铅致小鼠睾丸DNA损伤与细胞增殖

Study on DNA damage and proliferation of testicular cells with lead exposed

摘要:
目的研究慢性铅染毒对小鼠睾丸细胞毒性机制。
方法应用单细胞凝胶电泳技术(SCGE), 检测铅对雄性小鼠生殖细胞DNA损伤, 四甲基偶氮噻唑蓝(MTT)实验检测睾丸细胞增殖功能, 生化试剂检测细胞脂质过氧化损伤。
结果从低剂量组到高剂量组铅均可引起睾丸生殖细胞DNA不同程度损伤; 低(0.15%)、中(0.3%)、高(0.6%)DNA迁移长度及迁移率与对照组之间差异有统计学意义(P < 0.01);中、高浓度铅具有抑制睾丸细胞增殖的作用, 使睾丸丙二醛(MDA)升高、超氧化物歧化酶(SOD)、谷胱苷肽过氧化物酶(GSH-Px)活性降低。
结论铅可致睾丸细胞脂质过氧化损伤, 导致DNA链断裂损伤, 且具有抑制睾丸细胞增殖作用。

Abstract:
Objective To study the toxicity mechanism of mice testicular cells by lead exposure.
Methods
Methods of SCGE (Single cell gel electrophoresis technique)and MTT were used.
Results There were different degree DNA damage of testicular cells by lead exposed to group(0.15%), group(0.3%), group(0.6%). The length of DNA migration were significant different to the control groups(P < 0.01). Lead restrained testicular cells proliferation. MDA level were increased and SOD and GSH activity were decreased.
Conclusion Lead caused oxidative damage and DNA break damage of mice testicular cells, and restrained testicular cells proliferation.