八氯二丙醚对小鼠肝肺细胞DNA损伤的影响

Effect of octachlorodipropylether on DNA damage of liver and lung cells in mice

摘要:
目的研究八氯二丙醚对小鼠肝、肺细胞的遗传性作用。
方法采用改进的单细胞凝胶电泳技术, 检测八氯二丙醚对小鼠肝、脾和肺细胞的DNA损伤。在小鼠接触0, 100, 200, 400, 800 mg/(kgºbw)的八氯二丙醚后0, 3, 9, 24, 48, 72 h, 从单细胞水平观察其对小鼠肝、肺等主要脏器细胞DNA损伤程度。
结果小鼠接触400, 800 mg/(kgºbw)的八氯二丙醚3, 9和24 h, 可引起明显的肝和肺细胞的DNA链断裂。但48 h后基本恢复到对照组水平。
结论八氯二丙醚在一定剂量下对小鼠具有遗传毒性作用。

Abstract:
Objective To study the genetic toxic effect of octachlorodipropylether on mice liver and lung cells.
Methods In an in vivo study, mice were sacrificed at 0, 3, 9, 24, 48 and 72 h after oral0, 100, 200, 400 and 800 mg/(kgºbw)administration of octachlorodipropylether. DNA damage was measured by the single cell gel electrophoresis(SCGE)assay.
Results After oral administration400 and 800 mg/(kgºbw), DNA damage appeard in the liver at 9, 24 h, and in the lung at 9, 24 and 48 h.
Conclusion A certain dose octachlorodipropylether has genetic toxic effect on mice.