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田玉慧, 李万里, 任金平, 赵玉芝, 韩金利. 大豆异黄酮对去卵巢大鼠骨形成蛋白的影响[J]. 中国公共卫生, 2004, 20(9): 1079-1080.
引用本文: 田玉慧, 李万里, 任金平, 赵玉芝, 韩金利. 大豆异黄酮对去卵巢大鼠骨形成蛋白的影响[J]. 中国公共卫生, 2004, 20(9): 1079-1080.
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TIAN Yu-hui, LI Wan-li, REN Jin-ping, . Effect of soybean isoflavone on expression of bone BMP2 and TGF-β1 in ovariectomized rats[J]. Chinese Journal of Public Health, 2004, 20(9): 1079-1080.
Citation: TIAN Yu-hui, LI Wan-li, REN Jin-ping, . Effect of soybean isoflavone on expression of bone BMP2 and TGF-β1 in ovariectomized rats[J]. Chinese Journal of Public Health, 2004, 20(9): 1079-1080.
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大豆异黄酮对去卵巢大鼠骨形成蛋白的影响

Effect of soybean isoflavone on expression of bone BMP2 and TGF-β1 in ovariectomized rats

    摘要
  • 摘要:
      目的   探讨大豆异黄酮和钙对去卵巢大鼠无机盐、骨形成蛋白2(BMP2)和转移生长因子-β1(TGF-β1)的影响。
      方法   取28只雌性SD大鼠随机分为4组: 假手术组、去卵巢组、去卵巢补钙组〔50mg/(kg°d)〕和去卵巢加大豆异黄酮组〔大豆异黄酮100mg/(kg°d)〕, 10周后测骨密度、血清和尿液钙、钠、钾含量。用免疫组化法测骨骼中BMP2和TGF-β1的表达。
      结果   去卵巢组大鼠血清和尿钙、钠含量显著增高, 而骨密度、血清和尿钾及骨BMP2和TGF-β1表达降低; 去卵巢加大豆异黄酮后大鼠血清钙和尿钙、钠显著降低, 骨密度和骨BMP2、TGF-β1显著性升高(P < 0.05或P < 0.01)。单纯补钙组与去卵巢组相比只有TGF-β1有显著性差异(P < 0.05)。
      结论   大豆异黄酮可调节去卵巢大鼠无机盐代谢, 促进BMP2和TGF-β1表达, 预防去卵巢大鼠的骨质疏松。

     

    Abstract:
      Objective   To study the effect of soybean isoflavone and calcium on mineral and the expression of bone BMP2, TGF-β1 in ovariectomized rats.
      Methods   Twenty eight female SD rats were randomly divided into four groups: sham, ovariectomized, ovariectomized added calcium〔50 mg/(kg°d)〕, ovariectomized added soybean isoflavone〔100 mg/(kg°d)〕.The content of serum and urine calcium, sodium, potassium were deter mined after ten weeks.The Streptavidin-Peroxidase HistostainTM-Plus Kits was used to detect bone BMP2 and TGF-β1 expression.
      Results   The calcium, sodium of serum and urine in ovariectomized group increased significantly.The bone mineral density, and potassium of serum and urine, bone BMP2 and TGF-β1 decreased significantly.The serum calcium and urine calcium, sodium in ovariectomized added soybean isoflavone group decreased significantly, but the bone mineral density and bone BMP2 and TGF-β1 expression increased significantly(P < 0.05 orP < 0.01).Only the TGF-β1 expression in ovariectomized added calcium group had significantly increace than that of ovariectomized group(P < 0.05).
      Conclusion   The soybean isoflavone may adjust the mineral metabolism and prompt the bone BMP2, TGF-β1 expression to prevent the osteoporosis of ovariectomized rats.

     

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