维生素E琥珀酸酯诱导人胃癌细胞分化的作用

Vitamin E succinate induced differentiation in human gastric carcinoma cell line SGC-7901

摘要:
目的研究维生素E琥珀酸酯(VES)对胃癌细胞的诱导分化作用。
方法 5, 10μg/ml维生素E琥珀酸酯处理人胃癌SGE-7901细胞, 分别采用噻唑蓝(MTT)法检测细胞的生长; ³H-TdR掺入法测定DNA合成速率; 流式细胞术检测细胞周期的分布; 分光光度法检测分化标志酶的活性。
结果 VES可使细胞形态学改变; 抑制SGC-7901细胞的生长, 降低DNA的合成速率; 使细胞出现G0/G1期停滞; 分化标志酶碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)活性降低。
结论 VES可诱导SGC-7901细胞分化并抑制其恶性生长。

Abstract:
Objective To study differentiation-induction effect of vitamin E succinate(VES)on human gastric carcinoma cell line SGC-7901.
Methods At the base of cell culture in vitro, the effect of differentiation-inducing activity of VES at the dose of 5, 10 μg/ml were measured by MTT method for cell growth rate; by ³H-TdR incorporation method for DNA synthetic speed; by flow cytometry for cell cycle distribution, by spectrophotometer for enzyme activity.
Results After treated with VES, SGC-7901 cells were led to morphologic change, growth inhibition, DNA synthetic speed decrease, cell cycle arrest, and ALP, LDH activity suppressant.
Conclusion VES can induce SGC-7901 human gastric cancer cells to undergo differentiation.