幽门螺杆菌与白细胞介素-1基因交互作用

廖娜; 段广才; 郗园林; 白雪飞

幽门螺杆菌与白细胞介素-1基因交互作用

Interaction between Helicobacter pylori and interleukin-1 gene

摘要

摘要:
目的探讨幽门螺杆菌(Hp)感染与白细胞介素-1基因家族中白细胞介素-1B(IL-1B)-31与IL-1B-511(IL-1B)位点单核苷酸多态性(SNP)、IL-1RN可变数目串连重复序列(VNTR)及二者交互作用与十二指肠溃疡病(DU)发病风险的关系.
方法用病例对照研究方法, 调查94例对照和54例DU患者, ELISA方法检测患者IgG抗体, PCR扩增目的基因, 电泳检测IL-1RN可变数目串连重复序列, 限制性片段多态性分析(RFLP)检测IL-1B-31、IL-1B-511单核苷酸多态性.
结果 Hp感染I、L-1B-511 C/C型增加DU的患病危险, OR分别为8.26(95% CI=3.02~22.59), 3.05(95% CI=1.23~7.57).Hp感染和IL-1B-31T/TI、L-1B-511C/C、非Ⅰ型IL-1RN之间有交互作用存在, 交互作用项OR分别为16.75(95% CI=3.64~77.04), 41.42(95% CI=8.96~191.40), 4.93(95% CI=1.35~17.99), 交互作用系数(Υ)分别为1.22, 1.7, 0.84.
结论 IL-1B-31 T/T、IL-1B-511 C/C能增加Hp感染对十二指肠溃疡病患病的危险作用, 而非Ⅰ型IL-1RN则减弱Hp的危险作用.这种交互作用的存在有助于解释Hp感染临床结局的复杂性.

Abstract:
Objective To elucidate relations among H. pylori infection and interleukin-1 locus polymorphisms:IL-1B-31, IL-1B-511 and IL-1RNand to investigate interaction of both in development of duodenal ulcers.
Methods Casecontrol study was carried out involving 94 control subjects and 54 patients with duodenal ulcer.H. pylori states was examined with ELISA, VNTRof IL-1RN was examined with PCR, PCR-RFLP was used to analyze SNPs of IL-1B-31 and IL-1B-511.
Results Helicobacter pylori infection, carriage of IL-1B-511 C/C increased risk of duodenal ulcer with odds ratios of 8.26(95% CI=3.02-22.59)and 3.05(95% CI=1, 1.23-7.57)respectively.Odd ratios of interaction between H. pylori infection and IL-1B-31T/T, IL-1B-511C/C, non-IL-1RN were 16.75(95% CI=3.64-77.04), 41.42(95% CI=8.96-191.40), 4.93(95% CI=1.35-17.99), and γ was 1.22, 1.7, 0.84 respectively.
Conclusion H. pylori infection and IL-1B-31T/T, IL-1B-511 C/C synerg istically determine susceptibility to duodenal ulcer, IL-1RN can reduce the risk effect of H. pylori infection.The interaction is helpful to explain the complexity of H. pylor-iasso-ciated disease.

HTML全文

参考文献(7)

施引文献

资源附件(0)