铅对小鼠海马c-jun表达及学习记忆功能影响

文涛; 孙黎光; 宗志宏; 高明奇; 刘素媛; 金心

铅对小鼠海马c-jun表达及学习记忆功能影响

Effect of lead exposure on capacity of learning and memory and expression of c-jun gene in mice

摘要

摘要:
目的观察铅对小鼠学习记忆功能及海马区cjun表达的影响。
方法 5~6周龄小白鼠交配后, 通过饮水饲以不同浓度醋酸铅2.4, 4.8, 9.6mmol/L。小鼠子代自胚胎期始即暴露于醋酸铅。幼鼠出生后, 先通过哺乳接触铅, 断乳后则自行饮用与母鼠饮用浓度相同的含铅水。6周后用跳台学习试验测试小鼠学习记忆能力; 最后处死小鼠, 用RTPCR法观察各组小鼠海马区c-jun mRNA的表达状况。
结果 3个染铅组小鼠跳台学习成绩显著低于对照组(P < 0.01);3个染铅组小鼠c-jun mRNA水平均明显降低, 并具有剂量效应关系。
结论 c-jun基因表达水平的下降可能是铅致小鼠学习记忆功能损害的分子机制之一。

Abstract:
Objective To observe the effect of the capacity of learning and memory and chronic lead contaminant on the expression of c-jun mRNA in hippocampus of mice.
Methods Chronic acetic lead contaminant was applied on mice(2.4, 4.8, 9.6 mmol/L)for 6 weeks.The step down test was used to measure the capacity of learning and memnory of each group.At the end of the experiment, RT-PCR was used to observe the expression of c-jun mRNA in hippocampus after the induction by step down test.
Results (1) In step down test the correct percentage of lead group significantly decreased compared with the control(P < 0.01).(2)Results of c-jun RT-PCR: The expression of c-jun was lowest in hippocampus of high lead group; was lower in middle lead group; was higher in low lead group.The expression of control group was highest.
Conclusion The decrease of c-jun gene expression level in hippocampus had a great link with the impairment of learning and memory induced by lead in mice, and this maight be one of the molecule mechanisms of lead-induced impairment of learning and memory.

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