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王春花, 刘克明, 张明月, 刘玉清. SOD基因表达与衰老的相关性[J]. 中国公共卫生, 2004, 20(8): 953-954.
引用本文: 王春花, 刘克明, 张明月, 刘玉清. SOD基因表达与衰老的相关性[J]. 中国公共卫生, 2004, 20(8): 953-954.
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WANG Chun-hua, LIU Ke-ming, ZHANG Ming-yue, . Correlation of SOD gene expression to senility[J]. Chinese Journal of Public Health, 2004, 20(8): 953-954.
Citation: WANG Chun-hua, LIU Ke-ming, ZHANG Ming-yue, . Correlation of SOD gene expression to senility[J]. Chinese Journal of Public Health, 2004, 20(8): 953-954.
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SOD基因表达与衰老的相关性

Correlation of SOD gene expression to senility

    摘要
  • 摘要:
      目的   探讨SOD活性及SOD基因表达与衰老的相关性。
      方法   选用快速老化模型鼠(SAM-P/8)进行试验, 采用生物化学及RT-PCR方法, 从亚细胞及分子水平上测定衰老过程中丙二醛(MDA), 超氧化物歧化酶(SOD)及其基因表达的变化情况。
      结果   SAM-P/8肝组织中SOD活性随着年龄的增加而逐渐降低; 而MDA含量随年龄的增加逐渐增高, 有显著性差异(P < 0.05)。不同月龄SAM-P/8肝细胞中Cu-Zn SOD基因表达随年龄的增加Cu-Zn SOD mRNA逐渐降低(P < 0.01)。
      结论   SOD活性与SOD基因表达的增龄变化有明显的相关性, SOD基因转录活性的降低可能是导致SOD活性降低的重要原因, 表明SOD在衰老过程中扮演着重要角色。

     

    Abstract:
      Objective   To explore the changes on activity and expression of SOD in the progress of senescence.
      Methods   Senescence-accelerated mouse p/8(SAM-P/8)was used in the study.Biochemical assay and Reverse Transcription Polymerase Chain Reaction(R T-PCR)were adopted to measure changes occurred on SOD activity and gene expression in the progress of senescence.
      Results   Activity of SOD decreased significantly with aging(P < 0.05), while the level of MDA increased significantly with aging(P < 0.05).And the level of Cu-Zn SOD mRNA derived from the liver of SAM-P/8 decreased with aging(P < 0.01).
      Conclusion   Activity of SOD gradually decreased with aging.The decreasing expression of Cu-Zn SOD gene was an important reason for SOD's decreasing activity.It could be inferred that SOD played an important role in the progress of aging.

     

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