双抗体夹心ELISA法检测葡萄球菌B型肠毒素

Enzyme-linked immunosorbent assay for detection of Staphylococcal Enterotoxin B

摘要:
目的建立一种敏感、快速的酶免疫测定方法, 用于定量检测人工污染食品中金黄色葡萄球菌肠毒素B(SEB)。
方法采用生物素-链亲素系统放大的双抗体夹心酶联免疫吸附方法, 以兔抗葡萄球菌肠毒素B(SEB)多克隆抗体作为包被抗体, 以生物素标记的抗SEB单克隆抗体2D1作为第二抗体进行测定。
结果在0.078~10.000μg/L的浓度范围内, SEB标准品浓度与吸光度值线性关系良好, 相关系数为r=0.9876, 平均变异系数为4.99%。
结论该方法灵敏度高、准确性好、稳定性强, 而且简便、快速, 适用于食品样品中SEB的定量测定。

Abstract:
Objective To establish a two-site antibo dyenzyme-linked immunosorbent assay(ELISA)for detecting food specimens artifically contaminated with employed as capture antibody.
Methods A monoclonal antibody to staphylococcus enterotox in B(SEB)2D₁ was used as indicator antibody.The sensitivity of ELISA was improved by biotin Ostrepra bindins system.
Results Calibration curves for SEB were linear from 0.078-10.000 μg/L(r=0.987 6), and the mean co-efficient of variation(CV)was 4.99%.
Conclusion This method is simple, rapid with high sensitivity and stablity, which may be suitable for quantitative measurement of the SEB in both food specimens.