefficients were greater than 0.927, and split-half reliability coefficients were greater than 0.712 The instrument had good reliability.Pairedt test and standardized response means between before and after treatment showed good responsiveness of scale.Most of patients could finish the questionnaire in 15 minutes.ConclusionThe QLICD-CPHD is valid, reliable, sensitive and reasonable operation in clinical settings, and it can be used to assess quality of life for patients with chronic pulmonary heart disease.
Development of fluorescence quantitative PCR for detection of genogroup Ⅰ Sapporo-like viruses in shellfish
ZENG Ai-hua, MEI Han-fang, YANG Xiao-rong
2009, 25(10): 1227-1228. doi: 10.11847/zgggws2009-25-10-39
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ObjectiveTo develop a fluorescence quantitative polymerase chain reaction(FQ-PCR)for the detection of geno group I(GI)Sapporo-like viruses(SLVs)in shellfish.MethodsSLVs were concentrated by PEG 6000.The degenerate primers and probe were designed following large scale SLVs genome consensus analysis and subsequently a FQ-PCR assay for detection of GISLVs was established.ResultsT the assay developed possessed high accuracy and repetition for SLVs detection.The sensitivity of the assay was as low as 102 copies per reaction.The assay was linear within 5-log dynamic range between 102 copies and 106 copies.The correlation coefficient of the standard curve was 0.9988.ConclusionThe detection method of geno group I Sapporo-like viruses was established with FQ-PCR and can be used for rapid detection of SLVs pollution in shellfish and emergency of SLVs.
Levels of antibodies of measles, mumps and rubella in children of Shaanxi
LI Ping, SI Yuan, GUAN Rong-hui
2009, 25(10): 1229-1230. doi: 10.11847/zgggws2009-25-10-40
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ObjectiveTo unde rstand an tibody levels of measles,rubella and mumps in healthy children of Shaanxi.MethodsTotally 225 blood samples from healthy children of 1-14 year old were assayed for antibodies of measles, rubella and mumps.ResultsThe positive rate of meas les was above 94%.The average geometric means of antibody titer for rubella and mumps were higher(27.74,28.94)among urban children of 1-2 years old with a decreasing trend as the increment of the children's age.The positive rates of rubella and mumps were lower among rural children of 1-2 years old and increased with the age.ConclusionThe immunity agains trubella and mumps is conside rably low in healthy children and the one year old children should be vaccinated in Shaanxi province.
Effects of MPP~+ on proliferation and ERK activation of PC12 cells
ZHENG Gang, LUO Wen-Jing, ZHANG Xue-Ping
2009, 25(10): 1231-1233. doi: 10.11847/zgggws2009-25-10-41
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ObjectiveTo study the toxicity of 1-methyl-4-phenylpyridinium ion (MPP+)on rat pheochrom ocytom a PC12 cells in vitra.MethodsPC12 cell cultures were exposed to 100, 300, 500 μmol/L MPP+.The inhibition of the cell proliferation was deteanined by MTT assay Western blot was performed to determine the level of phosphorylated extracellular signal-regulated protein kinase(ERK).ResultsMPP+ exposure inhibited the proliferation of PC12 cells in a dose-and time-dependentm anner.The inhibitory rates were 17.86%-58.06%.Western-blot showed that MPP+ decreased the phosphorylation of ERK1/2(P<0.01).PD98059, a selective inhibitor of MEK, further inhibited the proliferation of PC12 cells.ConclusionMPP+ can significantly inhibit the proliferation of PC12 cells The reduction of ERK1/2 phosphorylation could be an inportant molecular mechanism of MPP+-induced dopam inergic neurotoxicity.
Effectiveness of sanitary toilet construction in rural areas with schistosomiasis epidemic
WEI Hai-chun, FU Yan-fen
2009, 25(10): 1233-1234. doi: 10.11847/zgggws2009-25-10-42
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ObjectiveTo comm unicate the experiences of schistosom iasis control, and assess the effectiveness of rebuilding toilet program in schistosom iasis regions.MethodsSeven provinces with current schistosom iasis epidem is including Hunan, Hubei, Anhui, Jiangxi, Yunnan, Sichuan and Jiangsu provincewere the settings for the study.Two counties in each province and 3 villages in each county were randomly sampled.Among 3 villages selected, two had sanitary toilets, and the other did not Twenty households were randomly sampled from each village for field study.ResultsThe schistosom iasis infectious rate and diarrhea incidence rate in the areas with the construction of sanitary toilets was 47.32% and 42.31% lower than that of the contrast areas.In the areas with construction of sanitary toilets, the schistosom iasis prevalence among the population using sanitary toilets was 63.5% lower than that of without sanitary toilet use.ConclusionConstruction and popularization of sanitary toilet in the rural areas with schistosom iasis epidemis is proved to be an effective measure for schistosom iasis incidence and diarrhea incidence control and achieves significant health effectiveness.
Prevalence and related risk factors of nonalcoholic fatty liver among administrative officials
PENG Xian-e, HUANG Zhi-min, LIN Jian-yin
2009, 25(10): 1235-1237. doi: 10.11847/zgggws2009-25-10-43
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ObjectiveTo investigate the prevalence of nonalcoholic fatty liver and its risk factors among a specific population in Fuzhou city.MethodsA total of 3 884 administrative officials who denied regular alcohol drinking participated in the survey Questionnaire survey, physical examination, serum lipid-profile, 75 gramoral glucose tolerance test and ultrasonogmphic examination of liverwere conducted.ResultsThe age-sex-adjusted prevalence of nonalcoholic fatty liver in the participants was 27.45%.The age-adjusted prevalence of nonalcoholic fatty liver in themale was 35.70% and significantly higher than that of in fem ale(17.47%,P<0.05).The prevalence of obesity, hypertension, dyslipidemia, high blood uric acid and low high-density lipoprotein cholesterol(HDL-C)in the nonalcoholic fatty liver group were significantly higher than those of in the group without fatty liver(all P<0.05).Multiple variant regression analysis showed that there were positive correlations between prevalence of nonalcoholic fatty liver and factors such as sex, age, obesity, hyperlip idemia, hypemricaemia,low HDL-C and inpaired glucose tolerance Conclusion There is a high prevalence of nonalcoholic fatty liver among administrative officials in Fuzhou.Metabolic disorders such as obesity, hyperlip idemia, and hyperuricaemis are significantly related to nonalcoholic fatty liver.
2009, 25(10): 1237-1237. doi: 10.11847/zgggws2009-25-10-44
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Prevalence and risk factors of coronary heart disease among senior intellectuals
RUAN Liang, XIAO Yong-kang, HU Chuan-lai
2009, 25(10): 1238-1239. doi: 10.11847/zgggws2009-25-10-45
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ObjectiveTo analyze prevalence and risk factors of coronary heart disease among senior intellectuals in Hefei and to provide reference for prevention and control of the disease Metbods A total of 4726 subjects above 35 years old were selected with cluster sampling The prevalence of coronary heart disease in different gender and age groups, and factors related to the disease were analyzed with non-conditional logistic regression Results In the survey, 256 coronary heart disease cases were identified with a prevalence of 5.6%.The prevalence was 5.1% in the male, and 6.9% in the female(χ2=6.126, P<0.05).The prevalence of coronary heart disease increasedwith age(male: χ2=149.056, P<0.001; female: χ2=111.099, P<0.001).Multi-variate logistic regression analysis showed that factors associated with coronary heart disease were age (OR=3.627, 95% CI=2.905-4.529, P=0.000),gender(OR=0.692, 95% CI=0.501-0.955, P=0.025),family history of coronary heart disease (OR=2.347, 95% CI=1.656-3.327, P=0.000),hypertension (OR=1.537, 95% CI=1.144-2.064, P=0.004),waist-hip ratio (OR=1.463, 95% CI=1.091-1.961, P=0.011) and salt intake(OR=1.236, 95% CI=1.009-1.516, P=0.041).ConclusionThe prevalence of coronary heart disease in senior intellectuals in Hefei was higher and comprehensive intervention measures should be taken against the risk factors of the disease.
Study on DNA damage and cell apoptosis in NRK cells after treatment with cadmium chloride
XU Xin-yun, DING Li-ping, LI Xue-yu
2009, 25(10): 1240-1241. doi: 10.11847/zgggws2009-25-10-46
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ObjectiveTo study DNA damage and cell apoptosis in NRK cells after treatment with cadmium chloride.MethodsNRK cells were treated with various doses of calmium chloride for 12 hr.Hoechst/PI double fluorescent staining assay was applied to detect cell apoptosis, and single cell gel electrophoresis (SCGE) was used to test DNA damage after NRK cells were treated with calmium.ResultsThe rotes of cell apoptosis in groups treated with 2.5, 5, 10, and 20μmol/L cadmium chloride were 5.9%,12.4%,24.6%,and 46.2%,respectively, which were higher than that in control group.On the other hand, the tail DNA content and DNA tail length significantly increased in the groups treated with cadmium chloride when compared with the control.Furthermore, the tail DNA content and DNA tail length showed dose-dependent response after calmium treatment.ConclusionCadmium could induce cell apoptosis and DNA damage in NRK cells DNA damage might be the reason of cell apoptosis.
Effects of manganese on lipid peroxidation in lung and liver of mice
JING Jun-jie, XIE Ji-min, CHEN Min
2009, 25(10): 1242-1244. doi: 10.11847/zgggws2009-25-10-47
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ObjectiveTo explore the effects ofmanganese(Mn) on lip idperoxidation and contents of iron and zinc in the lung and liver of mice and the mechanism of manganese toxicity.MethodsAfter single exposure to MnCl2(100mg/kg body weight) via i p.in 30 mice, the activity of aspartate am inotransferase (AST),alanine am inotransferase (ALT) and lacatate dehydrogenase(LDH)were deteunined at 1st, 2rd, 4th and 7th day The lipid peroxidation(LPO)and concentrations of Mn, calcium (Ca),zinc (Zn) and iron (Fe) in the lung and liver of the mice were also measured.ResultsThe activity of AST, ALT, LDH, LPO and concentrations of Mn were achieved peak value after one day of Mn administration (P<0.05) and then decreased significantly.Concentrations of Ca in the lung and liver in Mn exposed groups were significantly higher than those of in controls and achieved peak value two days after Mn administration(P<0.05),but concentrations of Fe and Zn were significantly decreased(P<0.05).Obvious toxicity of Mn to lung and liver of mice was observed.ConclusionLipid peroxidation and metalbolic obstacle of Ca, Zn and Fe may be inportant mechanism of lung and liver injury caused by Mn in mice.
Cesarean section and its influencing factors in pregnant women
SONG Bo, FANG Li-wen, ZHANG Ying
2009, 25(10): 1244-1245. doi: 10.11847/zgggws2009-25-10-48
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ObjectiveTo investig ate the status of cesarean section(CS)andits influencing factors in pregnant women of community population in Haidian district, Beijing.MethodsA total of 1 126 puerperal women without pregnancy complications who accepted postnatal carein eight community hospitals were evaluated by questionnaire from Apr.to Oct.2007.Results55.6% of pregnant outcomes were vaginal delivery and 44.4% were CS(among which 40.2% were induced by social factors).The result of regression analysis showed that the influencing factors of cesarean section were BMI ≥25.0 kg/m2,having abortion history,pregnancy weight gain>16kg,the pregnancies them selvesand their relatves refusing to choose vaginal delivery,having insufficient acquaintance with the disadvantages of cesarean section,without labor onset at the hospitalization,obtaining nodefinite suggestion of delivery mode from obstericians,childbearing during working day, neonatal weight>3 500 g.ConclusionThe cesarean section rate in pregnant women of Haidian district is in a high level and is affected by multiple factors.
Changes of synapse and differential expression of relevant genes in cerebrum of mice exposed to arsenic
SUN Jing-song, JIN Shan-shan, PIAO Feng-yuan
2009, 25(10): 1246-1248. doi: 10.11847/zgggws2009-25-10-49
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ObjectiveTo examine the influence of subchronic arsenic (As) exposure on synapse ultrastructures and expressions of relevant genes in cerebiwn of mice.MethodsThirty mice were divided into three groups including two experinental groups(1 mg/L or 4mg/L As2O3)and one control As2O3 was given for consecutive 60 days, followed by decollation Changes of synapse ultrastructure and differential expressions of the relevant genes were examined with electron microscope and Gene Chip technique.ResultsIn controls, synaptic vesicle increased obviously within presynapse in cerebrum of the mice.Compared to the controls, synaptic vesicle decreased obviously within presynapse in cerebmm of the mice exposed to As In genechip screening, 10 differential expressions of the relevant genes were observed in experin ental groups Among these genes, Cam k4, Tac1, Drd1a and Drd2 were upregulated and Cp1x2, Cacna1a, Grin2b, Gan 7, AIs2 and Gan2 were down regulated.ConclusionSubchronic exposure to As may affect adversely synapse ultrastructure and expressions of relevant genes in cerebrum of mice.These results indicate that synapse in cerebrum may be a target of As-induced neurotoxicity.
2009, 25(10): 1248-1249. doi: 10.11847/zgggws2009-25-10-50
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Influence of baicalin on tumour necrosis factor-α and interleukin-8 in rats infected with Pneumocystis carinii pneumonia
ZHOU Bi-ying, ZHOU Ling
2009, 25(10): 1250-1251. doi: 10.11847/zgggws2009-25-10-51
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ObjectiveTo study the in fluence of baicalin(Bai)on tumournecrosis factor-α(TNF-α)and interleukin-8 (IL-8)on Pneumo cystis carinii pneumonia(PCP)in rats.MethodsSprague-Dawley(SD)rats were subcutaneo usly injected with dexamethasone sodium phosphate.The treatment group was treated by baicalin(Bai)before and after the PCP model established.The pathological change in lung tissue and the level of TNF-and IL-8 in sera were observed.ResultsThe contents of TNF-α of Bai treatment group(2.14±0.14 ng/mL),low dose group(2.57±0.15 ng/mL),moderate dose group(1.46±0.14 ng/mL)and high dose group(1.12±0.13 ng/mL)were higher than those of normal control group (0.70±0.21 ng/mL,P<0.05,P<0.01)and were lower than those of model control group(3.65±0.73 ng/mL, P<0.01).The contents of TNF-of SMZ/TM Pcontrol group(1.59±0.14 pg/mL)were higher than those of normal control group(P<0.01)and were lower than those of model control group(P<0.01).The contents of IL-8 of Bai treatment group(0.43±0.11 ng/mL),low dose group(0.47±0.10 ng/mL),moderate dose group(0.39±0.11 ng/mL) and high dose group(0.32±0.11 ng/mL)were lower than those of model control group(0.65±0.12 ng/mL,P<0.01).The contents of IL-8 of SMZ/TMP control group(0.33±0.12 ng/mL)were lower than those of model control group (P<0.01).The inflammation of lung tissue was more significantly lightened in treatment group than that in model control group,and the number of foamy exudates in alveolar sacs was obviously fewer.ConclusionBai has immunological regulation effect in PCP treatment in rats and may decrease the content of TNF-and IL-8,which may lighten the inflammation of lung tissue in PCP rats.
Protective effect of zinc on oxidative injury of testis induced by chronic alcohol exposure in rats
XIE Li-jun, ZHAO Song
2009, 25(10): 1252-1254. doi: 10.11847/zgggws2009-25-10-52
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ObjectiveTo study testicular structural and functional disturbance induced by chronic alcohol exposure in rats and the protective effect of zinc against the injury.MethodsThirty healthy Sprague-Dawley adult male rats were randomly divided into 3 groups: control, alcohol (7.5g/kg) and alcohol (7.5g/kg)+zinc gluconate (7.7mg/kg).Alcohol and zinc gluconate were administmted to the rats for 13 weeks by gastric tube Speun counting, motility and the percent of abnounal speun were observed Semm sexual hounones(T) were deteun fined The pathological changes of testicle tissue were observed by light and electron microscopy The malonaldehyde (MDA)content of testicular mitochondrial was sinultaneously deteunined.The expression of NOS in testis was detected by inmunohistochemistry and western blot.ResultsCompared with the control group, the speun counting and motility of the alcohol group were decreased (P<0.05);the frequency of abnounal speun was increased(P<0.05);and the seaun T level significantly decreased (P<0.05).The MDA content and the expression of NOS were markedly higher than that of control(P<0.05).Histological evaluation of testis revealed that seminiferous epithelium was disorganized and that sertoli cells and geun cells were degenerated in alcohol-treated rats Zinc supplementation decreased the testicularm itochondrial MDA founation and the expression of NOS in testis and the degeneration of geun cells was inproved Speun counting and motilitywere higher than that of alcohol treated group.But the seaun T level declined.ConclusionChronic alcohol intake leads to speun atogenesis and steroidogenesis inhibition in testis of rats Zinc supplementation inhibits the testicularperoxidation injury.
Medical care seeking of patients with sexually transmitted infection among female sexual-workers in Yunnan province
YANG Xia, HE Chao-yang, LIU Xue-mei
2009, 25(10): 1254-1255. doi: 10.11847/zgggws2009-25-10-53
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ObjectiveTo understand the way ofmedicalcare seeking of sexually transmitted infection(STIs) patients among female sexual workers(FSWs) in Yunnan for promoting FSWs to get correct way to see a doctor.MethodsFemales engaged in commercial sexual service were selected as subjects and interviewed face to face with constructed questionnaire to collect the infounanon of medical care seeking in recent one year.ResultsAmong 798 STIs identified, 43.0% of the patients bought medicine by themselves; 24.9% went to general hospital and 17.9% selected private clinic There were significant differences in ratios of selecting general hospital between different groups of ages(χ2=11.419, P=0.010),place of sexual service(χ2=6.007, P=0.050),residence area(χ2=4.810, P=0.028) and race (χ2=4.296, P=0.038).ConclusionMost of FSWs with STIs had incorrect medical care seeking behavior and lacked of knowledge about correct medical care seeking The administmtion should adopt strict rule of medicine prescription in drugstore and private clinic.
2009, 25(10): 1256-1257. doi: 10.11847/zgggws2009-25-10-54
Abstract(831) HTML (165) PDF 241KB(65)
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2009, 25(10): 1257-1258. doi: 10.11847/zgggws2009-25-10-55
Abstract(722) HTML (130) PDF 401KB(107)
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Quality of life and related factors in middle age intellectuals
WANG Feng, DENG Bing
2009, 25(10): 1259-1260. doi: 10.11847/zgggws2009-25-10-56
Abstract(1285) HTML (160) PDF 369KB(122)
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ObjectiveTo investigate the present situation and related factors of quality of life in mfiddle age intellectuals and provide the basis of health protection.MethodsThrough stratified sampling, 1 315 persons aged from 40-60 and engaged in different profession were investigated with the self-compliled scale and WHOQOL-REF.ResultsThe quality of life in middle age intellectuals was not ideal 14.9% of persons investigated were with lower quality of life The domain with the lower and lowest score were mentality and environment.The affecting factors of quality of life were education, sleeping time, physical exercise, disease in two weeks, chronic disease, medical expenditure.The result of multifactor analysis showed that the influence factors of quality of life from strong to weak were physical exercise, two-week disease, chronic disease, medical expenditure, and education.ConclusionThe quality of life in middle age intellectuals is not ideal.The main reasons are worse mental health status, bad life environment and education.
Psychoactive substance use among university and middle school students in Guangzhou
WANG Li-jie, ZHU De-xiang, WANG De-quan
2009, 25(10): 1261-1262. doi: 10.11847/zgggws2009-25-10-57
Abstract(1248) HTML (195) PDF 407KB(74)
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ObjectiveTo collect information on psychoactive sbustance use among universtity and middle school students in Guanzhou, and to provide basis for intervention measures.MethodsA self-admroistered questionnaire survey was conducted among 4594 students from 10 universities and 8 middle schools in Guanzhou.ResultsAmong 4594 students, 26.1% ever tried cigarete smoking(one or more puffs);the prevalence of current cigarette use was 6.2%.62.4% of the students tried alchol drinking; 14.0% had accident injury after drinking; 4.3% had compound cough syrup use experience.The precentage of the students who reported illegal drug use was 1.2%.The prevalence of psychoactive substance use was higher among the male than in the female.Students who had characteristics such as loneliness, out of harmony with other person, bad performante record, and bad family environment had higher prevalence of psychoactiove substance use than other students.ConclusionThe prevalence of psychoactive substance use among university and middle school students should not be ignored.It is necessary to implement comprehensive prevetion in school, family and society.
2009, 25(10): 1263-1264. doi: 10.11847/zgggws2009-25-10-58
Abstract(1150) HTML (241) PDF 224KB(101)
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2009, 25(10): 1265-1266. doi: 10.11847/zgggws2009-25-10-59
Abstract(1106) HTML (156) PDF 241KB(68)
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Expression of group A rotavirus VP7 gene in recombined Lactobacillus
WU Jing-hua, WANG Hai-xin, ZHAO Jie
2009, 25(10): 1266-1267. doi: 10.11847/zgggws2009-25-10-60
Abstract(818) HTML (122) PDF 446KB(75)
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ObjectiveTo constmct vector pEDM 27/5-P7 by RT-PCR and transfoun it into Lacotobacillus for the construction of genetic engineering vaccine against rotavirus.MethodsThe VP7 gene fragment was isolated and recoveried by RT-PCR, and tmnsfoun edinto pEDM 27/5.The gene was transformed into Lactobacillus through extraction from the plasmid.After lactose induction, the vp7 was detected with SDS-PACE and Western-blot at 4, 8 and 12 hours.ResultsThe VP7 gene was transformed into pEDM 27/5 successfully, and the susceptibity bacteria was recombined by pEDM 27/5-vp7.After lactose induction at 4, 8, 12 hours, the protein (28KD)was detected with SDS-PACE and Western-blot.The expressed protein was 2.30%,5.12%,5.38% of total protein of the bacteria, respectively by scanning, and the expression increased with the increment of the time.The protein could combined it specially combined with anti-vp7 protein.ConclusionThe recombinant Lactobacillus expresses immunocompetent vp7 protein constantly, and could be used for further development of genetic engineering vaccine against rotavirus.
2009, 25(10): 1268-1269. doi: 10.11847/zgggws2009-25-10-61
Abstract(1036) HTML (164) PDF 384KB(56)
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Diphtheria control during different periods in Binzhou city
CAO Guo-qiang, ZHAO Yong-hua, YANG Ying
2009, 25(10): 1269-1270. doi: 10.11847/zgggws2009-25-10-62
Abstract(1036) HTML (264) PDF 237KB(75)
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ObjectiveTo evaluate the effect of diphtheria control during different periods in Binzhou city.MethodsDescriptive epidemiology was adopted for analysis of the epidemic situation of diph the ria,the vaccination rate of diphtheria toxoid vaccine,the population mimunity level, and control effects from 1951 through 2008.ResultsSince the planned mimunity was implemented,the vaccination rate of diphtheria toxoid vaccine was kept at 93.45-95.67%;the mimune successful rate and the diphther ia antibody level were kept at 93.63-94.30% and 89.40-89.54%.A total of 34186 ineidences and 2325 death cases were reduced in the past 38 years since the diphther ia toxoid vaccine was regularly vaccinated in 1970 with the annual average reduction of 900 incidences and 6.1 deaths.No diphtheria case occured since 1978.ConclusionVaccina ting diphtheria toxoid vaccine is one of the comprehensive measures of diphtheria prevention.The diphtheria incidence is controlled effectively in Binzhou city.
2009, 25(10): 1271-1271. doi: 10.11847/zgggws2009-25-10-63
Abstract(1041) HTML (155) PDF 231KB(111)
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2009, 25(10): 1272-1272. doi: 10.11847/zgggws2009-25-10-64
Abstract(1116) HTML (143) PDF 127KB(119)
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2009, 25(10): 1273-1275. doi: 10.11847/zgggws2009-25-10-65
Abstract(866) HTML (186) PDF 318KB(111)
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2009, 25(10): 1277-1279. doi: 10.11847/zgggws2009-25-10-67
Abstract(1057) HTML (155) PDF 255KB(82)
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Unhealthy dietary behavior in diabetes mellitus patients
YAN Ya-geng, DONG Feng-li, ZHANG Lin
2009, 25(10): 1279-1280. doi: 10.11847/zgggws2009-25-10-68
Abstract(983) HTML (150) PDF 522KB(123)
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ObjectiveTo describe unhealthy dietary behaviors in diabetes mellitus (DM)patients related to the age, weight, occupation, education for health education in DM patients.MethodsThe unhealthy dietary behaviors were categeorized into irregular diet, drinking, salty taste, and fast eating.The infoun anon was collected with interview.ResultsThe prevalence rate of irregulart diet in the patients aged between 40-49 years was 37.59% and the prevalence rate of alcohol drinking was 35.94%.The rate of the salty taste and faster eating in the patients aged between 50-59 was 34.45 and 34.43%,respectively.The proportions of fast eating, salty teste and alcohol drinking were higher in the patients with BMI>24 than those of other groups.There was no significant correlation between irregular diet and body weight in DM patients The proportions of fast eating, salty teste, and alcohol drinking in cardres were higher than those of patients with other occupanon (P<0.05).The patients with moderate education had higherprevalences of irregular diet, alcohol drinking, and fast eating than patients with lower or higher education.ConclusionThe prevalences of the unhealthy dietary behavior vary among DM patients and health education should be promoted among the patients.
论文
Knowledge related to breast cancer and its influencing factors among women
LIU Li-yuan, JIA Cun-xian, YU Zhi-gang
2009, 25(10): 1275-1276. doi: 10.11847/zgggws2009-25-10-66
Abstract(1436) HTML (172) PDF 517KB(148)
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ObjectiveTo understand the status of knowledge related to breast cancer and its influencing factors among women in Gaomicity for health education of breast cancer in the population.MethodsWith randomized cluster sampling, across-sectional study was conducted to collect information of women aged 25 to 70 years in Gaomi.ResultsFor 7 850 women inves tegaed,only 15 12% had higher awareness level1 85.8% of the women were aware of breast cancer,75.4% knew that the screening could detect breast cancer early and 77.6% knew the screening could increase survival rate.The breast cancer screening rate among the women was 43.4%.The six factors related to high cognition of breast cancer were age (OR=0.63,95% CI=0.692-0.843),education(OR=1.545,95% CI=1.385-1.723),living area(OR=1.210,95% CI=1.022-1.434),worker(OR=1.928,95% CI=1.543-2.410),other occupation(OR=21028,95% CI=1.420-2.897), medical care personnel(OR=9.828,95% CI=7.316-13.536).ConclusionThere is a considerable inadequacy in breast cancer konwledge among the women in Gaomi,and breast cancer screening rate in this population is low.