大蒜油和白藜芦醇联合应用诱导胃癌细胞凋亡

郑国华; 李颢; 李会庆

大蒜油和白藜芦醇联合应用诱导胃癌细胞凋亡

Study on garlic oil combined resveratrol inducing apoptosis of human gastric carcinoma cell line MGC-803

摘要

摘要:
目的探讨大蒜油和白藜芦醇联合用药诱导胃癌MGC-803细胞凋亡作用。
方法噻唑蓝(MTT)法测药物对细胞增殖抑制; 应用吉姆萨染色、流式细胞术、激光共聚焦扫描显微镜等方法观察药物作用后细胞凋亡情况。
结果大蒜油和白藜芦醇联合用药6, 12, 24 h明显抑制胃癌细胞增殖, 抑制率分别为42.8%, 59.8%和65.5%。用药24 h后, 吉姆萨和原位末端标记(TUNEL)染色可见明显的细胞凋亡; 流式细胞术分析联合用药组早期细胞凋亡数为35.83%, 大于两者单独用药之和; 激光共聚焦扫描显微镜显示联合用药组DNA含量明显低于对照组和单独用药组。
结论大蒜油和白藜芦醇联合用药诱导胃癌MGC-803细胞凋亡效应比单独应用明显增强。

Abstract:
Objective To explore the effect of garlic oil combining with resveratrol inducing apoptosis of human gastric carcinoma cell line MGC-803.
Methods To measure the inhibit effect of proliferation on garlic oil combining with resveratrol to gastric carcinoma cell by MTT. Apoptotic effect of gastric carcinoma cell by giemsa staining was observed. TUNEL assay, flow cytometry and confocal microscopy were used.
Results The inhabiting rate of 42.8%, 59.8% and 65.5% could be measured after being exposed to garlic oil combining with resveratrol at the dosage 25μg/ml for 6h, 12h and 24h. Obvious cell apoptosis could be observed by giemsa staining and TUNNEL staining after exposed to combining medicine at 25 μg/ml for 24h. Positive cells of Annexin Ⅴ in group of garlic oil combined with resveratrol were higher than those of total positive cells of both garlic oil group and resveratrol group. The reverse results could be observed about DNA content within cells of the combined medicine group and the single medicine group by confocal microscopy.
Conclusion The effect of apoptosis on garlic oil combined with resveratrol to MGC-803 cell line is obviously stronger than that of their single usage.

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