恙螨体内汉坦病毒核酸的基因芯片检测

朱进; 陶开华; 操敏; 张云; 李越希; 唐家琪

恙螨体内汉坦病毒核酸的基因芯片检测

Detection for hantavirus in chigger mite with microarray techniques

摘要

摘要:
目的建立快速、灵敏、特异的汉坦病毒基因芯片诊断方法。
方法根据发表的汉坦病毒属(HV)76-118株和R22株S基因核苷酸序列, 设计引物和探针, 制备寡核苷酸芯片.用Cy3标记核苷和引物, 运用不对称PCR技术制备单链荧光标记核苷酸片段, 并与芯片上的寡核苷酸探针杂交, 荧光扫描仪检测并分析信号。
结果研究制备的基因芯片能够检测汉坦病毒HTN型和SEO型病毒核酸的特异性荧光信号。
结论 HV的基因芯片检测具有特异、灵敏、快速的优点.基因芯片的制备和检测技术的建立, 可为肾综合征出血热等传染病的诊断和预防提供理想的方法。

Abstract:
Objective To establish a rapid, sensitive and specific diagnostic assay for Hantavirus with microarray techniques.
Methods Specific primers and probes were designed according to the conservative and specific DNA sequence of 76118 strain and R22 strain.The probes were spotted on glass slides to form microarrays.The Cy3-labled single strand DNA fragment prepared by dissymmetical PCR were hybridized with the probers on the glass slides.The microarrays were scanned and analyzed with a scanner.
Results DNA microarray could detect the different typed DNA of HTN and SEO with adequate specificity and sensitivity.
Conclusion The successfully developed DNA microarray and techniques might be a very useful method for diagnosis and prevention, and widely applied in specific pathogen detection of infectious diseases such as hemorrhagic fever with renal syndrome.

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