亚砷酸钠对淋巴细胞毒性及抗氧化物拮抗作用
Cytotoxicity of arsenic to lymphocytes of rats and antagonism of antioxidant
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摘要:目的 研究不同浓度的亚砷酸钠对体外培养的大鼠淋巴细胞活力的影响及亚硒酸钠和抗坏血酸的拮抗作用。方法 体外分离大鼠外周血淋巴细胞后, 施加处理因素, 在37℃条件下恒温培养, 用水溶性染料(AlamarBlue)摄入法定量检测淋巴细胞活力。结果 亚砷酸钠浓度大于5μmol/L时, AlamarBlue的还原率显著低于对照组, 且呈剂量反应关系; 用5μmol/L的亚硒酸钠预处理细胞24h后, 10μmol/L亚砷酸钠组AlamarBlue的还原率显著增高, 接近空白对照组还原率; 而用50μmol/L的抗坏血酸预处理后, 还原率有所增高, 但未达到对照组水平。结论 亚砷酸钠可抑制淋巴细胞活力, 抗氧化物可一定程度的拮抗三价砷的细胞毒性作用。Abstract:Objective To investigate the effect of different concentrations of sodium arsenite on the survival of the lymphocytes of rats cultured in vitro and the antagonism of sodium selenlite and ascorbic acid.Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with agents under 37, the survival of lymphocytes was determined by the percents of reduced AlamarBlue.Results The rates of reduced AlamarBlue in sodium arsenite above 5μmol/L were significantly higher than the controls and with dose-response relation. After pretreatment of sodium selenite and ascorbic acid for 24 hours, the rates of reduced A lamarBlue in sodiumarsenite of 10μmol/L increased accordingly.Conclusion Sodium arsenite can inhibit the survival of lymphocytes of rats and the antioxidants can antagonize the cytotoxicity of arsenic.
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