饮食诱导雄性肥胖大鼠生长激素轴变化

郑琳琳; 姚兴家; 吴辉; 王艳; 翟玲玲; 白英龙; 刘玉芬

饮食诱导雄性肥胖大鼠生长激素轴变化

Study on change of GH/IGF-I axis in diet-induced male obese rats

摘要

摘要:
目的探讨饮食诱导雄性肥胖大鼠生长激素(GH) 轴〔GH/胰岛素样生长因子-I(IGF-I)〕的轴性变化。
方法建立高脂饮食诱导肥胖的大鼠模型, 实验12周末处死大鼠, 采用放射免疫分析方法检测大鼠血清GH、IGF-I、胰岛素水平, 用免疫组化方法检测肝脏IGF-I的含量。
结果饮食诱导肥胖后高脂组大鼠血清GH水平下降(P < 0.05);游离IGF-I水平升高(P < 0.01);血清胰岛素水平升高(P < 0.05);肝脏IGF-I的积分光密度值和阳性面积百分比均显著低于对照组(P < 0.01), 肝脏的IGF-I合成分泌下降。
结论在饮食诱导肥胖的雄性大鼠体内, 可能并不存在真正意义上的GH/IGF-I轴性变化损伤。

Abstract:
Objective To study the change of GH/IGF-I axis in diet-induced male obese rats.
Methods The rats were made obese model by high-fat diet.All rats were put to death at the twelfth weekend.The lelvels of serum GH, free IGF-I and insulin in rats were determined by radioimmunoassay.The concentrations of IGF-I in the livers were detected by immunohistochemistry.
Results The level of serum GH was lower in high-fat diet group than that of controls(P < 0.05);the level of serum free IGF-I was higher in high fat diet group than that of controls(P < 0.01), and the level of serum insulin was higher in high fat diet group than that of controls(P < 0.05);High-fat diet rats showed a significantly decrease in the integrate OD total and threshold area% of hepatic IGF-I compared with control rats(P < 0.01), IGF-I se cretion in obese rats livers decreased.
Conclusion There might be no GH/IGF-I axis impaired in real sense in diet-induced obese male rats

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