锌铁调控蛋白4cDNA片断的获得及mRNA表达

Obtaining ZIP4 cDNA and expression of ZIP4 mRNA in mice

摘要:
目的获得锌铁调控蛋白4(ZRT, IRT-likeprotein 4, ZIP4)cDNA片断, 并了解ZIP4mRNA在小鼠各组织中的表达情况。
方法用反转录多聚酶链反应法(RT-PCR)获得ZIP4cDNA片断并比较ZIP4mRNA在各组织中的表达量。
结果 RT-PCR获得单一条带的片断, 其大小475bp, 并经测序确认; 在小肠、肝、睾丸等组织中检测到ZIP4mRNA表达, 其中小肠表达量最高, 心、脾、脑、肾、肺等组织中未见ZIP4mRNA表达。
结论获得ZIP4cDNA片段; ZIP4主要表达于小肠, 提示ZIP4可能在锌吸收中具有重要作用。

Abstract:
Objective To obtain the fragment of 2RT, IRT-like protein 4(ZIP4)cDNA and to comprehend the level of ZIP4 mRNA expression in various tissues.
Methods The fragment was obtained by RT-PCR and was sequenced.The expression of ZIP4 mRNA was also examined by RT-PCR and was compared with β-actin that served as the control.
Results A proper single fragment of 475bp was obtained with the sequence conformable to the corresponding fragment sequence of reported ZIP4 cDNA.The ZIP4 mRNA was detected in liver, testis and small intestine, in which the highest expression was observed.However, the expression of ZIP4 mRNA was not detected in heart, spleen, cerebrum, lung and kidney.
Conclusion The highest expression of ZIP4 mRNA in small intestine suggests a physiological functional importance of ZIP4 in zinc absorytion.