B(a)P对鲫鱼全脑NO、NOS水平的影响

Effect of B(a)P on induction of NO and NOS in brain of carassius auratus

摘要:
目的探讨苯并(a)芘B(a)P对鲫鱼全脑一氧化氮(NO)和一氧化氮合酶(NOS)的影响及水生态毒理学机制, 同时为苯并(a)芘的水污染监测探寻灵敏简便的亚致死指标。
方法设立空白对照、溶剂对照、3×10^-4~3×10^-1mg/LB(a)P处理组共6个实验组, 分别于染毒后1, 3, 6和12d取鲫鱼全脑进行检测。
结果全脑一氧化氮(NO)、一氧化氮合酶(NOS)水平与苯并(a)芘存在着剂量及染毒时间依赖关系。
结论引起体内NOS活性升高及NO的过量产生是苯并(a)芘在鲫鱼体内发挥毒性的机制之一。

Abstract:
Objective To study the effect of B(a)Pon nitric oxide(NO)and nitric oxide synthase(NOS)activity in brain of carassius auratus, and to explore the toxic mechanism of B(a) Pin aquatic ecotoxicology.Meanwhile, to find sensitive and convenient sublethal biomarkers.
Methods Six groups were incorpor ated, including blank control, carrier control, and 3×10^-4-3×10^-1 mg/L B(a) P groups.After an exposure of 1, 3, 6 d and 12 d respectively, determining the biomarkers.
Results NO level and NOS activity had a dose-and time-dependent change after exposure to B(a) P.
Conclusion The induction of NOS and NO was one of the means by which B(a) Pdamaged carassius auratus.