Abstract:
Objective To study the protection effect of metal net-like defense cloth in vivo and genistein on J urkat cell after electromanetic pulse(EMP).
Methods Wistar rats and Jurkat cells were exposed to high power EMP of 6×104 V/m as experimental models.By various methods such as biochemical tests, light microscopy, methyl thiazolyl tetrazolium(MTT), flow cutometry, the protection effect of metal net-like defense cloth and protein tyrosine kinase(PTK)inhibitor Genistein on immune organization and cell were investigsted.
Results (1) Peripheral blood lymphocyte of rats sheltered with metal net-like defense cloth(EM-4)recovered, more quickly than that of rats unsheltered; (2)Pretreatment with gensitein could partially block the degression of EMP on the proliferation activity and Jirkat cell in S phsase instantly.
Conclusion (EM-4) and PTK inhibitor Genistein are effective to protect the immune function, cell proliferation activity and DNA synthesis from EMP radiation.