Abstract:
Objective To screen rapidly the teratogenic contaminants which were extracted from raw drinking water and tap water in Hefei city. An in vitro short-term test was carried out.
Methods Organic contaminants were extracted from drinking water by use of XAD-4 macroreticular resins. With cultures of rat embryo limb bud cells for 5 days, the concentration inhibiting differentiation by 50% control(ID50)and the concentration inhibiting proliferation by 50% control(IP50)and the ratio of IP50/ID50 were gotten respectively by each inhibition curve.
Results The extractives inhibited the differentiation and the proliferation of limb bud cells. The ID50 for tap water were 363. 1ml/ml(-S9)and 5.7 ml/ml(+S9), IP50 were 1355.2 ml/ml(-S9)and 1264.5 ml/ml(+S9), the ratios for IP50/ID50(-S9)were 3.7, IP50(+S9); IP50 were 223;The ID50 for raw water were 1.2ml/ml(-S9)and 0.387 ml/ml(+ S9); IP50 were 15.8 ml/ml(-S9)and 41.1 ml/ml(+S9), the ratios for IP50/ID50 were 13.5(-S9)and 106.2(+S\-9). That's to say, the extractives could be bioactivated into stronger inhibitors.
Conclusion There parameters indicated the influence of organic contaminants on differentiation of rat limb bud cells was stronger than on proliferation, and those organics in drinking water belonged to a teratogenic agent and a specific inhibittors. The chlorination of drinking water should be studied more extently.
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