黑米皮抑制apoE基因缺陷小鼠T淋巴细胞表达

Inhibiting function of BRF on Tlymphocyte expression in apolipoprote in E deficient mice

摘要:
目的研究黑米皮对载脂蛋白E(apoE)基因缺陷小鼠主动脉窦CD ₄⁺和CD₈ ⁺T淋巴细胞表达的影响, 探讨黑米皮抗动脉粥样硬化(AS)作用的机制。
方法选用apoE基因缺陷小鼠进行实验, 通过在apoE基因缺陷小鼠普通饲料AIN-93中添加5%黑米皮, 检测apoE基因缺陷小鼠主动脉窦动脉粥样斑块面积和CD ₄ ⁺、CD ₈ ⁺T淋巴细胞表达的水平。
结果黑米皮可降低apoE基因缺陷小鼠主动脉窦动脉粥样斑块面积以及CD ₄ ⁺T淋巴细胞表达的水平。
结论黑米皮的抗AS作用与其抑制CD ₄ ⁺T淋巴细胞表达有关。

Abstract:
Objective To explore the effect of black rice outer layer fraction(BRF)on CD ₄ ⁺ T, CD ₈ ⁺ T lymphocyte expression in apoE-deficient mice and find the possible mechanism of BRF in anti-atherogenesis.
Methods CD ₄ ⁺T and CD ₈ ⁺T lymphocyte expression in aortic sinus and atherosclerotic lesion area in aortic sinus were assessed by immunohistochemistry and image analysis, respectively.
Results BRF inhibited CD ₄ ⁺T lymphocyte expression and atherosclerotic lesion area in aortic sinus.
Conclusion BRF dramatically reduced CD ₄ ⁺T lymphocyte expression and this effect maybe related to its antiatherogenesis.