镍对大鼠肝细胞内离子和线粒体含量的影响

Effect of nickel sulfate on ion concentration and content of mitochondrion in liver cells of rats

摘要:
目的探讨镍对大鼠肝细胞内Ca²⁺、Mg²⁺、Ni²⁺、Na⁺、H⁺和线粒体含量的影响。
方法健康Wistar大鼠腹腔注射硫酸镍1.25, 25, 5mg/kg染毒, 1次/d, 连续21d。染毒结束次日激光扫描共聚焦显微术测定肝细胞内离子浓度和线粒体含量。
结果染毒组动物肝细胞内Ca²⁺、Ni²⁺、Na⁺、H⁺均增加, Mg²⁺和线粒体含量下降。
结论 NiSO₄染毒可造成肝细胞内离子浓度和线粒体含量的改变。

Abstract:
Objective To study the toxic effect of nickel sulfate on the liver cells in rats.
Methods Nickel sulfate was injected intraperitoneally for Wistar rats daily at different doses(1.25, 2.50, 5.00 mg/kg)for 21 days.On the 22^nd day, to measure ion concentration of Ca²⁺, Mg²⁺, Ni²⁺, Na⁺ and H⁺ and the content of mitochondrion in the liver cells with alser confocal scanning microscopy.
Results In the liver cells, the levels of Mg²⁺and the content of mitochondrion were lower than those in the control rats, but the ion concentraton of Ca²⁺, Ni²⁺, Na⁺and H⁺ increased.
Conclusion The disorder of the ion concentration and the content of mitochondrion in the liver cells were caused by nickel sulfate and its hurt in the liver cells.