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2012 Vol. 28, No. 7

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Influence of social resources on incidence of MCI and its subtypes among the elderly
CAI Yi-yuan, HUANG Wen-yong, YANG Jing-yuan
2012, 28(7): 881-883. doi: 10.11847/zgggws-2012-28-07-01
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Objective To analyze the effect of social resources,including social network and social activity,on mild cognitive impairment(MCI)and its subtypes in community elderly of Guiyang city. Methods A total of 2 144 persons aged 60 years and older with normal cognitive function and social resources was followed up with questionnaire and scale surveys in Guiyang city.The influences of social resources on the incidence of MCI and its subtypes were analyzed with Cox regression model. Results At the beginning the survey,there were 2 144 participants and at the end of the survey,the number of participants was 1 401,of wihic there were 102 new MCI(71 amnesic MCI and 31 nonamnesic MCI).The incidence density of MCI was 15.21/1 000 person years and those of amnesic MCI and nonamnesic MCI were 10.59/1 000 person years and 4.62/1 000 person years.In the 1 401 respondents,the number of elders contacting with their children, relatives,friends,and neighbors were 822(58.7%),782(55.8%),898(64.1%),and 956(68.2%),respectively.There were 937(66.9%)elders married and lived with others.The number of the elders with poor,less,moderate,and abundant social resource was 52(3.7%),283(20.2%),673(48.0%),and 392(28.0%),respectively.There were 180(12.9%) elders not taking part in congregate activity,227(16.2%)elders taking part in intellectual activity,807(57.6%)elders taking part in physical activity,and 187(13.3%)taking part in other activities.There were 501(35.8%)elders having activity occasionally and 719(51.3%)elders having activity usually.Keeping contact with children and friends were protective factors for amnestic MCI(relative risk[RR]children=0.510,95% confidence interval[CI]:0.317-0.820;P=0.005;RRfriends=0.611, 95% CI:0.380-0.982;P=0.042).There was no influence on the incidence of MCI subtypes for other social resources. Conclusion The results show that adequate social resources are helpful to decrease the incidence of amnestic MCI.
Ability of daily living and its influencing factors among rural elderly in Dabieshan Mountain area
HUANG Wei, GE Xiao-jiao, HOU Sai
2012, 28(7): 884-886. doi: 10.11847/zgggws-2012-28-07-02
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Objective To examine the status of activities of daily living(ADL)and its influencing factors among rural elderly population in the Dabieshan Mountain area. Methods Totally 1 117 rural elderly aged 60 years and older were chosen with cluster sampling from a town of Dabieshan Mountain area and then their ADL was measured with a self-designed questionnaire and the influencing factors of ADL were analyzed with multinomial logistic regression. Results The total number of valid questionnaires was 1 117(353 for male and 764 for female).The mean age of the elderly was 70.17±6.624 years and the mean score of ADL was 19.37±7.097.The prevalence rate of reduced ADL in the elderly was 73.1%.The results of univariate analyses showed that age,marital status,chronic disease,and living condition could influence ADL in the eledrly(P<0.05).The results of logistic regression analysis revealed that age, economic control,whether with chronic disease,physical training,and living condition affected ADL of the elderly(P<0.05 for all). Conclusion The reduced ADL among rural elderly in Dabieshan Mountain area was prevalent and living condition and quality of life of the elderly should be improved.Age,physical training,economic control,whether with chronic disease,and living condition were significant predicting variables of ADL.
Reliability and validity of SF-36 in health survey among empty-nest elders in countryside
ZHAO Hua-shuo, WANG Ke, JIN Ying-liang
2012, 28(7): 887-889. doi: 10.11847/zgggws-2012-28-07-03
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Objective To access the reliability and validity of 36-Item Short Form Health Survey(SF-36)in the evaluation of quality of life among the empty-nest elders in countryside. Methods Totally 218 empty-nest elders were selected with stratified cluster random sampling for a face-to-face interview with SF-36.The reliability of SF-36 was evaluated by split-half reliability and internal consistency reliability and its validity was evaluated by discriminant validity, convergent validity,and structure validity. Results The split-half reliability was 0.84 and the internal consistency Cronbach'α coefficient was 0.89.The Cronbach's α coefficient for role-physical,physical functioning,bodily pain, general health,vitality,social funtioning,role-emotional,and mental health was 0.79,0.88,0.86,0.81,0.77,0.76,0.88, and 0.77,resectively.The convergent validity was 97.14% and discriminant validity was 98.21%.By confirmatory factor analysis,we got a model,of which the structure was consistent with the theoretical assumption,and two main statistics of root mean square error of approximation(0.12)and confirmatory fit index(0.91). Conclusion The results indicate that the SF-36 is a useful scale for the evaluation of the quality of life among the empty-nest elders in countryside.
Relationship between life habits and mild cognitive impairment:a case-control study
YIN Shu-qin, NIE Hong-wei, XU Yong
2012, 28(7): 890-892. doi: 10.11847/zgggws-2012-28-07-04
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Objective To examine the relationship between mild cognitive impairment(MCT)and life habits,and to provide evidence for intervention on the disease. Methods Stratified random cluster sampling was used and a casecontrol study including 117 MCI patients and 117 cognitive normal controls was conducted. Results The results of univariate analysis and multivariate analysis were consistent.Physical exercise,healthy diet,consumption of health products, doing housework,and hobby were significantly related to MCI. Conclusion Physical exercise,healthy diet,consumption of health products,doing housework and hobby are protective factor of MCI.
Anti-oxidant function of tea leaves on D-galactose-induced senescent mice
CHEN Xue, YI You-jin, LIU Hua-jin
2012, 28(7): 892-894. doi: 10.11847/zgggws-2012-28-07-05
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Objective To investigate the effect of different types of fermented tea leaves on D-galactose-induced senile mice. Methods Seventy male mice were randomly divided into control group,D-galactose control group,green tea group,oolong tea group,black and dark tea group,and vitamin C(vit C)group.The oxidant damage model was established by consecutively injecting D-galactose into the hypoderm of the mice for 30 days.At the same time,the mice in four kinds tea groups and vit C group were administered orally with tea solution(2 g/kg·bw·d)and vit C(10 mg/kg·bw·d). Thirty days after the treatments,the mice were sacrificed and four organ-indexes were examined and the activities of super oxide dismutase(SOD),catalase(CAT)and total anti-oxidative capacity(T-AOC)in serum,liver and brain tissue of the mice were measured. Results The organ-indexes for liver,brain,spleen,and thymus gland in green tea group were higher than those of D-galactose-induced aging mice model group(P<0.05).In the oolong tea group,only the contents of SOD(5.51±0.66 U/mL),T-AOC(14.87±0.57 U/mL)in serum increased(P<0.05).The contents of SOD,CAT and T-AOC in serum,liver and brain tissue in green,black,and dark tea group were increased(P<0.05).The antioxidant effect of the green tea was the strongest and that of the black and dark tea were similar. Conclusion Different types of fermented tea leaves possess strong anti-oxidant effects on D-galactose-induced oxidative damage in vivo and the effect of green tea is the strongest.
Risk factors for type 2 diabetes mellitus among community residents in Harbin city
MIAO Chang-qing, YAN Hong-hai, WANG Li-ping
2012, 28(7): 895-897. doi: 10.11847/zgggws-2012-28-07-06
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Objective To evaluate the risk factors of type 2 diabetes mellitus(T2DM)in community residents of Harbin city and to provide scientific basis for making appropriate prevention and intervention measures. Methods With cluster random sampling method,1 491 residents from 4 communities in Harbin municipality were investigated with a questionnaire survey,physical examination and laboratory detection. Results A total of 1 491 people including 456 people with T2DM were surveyed and the prevalence of T2DM was 30.58%.The prevalence rates of T2DM were significantly different among the residents of different age,education level,different status of family history of diabetes,hypertension,overweight or obesity,abdominal obesity,high blood lipids,exercise and alcohol drinking(P<0.05 for all). The results of unconditional multivariate logistic regression indicated that age,family history of diabetes,high blood lipids,abdominal obesity,hypertension and overweight or obesity were risk factors for T2DM among the residents. Conclusion Age,family history of diabetes,high blood lipids,hypertension and obesity are major risk factors for T2DM among community residents in Harbin city.
Prevalence and risk factors of main chronic diseases in rural residents of Yiling district,Yichang city
WANG Le-qun, ZHANG Fan, ZHANG Gui-lin
2012, 28(7): 898-902. doi: 10.11847/zgggws-2012-28-07-07
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Objective To examine the prevalence of main chronic diseases and its risk factors among rural population in Yiling district,Yichang city. Methods Using stratified cluster sampling,9 781 migrants and non-migrants aged from 35 to 75 years in Yiling district,Yichang city were investigated with a questionnaire survey,physical examination, and laboratory test. Results The number and prevalence of hypertension,diabetes mellitus,dyslipidemia,and obesity were 3 677(37.6%),340(3.4%),2 801(28.3%),and 629(6.3%),respectively.The number and the age-adjusted prevalences of the diseases were 1 617(39.3%),139(3.3%),1 127(27.9%),and 224(5.7%)in men and 2 060 (36.6%),201(3.5%),1 674(29.0%),and 405(7.0%)in women,respectively.The rate of hypertension in men was higher than that in women(P<0.05),but the prevalence of obesity among women was higher than among men (P<0.05).Compared to non-migrants,the rate of dyslipidemia among migrants was lower,but the rate of obesity was higher(P<0.05).Multivariate logistic regression analyses showed that the related risk factors of hypertension and diabetes mellitus included family history,with the odds ratio(OR)and 95% confidence interval(CI)of 2.114(1.8932.360)for hypertension and 4.229(2.705-6.611)for diabetes mellitus. Conclusion Hypertension and diabetes are main chronic diseases and intervention strategies should be taken among the residents of Yiling district of Yichang city.
Status and influencing factors of adequate health literacy among occupational populations in rural areas of China
YAN Li-ping, WEI Nan-fang, AN Jia-ao
2012, 28(7): 902-905. doi: 10.11847/zgggws-2012-28-07-08
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Objective To investigate the adequate health literacy(AHL)status and to analyze its influencing factors in rural occupational populations in China,and to provide scientific evidence for developing intervention strategies of health literacy. Methods The investigation covered 32 987 workers in rural areas aged 15-69 years selected from all provinces,autonomous regions,and municipalities of mainland China with multistage stratified random sampling method. A face-to-face questionnaire survey was conducted among the workers. Results The proportion of adequate health literacy (PAHL)among the rural workers was 3.98%.The proportion of the workers with AHL in mental,light physical labor, agriculture workers,and farmers was 16.90%,16.20%,2.14%,and 4.50% respectively.The proportion of AHL for health concept and know ledge,lifestyle and behavior,and related skills among the workers was 10.14%,4.09%,and 13.05%,respectively.In addition,the proportion of AHL was 23.35%,11.35%,2.77%,12.33%,and 4.76%,respectively,for the follow ing health issues:attitude tow ards health,safety and first aid,infectious diseases prevention,basic medical care,and chronic non-communicable diseases prevention.logistic regression analysis results indicated that education(odds ratio[OR]=0.497),occupation(OR=-0.222),nationality(OR=-0.175),region(OR=-0.1670.071),age(OR=-0.094),and marriage(OR=0.049)were important influencing factors of AHL level in rural occupational populations.The educational level was positively correlated with the proportion of PAHL.PAHL was lower among elder people and the minority.The PAHL in the workers in central China was higher than that in the eastern China and PAHL was lowest in the workers of the Western. Conclusion Education,occupation,nationality,region,age,and marriage are important influence factors of health literacy and special intervention measures should be focused on these factors to improve health literacy among the rural occupational populations.
Association between single nucleotide polymorphism of tryptophan hydroxylase isoform(TPH2) gene rs4570625 and depression and suicidal behavior
WANG Lin, YANG Yan-Jie, MA Jing-song
2012, 28(7): 906-908. doi: 10.11847/zgggws-2012-28-07-09
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Objective To explore the relationship between single nucleotide polymorphism(SPN)of a novel tryptophan hydroxylase isoform(TPH2)gene rs4570625 and depression and suicidal behavior. Methods We collected the specimens of peripheral blood from 300 uniplor depression patients and 300 healthy controls in a mental hospital in Harbin city.The amplification of novel tryptophan hydroxylase isoform(TPH2)gene rs4570625 was executed by polymerase chain reaction(PCR)and analyzed with direct sequencing.The association between the polymorphisms of TPH2 gene and depression and suicidal behavior was analyzed with SPSS 11.0. Results The genotypic frequencies of the SNP didn't deviate from Hardy-Weinberg equilibrium in both case and control groups(P>0.05).Compared with the control group, no significant difference in genotypes and alleles of TPH2 gene rs4570625 was found in the patient group(P>0.05). Meanw hile there was no significant correlation between genotypes of TPH2 gene rs4570625 and suicide behavior in patient group.There were significant differences in T,G frequencies of TPH2 gene rs4570625 between the patients with and without suicidal behavior(56.5% vs 47.6%,43.5% vs 52.4%;P<0.05 for all). Conclusion TPH2 gene rs4570625 SNP may have no association with the susceptibility of depression.
Distribution of gamma-glutamyl transferase and its correlated factors in rural men of Yanbian area:a cross-sectional study
JIN Yong-zhe, PIAO Xian-nü, XIONG Ying-huan
2012, 28(7): 909-912. doi: 10.11847/zgggws-2012-28-07-10
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Objective To investigate the distribution of gamma-glutamyl transferase(GGT)and its correlated factors in rural men of Yanbian area. Methods A cross-sectional study was carried out in 2 001 residents aged 30-70 years. Results The level of the natural logarithm GGT among Korean men(3.55±0.86)was significantly higher than among Han men(3.27±0.72,F=69.366;P<0.000),and the prevalence of GGT disorder in Korean men(25.5%, 260/1 018)was significantly higher than in Han men(13.2%,[130/983],χMH2=51.408;P<0.000).Single factor analyses revealed that age(χk2=18.788,P<0.01),education level(χk2=8.387,P<0.01),drinking and smoking (χk趋势2=33.876,P<0.000;χH趋势2=30.527,P<0.000),hypertension(χk2=13.922,P<0.000;χH2=16.629, P<0.000),isolated diastolic hypertension(IDH)(χk2=9.363,P<0.01;χH2=8.219,P<0.01)and systolic hypertension (SDH)(χk2=11.276,P<0.01;χH2=14.422,P<0.000)were correlated factors for GGT disorder;multiple factor analyses showed that nationality(Korean),age,alcohol drinking,hypertension,SDH,IDH,and isolated systolic hypertension (ISH)were correlated factors for GGT disorder(P<0.05),and a dose-response relationship was observed between GGT disorder and alcohol drinking. Conclusion The prevalence of GGT disorder had significant ethnic difference.The prevalence of GGT disorder was positively correlated with age,alcohol drinking,hypertension,SDH,IDH,and ISH(P<0.05) among men in the area,and dose-response relationship was sobserved between GGT disorder and alcohol drinking.
Correlation of serum sIL-2R and IL-2 levels with life event and coping style in depression patients
CHEN Jun-yu, ZHANG Gui-qing, HU Min
2012, 28(7): 912-914. doi: 10.11847/zgggws-2012-28-07-11
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Objective To analyze the correlations of soluble interleukin 2 receptor(sIL-2R)and interleukin 2 (IL-2)serum levels with life event and coping style in depression patients. Methods Sixty drug-naive depressed patients were recruited and assessed with Life Event Scale(LES),Coping Style Questionnaire,and Haniltion Depression Scale(HAMD).sIL-2R and IL-2 levels were evaluated using enzyme-linked immunosorbent assay(ELISA)method in the patients and 60 healthy controls. Results The drug-naive depression patients had significantly lower serum IL-2 level than control group(P=0.025).The coping style and the score of LES were not correlated with sIL-2R and IL-2 levels among the patients(P>0.05).Compared with the controls,the patients experienced more stressful life events and used more immature coping styles(P<0.05). Conclusion There may be a decreased serum IL-2 level for depression patients.The levels of serum sIL-2R and IL-2 were not correlated with the score of life event and coping style.
Risk factors of hand-foot-mouth disease among children in Beijing city
JIA Lei, YANG Jun-yong, GUO Jing
2012, 28(7): 915-917. doi: 10.11847/zgggws-2012-28-07-12
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Objective To explore the risk factors of hand-foot-mouth disease(HFMD)in Beijing city and to provide reference for prevention of the disease. Methods A unified questionnaire was used to survey the innate immunity factors and acquired factors,including rearing pattern and living standard,related to HFMD.Statistical analyses were conducted with SPSS 13.0. Results No associations were observed between HFMD and innate immunity.Exposed to individuals with suspected symptoms within 10 days before the medical attendance(odds ratio[OR]=2.692,P<0.05)was a risk factor.Healthy habits(OR=0.763,P=0.055),having been to hospital within 10 days before the hospitalization (OR=0.587,P=0.005),rearing cats at home(OR=0.339,P=0.035)and having larger living space(20-39 m2/per person:OR=0.466,P<0.05;≥40m2/per person:OR=0.554,P=0.005)were protective factors.There were 62.85% of HFMD cases had been exposed to individuals with suspected symptoms. Conclusion Execpt the isolation of the children with HFMD,improvments of health habits and medical attendance consciousness in vulnerable children in lowincome family,living in bungalow or limited living space are benificial to prevent HFMD in Beijing.
Acceptability of male circumcision among men who have sex with men in Guiyang city
ZHENG Min, LIU Min, YAO Yong-ming
2012, 28(7): 918-920. doi: 10.11847/zgggws-2012-28-07-13
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Objective To investigate the acceptability of male circumcision(MC)and its influencing factors among men who have sex with men(MSM)in Guiyang municipality to promote the acceptability of male circumcision for the prevention of sexually transmitted diseases. Methods Questionnaire interviews were administered to 357 MSM recruited and the results of 302 MSM without MC experience were analyszed. Results Among the MSM,57.3% (173/302)had the first sex at the average age of 21.7 years and 61.6% (186/302)had the first sex with men.For the MSM the average number of male partners in the latest 6 months was 4.2 and 94.4% (285/302)of MSM had anal sex with men but only 31.4% (89/302)used a condom everytime.There were 17.9% (54/302)of MSM having anal sex with female,of which only 35.1% (19/54)used a condom everytime.Totally 48.01% (145/302)of the MSM accepted MC.Multivariate logistic regression analyses showed the influencing factors included knowing the effect of MC on prevention of sexually transmitted diseases(P=0.016),with self-diagnosed prepuce or phimosis(P=0.006),awareness of effect of MC on quality of sex(P=0.000),and believing in good quality of MC(P=0.000). Conclusion The knowledge about effects of MC on sexual function and prevention of sexually transmitted diseases are related to the acceptability on MC among MSM in Guiyang city.
Status and influencing factors of attitude toward LGBT among college students
ZHANG Pei-chao, CHI Xin-li, WU Ming-xia
2012, 28(7): 921-923. doi: 10.11847/zgggws-2012-28-07-14
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Objective To explore the status and influencing factors of attitude toward lesbian,gay,bisexality,and transgender(LGGT)among college stuldents and to provide implication for sex education in the students. Methods Totlly 1 762 college students fom 5 universities in Chongqing and Chengdu city were selected for the questionnaire survey. Results There were 219 students(16.5%)having homosexual attraction themselves,154(8.5%)reporting having homosexual contact,and 74(4.2%)reporting gender variant identity.Among the students,the percentage of reporting the acceptance was 12.4% (218)for having intercourse with the same sex,12.6% (222)for having romantic love with transgender,16.8% (207)for changing sex,19.1% (347)for having sex drive toward the same gender, 26.2% (461)for homosexuality marriage,28.9% (510)for bisexuality being accordant to social norms,and 35.1% (618)for homosexuality being accordant to social norms. Conclusion Chinese college students have negative and conservative attitude tow ard LGBT,which could be influenced by gender,grade,residence and parents'education background,rather than influenced by sex education experience,discipline and family structure.Besides,gender is an important moderate factor for the impact of parents'education level and residence on the attitude toward LGBT among college students.
Effects of parental working out-of-home on health risk behavior and psychological status among left-hehind children
LI Guang-you, LUO Tai-min, TAO Fang-biao
2012, 28(7): 924-926. doi: 10.11847/zgggws-2012-28-07-15
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Objective To examine the impacts of parental working out-of-home on behavior and mental status among left-behind children. Methods A questionnaire survey about adolescent health risk behavior and psychological status was conducted among 2 139 pupils(810 left-lehind children under age of 14 years)from 2 full-time schools.Results For the 810 left-behind children,the number of children with their fathers,mother,and both parents working out-ofhome were 334(42.47%),146(18.02%),and 320(39.51%),respectively.The ratio of smoking hehaviors among the children with their mothers working out-of-home was significantly higher than those with their father or both parents working out-of-home(8.22% vs 3.8%,χ2=9.117,P=0.003;8.22% vs 3.23%,χ2=10.504,P=0.001).The ratio of alcohol drinking once a week or more among the children with both their father and mother woking out-ot-home was obviously higher than those with father working out-of-home and those without parents working out side.(11.25% vs 6.4%,χ2=4.850,P=0.003;11.25% vs 7.67%,χ2=4.315,P=0.038).The children with their mother working outof-home had a higher ratio of fighting at least once in a term or more than those of with father working out-of-home and thsoe of without parents working out-of-home(9.57% vs 4.65%,χ2=4.312,P=0.0038;9.59% vs 6.42%, χ2=4.127,P=0.042).The children without parents working out-of-home had a lower ratio of internet surfing compared to thsoe with both parents working out-of-home(17.8% vs 28.44%,χ2=24.283,P=0.000).The results of unconditional logistic regression revealed that the influencing factors of smoking behavior were age,grade,and gender(female) with the odds ratio of 1.129,0.646,and 0.146,respectively. Conclusion Working out-of-home of hoth father and mother,especially the working out-of-home of mother,has impacts on health risk behavior and mental status of leftbehind children.
Relationship between obesity index of National Physical Fitness Evaluation Standard and hypertension risk
HAN Yan-bai, WANG Hong-li, CHEN Tao
2012, 28(7): 927-930. doi: 10.11847/zgggws-2012-28-07-16
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Objective To evaluate the relationship between obesity index and hypertension risk and to identify the cut-off point for the prevention of hypertension. Methods Stratified cluster random sampling method was used to select a total of 5 809 adults aged 20-69 years from three settings of the Third China's National Physical Fitness Surveillance of Liaoning province including Shenyang,Dandong and Chaoyang city.Physical examination was conducted for the subjects without antihypertensive medications. Results Among the 5 809 subjects,the prevalence of high normal blood pressure and hypertension was 16.92% and 24.89%,respectively.The prevalences of high normal blood pressure and hypertension in men(20.98%,31.57%)were significantly higher than those in women(12.94%,18.33%)(P<0.01).Multivariate logistic regression results showed that older than 40 years and body mass index(BMI)were risk factors,and female gender was a protective factor for high normal blood pressure.For hypertension,older than 30 years,BMI and waist circumference were risk factors,and female gender was a protective factor.After adjusted for age and gender,risks of high normal blood pressure and hypertension increased by 1.38(95% confidence interval[CI]:1.28-1.50)and 1.57 (95% CI:1.39-1.78)times with the increase of per standard deviation of BMI.The risk of hypertension increased by 1.37(95% CI:1.20-1.56)times with the increase of per standard deviation of waist circumference.Based on the maximal Youden's index,the cut-off points of BMI to identify high normal blood pressure were 24.0 kg/m2 for men and 24.3 kg/m2 for women,and the cut-off points to identify hypertension were 25.1 kg/m2 for men and 24.3 kg/m2 for women.The cut-off points of waist circumference to identify hypertension were 87.0 cm for men and 80.6 cm for women.On the basis of sensitivity higher than 80%,the cut-off points of BMI to identify high normal blood pressure were 23.0 kg/m2 for men and 22.3 kg/m2 for women,and the cut-off points of BMI to identify hypertension were 23.4 kg/m2 for men and 22.8 kg/m2 for women.The cut-off points of waist circumference to identify hypertension were 82.0 cm for men and 76.9 cm for women. Conclusion Risk of hypertension increases with the increments of BMI and waist circumference,and BMI is more strongly associated with hypertension than waist circumference.
Evaluation of multiple intervention on psychological health level among train staff
TANG Shi-xiong, ZHONG Shao-ling, PAN Jie
2012, 28(7): 930-932. doi: 10.11847/zgggws-2012-28-07-17
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Objective To study the effect of psychological intervention on psychological health level of the train staff and to provide reference for the prevention of railway traffic accident. Methods Psychological health status of 323 train staff in intervention group and 403 in control group from Liuzhou locomotive terminal was tested with Symptom Checklist-90(SCL-90).Individual therapy,group therapy,health education,and psychological hotline were applied to the staff in the intervention group based on their total scores of SCL-90. Results There were 108 staff with scores of SCL90 >160 and the average was 153.92±48.89 for the staff in the intervention group.There were 152 staff with the scores of >160 and the average score of SCL-90 was 160.09±55.45 for the staff in the control group.The detection rates of psychological problems for the intervention and control group were 33.40% and 37.71% and all of the factor scores of SCL-90 of the staff were higher than those of national norm(P<0.01).After the intervention,the 8 factor scores of SCL-90 for the staff in the intervention group showed a 8% decrease with an average score of 144.86±35.42 (P<0.05 for all)except somatic symptom(P<0.05 for all).Compared with those of the control group,nine symptom factor scores for the intervention group were lower(P<0.05).Compared with the survey data of the staff in Guangzhou Railway Group,all factor scores of SCL-90 for the staff were lower(P<0.05 for all)except the factors of obsession and paranoia. Conclusion The psychological health of the train staff is lower than that of the national norm.The psychological intervention could improve overall psychological health among the train staff.
Role of Nrf 2 signal pathway in lead-induced oxidative stress in SH-SY5Y cells
LI Ping, LI Fen, YE Fang
2012, 28(7): 933-935. doi: 10.11847/zgggws-2012-28-07-18
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Objective To investigate the role of NF-E2-related factor 2(Nrf 2)signal pathway in lead-induced oxidative stress in SH-SY5Y cells as well as the possible mechanism. Methods SH-SY5Y cells were exposed to 0,5,25, and 125μmol/L lead acetate for 24 hours.After harvesting the cells,the level of reactive oxygen species(ROS)was measured by the method of 2',7'-dicholorofluorescin diacetate(DCFH-DA),and glutathione(GSH)was tested by dithiothymine double-nitrobenzonic acid(DTNB)method.Western blot was used to detect the levels of protein kinase C-theta(PKC-δ),casein kinase 2(CKⅡ)Nrf 2 in the cytoplasm and nucleus. Results Compared with the control group,the level of ROS of the moderate and high dose group were obviously increased(P<0.05),while the level of GSH in all groups obviously decreased(P<0.01).Compared with the control group,the protein expression level of Nrf 2 in the cytoplasm were significantly decreased(P<0.05).Meanwhile the protein expression levels of Nrf 2 in the nucleus of the high dose group showed a distinct elevatation,which was remarkbly different from that of the control group(P<0.05). Compared with the control group,the protein expression level of PKC-δ was significantly increased(P<0.05).The protein expression level of CKⅡof the high dose group showed a significant elevatation(P<0.05). Conclusion The findings demonstrate that lead can induce a nuclear accumulation of the transcription factor Nrf 2,which indicates the mediation of Nrf 2 in the cellular response against the oxidative stress caused by lead.The results also indicate that PKC-δ and CKⅡ play certain role in the Nrf 2 activation through increasing expression levels of two proteins stimulated by lead.
Gene polymorphism of CYP1B1 Leu 432 Val in Guangxi Zhuang population
LIAO Chang-xiu, LI Shu-bo, TANG Wei-dong
2012, 28(7): 936-938. doi: 10.11847/zgggws-2012-28-07-19
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Objective To investigate hereditary character of cytochrome P450 1B1(CYP1B1)codon 432 among Guangxi Zhuang population. Methods Gene polymorphism of CYP1B1 codon 432 was detected by di-allele-specificamplification with artificially modified primers(diASA-AMP)in 288 healthy Guangxi Zhuang people.The genotype frequencies of CYP1B1 codon 432 in the population were compared with other populations in China,other countries and regions. Results The frequency of allele C in CYP1B1 gene Leu 432 Val was high in both female(89.1%)and male population(91.4%).The prevalence rates of CYP1B1 Leu 432 Val genotypes CC,CG,and GG were 78.3%,21.3%, and 0 in the female population,and those in male population were 83.6%,15.6%,and 0.8%,respectively.There was no difference between female and male population in allele and genotype frequency of CYP1B1 Leu 432 Val in the population.The frequency of genotype distribution in the population had no obvious differences compared with Korea popoulation,but had significant differences compared with other populations in Jiangsu,Shanghai,Sichuan(P<0.05),Japan, India,Poland,Nigeria,and America(P<0.01). Conclusion Gene polymorphism of CYP1B1 in codon 432 has no gender difference in Guangxi Zhuang population,and the distributions of the gene polymorphism in the population are different from those in other regions of China and other countries.
Association between polymorphism of microsatellite D6S289,D6S1610 and schizophrenia
ZHANG Jie, YUAN Ye-feng, WAN Fu-sheng
2012, 28(7): 939-942. doi: 10.11847/zgggws-2012-28-07-20
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Objective To investigate the association between genetic polymorphism of microsatellite D6S289, D6S1610 and schizophrenia in Han population in Jiangxi province. Methods Genomic DNA was isolated from the blood samples of 306 schizophrenia patients from Jiangxi Psychiatric Hospital and 248 healthy persons taking physical examination in Jiangxi Province Chinese Medicine Hospital.The association between D6S289,D6S1610 microsatellite loci and schizophrenia was analyzed. Results Of the healthy control group,the observed values and expected values of D6S289 and D6S1610 alleles were consistent with Hardy-Weinberg equilibrium.Detection rate of D6S289-213 bp in schizophrenia group was 8.3%,and 0.0% in the control group,with significantly different gene frequency(χ2=39.854, P=0.000),indicatiing that D6S289-213 bp might be a schizophrenia risk gene.Detection rate of D6S289-215 bp in schizophrenia group was 5.7% and 11.8% in the control group,with significanlty different gene frequency (χ2=12.320,P=0.000;odds ratio[OR]=0.45),indicating that D6S289-215 bp might be a schizophrenia protective gene.Detection rate of D6S289-223 bp in schizophrenia group was 3.6% and 6.6% in the control group,with significantly different gene frequency(χ2=4.661,P=0.031,OR=0.54),suggesting that D6S289-223 bp might be a schizophrenia protective gene.Detection rate of D6S1610-127 in schizophrenia group was 32.4% and 38.5% in the control group,with significantly different gene frequency(χ2=4.296,P=0.038,OR=0.76),suggesting that the gene might be a schizophrenia protective gene.Detection rate of D6S1610-131 bp in schizophrenia group was 1.5% and 5.4% in the control group,with signifecantly different gene frequency(χ2=13.160,P=0.000,OR=0.26),suggesting that the gene might be a schizophrenia protective gene.Detection rate of D6S1610-133 in schizophrenia group was 28.4% and 21.6% in the control group,with significantly different gene frequency(χ2=6.270,P=0.012,OR=1.44),suggesting that the gene might be a schizophrenia risk gene.The detection rate of D6S1610-137 bp was 4.4% in schizophrenia group and 0.7% in the control group,with significantly different gene frequency(χ2=13.013,P=0.000,OR=6.55), suggesting that the gene may be a risk gene of schizophrenia.Other allele frequencies between the two groups were not significantly different(P>0.05). Conclusion Gene susceptibility to schizophrenia of Han population in Jiangxi province may exist near the microsatellite loci D6S289 and D6S1610 in chromosome 6.
Effect of subchronic arsenic exposure on concentrations of cerebellar monoamine neurotransmitters and preventive roles of taurine and vitamin C in mice
WANG Ya-chen, PIAO Feng-yuan, MA Ning
2012, 28(7): 942-944. doi: 10.11847/zgggws-2012-28-07-21
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Objective To examine the effect of subchronic exposure to arsenic(As)on levels of three monoamine neurotransmitters in brains of mice and to provide evidences for exploring mechanism of As-induced neurotoxicity. Methods Seventy Kunming mice were randomly divided into one control group(drinking water alone),three experimental groups(1,2 and 4 ppm As2O3),and two protection groups(4 ppm As2O3+150 mg/kg taurine and 4 ppm As2O3+45 mg/kg vitamin C[Vit C]).As2O3 was given through drinking water for 60 days.The mice were decapitated and their brains were removed.Three monoamine neurotransmitters,including onrepinephrine(NE),dopanline(DA),and 5-hydroxy tryptamine(5-HT)in cerebellum of mice were measured by high-performance liquid chromatography(HPLC) with fluorescence detector. Results The concentrations of NE,DA,and 5-HT were 188.96 ng/g,45.30 ng/g,and 148.42 ng/g in the mice cerebellum of control group.The concentrations of NE,DA,and 5-HT in mice cerebellum were 92.65,12.36 ng/g,and 147.26 ng/g and 37.18 ng/g,9.44 ng/g,and 105.00 ng/g7 and 22.35 ng/g,7.40 ng/g,and 65.58 ng/g,respectively,in the 1,2,and 4 ppm As2O3 groups.Levels of these neurotransmitters were significantly lower in the cerebellum of the mice exposed to As than those in the controls(P<0.05).On the contrary,the concentrations of NE,DA,and 5-HT were 30.76 ng/g,7.23 ng/g,and 77.88 ng/g and 37.75 ng/g,12.54 ng/g,and 61.41 ng/g in the taurine and Vit C group,being significantly higher than those of the group exposed to As(P<0.05). Conclusion Subchronic exposure to As can decrease the concentrations of 5-HT,DA,and NE in cerebellum of mice.Co-administration of taurine or Vit C may protect against the decreased concentrations of monoamine neurotransmitters induced by As.
Lung tissue ultrastructure and hypoxia-inducible factor 1 alpha expression of lung tissue in rats exposed to high altitude hypoxia
LI Wen-hua, YUAN Dong-ya, SUN Fang-yun
2012, 28(7): 945-948. doi: 10.11847/zgggws-2012-28-07-22
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Objective To examine the effects of high altitude hypoxia on ultrastructure and hypoxia-inducible factor 1α(HIF-1α)expression of lung tissue in the rats with high altitude hypoxia exposure. Methods Forty SD rats were exposed to high altitude hypoxia for 1,2,3,and 30 days,respectively,and a control group(10 rats)was set.The 4 altitude hypoxia groups were transported from the place of 5 m above sea level to the place of 2 700 m above sea level within one hour,to the place of 5 000 m above sea level within 2 hours,and to the place of 4 500 within 3 hours and raised at the place for 30 days. Results Light microscopy and electron microscopy showed that in 2 days acute high altitude hypoxia group there was apparent high altitude pulmonary edema.For the rats with high altitude hypoxia of 30 days,HIF-1α mRNA band integral absorbance values(0.874±0.075)was significantly higher compared with the control group (0.225±0.026,P<0.01)and high-altitude pulmonary edema was significantly reduced. Conclusion Hypoxic acclimatization could increase the expression of hypoxia-inducible factor 1αmRNA and reduce the high altitude pulmonary edema of lung tissue in rats.
Influence of hyperhomocysteinemia on learning and memory ability and expression of β-amyloid precursor protein of hippocampus and intervention effect of folic acid in rats
WANG Jian, ZHANG Yong-ze, KANG Mei-yu
2012, 28(7): 948-950. doi: 10.11847/zgggws-2012-28-07-23
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Objective To explore the influence of hyperhomocysteinemia(HHcy)on learning and memory ability and expression of β-amyloid precursor protein of hippocampus and the intervention effect of folic acid(FA)in rats. Methods Forty Wistar rats were randomly divided into normal control group,HHcy group,and two FA intervention groups(low and high dose).Methionine(1 g/kg·d)was dissolved in drinking water to make HHcy model.Treatment with FA(0.7 and 3.4 mg/kg·d)via intragastric intubation was administered in FA groups for 8 weeks.Plasma concentrations of homocysteinemia(Hcy)and FA before and after the experiment were measured.The rats'learning and memory abilities were tested by Morris water maze test.At the end of 8 weeks experiment,immunohistochemistry was used to observe the expression of β-arnyloid precusor protein(APP)in hippocampus. Results Compared with the normal control and FA groups,the escape latency in HHcy group was significantly longer;the numbers of passing the platform region was significantly less;the staying time in the platform region was significantly shorter(P<0.05-0.01);and the mean values of APP and its metabolic secretase in region of hippocampus was significantly higher(P<0.01).The results of all tests were not significantly different between the normal control group and FA groups. Conclusion HHcy can induce learning and memory impairment and decreased the expression of APP in hippocampus in rats.FA supplementation could attenuate the adverse effects.
Effect of tea polyphenols on neurotoxicity induced by methylmercury in rats
LIU Wei, XU Zhao-fa, DENG Yu
2012, 28(7): 951-953. doi: 10.11847/zgggws-2012-28-07-24
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Objective To examine the effect of tea polyphenols on toxic effect induced by methylmercury. Methods Thirty-six Wistar rats were randomly divided into 3 groups by weight.The first group was control group;the second group was treated with 12μmol/kg CH3HgCl;the third group was the tea polyphenols pretreatment group.The pretreatment and CH3HgCl treatment lasted for 4 weeks.Twenty four hours after the last treatment,the activities of Na+-K+-ATPase and Ca2+-ATPase,the contents of Ca2+ and reactive oxygen species,the levels of apoptosis in pallium were determined. Results Compared with the control group,the activities of Na+-K+-ATPase(2.72±0.46μmol/[h·mg]) and Ca2+-ATPase(1.52±0.26μmol/[h·mg])decreased significantly(P<0.01)and the content of Ca2+ (239.52±44.84 nmol/L),the content of reactive oxygen species(313.86±35.11 fluorescence intensity),and the level of apoptosis(40.84±6.26%)increased significantly(P<0.01 for all)in CH3HgCl treatment group.Compared with CH3HgCl treatment group,the activities of Na+-K+-ATPase(3.58±0.71μmol/[h·mg])and Ca2+-ATPase (1.98±0.29μmol/[h·mg])increased significantly(P<0.05,P<0.01)and the content of Ca2+(188.39±7.43 nmol/L),the content of reactive oxygen species(238.03±22.99 fluorescence intensity)and the level of apoptosis (28.31±4.34%)decreased obviously in the tea polyphenols pretreatment group(P<0.05,P<0.01,and P<0.01). Conclusion Tea polyphenols has a protective effect on the neurotoxicity induced by methylmercury in rats.
2012, 28(7): 953-955. doi: 10.11847/zgggws-2012-28-07-25
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Relationship between Big Five personality and happiness index in breast cancer patients
NING Nan, YANG Yan-jie, QIU Xiao-hui
2012, 28(7): 956-957. doi: 10.11847/zgggws-2012-28-07-26
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Objective To explore the relationship between personality traits and well-being among breast cancer patients and to provide basis to improve the quality of life of breast cancer patients. Methods A total of 180 breast cancer patients and 180 healthy female cotrols were selected at Affiliated Tumor Hospital and Physical Examination Center of Harbin Medical University in October 2011 for a questionnaire survey with Big Five Personality Inventory and a happiness index scale to evaluate the personality characteristics and the happiness index of the subjects. Results The Big Five personality scores of adaptability and sense of morality of the cancer patients were 46.52±12.31 and 56.51±6.67 and significantly higher than those of the controls(42.70±10.76 and 44.72±8.53;P<0.05);the scores of sociability, openness of the patients were 54.78±9.31,43.49±11.22 and obviously lower than those of the controls(62.24±9.28, 60.08±11.76;P<0.05).Breast cancer patients had a happiness index score of 9.35±2.47,significantly lower than that of the controls(12.82±1.46;P<0.05).Openness,altruism and happiness index were positively correlated among breast cancer patients(r=0.232,P<0.05;r=0.256,P<0.05). Conclusion Personality traits is one of the factors that affect the well-being of breast cancer patients.
Relationships between weight-for-height of National Physical Fitness Evaluation Standard and metabolic syndrome
WANG Hong-li, HAN Yan-bai, ZHAO Ze-lin
2012, 28(7): 958-960. doi: 10.11847/zgggws-2012-28-07-27
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Objective To investigate the relationship between weight-for-height and metabolic syndrome(MS). Methods Physical examination was performed among 1280 staff aged 30-69 years in Northeastern University.Severe underweight,underweight,normal weight,overweight,and severe overweight groups were difined according to the National Physical Fitness Evaluation Standard. Results The prevalence of MS was 31.8%,and the prevalence in men(36.2%) was significantly higher than in the women(24.0%)(χ2=20.24,P<0.01),and the prevalence of MS in the overweight (48.1%)and severe overweight groups(60.2%)were significantly higher than that in the normal weight group (23.5%)(χ2=40.94,χ2=109.40;all P<0.01).In the normal weight group,the prevalence of MS increased with increasing age,and the prevalence of MS was at high level in the subjects older than 50 years.Meanw hile,in the normal weight group,the prevalences of abdominal obesity(42.3%),hypertension(56.0%),hyperglycemia(30.0%),and hypertriglyceridaemia(29.9%)were all at high level.The risks of MS in the subjects with normal weight and abnormal waist circumference were 12.58(95% confidence interval:8.14-19.42;P<0.01)times higher compared to those with both normal weight and normal waist circumference. Conclusion The prevalence of MS is high in the subjects with normal weight-for-height.Weight-for-height should be combined with waist circumference in order to identify MS at early stage.
Molecular epidemiology of hand,foot and mouth disease in Tianjin
YANG Dong-jing, LI Li
2012, 28(7): 961-964. doi: 10.11847/zgggws-2012-28-07-28
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Objective To analyze the pathogens of hand,foot,and mouth disease(HFMD)and the molecular epidemiology of enterovirus 71(EV71)in Tianjin. Methods A total of 2 377 clinical specimens from HFMD patients in Tianjin,2008-2010,were tested by real-time reverse transcription-PCR(RT-PCR).EV71 strains were isolated from some EV71-positive specimens.VP1 genes of the EV71 isolates were amplified by RT-PCR and sequenced.Phylogenetic tree was constructed after homology analysis of VP1 genes. Results Total positive rate of pan-enteroviruses was 70.72% (1 681/2 377)in the HFMD cases.Among the cases,48.30% were positive for EV71,37.36% for CV-A16,and 14.34% for other enterovirus cases.The thirty-one EV71 isolates had the highest homology(95.7%-99.2%)with the reference strain of C4a cluster of the C4 subgenotype.Phylogenetic analysis of entire VP1 gene of the EV71 isolates showed that they belong to C4a cluster of the C4 subgenotype. Conclusion EV71 was the principal pathogen of HFMD in Tianjin,2008-2010.The epidemic strains were C4a viruses of the C4 subgenotype.
Family influencing factors of behavior problems among primary school students in Mudanjiang city
SUN Jia-si, CAI Jing-yu, WANG Lie
2012, 28(7): 964-966. doi: 10.11847/zgggws-2012-28-07-29
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Objective To explore family influencing factors of the behavior problems among primary school students. Methods Questionaire research was conducted with Achenbach's Child Behavior Check List(CBCL)and Egma Minnen av Bardndosnauppforstran(EMBU)among 1 350 students at 4-6 grade in primary schools in Mudanjiang city. Results The prevalence of behavior problems in the students was 19.3% (20.3% in boys,18.6% in girls).The scores of somato-complaints,anxiety-depression,social intercourse problems,aggressive behavior,thinking problems, introversion behavior,and total behavior scores were 2.50±2.89,3.73±3.96,2.54±2.81,4.31±4.73,2.17±3.00, 8.34±8.15,and 22.03±20.07,respectively,in the students,which were higher than those of the norms.The results of correlation analysis showed that parents'warming attitudes were inversely related to the students'behavior problems (P<0.01),whereas,fathers'punishment,interpose,partialism,rejection,overprotection and mothers'over protection, rejection,punishment,partialism were positively related to the students'behavior problems(P<0.01).In stepw ise regression analysis,the personality characteristics of mother rejection(β=0.206),life events(β=0.195),relationship with parents(β=0.133),father overprotection(β=0.124),and only child family(β=0.083)were significantly associated with behavior problems in the students. Conclusion Familial environment factors have an important influence on the incident of children behavior problems and improving familial environment may play an important role to decrease the prevalence of behavior problems amang primary school students.
Surveillance of viral encephalitis in some areas of Henan province
TANG Xiao-yan, LI Xing-le, KANG Kai
2012, 28(7): 967-970. doi: 10.11847/zgggws-2012-28-07-30
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Objective To analyze pathogen species and prevalent characteristics of the viral encephalitis in some areas of Henan province. Methods Surveillance data on all viral encephalitis(VE)cases diagnosed in 2008 were collected from Xinxiang,Kaifeng,and Luoyang.The blood and cerebrospinal fluid(CSF)samples were collected from the cases and IgM antibody against Japanese encephalitis(JE)was detected with enzyme-linked immunosorbent assay(ELISA).The antibody of other virus was detected with reverse transciption-PCR(RT-PCR).Cell culture separation was conducted for IgM antibody negative specimens. Results A total of 215 viral encephalitis cases were reported and 47(21.86%)cases were positive for IgM antibody against JE.A total of 24(14.29%)intestinal virus strains were isolated from the cerebrospinal fluid of 168 IgM antibody negative cases.Among the the virus,there were twelve of ECHO30,seven of ECHO6, four of ECHO25,and one of CoxB5.The 47 JE and 168 intestinal virus and other viral encephalitis cases occurred mainly in July to September,accounting for 93.62% and 70.24% of the cases.The 0-14 years old group of JE and intestinal virus and other virus encephalitis cases occurred mainly in Xinxiang(91.91% and 92.31%)and Kaifeng(97.92% and 98.41%).The 0-14 years old cases of intestinal virus and other viral encephalitis cases accounted for 92.99% of all cases in Luoyang city and≥15 years old cases of JE significantly increased(56.52%).The incidences of JE,intestinal virus,and other viral encephalitis were significantly different between 0-14 years old group and≥15 years old group (χ2=21.13,P<0.05).The JE cases were mainly farmers in Luoyang and the JE cases of intestinal virus and other viral encephalitis in Xinxiang and Kaifeng were mostly scattered children,children in and kindergarten,or students. Conclusion Japanese encephalitis virus and intestinal virus are main pathogens of viral encephalitis in Xinxiang, Kaifeng,and Luoyang.The peak season of the intestinal virus and other viral encephalitis was the same as that of JE.The age and occupation distribution of the cases are different in different areas.
Relationship between changes of diameter and shape of yolk sac and early abortion
JIN Su-juan, YE Ping, HUANG Bin
2012, 28(7): 970-972. doi: 10.11847/zgggws-2012-28-07-31
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Objective To explore the relationship between diameter and shape of yolk sac(YC)and early spontaneous abortion. Methods Totally 517 cases of singleton pregnant women were detected by abdominal ultrasound and were followed up.All cases were divided into the normal pregnancy group,threatened abortion group and spontaneous abortion group according to the pregnancy outcomes.The size and shape of yolk sac,the inner diameter of gestational sac were recorded. Results Ultrasound could detected the yolk sac at 5 weeks of pregnancy.The inner diameter of gestational sac was the largest at 9 week and then the diameter was narrowed and disappeared at 12 week.The changes of inner diameter of the gestational sac showed a square relation to gestational time with a regression equation of Y=2.290+0.09X0.001X2 and a coefficient of R2=0.634(P=0.000).There were 10 cases with no yolk sac detected,32 cases with the size less than normal,11 cases with normal size,9 cases with the size larger than normal,7 cases with deformation,7 cases with calcification,and 3 cases with abnormal echo.The anomalies of shape and size of yolk sac for threatened abortion and spontaneous abortion group were different and the difference was statistically significant(P<0.05).logistic regression analysis showed that the detection of the yolk sac,the size and shape of yolk sac were independent risk factors for abortion. Conclusion The size and shape of yolk sac were closely related to the pregnancy outcomes.The yolk sac detection by ultrasound at the early stage of pregnancy was a predictor of pregnancy outcome.
Prevalence of overweight among adults in Liaoning province from 1991 to 2006
LI Sui-jing, LI Xin, LIU Zhong-mei
2012, 28(7): 973-975. doi: 10.11847/zgggws-2012-28-07-32
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Objective To investigate the prevalence and trend of change in overweight among adults in Liaoning province from 1991 to 2006. Methods The study was based on the data collected in China Health and Nutrition Survey in 1991,1993,2000,2004,and 2006 and the subjects were healthy adults. Results During the period of 1991-2006,the prevalence of overweight among the adults increased from 24.4% to 48.8%,with an increase rate of 100% and an accelerated increase rate.The prevalence of overweight was higher in the men than in the women and the difference in the prevalence between the men and the women decreased gradually.The subjects aged 18-30 years had the highest increase rate of overweight.Among the urban subjects,the men had a higher increase rate but the women had a higher increase rate among rural subjects. Conclusion The prevalence of overweight increased significantly with a 100% increment among the adults in Liaoning province from 1991-2006.
2012, 28(7): 975-976. doi: 10.11847/zgggws-2012-28-07-33
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KAP about AIDS among college students
PAN Yan, XU Zhao-qiang, LI Ce
2012, 28(7): 977-979. doi: 10.11847/zgggws-2012-28-07-34
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Objective To examine the knowledge,attitude,and behavior(KAP)about AIDS among college students,and to provide evidence for health education on AIDS. Methods With a self-designed questionnaire,an investigation about KAP on AIDS was carried out among 814 students in colleges. Results Of all the students,82.7% (673/814)thought that AIDS was not very far away from them and 59.6% (485/814)could exactly remember the date of World AIDS Day but 27.5% (224/814)did not know the day.There were 74.6% (607/814)of the students willing to have class with AIDS students in the same classroom and 49.8% (405/814)willing to have dinner with AIDS patients. There were 92.6% (754/814)of the students considered that AIDS patients should get sympathy and help from the public.The proportion of sources for AIDS know ledge from teachers,friends and classmates,and special lectures was 46.8% (381/814),27.9% (227/814),and 20.9% (170/814),respectively among the students. Conclusion The college students have the sense of AIDS risk but are lack of self-protection aw areness and accessible AIDS know ledge in the colleges.
2012, 28(7): 979-980. doi: 10.11847/zgggws-2012-28-07-35
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2012, 28(7): 982-985. doi: 10.11847/zgggws-2012-28-07-37
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Preparation of Lpp20-GST fusion protein of Helicobacter pylori with thrombin-cleavage of GST tag on column
JIANG Yin, WANG Xiao-ping, HE Dian-dian
2012, 28(7): 987-989. doi: 10.11847/zgggws-2012-28-07-39
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Objective To express Lpp20-GST fusion protein with glutathione-S-transferase(GST)fusion gene expression system and the cleavage of GST-tag on glutathione sepharose 4B column using thrombin. Methods The recombinant expression plasmid Lpp20/pGEX4T-1 was induced in E.coli BL21(DE3)by isoproythio β D-galacoside (IPTG)and the bacterial sediment was lysed by repeating freezing and thawing,lysozyme lysis,and ultrasonic wave.The soluble supernatant was loaded on glutathione sepharose 4B column and GST-tag was cleavaged on column using thrombin.Purified Lpp20 was proved by mouse anti-Lpp20 monoclonal antibody(mAb)with western blot. Results The fusion protein Lpp20-GST was partly expressed in soluble form with relative molecular mass of 45 kDa.Thrombin cleavaged GST-tag on column and purified Lpp20 was recognized by mouse anti-Lpp20 mAb. Conclusion Target protein can be obtained by thrombin-cleavage of GST-tag on column.
2012, 28(7): 999-1003. doi: 10.11847/zgggws-2012-28-07-43
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2012, 28(7): 981-982. doi: 10.11847/zgggws-2012-28-07-36
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2012, 28(7): 986-986. doi: 10.11847/zgggws-2012-28-07-38
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2012, 28(7): 990-991. doi: 10.11847/zgggws-2012-28-07-40
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2012, 28(7): 992-994. doi: 10.11847/zgggws-2012-28-07-41
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2012, 28(7): 994-998. doi: 10.11847/zgggws-2012-28-07-42
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2012, 28(7): 1003-1008. doi: 10.11847/zgggws-2012-28-07-44
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