褐藻糖胶对人白血病细胞株K562凋亡的影响
Effect of fucoidan on human leukaemia tumor cell line K562 apoptosis
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摘要:目的 探讨海带提取物褐藻糖胶对体外培养的人髓系白血病细胞株K562生长的影响, 从细胞凋亡角度研究其抗肿瘤机制。方法 采用肿瘤细胞体外培养、单细胞凝胶电泳、凋亡细胞DNA琼脂糖凝胶电泳和流式细胞技术, 观察不同剂量褐藻糖胶对体外培养的白血病细胞株K562细胞的影响。结果 噻唑蓝(MTT)实验结果显示, 褐藻糖胶对K562细胞均具有较强的杀伤作用, 尤其以1 g/L剂量组为明显。单细胞凝胶电泳实验结果表明, 褐藻糖胶对肿瘤细胞DNA有明显损伤作用, 尤其以1 g/L剂量组的影响最为明显。DNA琼脂糖凝胶电泳和流式细胞仪检测结果显示, 经褐藻糖胶处理后, 肿瘤细胞凋亡率上升, 其中1 g/L剂量组较为明显。提示褐藻糖胶可能通过诱导肿瘤细胞凋亡而发挥抗肿瘤作用。结论 褐藻糖胶可通过诱导肿瘤细胞凋亡, 从而达到抗肿瘤的目的。Abstract:Objective To study anti-tumor mechanism of fucoidan on human leukaemia tumor line K562.Methods The human leukaemia tumor cell lines were cultured in vitro. The effect of fucoidan to K562 was investigated by the single cell gel electrophoresis (SCGE), DNA ladder and FACS techniques after treated by water extract or ethanol extract from Laminaria japonica Aresch with doses of 0.5, 1, 2 and 3 mg/ml.Results Experimental study of MTT was used to determine the antitumor effects of fucoidan on inducing apoptosis of tumor cell, the dose of 1 mg/ml was remarkable. SCGE was used to detect the breakage of DNA strand and analyze damage of fucoidan to tumor cell. The result showed the damage was evident. The DNA ladder and FACS techniques showed the incidence rates of apoptosis were remarkably increased after treated 24 hours by fucoidan. The dose of 1 mg/ml increased significantly.Conclusion The anti-tumor effect of fucoidan was evident, and its mechanisms was probably involved the apoptosis of tumor cell which induced by fucoidan.
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