2型登革病毒基因部分序列测定及氨基酸分析

舒莉萍; 左丽; 赵星; 李永念; 陈阿英

2型登革病毒基因部分序列测定及氨基酸分析

Genomic sequence determination and amino acid sequence deduction of dengue virus 2 type Mawei strain

摘要

摘要:
目的对登革病毒(DEN)-2贵州分离株的NS1、E基因部分序列进行测定并作氨基酸序列的预测分析。
方法碘化钠-异硫氰酸胍-氯仿法提取RNA, 用DENNS1基因区通用引物和DEN-2E基因区特异性引物经逆转录-聚合酶链反应(RT-PCR)扩增登革病毒核酸, 用纯化PCR产物测序后, 用DNASIS软件进行氨基酸序列的预测。
结果 DEN-2贵州分离株部分基因测序及氨基酸序列预测结果与DEN-2NGC株比较, 分离株的NS1基因区有8个碱基发生点突变和2个位点氨基酸改变, E基因区有1个碱基的插入和1个位点氨基酸改变。
结论 DEN-2贵州分离株和NGC株NS1、E基因区基因序列存在差异。

Abstract:
Objective To analyze the partial gene sequence in NS1, E gene of dengue virus type 2 Mawei strain, and to deduce the amino acid sequence.
Methods Dengue virus type 2 Mawei strain and N GC strain were proliferated with C6/36 cells, respectively.The total viral RNA were extracted.The viral NS1 and E gene fragment were amplified with reverse transcriptase polymerase chain reaction(RT-PCR).Then the product of RT-PCR were sequenced, and the amino acid sequence was deduced by DNASIS software.
Results The E and NS1 gene of dengue virus type 2 Mawei strain were sequenced.Seven point mutations of NS1 gene and one base insertion of E gene were found, compared with DEN-2 NGC strain.This different sequence were accepted by GenBank.The accession number of NS1, E gene were A Y277402, A Y278226, respectively.
Conclusion The viral NS1 and E gene sequence were different between dengue virus type 2 Mawei strain and N GC strain.

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