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石玉玲, 李林海, 陈清, 楚心唯, 王云龙, 马清钧, 曹诚, 俞守义. 不同地区人群SARS血清流行病学调查[J]. 中国公共卫生, 2004, 20(12): 1476-1477.
引用本文: 石玉玲, 李林海, 陈清, 楚心唯, 王云龙, 马清钧, 曹诚, 俞守义. 不同地区人群SARS血清流行病学调查[J]. 中国公共卫生, 2004, 20(12): 1476-1477.
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SHI Yu-ling, LI Lin-hai, CHEN Qing, . Seroepidemiological study On SARS-CoV IgG antibody of different populations from several areas[J]. Chinese Journal of Public Health, 2004, 20(12): 1476-1477.
Citation: SHI Yu-ling, LI Lin-hai, CHEN Qing, . Seroepidemiological study On SARS-CoV IgG antibody of different populations from several areas[J]. Chinese Journal of Public Health, 2004, 20(12): 1476-1477.
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不同地区人群SARS血清流行病学调查

Seroepidemiological study On SARS-CoV IgG antibody of different populations from several areas

    摘要
  • 摘要:
      目的   分析严重急性呼吸综合征(Severe Acute Respiratory Syndrome, SARS)流行期间不同地区人群及SARS临床诊断病例血清特异性抗体(SARS-CoV IgG)变化规律以及健康人群是否存在隐性感染。
      方法   采用酶联免疫方法(ELISA)及间接免疫荧光法(IFA), 对香港、澳门、北京、广州地区1453名健康人及广州和北京地区257例SARS临床诊断病例进行血清SARS-CoVIgG抗体检测, 并跟踪检测患者病后不同时间(发病3~270d)血清抗体变化情况。
      结果   4个地区健康人群(包括160名密切接触者)中, 用ELISA法检出2例SARS-CoVIgG抗体阳性, 阳性者进一步用IFA检测则为阴性; 广州地区200名健康体检者同时采用ELISA和IFA检测, 结果均为阴性。ELISA和IFA同时检测257例临床诊断病例发病20d后的血清SARS-CoVIgG抗体, 2种方法检出的阳性率均为90%。ELISA法跟踪检测257例临床诊断病例发病后3~270d血清IgG抗体, 抗体滴度在发病后4~6个月内随时间延长逐渐升高, 多数病人抗体在第4~6个月达到高峰, 并持续到第8个月, 部分病人抗体达高峰后开始下降, 但至第9个月仍阳性.
      结论   健康人群中隐性感染率极低, 血清SARS-CoVIgG抗体在病程后期有诊断价值, 血清特异性抗体可持续9个月以上。

     

    Abstract:
      Objective   To analyze dynamic rules of SARS coronavirus(SARS-CoV)IgG antibody of probable patients with severe acute respiratory syndrome(SARS)during the epidemic period in 2003 and whether the asymtomatic infections existing in healthy population from several areas.
      Methods   Indirect immunofluo rescent assay(IFA)and double-antigen sandwich enzyme-linked immunosorbent assay(ELISA)were used to detect the SA RS coronavirus-specific IgG antibodies in serum specimens, including 1453 healthy persons from Hongkong, Marco, Guangzhou and Peking and 257 SARS probable patients from Guangzhou and Peking.The dynamics of the serum antibodies of SARS patients were observed from 3 to 270 days after onset of symptoms.
      Results   Among healthy persons including 160 close contacters of SARS patient, only 2 were positive by the sandwich EL ISA.These two specimens were negative by IFA.90% of 257 patient serum specimens after 20 days of disease onset showed positive SARS-CoV IgG either using ELISA or IFA.Of 257 SARS patients, antibody titers increased steadily in early 4 to 6 months after onset of SARS.The titers of most cases came to the peak in the 6th month and maintained the titers for two months.Then antibody titers declined in some cases.How ever, all specimens still were positive for SARS-CoV IgG in the 9th month of the disease onset.
      Conclusion   Few asymtomatic infectious patients may exist during SARS epidemic.Serum IgG antibody has diagnostic value for SARS in the late course of the disease and the antibodies present more than 9 months.

     

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