白细胞介素真核表达质粒的卡介苗佐剂作用

张俊才; 骆雪霞; 钟志宏; 马正海

白细胞介素真核表达质粒的卡介苗佐剂作用

Study and adjuvant effects of eukaryotic express plasmid of murine IL-2 and IL-3 to immunity of BCG

摘要

摘要:
目的研究白细胞介素-2(IL-2)和白细胞介素-3(IL-3)真核表达质粒的佐剂活性, 从而达到增强卡介苗(BCG)免疫效果的作用。
方法在结核分枝杆菌敏感动物豚鼠体内表达IL-2和IL-3真核表达质粒, 分别从细胞免疫和体液免疫水平以及组织水平上比较其对卡介苗免疫效果的调节作用和免疫器官的病理变化, 采取间接ELISA和MTT比色法检测及组织病理学检测。
结果间接ELISA检测显示, IL-2和IL-3真核表达质粒组抗体水平均高于BCG和空白对照组(P < 0.05);MTT比色法检测显示IL-2和IL-3真核表达质粒组细胞免疫水平均高于对照组(P < 0.01);组织病理学检测结果显示, IL-2和IL-3真核表达质粒组免疫器官淋巴滤泡增生均显著高于对照组。
结论 IL-2和IL-3真核表达质粒均能有效地促进机体体液和细胞免疫应答, 尤其是细胞免疫应答。

Abstract:
Objective Eukaryotic express plasmid of Murine IL-2(pCDNA₃IL-2)and eukaryotic express plasmid of Murine IL-3(pCDNA³IL-3)were used as adjuvants in the study.The experiments were conducted to compare the immune modulation effects and histological lesions of the two adjuvatns to mycobacterium bovis BCG vaccine according to the levels of cellular immunity, humoral immunity and histopathology in immune organs.
Methods The tested animals were guinea pigs.Fifty days after twice BCG vaccination samples were detected by indirect ELISA, MTT and histopathological lesions of immune organs.
Results pcDNA₃IL-2 and pcDNA₃IL-3(pcDNA₃IL-3 can both effectively enhance the humoral immunity and the cellular immunity of guinea pit induced by BCG(P < 0.05 or P < 0.01)and hyperplasia of histopathological lesions of immune organs.
Conclusion Eukaryotic Express Plasmid of Murine IL-2 and IL-3 can both promote the humoral immunity and the cellular immunity of guinea pig induced by BCG(P < 0.05 or P < 0.01), so they can be as a kind of potential adjuvants.

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