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袭著革, 晁福寰, 孙咏梅, 李官贤, 刘中文, 张华山, 杨丹凤, 张伟. 室内生源性多环芳烃对DNA的氧化损伤[J]. 中国公共卫生, 2004, 20(9): 1034-1036.
引用本文: 袭著革, 晁福寰, 孙咏梅, 李官贤, 刘中文, 张华山, 杨丹凤, 张伟. 室内生源性多环芳烃对DNA的氧化损伤[J]. 中国公共卫生, 2004, 20(9): 1034-1036.
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XI Zhu-ge, CHAO Fu-huan, SUN Yong-mei, . Study on oxidative DNA damage induced by PAHs from household pollution[J]. Chinese Journal of Public Health, 2004, 20(9): 1034-1036.
Citation: XI Zhu-ge, CHAO Fu-huan, SUN Yong-mei, . Study on oxidative DNA damage induced by PAHs from household pollution[J]. Chinese Journal of Public Health, 2004, 20(9): 1034-1036.
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室内生源性多环芳烃对DNA的氧化损伤

Study on oxidative DNA damage induced by PAHs from household pollution

    摘要
  • 摘要:
      目的   了解室内生活污染来源-吸烟和烹调产生的多环芳烃(PAHs)种类与含量, 及其对DNA的氧化损伤作用。
      方法   采集室内烹调油烟和环境烟草烟雾颗粒物, 应用气相色谱-质谱-选择性离子监测技术(GC-MS-SIM)定量检测10种PAHs, 并用10种混合PAHs经气管灌注染毒大鼠, 用液相色谱结合电化学检测技术测定肺组织DNA中产生的氧化损伤标志物8-羟基脱氧鸟苷(8-OHdG)。
      结果   PAHs广泛存在于烹调油烟冷凝物、油烟颗粒物以及环境烟草烟雾的主、侧流烟雾颗粒物之中。其标准混合物可诱导大鼠肺组织DNA氧化损伤形成8-OHdG, 并呈现明确的剂量-反应关系。
      结论   PAHs的遗传毒性效应和潜在的致癌效应存在着一条氧化代谢产生羟基自由基进攻核酸的途径。

     

    Abstract:
      Objective   To study the kinds and concentration of PAHs released from household pollution-environmental tobacco smoke and heated oil smoke, and the effect of inducing oxidative DNA damage.
      Methods   Collected particles of heated oil smoke and environmental tobacco smoke were quantitatively detected by GC-MS-SIM.With the trachea instillation, 8-hydroxy-2'-deoxyguanosine, the biomarker of oxidative DNA damage from the rat lung tissue induced by the 10 kinds of PAHs', was detected by HPL C-EC.
      Results   PAHs widely existed in the condensed matters and the particle matters such as heated oil, the main and the side stream tobacco smoke.The mixture compounds of 10 kinds standard PAHs can induce oxidative DNA damage of the rat lung tissue to form 8-OHdG, and had significnat dose-response relationship.
      Conclusion   Genotoxic and carcinogenic effects of PAHs had a path of after metabolized to yield hydroxyl radicals(°OH)to attack DNA.

     

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