增食因子在肥胖抵抗大鼠食欲调节中的作用

刘荣; 孙长颢; 王舒然

增食因子在肥胖抵抗大鼠食欲调节中的作用

Role of orexin in appetite regulation of DIO-R rats

摘要

摘要:
目的探讨增食因子A和B在肥胖抵抗大鼠食欲调节中的作用。
方法 50只健康雄性SD大鼠, 随机分为对照组和高脂组, 分别用基础饲料和高脂饲料喂养13周, 然后根据体重和能量摄入量筛选出饮食诱导肥胖抵抗(diet-induced obesity resistance, DIO-R)和饮食诱导肥胖(diet-induced obesity, DIO)组, 观察摄食量的变化, Western Blot法测定大鼠脑组织中增食因子A和B的蛋白含量。
结果 DIO-R大鼠总摄食量明显低于DIO大鼠(P < 0.05); 高脂饲料可增加大鼠脑组织中增食因子A和B的含量, 但DIO-R大鼠和DIO大鼠间无显著性差异。
结论 DIO-R大鼠体内增食因子A(orexinA)和B(orexinB)的增食作用可能被其它抑制食欲因素的作用所掩盖。因而, orexinA和orexinB在肥胖抵抗大鼠的食欲调节中作用较弱。

Abstract:
Objective In order to explore the role of orexin A, B in appetite regulation of diet-induced obesity resistant(DIO-R)rats.
Methods Fifty male Sprague-dawley(SD)rats were randomly divided into control group and high-fat group and were fed with basic diet and high-fat diet respectively for 13 weeks.Diet-induced obesity resistant and diet-induced obesity rats were selected according to their body weight and the quantity of energy intake, then observing change of the food intake and the content of orexin A, B in brain was determined by Western Blot.
Results The total food intake of DIO-R rats were lower than those of DIO rats(P < 0.05).High fat diets increased the content of orexin A and orexin B of rats, but there was no significant difference between DIO-R and DIO rats.
Conclusion The increasing appetite role of orexin A and orexin B was covered by other anti-appetite factors, also orexin A and orexin B played minor role in appetite regulation of DIO-R rats.

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