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应晨江, 孙秀发, 左学志, 刘烈钢, 杨年红, 郝丽萍, 杨雪锋. 茶多酚降低血管紧张素Ⅱ诱导的内皮高渗透性[J]. 中国公共卫生, 2004, 20(4): 416-417.
引用本文: 应晨江, 孙秀发, 左学志, 刘烈钢, 杨年红, 郝丽萍, 杨雪锋. 茶多酚降低血管紧张素Ⅱ诱导的内皮高渗透性[J]. 中国公共卫生, 2004, 20(4): 416-417.
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YING Chen-jiang, SUN Xiu-fa, ZHO Xue-zhi, . Tea polyphenols alleviated endothelial hyperpermeability induced by angiotensin Ⅱ[J]. Chinese Journal of Public Health, 2004, 20(4): 416-417.
Citation: YING Chen-jiang, SUN Xiu-fa, ZHO Xue-zhi, . Tea polyphenols alleviated endothelial hyperpermeability induced by angiotensin Ⅱ[J]. Chinese Journal of Public Health, 2004, 20(4): 416-417.
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茶多酚降低血管紧张素Ⅱ诱导的内皮高渗透性

Tea polyphenols alleviated endothelial hyperpermeability induced by angiotensin Ⅱ

    摘要
  • 摘要:
      目的   观察茶多酚对血管紧张素(AngⅡ)诱导的内皮细胞渗透性的影响, 并探讨其作用机制。
      方法   用绿茶茶多酚或红茶茶多酚(0.4mg/L)预培养牛颈动脉内皮细胞(BCAECs)24h后, 加入AngⅡ(10mg/L)刺激, 观察细胞内超氧阴离子(O)的水平及细胞外过氧化氢浓度的变化。另在培养单层细胞中用FITC-dextran(2.5μmol/L)观察漏过荧光量的变化, 评价茶多酚对AngⅡ刺激的内皮细胞渗透性的影响。
      结果   绿茶茶多酚或红茶茶多酚均明显降低AngⅡ刺激的O2-°水平(P < 0.05), 并降低由AngⅡ刺激的渗透性增加(P < 0.05), 这种作用可以用还原型辅酶Ⅱ(NADPH)氧化酶抑制剂DPI和抗氧化物VE预培养来实现。
      结论   茶多酚主要通过抑制AngⅡ-NADPH氧化酶-O2-°信号途径, 降低细胞的渗透性。

     

    Abstract:
      Objective   To observe the effects of tea polyphenols on endothelial cell hyperpermeability induced by Ang Ⅱ and search for the mechanism of tea polyphenols on down-stream redox signal mediated by Ang Ⅱ receptor typeⅠ(AT1).
      Methods   The intracellular superoxide anion production in BACECs challenged with AngⅡ(1 mg/L)was measured by quantitative nitroblue tetrazolium(NBT)assay and the extracellular hydrogen peroxide concentration was determined after the cells were pre-incubated with GTP or BTP(0.4 mg/L)for 24 h.In cultured monolayer BCA ECs, permeable FITC- dextran(2.5μmol/L)was measured to evaluate the effects of tea polyphenols on the changes of AngⅡ-induced endothelial permeability.
      Results   Both green tea and black tea polyphenols diminished the intracellular superoxide anion induced by Ang Ⅱ(P < 0.05), and alleviated the intercellular permeability induced by Ang Ⅱ(P < 0.05).The similar permeability decrease induced by Ang Ⅱ was also obtained using either NADPH oxidase inhibitor DPI or antioxidant VE pretreatment.
      Conclusion   Tea polyphenols alleviated Ang Ⅱ-induced hyperpermeability-mainly via decreasing the ROS production.

     

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