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茅海燕, 卢亦愚, 汤宏峰, 严菊英, 李婵, 谢荣辉, 冯燕. 半巢式RT-PCR法检测呼吸道合胞病毒[J]. 中国公共卫生, 2004, 20(12): 1448-1450.
引用本文: 茅海燕, 卢亦愚, 汤宏峰, 严菊英, 李婵, 谢荣辉, 冯燕. 半巢式RT-PCR法检测呼吸道合胞病毒[J]. 中国公共卫生, 2004, 20(12): 1448-1450.
shu
MAO Hai-yan, LU Yi-yu, TANG Hong-feng, . Detection of respiratory syncytial virus by semi-nested RT-PCR[J]. Chinese Journal of Public Health, 2004, 20(12): 1448-1450.
Citation: MAO Hai-yan, LU Yi-yu, TANG Hong-feng, . Detection of respiratory syncytial virus by semi-nested RT-PCR[J]. Chinese Journal of Public Health, 2004, 20(12): 1448-1450.
shu

半巢式RT-PCR法检测呼吸道合胞病毒

Detection of respiratory syncytial virus by semi-nested RT-PCR

    摘要
  • 摘要:
      目的   建立快速、敏感、特异的人呼吸道合胞病毒(Respiratory syncytialvirus, RSV)检测方法。
      方法   根据RSVF基因的保守序列设计3条引物, 建立半巢式RT-PCR(semi-nested RT-PCR)检测方法.用该方法检测125例患急性下呼吸道感染的婴幼儿的鼻咽吸引物(nasophary ngealaspirates, NPA)标本, 同时对标本进行病毒分离和直接免疫荧光法检测。
      结果   该半巢式RT-PCR灵敏度为0.1TCID50, 用该方法在125例标本中共检出RSV阳性标本63例, 阳性率50.4%。
      结论   建立快速、敏感、特异的RSV半巢式RT-PCR检测方法, 该方法适用于临床早期诊断和流行病学监测。

     

    Abstract:
      Objective   To develop a rapid, sensitive, specific method to detect human respiratory syncytial virus(RSV).
      Methods   Three pieces of primer were designed from the conservative F gene of RSV and a semi-nested RT-PCR method was achieved.This method was used to diagnose 125 nasopharyngeal aspirates obtained from children with lower respiratory tract infection, at the same time the specimens were also analyzed by viral isolation and direct fluorescent-antibody assay(DFA).
      Results   The sensitivity of the semi-nested RT-PCR assay was determined to be 0.1 TCID50; 63 cases(50.4%) were identified RSV-positive by the semi-nested RT-PCR.
      Conclusion   The semi-nested RT-PCR was a rapid, sensitive, specific method for diagnosis of RSV infection, and it could be used for early clinical diagnosis as well as epidemiological surveillance.

     

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