D-青霉胺和二巯基丙磺酸钠拮抗汞肾毒性

尹忠伟; 徐兆发; 于佳明; 杨敬华; 孙炜; 李晶

D-青霉胺和二巯基丙磺酸钠拮抗汞肾毒性

Antagonistic effects of D-penicillamine and sodium 2, 3-dimercato-1-propanesulfonate on nephrotxicity caused by mercury

摘要

摘要:
目的 D-青霉胺(DPA)和二巯基丙磺酸钠(DMPS)对汞肾毒性的保护作用。
方法 32只Wistar大鼠随机分成4组。第1, 2组分别皮下注射0.9%氯化钠和2.5mg/kgHgCl₂溶液。第3, 4组大鼠分别腹腔注射200μmol/kgDMPS和DPA, 2h后再皮下注射2.5mg/kgHgCl₂溶液。染毒12h后, 收集12h尿样, 测定尿N-乙酰-β-D-氨基葡萄糖苷酶(NAG)和尿碱性磷酸酶(ALP)活性、尿蛋白和尿汞含量。染毒48h后, 腹主动脉采血和切取肾皮质和肝脏, 分别测定血清尿素氮(BUN)含量和肾脏、肝脏中的丙二醛(MDA)和谷胱甘肽(GSH)含量、谷胱甘肽过氧化物酶(GSH-Px)活性、肝脏汞和肾脏汞含量。
结果 DMPS和DPA显著降低染汞大鼠组尿NAG、ALP活性, 血清尿素氮(BUN)和尿蛋白含量, 以及肾MDA和GSH含量及GSH-Px的活性, 但对尿汞肾汞含量影响不大。
结论预先注射的DMPS和D队能明显减轻急性汞致肾的损伤, DMPS的效果更强。

Abstract:
Objective To study the protective effects of d-penicillamine and sodium 2, 3-dimercato-1-propanesulfonate on attenuating nephrotoxicity towards rats exposed to mercuric chloride(Hg).
Methods 32 Wistar rats were divided into 4 groups.The rats in the first and second groups were respectively subcutaneously(sc.)injected with 0.9% saline and 2.5 mg/kg mercuric chloride, the rats in the third and fourth groups were pretreated with DPA and DMPS respectively at the dose of 200μmol/kg and after 2 h the rats were sc.injected with 2.5 mg/kg Hg.24-hour urine samples were determined for NAG, ALP activities and urinary protein levels.After 48-hour administration the BUN, MDA, GSH and GSH-Px levels were determined.
Results DMPS and DPA reduced significantly urinary NAG, ALP activities, urinary proteins and BUN contents, and Hg contents in the kidneys and livers, however there was not any influence on urinary and renal Hg contents.
Conclusion DMPS attenuated the damage of Hg in the kidney more efficiently than DPA.

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