沥青烟对小鼠肺组织细胞周期的影响

Effect of bitumen fume on cell circle of lung cells in mice

摘要:
目的观察沥青烟对小鼠肺组织细胞周期的影响, 研究沥青致癌和致突变作用的机制。
方法用沥青烟对小鼠进行不同剂量和不同时间的染毒, 应用流式细胞仪进行小鼠肺组织细胞DNA含量的检测和细胞周期的分析。
结果随着沥青烟染毒时间和剂量的增加, 小鼠肺组织细胞G1期细胞数下降, S期阻滞, 进入G2/M期的细胞减少, 细胞增殖指数(PI)增加, 异倍体指数(DI)升高。
结论沥青烟可以对小鼠肺组织细胞周期产生影响, 主要表现在S期DNA合成增加, 细胞的增殖能力增强。

Abstract:
Objective To observe the effects of bitumen fume on cell circle of lungtissue cells in mice and to investigate the carcinogenic and mutagenic mechanism of bitumen fume.
Methods The experimental mice were forced to inhale the bitumen fume at different exposure level and different time periods.The cell circle was analyzed by flow cytometry.
Results The bitumen fume could result in the decrease of the cell number of G1 phase, the block of S phase, the delay of G2/M phase, the increase of cell proliferation index(PI)and the incr ease of DNA aneuploid index(DI).All the changes were correlated with the exposure level and the time of action.
Conclusion The bitumen fume could affect the cell circle of lung tissue cells and promote cell proliferation in mice.