八氯二丙醚对中国仓鼠肺细胞染色体畸变作用

Chromosome aberration in Chinese Hamster Lung Cells induced by Octachlorodipropyl Ether

摘要:
目的研究八氯二丙醚(S₂)对中国仓鼠肺细胞(CHL)染色体畸变作用。
方法测定S₂对CHL细胞的半数抑制浓度(IC₅₀), 根据IC₅₀设立不同剂量组, 进行正式试验, 分别观察24h, 48h及加S₉后的染色体畸变情况, 根据标准进行结果判定。
结果染毒24h为可疑阳性反应, 染毒48h以及加入活化系统染毒6h为阳性反应。
结论 S₂可引起CHL细胞染色体产生畸变作用。

Abstract:
Objective To explore the effects of octachlorodipropyl ether (S₂)on chromosome aberration in CHL Cells.
Methods To deter mine the IC₅₀ of S₂ on CHL cells, then to establish the scope of doses acco rding to the IC₅₀ and to do cells Chromosome aberration.According to the standard positive response the results were judged.
Results Marginal positive response was found at 24 h after the treatment with S₂.Positive responses were found at 48h after treatment or at 6 h after the addition of S-9 mixture.
Conclusion S₂ could induce Chromosome aberration in CHL cells.