镉染毒大鼠肾损害与钙稳态关系的研究

Study on relation between renal damage and calcium homeostasis in cadmium-poisoned wistar rats

摘要:
目的探讨镉中毒性肾损害的细胞分子学机制。
方法通过腹腔注射Cd²⁺14mg/(kg°bw)制备肾损伤模型, 观察镉性肾损害时胞浆中钙含量的变化及其与肾损害之间的关系。
结果随着隔作用时间的延长, 肾皮质中尿蛋白、尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)不断升高, 肾小球滤过率(GFR)减少, 肾胞浆中钙显著增高, Ca²⁺-Mg²⁺-ATPase活性也随之升高。
结论镉性肾损伤中, 细胞钙稳态受到破坏, 从而导致了肾功能的损害。

Abstract:
Objective To study the cell molecular mechanism of cadmium-induced renal damage.
Methods With Cadmium dose 1.4 mg/kg Cd²⁺ by celiac injection to make renal damage model in order to observe relation between renal damage and changes of intracellular calcium content.
Results The content of protein, N-acetyl-β-glucosaminidase in urine increased continually, GFR decreased, the intracellular calcium increased relatively.
Conclusion The damage of intracellular calcium homeostasis leaded to the renal function disorder.