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刘珊, 孙贵范, 宛超, 孙鲜策, 王毅, 曲龙. 维生素C对香烟烟雾的氧化应激作用的影响[J]. 中国公共卫生, 2004, 20(8): 938-940.
引用本文: 刘珊, 孙贵范, 宛超, 孙鲜策, 王毅, 曲龙. 维生素C对香烟烟雾的氧化应激作用的影响[J]. 中国公共卫生, 2004, 20(8): 938-940.
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LIU Shan, SUN Gui-fan, WAN Chao, . Influence of vitamin C on oxidative stress induced by cigarette smoke solution in rat lymphoeytes[J]. Chinese Journal of Public Health, 2004, 20(8): 938-940.
Citation: LIU Shan, SUN Gui-fan, WAN Chao, . Influence of vitamin C on oxidative stress induced by cigarette smoke solution in rat lymphoeytes[J]. Chinese Journal of Public Health, 2004, 20(8): 938-940.
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维生素C对香烟烟雾的氧化应激作用的影响

Influence of vitamin C on oxidative stress induced by cigarette smoke solution in rat lymphoeytes

    摘要
  • 摘要:
      目的   了解维生素C(VC)对香烟烟雾溶液(CSS)作用下的大鼠淋巴细胞产生氧化应激的影响。
      方法   将香烟烟雾溶液作用于经VC预处理的大鼠淋巴细胞, 用2', 7'-二乙酰二氯荧光素(DCFH-DA)检测细胞内活性氧自由基(ROS)水平, 用彗星实验评价DNA的损伤状况, 同时检测细胞内超氧化物歧化酶(SOD)活性和脂质过氧化物(LPO)水平。
      结果   8×10-3支/mlCSS作用下大鼠淋巴细胞内ROS和LPO水平增高, DNA损伤增强, SOD活性降低。用50μmol/LVC干预后, 与相应非干预组相比细胞内ROS和LPO水平降低, DNA损伤减轻, SOD活性增强, 且均有显著性差异。
      结论   50μmol/LVC能保护大鼠淋巴细胞, 减轻CSS导致的氧化应激。

     

    Abstract:
      Objective   To learn how vitamin C(VC)influences oxidative stress of rat lymphocytes induced by cigarette smoke solution(CSS).
      Methods   8×10-3 cig/ml CSS was added to rat lymphocytes pretreated with or without VC, then intracellular reactive oxygen species(ROS)was detected by using 2', 7'-dichlorofluorescein diacetate(DCFH-DA), DNA damage was evaluated by comet assay, and lipid peroxide(LPO)and superoxide dismutase(SOD)activity were measured as well.
      Results   In contrast to CSS-treated cells pre-incubated with 50μmol/L VC, ROS, L PO level and DNA damage increased, and SOD activity decreased significantly in lymphocytes treated with CSS only.
      Conclusion   In rat lymphocytes, oxidative stress caused by CSS could be prevented by 50μmol/L VC effectively.

     

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