硒对环氧化酶2 mRNA表达的影响

Effects of Se on COX-2 mRNA expression

摘要:
目的观察硒对肿瘤发生密切相关的环氧化酶2(COX-2)表达的影响。
方法选用人结肠癌细胞系HT-29, 采用定量PCR方法测定不同剂量(0.25, 0.5, 1.0μmol/L)硒在4, 8和24h对COX-2mRNA表达的影响。
结果硒在0.5和1.0μmol/L剂量作用24h条件下, 对HT-29的COX-2mRNA表达有下调作用, 其中0.50μmol/L是最佳作用剂量。
结论硒通过下调COX-2mRNA表达, 发挥癌症预防作用。为科学饮食预防癌症和将硒作为抗癌药物进行开发提供理论依据。

Abstract:
Objective To investigate effects of Se, in the form of sodium selenite, on COX-2 mRNA expression, a cancer close related gene.
Methods The amount of COX-2 mRNA of HT-29 cells, which were cultured in DMEM containing the concentration of 250, 500 and 1 000 nmol/L Se for 4, 8 and 24 h, was detected by real time PCR.
Results The expression of COX-2 mRNA were down regulated significantly by 500 and 1 000 nmol/L Se for 24 hours.
Conclusion Se had chemoprotective effect on human colon cancer through down regulating COX-2 gene expression and did good to man's suitable diet and offer a background for develoing Se anticancer drug.