硒与维生素E对荷瘤小鼠组织的抗氧化作用

徐跃飞; 任凤; 隋静

硒与维生素E对荷瘤小鼠组织的抗氧化作用

Study on effect of Se and vitamin E on antioxidant action in tissues of mice bearing tumor cells

摘要

摘要:
目的观察硒(Se)与维生素2(VE)对H₂₂肝癌小鼠生长的抑制作用和对淋巴样器官SOD/LPO比值的影响。
方法选用40只健康昆明种小鼠, 随机分为生理盐水对照组、Se组、VE组及Se+VE组, 荷瘤后第2d分别注射一定剂量的受试物, 连续6d。采集脾和淋巴结, 测定其匀浆中过氧化脂质(LPO)含量和超氧物岐化酶(SOD)活性。
结果 1mg/(kg^°bw)Na₂SeO₃与5mg/(kg^°bw)VE能显著增加荷瘤小鼠淋巴样器官SOD/LPO比值(P < 0.01), 并抑制癌细胞的增殖, 0.2mg/(kg^°bw)Na₂SeO₃, 1mg/(kg^°bw)VE联合应用时效果更为显著。
结论 Se与VE在淋巴样器官中的抗氧化作用可能与维持机体免疫功能有关。

Abstract:
Objective To ivnestigate the effects of Se and vitamin E(VE)on the SOD/LPO values in the lymphoid organs of mices bearing tumor cells and on the proliferation of tumor cells.
Methods Forty healthy mices were chosen and randomly divided into control group, VE group, Se group and VE+Se group.After all mices were injected by abdominal cavity with tumor cells, experimental substances were used to replenish to the proper mices for 6 days.Spleen and lymph node were collected and used in determining contents of lipid perodide(LPO)and activities of superoxide dismutase(SOD).
Results Se of 1 mg/(kg^°bw)and VE of 5 mg/(kg^°bw)not only inhabited the proliferation of tumor cells, but also increasd SOD/LPO values in lymphoid organs of hosts bearing tumor cells, and the effect was more obvious when combination use of Se of 0.2 mg/(kg^°bw)with VE of 1 mg/(kg^°bw).
Conclusion The antioxidative role in the lymphoid organs of Se and VE were closely related to the immune function of the hosts.

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