啤酒中牛磺酸的荧光测定及稳定性研究
Study on determination of taurine and its stability in beer by fluorophotometry
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摘要:目的 探讨啤酒中牛磺酸的荧光测定方法以及牛磺酸在啤酒中的稳定性。方法 以啤酒为基质, 牛磺酸为添加剂, 分别配制成5个浓度水平牛磺酸啤酒, 各分装2份。1份放于4℃保存, 另1份置棕色试剂瓶室温保存, 放置6个月后与同样方法现配置的牛磺酸啤酒进行平行对照测定; 待测样品经去泡、离心等处理后, 采用强酸性阳离子交换柱分离出牛磺酸, 与邻苯二甲醛(OPA)/2-巯基乙醇衍生化后进行荧光法测定。结果 该方法相对标准偏差≤2.4%, 回收率 > 95%, 最小检出限为0.023 mg/L; 放置6个月的和现配置样品中的牛磺酸含量差异无统计学意义(P > 0.05)。结论 该方法快速、简便而且重现性好, 可用于测定啤酒中添加的牛磺酸; 牛磺酸在啤酒基质中非常稳定, 具备开发牛磺酸保健啤酒的可行性。Abstract:Objective To determine the content of taurine in beer by fluorophotometry and its stability in beer.Methods The normal beer was incorporated with taurine at five levels and kept under 4℃ and normal temperature respectively for use. Freshly incorporate beer was made six months later and was determined with the formerly incorporate beers all together. After pretreatment, the sample was separated and cleaned up with cation ex change column, then taurine was derived by o-phthalaldehyde and was monitored by fluorophotometry.Results T he fluorophotometry for taurine analysis in beer showed the precision(relative standard deviation)was less than 2.4%, the recoveries were more than 95%and the detection limit was 0.023 mg/L; The differences of taurine content among all the samples were not statistically significant(P > 0.05).Conclusion The method is rapid, simple, with a good reproducibility and is suitable of analysis of taurine in beer; the good stability of taurine in beer ensures the feasibility of taurine-contained hygienical beer.
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