高效氯氰菊酯对德国小蠊Na-K-ATP<sub>ase</sub>抑制效应

马义; 付荣恕; 霍新北; 马民

高效氯氰菊酯对德国小蠊Na-K-ATP_ase抑制效应

Inhibition of high effect cypermethrin on Na-K-ATP_ase of blattella germanica

摘要

摘要:
目的通过研究高效氯氰菊酯与德国小蠊Na-K-ATP_ase活性水平的量效与时效关系, 进一步完善高效氯氰菊酯作用机制的研究, 为德国小蠊的防制工作提供理论基础和方法参考。
方法参照国标GB13917.1~13917.8-92《农药登记卫生杀虫剂室内药效试验方法》喷施一定剂量不同浓度的高效氯氰菊酯, 用ATP酶测定试剂盒测定德国小蠊Na-K-ATP_ase活性。
结果 48h内高效氯氰菊酯对德国小蠊Na-K-ATP_ase活性的抑制作用呈浓度梯度效应, 高效氯氰菊酯的浓度与德国小蠊72h的死亡率和144h内对Na-K-ATP_ase活性的抑制时效呈正相关。
结论 Na-K-ATP_ase是高效氯氰菊酯的作用靶标之一, Na-K-ATP_ase敏感度降低而产生的靶标抗性可能是德国小蠊对高效氯氰菊酯产生抗药性的重要原因之一。

Abstract:
Objective To undestand the operational mechanisms of high effect cypermethrin and to provide theoretical basis and useful methods by researching the dosage effect and the time effect between high effect cypermethrin and Na-K-ATP_ase activities of Blattella germanica.
Methods Quantitative high effect cypermethr in of different concentration was sprayed according to the method of GB 13917.1-13917.8-92 for insecticide registered testing indoor effect.The activities of Na-K-ATP_ase were determined by using ATP_ase Kit.
Results Inhibition of high effect cypermethr in on Na-K-ATP_ase activities of Blattella germanica showed concentration gradient effect in 48 hours.The correlation of concentration of high effect cypermethrin and inhibiting time effect on Na-K-ATP_ase activities of Blattella germanica in 144 hours and death rate of Blattella germanica in 72 hours was positive relationship.
Conclusion Na-K-ATP_ase is one of the targets of high effect cypermethrin.Because of sensitivity decrease of Na-K-ATP_ase, tar get resistance may be one of the important resistant mechanisms to high effect cypermethrin.

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高效氯氰菊酯对德国小蠊Na-K-ATPase抑制效应

Inhibition of high effect cypermethrin on Na-K-ATPase of blattella germanica

高效氯氰菊酯对德国小蠊Na-K-ATP_ase抑制效应

Inhibition of high effect cypermethrin on Na-K-ATP_ase of blattella germanica