高级检索
徐健, 颜崇淮, 余晓刚, 杜学良, 吴真, 沈晓明. 铅对海马神经元单环磷酸腺苷水平的影响[J]. 中国公共卫生, 2005, 21(3): 277-278.
引用本文: 徐健, 颜崇淮, 余晓刚, 杜学良, 吴真, 沈晓明. 铅对海马神经元单环磷酸腺苷水平的影响[J]. 中国公共卫生, 2005, 21(3): 277-278.
shu
XU Jian, Yan Chonghuai, YU Xiaogang, . Effect of lead on cyclic AMP level in cultured hippocampal neuron[J]. Chinese Journal of Public Health, 2005, 21(3): 277-278.
Citation: XU Jian, Yan Chonghuai, YU Xiaogang, . Effect of lead on cyclic AMP level in cultured hippocampal neuron[J]. Chinese Journal of Public Health, 2005, 21(3): 277-278.
shu

铅对海马神经元单环磷酸腺苷水平的影响

Effect of lead on cyclic AMP level in cultured hippocampal neuron

    摘要
  • 摘要:
      目的   通过放射免疫的方法, 研究铅暴露对体外培养的海马神经元单环磷酸腺苷(cyclic adenosine monophosphatec, cAMP)水平的影响。
      方法   在体外培养3d的原代胚鼠海马神经元中, 加入不同浓度的氯化铅(分别为10-4mol/L、10-6mol/L、10-8mol/LPb2+)暴露21d, 收集海马细胞并超声粉碎后, 用放射免疫的方法测海马细胞cAMP水平。
      结果   铅抑制体外培养的海马神经元腺苷酸环化酶活性, 降低cAMP水平。1×10-8mol/L Pb2+暴露即能抑制海马细胞腺苷酸环化酶活性。铅暴露程度越高, cAMP降低越明显, 两者成负相关。
      结论   铅暴露后海马神经元cAMP水平下降可能是铅影响学习记忆的机制之一。

     

    Abstract:
      Objective   To determine the effect of lead exposure on cyclic AMP level in cultured hippocampal neurons.
      Methods   To establish the neuron model of lead exposure in vitro.After primary embryonal rathippocampal neurons were cultured for 3 days, cells were induced by different concentration of Pb2+ (10-4, 10-6, 10-8 mol/L)for three weeks.To shat ter hippocampal neurons with ultrasonic disintegrator and to determine the cyclic AMP level by radio-immunity assay.
      Results   Lead could inhibit adenylate cyclase, even in lead exposure concentration of 1×10-8mol/L.Higher level of lead exposureled to lowercyclic AMP level, which showed a dose-dependent effect.
      Conclusion   The descent of cyclic AMP level in hippocampal neur ons exposed to lead maybe one mechanism for lead affecting learning and memory.

     

    • HTML全文
    • 参考文献(8)
    • 相关文章
    • 施引文献
  • 资源附件(0)

/

返回文章
返回