血浆醋氯芬酸测定方法的改进

谢虹; 邱相君; 王桂珍

血浆醋氯芬酸测定方法的改进

Modified method for determination of aceclofenac in plasma

摘要

摘要:
目的为提高血浆醋氯芬酸的分离效果, 对高效液相色谱(HPLC)测定方法进行改进。
方法血浆中醋氯芬酸经盐酸酸化后用乙醚萃取浓缩, 以Hypersil-C₁₈(46mm×250mm, 10μm)为色谱柱; 以甲醇: 水: 冰醋酸(70:30:0.5, V/V为流动相, 流速为15ml/min; 检测波长为276nm。
结果醋氯芬酸浓度在0.1~100μg/ml范围内线性关系良好(r=0.9961);最低检出限为0.1μg/ml; 平均回收率为(10117±117)%, 日内、日间精密度分别为(6.04±2.45)%和(5.01±139)%。
结论改进后的方法简便、准确可靠、灵敏, 适用于人血浆醋氯芬酸浓度的测定和药代动力学研究。

Abstract:
Objective A modified method based on high performance liquid chromatography was developed for the determination of aceclofenac in human plasma.
Methods Aceclofenac was extracted from plasma with diethylether.The analytical column was packetd with Hypersil-C₁₈(4.6 mm×250 mm, 10 L).The mobile phase was methanol-water-glacial acetic acid(73B30B0.5, V/V)and the flow rate was 1.5 ml/min.The UV detection wavelength was 276 nm.
Results The calibration curve was liner in the range from 0.1 to 100μg/ml(r=0.996 1), with determination limit of 0.1 μg/ml and the average recovery of(101.17±1.17)%.The intra-day RSD and inter-day RSD were(6.04±2.45)%and(5.01±1.39)%, respectively.
Conclusion The method was simple, rapid, accurate and can be used to determine the aeclofenac concentration in plasma and for pharmacokinetics study.

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