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许能锋, 陈锦辉, 冯羯羢, 陈菁. 大肠埃希菌O157:H7在8种食品中存活力观察[J]. 中国公共卫生, 2004, 20(11): 1324-1325.
引用本文: 许能锋, 陈锦辉, 冯羯羢, 陈菁. 大肠埃希菌O157:H7在8种食品中存活力观察[J]. 中国公共卫生, 2004, 20(11): 1324-1325.
XU Neng-feng, CHEN Jin-hui, FENG Jie-rong, . Investigation on survival of escherichia coli O157: H7 in eight kinds of food[J]. Chinese Journal of Public Health, 2004, 20(11): 1324-1325.
Citation: XU Neng-feng, CHEN Jin-hui, FENG Jie-rong, . Investigation on survival of escherichia coli O157: H7 in eight kinds of food[J]. Chinese Journal of Public Health, 2004, 20(11): 1324-1325.

大肠埃希菌O157:H7在8种食品中存活力观察

Investigation on survival of escherichia coli O157: H7 in eight kinds of food

  • 摘要:
      目的   探讨肠出血性大肠埃希菌(EHEC)O157: H7在不同食品中的存活力。
      方法   采用EHECO157: H7933第5代菌株人工污染8种食品, 染菌量为7log10cfu/ml·g; 对该菌在4、25℃条件下的存活力进行实验观察。
      结果   EHECO157: H7在全奶、奶粉、鱼肉香肠及猪肉松4种动物性食品中存活较长时间(70d以上), 且存活菌量呈先升后降的变化趋势, 在2种温度条件下均能增殖1~2log10cfu/(ml·g), 到达高峰后呈对数衰减趋势, 42d后仍有3~6log10cfu/ml·g活菌量; 在橙汁汽水和乳酸菌饮料中存活时间相对较短, 分别为2~5d和10~27d, 4℃橙汁汽水和25℃乳酸菌饮料在衰减至4log10cfu/(ml·g)后急剧下降: 在草莓酱和苹果酱中, 两种温度条件下EHECO157: H7均呈间断性检出。
      结论   EHECO157: H7在常见食品中的存活能力强。加强食品特别是动物性食品及饮料的卫生管理是预防和控制该菌感染的重要措施。

     

    Abstract:
      Objective   To investigate the survival of Escherichia coli O157: H7 in eight kinds of food.
      Methods   Eight kinds of food were collected and contaminated by the bacterial suspension of EHEC O157: H7 933 strain, the count of contaminated bacteria was 7 log 10 cfu/ml·g. Then the survival of E.coli O157: H7 was observed experimentally at certain temperature(4, 25℃).
      Results   E.coli O157: H7 lived longer(> 70 days)in animal food than other food. The trend of bacterial survival number rose firstly and then dropped. The bacteria both proliferated 1-2 log 10 cfu/ml·g at two kinds of temperature and decreased logarithmically after reaching the peak. The bacterial count was still 3-6 log 10 cfu/ml·g after 42 days. The bacteria lived comparatively short(2-5 days in orange juice, 10-27 days in lactic drink).The bacterial count rapidly decreased to 0 when it cut down on 4 log 10 cfu/ml·g.E. coli O157: H7 was found intermittently in both the strawberry jam and the apple jam.
      Conclusion   E.coli O157: H7 had strong survival in ordinary food. The major measure to prevent and control its infection was to strengthen the sanitary administration of food, especially animal food, and beverage.

     

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