壬基酚对F1子代大鼠脑组织P450表达的影响

Effects of nonylphenol on brain cytochrome P450 gene expression of F1 generation rats

摘要:
目的利用芯片技术探讨壬基酚(nonylphenol, NP)对F1子代大鼠脑组织细胞色素P450基因表达的影响。
方法 NP染毒F0亲代母鼠, 分别提取NP组和对照组F1子代2日龄雄性大鼠的脑组织mRNA, 分别用cy5和cy3逆转录标记, 与Mouse40S基因表达谱芯片杂交, 并对cy3、cy5荧光信号做扫描分析。
结果芯片结果显示, 实验组已知功能差异表达基因有16条, 其中细胞色素P450基因家族的有3条, 2条上调, 1条下调。
结论 NP可影响大鼠脑组织细胞色素P450基因的转录水平。

Abstract:
Objective To explore the effects of nonylphenol on brain cytochrome P450 gene experession of F1 generation rats by means of microarry technique.
Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence.Subsequently, the cDNA probes were hybridized to the Mouse 40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed.
Results Sixteen identified genes expressed differently, including three cytochrome P450 genes, in which two up-regulated and one down-regulated.
Conclusion Nonylphenol can affect the expression of some cytochrome P450 genes of rat brain.