SARS冠状病毒S基因序列及细胞抗原表位预测

Comparison of S genome sequence of SARS-CoV and prediction of B cell epitopes

摘要:
目的比较SARS冠状病毒各分离株S基因序列及氨基酸序列之间的差异, 预测SARS-CoV的S蛋白的抗原表位。
方法利用Lasergene软件包中的EditSeq从21株SARS冠状病毒分离株截取S基因序列并翻译成氨基酸序列, 然后用ClustalX软件对截取序列进行比较分析, 确定基准株, 最后用Protean软件对基准株进行抗原表位预测。
结果在21株分离株的S基因中有8株核苷酸序列、13株氨基酸序列没有发生点突变; 预测出78个可能性抗原表位。
结论 SARS-CoV的S蛋白相当保守, 只发生个别点突变, 可能性抗原表位多, 且在其中的14个区域中可能性最显著。

Abstract:
Objective To compare the differences of S genome and amino acids sequence f SARS-CoV isolates, analyze possible B cell epitopes of S protein.
Methods S genome sequences of SARS-CoV isolates are cut with Editseq program of Lasergene and then translated into amino acids sequences.To compare the difference of genome and amino acids sequences with Clustal X and then predict B cell epitopes with Protean program.
Results There are not point mutations in 8 genome sequences and 13 amino acids sequences of the S gene of 21 isolates and 78 of B cell epitopes in S protein are predicted.
Conclusion S genome sequences of SARS-CoV are rather conservative and only change a little.There are many possible anti2 genic epitopes in S protein, especially in 14 zones.